CN101617048B - 具有优化的5'端前导功能（5'-utr）的人工dna序列用以改进异源蛋白质在植物内的表达 - Google Patents

具有优化的5'端前导功能（5'-utr）的人工dna序列用以改进异源蛋白质在植物内的表达 Download PDF

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CN101617048B
CN101617048B CN2007800518216A CN200780051821A CN101617048B CN 101617048 B CN101617048 B CN 101617048B CN 2007800518216 A CN2007800518216 A CN 2007800518216A CN 200780051821 A CN200780051821 A CN 200780051821A CN 101617048 B CN101617048 B CN 101617048B
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artificial dna
sequence
plants
plant
protein
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CN101617048A (zh
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S·马尔凯蒂
F·德阿米西斯
T·帕蒂
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Adiane Pharmaceutical And Biotechnology Co Ltd
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Universita degli Studi di Udine
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
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  • Medicinal Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Environmental Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CN2007800518216A 2006-12-29 2007-12-27 具有优化的5'端前导功能（5'-utr）的人工dna序列用以改进异源蛋白质在植物内的表达 Expired - Fee Related CN101617048B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
IT000280A ITUD20060280A1 (it) 2006-12-29 2006-12-29 Sequenza artificiale di dna evente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine eterologhe in pianta
ITUD2006A000280 2006-12-29
PCT/EP2007/064590 WO2008080954A1 (en) 2006-12-29 2007-12-27 Artificial dna sequence with optimized leader function in 5' (5'-utr) for the improved expression of heterologous proteins in plants

Publications (2)

Publication Number Publication Date
CN101617048A CN101617048A (zh) 2009-12-30
CN101617048B true CN101617048B (zh) 2012-11-07

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CN2007800518216A Expired - Fee Related CN101617048B (zh) 2006-12-29 2007-12-27 具有优化的5'端前导功能（5'-utr）的人工dna序列用以改进异源蛋白质在植物内的表达

Country Status (18)

Country Link
US (1) US9085775B2 (OSRAM)
EP (1) EP2118291B1 (OSRAM)
JP (2) JP2010514432A (OSRAM)
KR (1) KR101596229B1 (OSRAM)
CN (1) CN101617048B (OSRAM)
AU (1) AU2007341217A1 (OSRAM)
BR (1) BRPI0720641A8 (OSRAM)
CA (1) CA2674170C (OSRAM)
CY (1) CY1116649T1 (OSRAM)
DK (1) DK2118291T3 (OSRAM)
ES (1) ES2546184T3 (OSRAM)
HU (1) HUE027484T2 (OSRAM)
IT (1) ITUD20060280A1 (OSRAM)
NZ (1) NZ578518A (OSRAM)
PL (1) PL2118291T3 (OSRAM)
PT (1) PT2118291E (OSRAM)
RU (1) RU2009129091A (OSRAM)
WO (1) WO2008080954A1 (OSRAM)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ITUD20080055A1 (it) * 2008-03-13 2009-09-14 Transactiva S R L Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
WO2013183863A1 (ko) * 2012-06-04 2013-12-12 대한민국(관리부서:국립수산과학원) ３'영역의 염기 변이를 통한 전체 단백질의 발현 조절 방법
ITUD20130002A1 (it) * 2013-01-16 2014-07-17 Transactiva S R L Sequenza artificiale di dna avente funzione di leader in 5' (5'-utr) ottimizzata per la sovraespressione di proteine ricombinanti in pianta e metodo per la produzione di proteine ricombinanti in pianta
JP6609551B2 (ja) * 2013-06-25 2019-11-20 ザ ユニバーシティー オブ ユタ リサーチ ファンデーション ポリメラーゼ連鎖反応を行う方法及びその関連する使用
IT201700042052A1 (it) * 2017-04-14 2018-10-14 Transactiva S R L Vettore di espressione e metodo per la produzione di una proteina umana in pianta, in particolare un anticorpo ricombinante in endosperma di cereale
CN121022838A (zh) * 2025-02-06 2025-11-28 北京臻知医学科技有限责任公司 一种5’UTR序列及其在提高mRNA翻译效率中的应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8613481D0 (en) * 1986-06-04 1986-07-09 Diatech Ltd Translation of mrna
GB9206016D0 (en) * 1992-03-19 1992-04-29 Sandoz Ltd Improvements in or relating to organic compounds
US5362865A (en) 1993-09-02 1994-11-08 Monsanto Company Enhanced expression in plants using non-translated leader sequences
GB9515941D0 (en) 1995-08-03 1995-10-04 Zeneca Ltd DNA constructs
JP3761628B2 (ja) 1995-07-14 2006-03-29 住友化学株式会社 植物プロモーターおよびその利用
GB9516241D0 (en) 1995-08-08 1995-10-11 Zeneca Ltd Dna constructs
ZA9811228B (en) 1997-12-12 1999-06-14 Mogen Int New constitutive plant promoters
US6376747B1 (en) 1999-08-27 2002-04-23 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture And Agri-Food Canada Plant-derived map kinase kinase
FR2815969B1 (fr) 2000-10-30 2004-12-10 Aventis Cropscience Sa Plantes tolerantes aux herbicides par contournement de voie metabolique
KR100474115B1 (ko) 2001-06-01 2005-03-08 대한민국 유전자변형식물에 대한 ｐｃｒ 검정용 프라이머
US7517689B2 (en) 2004-07-09 2009-04-14 Donald Danforth Plant Science Center Methods and compositions for regulating gene expression in plant cells
US7847064B2 (en) 2004-07-09 2010-12-07 Donald Danforth Plant Science Center Methods and compositions for regulating gene expression in plant cells
US8065093B2 (en) * 2004-10-06 2011-11-22 Agency For Science, Technology, And Research Methods, systems, and compositions for classification, prognosis, and diagnosis of cancers

Also Published As

Publication number Publication date
US20110041222A1 (en) 2011-02-17
KR101596229B1 (ko) 2016-03-07
JP2010514432A (ja) 2010-05-06
KR20090102825A (ko) 2009-09-30
ES2546184T3 (es) 2015-09-21
ITUD20060280A1 (it) 2008-06-30
EP2118291B1 (en) 2015-06-03
CN101617048A (zh) 2009-12-30
CA2674170A1 (en) 2008-07-10
US9085775B2 (en) 2015-07-21
JP5913434B2 (ja) 2016-04-27
JP2014221040A (ja) 2014-11-27
RU2009129091A (ru) 2011-02-10
BRPI0720641A8 (pt) 2017-12-19
CA2674170C (en) 2019-03-12
AU2007341217A1 (en) 2008-07-10
EP2118291A1 (en) 2009-11-18
NZ578518A (en) 2012-08-31
WO2008080954A1 (en) 2008-07-10
CY1116649T1 (el) 2017-03-15
PT2118291E (pt) 2015-09-24
BRPI0720641A2 (pt) 2014-03-18
DK2118291T3 (en) 2015-08-31
PL2118291T3 (pl) 2015-11-30
HUE027484T2 (en) 2016-09-28

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Effective date of registration: 20160324

Address after: The city of Lugano, Switzerland

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Patentee after: Adiane Pharmaceutical and Biotechnology Co., Ltd.

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Granted publication date: 20121107

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