CN101612212A - A kind of application process of natural plant peony extract - Google Patents
A kind of application process of natural plant peony extract Download PDFInfo
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- CN101612212A CN101612212A CN200910055789A CN200910055789A CN101612212A CN 101612212 A CN101612212 A CN 101612212A CN 200910055789 A CN200910055789 A CN 200910055789A CN 200910055789 A CN200910055789 A CN 200910055789A CN 101612212 A CN101612212 A CN 101612212A
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Abstract
The present invention relates to a kind of application process of natural plant peony extract, this application process is that natural plant peony extract is applied to suppress in medicine, cosmetics and the health food of histamine release.The present invention adopts and suppresses the test model that mastocyte discharges histamine, confirms the antiallergic and the antipruritic effect of natural plant peony extract.
Description
Technical field
The present invention relates to a kind of resisting skin allergy, antipruritic effective active composition, this active component is a natural plant peony extract, especially relates to a kind of application process of natural plant peony extract.
Background technology
In recent years Epidemiological study shows that anaphylactic disease has the trend that increases year by year, and particularly the increase trend of anaphylaxis that is caused by environment reason and anti-heritability allergic dermatitis is particularly remarkable, has become very important social phenomenon.The appearance of its problem is not only because the abuse of the additive of the increase year by year of anaphylactogen, atmospheric pollution, foods and cosmetics, and because the variation of people's dietary habit in recent years.The Therapeutic Method of anaphylactic disease is a basis to eliminate anaphylactogen usually, and then carries out the alternative medicine treatment according to degree, cause and the pathogenesis of clinical symptoms.Common anaphylaxis is generally type i allergic reaction, as diseases such as allergic rhinitis, bronchial asthma and urticaria, discharge chemical mediator mediations such as histamine by mast cell degranulation, stimulate vasodilation, increase vascular permeability, promote bronchial smooth muscle to shrink, improve excessive organism stress such as terminal neuroinduction.Therefore, to the treatment of I type anaphylactic disease, mainly use hydryllin clinically and mast cell degranulation is had the active Claritin of inhibition.
Skin pruritus often follows allergic symptom to produce.The receptor of mediation pruritus is positioned on the no marrow C fiber free nerve endings of corium and epidermis junction, connects the secondary neuron by nerve synapse, arrives the flaggy nuclear of thalamus then by spinothalamic tract, arrives cerebral cortex at last, produces the pruritus sensation.In recent years studies confirm that number of chemical medium such as histamine etc. can cause experimental pruritus, and these media produce the gargalesthesia neural impulse by combining with specific receptor on the no marrow C fiber free nerve endings.The mediator that participates in pruritus is many and complicated, but also some common mediator such as histamine, interleukins etc.Wherein property pruritus in histamine source is a most clearly pruritus pathway of research at present.
Antihistaminic is present clinical treatment pruritus and drug of first choice hypersensitive.But present synthetic antihistaminic is most in clinical use side effect such as sleepy, thirsty, gastro-intestinal digestion system damage to occur, takes for a long time also to have certain clinical safety problem.
The antiallergic preparation of clinical practice is confined to temporary alleviation, and can not fundamentally improve allergic constitution.From healthy and living habit angle, a kind of effective and safe be used for antipruritic and antianaphylactic pharmaceuticals, health food or cosmetics are expected by people.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of food seasoning agent that can be used as for the defective that overcomes above-mentioned prior art existence, toxicity is lower, and can under lower concentration, suppress the release of histamine, can well dispel skin pruritus, reach the application process of antiallergic effect natural plant peony extract.
Purpose of the present invention can be achieved through the following technical solutions: a kind of application process of natural plant peony extract, it is characterized in that, this application process is that natural plant peony extract is applied to suppress provide antiallergic, antipruritic effect in medicine, cosmetics or the health food of histamine release.It is 0.001%～100% (W/W) that described Radix Paeoniae Alba extract adds concentration range.
Described natural plant peony extract comprises the thick Radix Paeoniae Alba extract that adopts solvent extraction, steam distillation or carbon dioxide supercritical fluid extraction to obtain, and the refining Radix Paeoniae Alba extract and the monomer whose that adopt process for purification to obtain after the extraction.
Described solvent extraction is natural plant peony to be pulverized to be placed on extract in the solvent, and the mass ratio of Radix Paeoniae and extraction solvent is 2/1-1/40, under 20-100 ℃, soaks 0.1-336h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Described organic solvent is one or more in alcohol, ethyl acetate, dipropylene glycol or the petroleum ether of 1-5 carbon.
Organic solvent is the alcohol of 1-5 carbon or in the dipropylene glycol one or more in the described aqueous solutions of organic solvent.
The alcohol of a described 1-5 carbon comprises ethanol, methanol, glycerol, propylene glycol, 1,3 butylene glycol.
Described carbon dioxide supercritical fluid extraction is natural plant peony to be pulverized be placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 15-45MPa, extraction temperature 30-55 ℃, carbon dioxide flow 8-25kg/h carries out supercritical extraction, extraction time 0.5-4h, separating still is adjusted separating pressure 3-10MPa then, and separation temperature is 30-50 ℃, isolate fluid extract, get Radix Paeoniae Alba extract.
Described steam distillation is that natural plant peony is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, feeding pressure is 0.12MPa～0.15MPa water vapour, distillation time is 0.5-4h, collect the liquid on the water layer in the condenser then, get Radix Paeoniae Alba extract.
Described process for purification be Radix Paeoniae Alba extract that solvent extraction or carbon dioxide supercritical fluid extraction are obtained after non-polar resin absorption, adopt the eluant eluting, be concentrated into extractum, obtain purified Radix Paeoniae Alba extract.
Described eluant is that water or volumetric concentration are 30%～95% aqueous solutions of organic solvent, and organic solvent is ethanol or methanol.
Described eluant preferred water or volumetric concentration are 25%～50% aqueous solutions of organic solvent, the organic solvent preferred alcohol.
Described non-polar resin comprises HPD100, D101, HZ891.
Natural plant peony extract provided by the invention can be put on market by multiple peroral dosage form, is used for improving irritated.Certainly, Radix Paeoniae Alba extract also can combine with pharmaceutical carriers such as excipient commonly used and preparationization.Also can add preparation additives such as some antiseptic, antioxidant, coloring agent, sweeting agent in case of necessity.
Can require according to the psychology of market demand and consumer to change dosage form, for example use dosage forms such as tablet, granule, capsule.
Relatively Shi Yi excipient generally considers to have lactose, white sugar, D-mannitol, starch, crystalline cellulose etc.Lubricant generally should adopt magnesium stearate, calcium stearate, Pulvis Talci etc.Solvent uses Purified Water, ethanol, propylene glycol etc. usually.Antiseptic generally adopts methaform, benzylalcohol, dihydroxy acetic acid etc.Antioxidant is more suitable with sulphite, ascorbic acid etc. usually.
Radix Paeoniae Alba extract provided by the invention also can prototype, aqueous solution dilution back uses as skin preparations for extenal use.Can certainly make certain preparation by combining, the consumption in cosmetics, pharmaceuticals market is provided as dosage forms such as aerosol, liquid preparation, extract, suspensoid, Emulsion, ointment, semifluid preparation, liniment, lotions with pharmaceutical carrier.
The present invention in conjunction with modern sciences such as pharmacology and pharmacodynamicss, sets up a kind of antihistamine of assessing natural plant extracts and discharges model, and on this basis medicine is carried out pharmacology pharmacodynamic and measure on the basis of traditional medicine.Histamine is a kind of medium of itching that causes of classics, mainly is stored in interior mastocyte of body and the alkaline granulocyte, and the factor of any stimulation mastocyte all can make mast cell degranulation discharge histamine as factors such as allergia, physical property, spiritualities, causes pruritus.Therefore have stable mastocyte, the material of minimizing histamine release effect can be used for treating the pruritus by due to the histamine.
Except that specified otherwise, reagent that the present invention relates to and raw material are all commercially available to be got.
Compared with prior art, maximum efficiency of the present invention is: Radix Paeoniae Alba extract of the present invention is except traditional compound recipe, domestic and foreign literature does not all have the single Radix Paeoniae Alba extract can suppress the release of histamine and reaches the report of skin antiallergic effect, and this is the bright spot of maximum of the present invention.Radix Paeoniae Alba extract can be made Chinese medicine preparation, is used for pressing down the histamine release in mastocyte and the alkaline granulocyte, thereby plays the effect of resisting skin allergy.And it is a natural plant extracts, and side effect is little.
The specific embodiment
Further specify the present invention with embodiment below, but the present invention is not limited.
Former medicine among following each embodiment: extracting solution: the ratio of portions of resin eluant is weight ratio.
Embodiment 1
Get Radix Paeoniae 100g, extracting solution A is a pure water, configuration eluant B: deionized water, C:70% ethanol water (V/V) is selected non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 12.
Radix Paeoniae is pulverized the back soak 72h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 2
Get Radix Paeoniae 100g, extracting solution A is a pure water, configuration eluant B: deionized water, C:70% ethanol water (V/V) is selected non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 12.
Radix Paeoniae is pulverized the back extract 5h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 3
Get Radix Paeoniae 100g, extracting solution A is a pure water, configuration eluant B: deionized water, C:70% methanol aqueous solution (V/V) is selected non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 2: 9: 12.
Radix Paeoniae is pulverized the back extract three times at 80 ℃ with extracting solution A, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 4
Get Radix Paeoniae 100g, extracting solution A is a pure water, configuration eluant B: deionized water, C:70% methanol aqueous solution (V/V) is selected non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 2: 12: 8.
Radix Paeoniae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 5
Get Radix Paeoniae 100g, extracting solution A is an ethanol, configuration eluant B: deionized water, C:50% ethanol water (V/V) is selected non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 9.
Radix Paeoniae is pulverized the back extract 48h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 6
Get Radix Paeoniae 100g, extracting solution A is a methanol, configuration eluant B: deionized water, C:50% ethanol water (V/V) is selected non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 2: 12: 8.
Radix Paeoniae is pulverized the back extract 12h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 7
Get Radix Paeoniae 100g, extracting solution A is an ethyl acetate, configuration eluant B: deionized water, C:50% methanol aqueous solution (V/V) is selected non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 3: 12: 12.
Radix Paeoniae is pulverized the back extract three times at 80 ℃ with extracting solution A, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 8
Get Radix Paeoniae 100g, extracting solution A is a petroleum ether, configuration eluant B: deionized water, C:50% methanol aqueous solution (V/V) is selected non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 9.
Radix Paeoniae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 9
Get Radix Paeoniae 100g, configuration extraction liquid A is 85% ethanol water (V/V), configuration eluant B: deionized water, C:30% ethanol water (V/V), select non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 20: 3: 12: 9.
Radix Paeoniae is pulverized the back extract 24h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 10
Get Radix Paeoniae 100g, configuration extraction liquid A is 30% ethanol water (V/V), configuration eluant B: deionized water, C:30% ethanol water (V/V), select non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 9.
Radix Paeoniae is pulverized the back extract 8h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 11
Get Radix Paeoniae 100g, extracting solution A is 75% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:30% methanol aqueous solution (V/V), select non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 12.
Radix Paeoniae is pulverized the back extract twice with extracting solution A at 80 ℃, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Embodiment 12
Get Radix Paeoniae 100g, extracting solution A is 50% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:30% methanol aqueous solution (V/V), select non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 12.
Radix Paeoniae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Radix Paeoniae Alba extract.
Effect embodiment 1 Radix Paeoniae Alba extract suppresses the histamine release experiment
Radix Paeoniae Alba extract is by embodiment 1 preparation, and it is as follows to measure the histamine release method:
1. mastocyte separation and purification
The method of employing Anna Nemeth etc. is improved slightly, and cervical vertebra execution is taken off in the normal SD rats blood-letting, and the PBS of lumbar injection pre-cooling (containing 1%FCS) 15ml massages abdominal part 2min gently, draw peritoneal lavage fluid also to insert in the Tissue Culture Dish, and in 37 ℃, 5%CO
2Cell culture incubator leaves standstill 30min, waits for behind the attached wall of macrophage that centrifugal (4 ℃, 1500r/min) 10min regulates the about 0.5ml of cell suspension to gentle aspiration cell suspension.7ml P-F10 (Percoll-HamF10) is at 4 ℃, the centrifugal 20min of 3000r/min forms gradient, spissated cell suspension is spread on the gradient 4 ℃ gently, the centrifugal 20min isolated cell of 2000r/min is collected 1/3 cell under the interface, with 2 (1000r/min of PBS washing, centrifugal 10min) back is resuspended, trypan blue staining detects cell viability, and Toluidine blue staining detects cell purity, purity greater than 95% mastocyte.Regulate cell density about 10
6/ ml places the DMEM culture medium that contains 10%FCS stand-by.
2. drug treating
Mastocyte is adjusted into contains RPMCs 5 * 10 approximately
4Individual/group, the Radix Paeoniae extracting solution that adds variable concentrations, insert 37 ℃, hatched in the 5%CO2 incubator 20 minutes, and added compound48/80 (0.5ug/ml) after the taking-up immediately, insert 37 ℃, hatched in the 5%CO2 incubator 10 minutes, take out and put into ice-water bath 5min cessation reaction immediately, model control group replaces medicine with PBS, and the normal control group is not for adding medicine and compound48/80 processed group.
3. sample is prepared
With 4 ℃, centrifugal 10 minutes of 6000r/min draws supernatant and measures the histamine amount A that is discharged into outside the born of the same parents with each pipe; Precipitation is resuspended with PBS, and 100 ℃ are boiled the 10min cell lysis, measures to remain in intracellular histamine amount A.
4. the mensuration of histamine
Above-mentioned each sample adds perchloric acid (0.4mol/L), centrifugal (4 ℃ 6000r/min) were got supernatant in 10 minutes.Fluoremetry improved methods such as employing Shore are measured: get the 1.6ml supernatant and place the tool plug test tube that adds 1.5g NaCl, add 4.0ml n-butyl alcohol and 0.2ml 2.5N NaOH again, mixing immediately, put Kang Shi oscillator concussion 5 minutes, take out the 3.6ml n-butyl alcohol behind the standing demix in the 10ml tool plug test tube that adds 1.2ml0.1N HCL and 2.0ml normal heptane, shook 5 minutes, abandon organic facies, get 1.0ml HCL in test tube, with one times of tri-distilled water dilution, add 0.5ml 0.4N NaOH alkalization, mixing also adds 0.1%OPT methanol solution 0.1ml rapidly, mixing and 21-22 ℃ of reaction 10 minutes adds 0.5ml0.5N HCL acidify and stops condensation reaction, and makes fluorescent material stable immediately.
Each data is the average of 3 parallel multiple pipes.According to the fluorescence intensity that records, be calculated as follows histamine release rate (P release) and the histamine release suppression ratio (P inhibition) of respectively organizing cell respectively.
P discharges=(the spontaneous secretion histamine of supernatant histamine amount A-amount)/(histamine amount B in the supernatant histamine amount A+ cell) * 100%;
P inhibition=(1-medicine group histamine release amount/model group histamine release amount) * 100%.
Histamine amount in wherein spontaneous secretion histamine amount=normal cell supernatant.
Each organizes experimental data, and (variance analysis is used in ± S) expression, and each experimental group and matched group are compared, and there is the significance meaning P＜0.05 for difference, and there is the highly significant meaning P＜0.01 for difference with mean ± standard deviation.
Discharge model by antihistamine and record example 1 preparation Radix Paeoniae Alba extract histamine release dose-effect relationship such as table 1:
Table 1-embodiment 1 Radix Paeoniae Alba extract histamine release rate
As shown in Table 1, have the effect that suppresses histamine release by the Radix Paeoniae Alba extract of embodiment 1 preparation when the concentration 3.125ug/ml-50ug/ml, when 50ug/ml, the histamine release rate only is 6.54%.Above data P＜0.05 has the significance meaning.
Effect embodiment 2 Radix Paeoniae Alba extract suppress the histamine release experiment
Radix Paeoniae Alba extract is by embodiment 11 preparation, and except the medicine that adds has changed embodiment 11 preparations into, all the other determination steps are with effect embodiment 1.
Discharge model by antihistamine and record example 11 preparation Radix Paeoniae Alba extract inhibition histamine release dose-effect relationship such as tables 2:
Table 2-embodiment 11 Radix Paeoniae Alba extract histamine release rates
As shown in Table 2, have the effect that suppresses histamine release by the Radix Paeoniae Alba extract of embodiment 11 preparation when the concentration 3.125ug/ml-50ug/ml, when 50ug/ml, the histamine release rate is 7.21%.
Embodiment 13
The natural plant peony pulverizing is placed in the water, the mass ratio of Radix Paeoniae and water is 2/1, under 100 ℃, soak 0.1h, filter, filtrate concentrating obtained the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HPD100 absorption, adopting volumetric concentration is that 30% ethanol water is that eluant carries out eluting, is concentrated into extractum, obtains Radix Paeoniae Alba extract.
Embodiment 14
The natural plant peony pulverizing is placed in the organic solvent ethanol, the alcoholic acid mass ratio of Radix Paeoniae and organic solvent is 1/40, under 20 ℃, soak 72h, filter, filtrate concentrating obtained the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HZ891 absorption, adopting volumetric concentration is that 50% ethanol water is that eluant carries out eluting, is concentrated into extractum, obtains Radix Paeoniae Alba extract.
Embodiment 15
The natural plant peony pulverizing is placed in the ethanol water, alcoholic acid volumetric concentration is 10%, the mass ratio of Radix Paeoniae and ethanol water is 1/6, under 30 ℃, soaks 20h, filter, filtrate concentrated obtain the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HZ891 absorption, and adopting volumetric concentration is that 30% methanol aqueous solution is that eluant carries out eluting, be concentrated into extractum, obtain Radix Paeoniae Alba extract.
Embodiment 16
The natural plant peony pulverizing is placed in the methanol aqueous solution, the volumetric concentration of methanol is 95%, the mass ratio of Radix Paeoniae and methanol aqueous solution is 1/8, under 90 ℃, soaks 60h, filter, filtrate concentrated obtain the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin D101 absorption, and adopting volumetric concentration is that 95% methanol aqueous solution is that eluant carries out eluting, be concentrated into extractum, obtain Radix Paeoniae Alba extract.
Embodiment 17
The natural plant peony pulverizing is placed in the methanol aqueous solution, and the volumetric concentration of methanol is 85%, and the mass ratio of Radix Paeoniae and methanol aqueous solution is 1/20, under 90 ℃, soaks 60h, filters, and obtains Radix Paeoniae Alba extract.
Embodiment 18
The natural plant peony pulverizing is placed in the ethanol water, and alcoholic acid volumetric concentration is 70%, and the mass ratio of Radix Paeoniae and ethanol water is 1/30, under 50 ℃, soaks 36h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Embodiment 19
The natural plant peony pulverizing is placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 15MPa, 30 ℃ of extraction temperature, carbon dioxide flow 25kg/h carries out supercritical extraction, extraction time 4h, separating still is adjusted separating pressure 3MPa then, and separation temperature is 50 ℃, isolate fluid extract, get Radix Paeoniae Alba extract.
Embodiment 20
The natural plant peony pulverizing is placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 45MPa, 55 ℃ of extraction temperature, carbon dioxide flow 8kg/h carries out supercritical extraction, extraction time 0.5h, separating still is adjusted separating pressure 10MPa then, separation temperature is 30 ℃, isolate fluid extract, with the fluid extract volumetric concentration is that 75% ethanol is adjusted to the solution that crude drug concentration is 1mg/ml, and this solution is after non-polar resin HZ891 absorption, and adopting volumetric concentration is that 50% ethanol water is that eluant carries out eluting, be concentrated into extractum, obtain Radix Paeoniae Alba extract.
Embodiment 21
Natural plant peony is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, feeding pressure is the 0.12MPa water vapour, and distillation time is 4h, collects the liquid on the water layer in the condenser then, gets Radix Paeoniae Alba extract.
Embodiment 22
Described steam distillation is that natural plant peony is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, feeding pressure is the 0.15MPa water vapour, distillation time is 0.5h, collect the liquid on the water layer in the condenser then, get Radix Paeoniae Alba extract, wherein contain paeonol.
Embodiment 23
The natural plant peony pulverizing is placed in the ethanol water, and alcoholic acid volumetric concentration is 70%, and the mass ratio of Radix Paeoniae and ethanol water is 1/30, under 70 ℃, extracts 4h, filters, and concentrates, and drying gets Radix Paeoniae Alba extract, wherein contains peoniflorin.
Embodiment 24
The natural plant peony pulverizing is placed in the glycerine water solution, and the volumetric concentration of glycerol is 10%, and the mass ratio of Radix Paeoniae and glycerine water solution is 1/40, under 100 ℃, extracts 1h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Embodiment 25
The natural plant peony pulverizing is placed in the aqueous solution of propylene glycol, and the volumetric concentration of propylene glycol is 1%, and the mass ratio of Radix Paeoniae and aqueous solution of propylene glycol is 1/20, under 95 ℃, extracts 2h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Embodiment 26
The natural plant peony pulverizing is placed in the 1,3 butylene glycol aqueous solution, and the volumetric concentration of 1,3 butylene glycol is 30%, and the mass ratio of Radix Paeoniae and 1,3 butylene glycol aqueous solution is 1/10, under 90 ℃, extracts 3h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Embodiment 27
The natural plant peony pulverizing is placed in the dipropylene glycol aqueous solution, and the volumetric concentration of dipropylene glycol is 15%, and the mass ratio of Radix Paeoniae and dipropylene glycol aqueous solution is 1/40, under 85 ℃, extracts 6h, filters, and concentrates, and gets Radix Paeoniae Alba extract.
Embodiment 28
The natural plant peony pulverizing is placed in the 1,3 butylene glycol, and the mass ratio of Radix Paeoniae and 1,3 butylene glycol is 1/10, under 90 ℃, extracts 3h, filters, and gets Radix Paeoniae Alba extract.
Embodiment 29
The natural plant peony pulverizing is placed in the propylene glycol, and the mass ratio of Radix Paeoniae and propylene glycol is 1/5, under 90 ℃, extracts 3h, filters, and gets Radix Paeoniae Alba extract.
Embodiment 30
The natural plant peony pulverizing is placed in the glycerol, and the mass ratio of Radix Paeoniae and glycerol is 1/10, under 90 ℃, extracts 3h, filters, and gets Radix Paeoniae Alba extract.
Embodiment 31
The natural plant peony pulverizing is placed in the dipropylene glycol, and the mass ratio of Radix Paeoniae and dipropylene glycol is 1/10, under 70 ℃, extracts 10h, filters, and gets Radix Paeoniae Alba extract.
Embodiment 32
Natural plant peony pulverized to be placed on dipropylene glycol, propylene glycol, glycerol, 1,3 butylene glycol volume ratio be 1: 1: 1: in 1 the mixed liquor, the mass ratio of Radix Paeoniae and mixed liquor is 1/10, under 70 ℃, extracts 10h, filters, must Radix Paeoniae Alba extract.
Embodiment 33
Natural plant peony pulverized to be placed on dipropylene glycol, propylene glycol, glycerol, 1,3 butylene glycol, water volume ratio be 1: 1: 1: in 1: 1 the mixed liquor, the mass ratio of Radix Paeoniae and mixed liquor is 1/10, under 70 ℃, extracts 10h, filters, must Radix Paeoniae Alba extract.
Embodiment 34
The pulverizing of 100g natural plant peony is placed in the water, and the mass ratio of Radix Paeoniae and water is 1/10, under 70 ℃, extracts 10h, filter, simmer down to extractum, it is in 70% the ethanol water that extractum is added the 1000g volumetric concentration, precipitation, filter, concentrate, get Radix Paeoniae Alba extract.
Embodiment 35
The 100g natural plant peony pulverized to be placed on volumetric concentration be that the mass ratio of Radix Paeoniae and ethanol water is 1/10 in 70% the ethanol water, under 70 ℃, extraction 10h, filter, simmer down to extractum adds extractum in the 1000g water precipitation, filter, concentrate, get Radix Paeoniae Alba extract.
The extract that adopts the foregoing description method to obtain is applied to suppress in medicine, cosmetics or the health food of histamine release, thereby it is antipruritic to reach skin, anti-allergic effects, extract comprises purified Radix Paeoniae Alba extract or Radix Paeoniae Alba extract monomer after solvent extraction, carbon dioxide supercritical fluid extraction, the extraction, and the Radix Paeoniae Alba extract monomer comprises peoniflorin, paeonol or the like.
Claims (8)
1. the application process of a natural plant peony extract, it is characterized in that, this application process is that natural plant peony extract is applied to suppress in medicine, cosmetics and the health food of histamine release, antiallergic is provided, antipruritic effect, it is 0.001%～100% (W/W) that described Radix Paeoniae Alba extract adds concentration range.
2. the application process of a kind of natural plant peony extract according to claim 1, it is characterized in that, described natural plant peony extract comprises the thick Radix Paeoniae Alba extract that adopts solvent extraction, steam distillation or carbon dioxide supercritical fluid extraction to obtain, and the refining Radix Paeoniae Alba extract and the monomer whose that adopt process for purification to obtain after the extraction.
3. the application process of a kind of natural plant peony extract according to claim 2, it is characterized in that, described solvent extraction is natural plant peony to be pulverized to be placed on extract in the solvent, Radix Paeoniae is 2/1-1/40 with the mass ratio that extracts solvent, under 20-100 ℃, soak 0.1-336h, filter, concentrate, get Radix Paeoniae Alba extract.
4. the application process of a kind of natural plant peony extract according to claim 3 is characterized in that, described extraction solvent is water, organic solvent or aqueous solutions of organic solvent.
5. the application process of a kind of natural plant peony extract according to claim 4 is characterized in that, described organic solvent is one or more in alcohol, ethyl acetate, dipropylene glycol or the petroleum ether of 1-5 carbon.
6. the application process of a kind of natural plant peony extract according to claim 4 is characterized in that, organic solvent is the alcohol of 1-5 carbon or in the dipropylene glycol one or more in the described aqueous solutions of organic solvent.
7. according to the application process of claim 5 or 6 described a kind of natural plant peony extracts, it is characterized in that the alcohol of a described 1-5 carbon comprises ethanol, methanol, glycerol, propylene glycol, 1,3 butylene glycol.
8. the application process of a kind of natural plant peony extract according to claim 1 is characterized in that, described medicine, cosmetics and health food are respectively the receivable form of medicine, cosmetics and health food.
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| CN105582104A (en) * | 2015-12-30 | 2016-05-18 | 中国中医科学院中医临床基础医学研究所 | Traditional Chinese medicine composition for treating rheumatoid arthritis |
| CN106389170A (en) * | 2016-11-30 | 2017-02-15 | 广州赛莱拉干细胞科技股份有限公司 | Antiallergic paeonia albiflora cosmetic composition |
| CN106821859A (en) * | 2017-03-16 | 2017-06-13 | 上海芝映生物科技有限公司 | A kind of preparation method of the composite plant extract liquid with antiallergic itching-relieving action |
| CN108159066A (en) * | 2018-02-02 | 2018-06-15 | 昆明理工大学 | The new application of Paeoniflorin |
| CN110272459A (en) * | 2019-07-24 | 2019-09-24 | 中国药科大学 | Two kinds of noval chemical compounds and its antioxidant activity position in root of Paeonia sinjiangensis |
| CN117860642A (en) * | 2024-03-12 | 2024-04-12 | 珠海金肽生物科技有限公司 | Peptide-containing composition for repairing multiple skin barriers, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105582104A (en) * | 2015-12-30 | 2016-05-18 | 中国中医科学院中医临床基础医学研究所 | Traditional Chinese medicine composition for treating rheumatoid arthritis |
| CN106389170A (en) * | 2016-11-30 | 2017-02-15 | 广州赛莱拉干细胞科技股份有限公司 | Antiallergic paeonia albiflora cosmetic composition |
| CN106389170B (en) * | 2016-11-30 | 2019-06-11 | 广州赛莱拉干细胞科技股份有限公司 | A kind of Chinese herbaceous peony Anti-allergic cosmetic composition |
| CN106821859A (en) * | 2017-03-16 | 2017-06-13 | 上海芝映生物科技有限公司 | A kind of preparation method of the composite plant extract liquid with antiallergic itching-relieving action |
| CN106821859B (en) * | 2017-03-16 | 2022-06-17 | 上海芝映生物科技有限公司 | Preparation method of composite plant extract with anti-allergy and itching-relieving effects |
| CN108159066A (en) * | 2018-02-02 | 2018-06-15 | 昆明理工大学 | The new application of Paeoniflorin |
| CN110272459A (en) * | 2019-07-24 | 2019-09-24 | 中国药科大学 | Two kinds of noval chemical compounds and its antioxidant activity position in root of Paeonia sinjiangensis |
| CN117860642A (en) * | 2024-03-12 | 2024-04-12 | 珠海金肽生物科技有限公司 | Peptide-containing composition for repairing multiple skin barriers, and preparation method and application thereof |
| CN117860642B (en) * | 2024-03-12 | 2024-06-04 | 珠海金肽生物科技有限公司 | Peptide-containing composition for repairing multiple skin barriers, and preparation method and application thereof |
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Application publication date: 20091230 |