CN101612292A - A kind of application process of natural plants Rhizoma Smilacis Glabrae extract - Google Patents
A kind of application process of natural plants Rhizoma Smilacis Glabrae extract Download PDFInfo
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Abstract
The present invention relates to a kind of application process of natural plants Rhizoma Smilacis Glabrae extract, this application process is that the natural plants Rhizoma Smilacis Glabrae extract is applied to suppress in medicine, cosmetics and the health food of histamine release.The present invention adopts and suppresses the test model that mastocyte discharges histamine, confirms the antiallergic and the antipruritic effect of natural plants Rhizoma Smilacis Glabrae extract.
Description
Technical field
The present invention relates to a kind of resisting skin allergy, antipruritic effective active composition, this active component is the natural plants Rhizoma Smilacis Glabrae extract, especially relates to a kind of application process of natural plants Rhizoma Smilacis Glabrae extract.
Background technology
In recent years Epidemiological study shows that anaphylactic disease has the trend that increases year by year, and particularly the increase trend of anaphylaxis that is caused by environment reason and anti-heritability allergic dermatitis is particularly remarkable, has become very important social phenomenon.The appearance of its problem is not only because the abuse of the additive of the increase year by year of anaphylactogen, atmospheric pollution, foods and cosmetics, and because the variation of people's dietary habit in recent years.The Therapeutic Method of anaphylactic disease is a basis to eliminate anaphylactogen usually, and then carries out the alternative medicine treatment according to degree, cause and the pathogenesis of clinical symptoms.Common anaphylaxis is generally type i allergic reaction, as diseases such as allergic rhinitis, bronchial asthma and urticaria, discharge chemical mediator mediations such as histamine by mast cell degranulation, stimulate vasodilation, increase vascular permeability, promote bronchial smooth muscle to shrink, improve excessive organism stress such as terminal neuroinduction.Therefore, to the treatment of I type anaphylactic disease, mainly use hydryllin clinically and mast cell degranulation is had the active Claritin of inhibition.
Skin pruritus often follows allergic symptom to produce.The receptor of mediation pruritus is positioned on the no marrow C fiber free nerve endings of corium and epidermis junction, connects the secondary neuron by nerve synapse, arrives the flaggy nuclear of thalamus then by spinothalamic tract, arrives cerebral cortex at last, produces the pruritus sensation.In recent years studies confirm that number of chemical medium such as histamine etc. can cause experimental pruritus, and these media produce the gargalesthesia neural impulse by combining with specific receptor on the no marrow C fiber free nerve endings.The mediator that participates in pruritus is many and complicated, but also some common mediator such as histamine, interleukins etc.Wherein property pruritus in histamine source is a most clearly pruritus pathway of research at present.
Antihistaminic is present clinical treatment pruritus and drug of first choice hypersensitive.But present synthetic antihistaminic is most in clinical use side effect such as sleepy, thirsty, gastro-intestinal digestion system damage to occur, takes for a long time also to have certain clinical safety problem.
The antiallergic preparation of clinical practice is confined to temporary alleviation, and can not fundamentally improve allergic constitution.From healthy and living habit angle, a kind of effective and safe be used for antipruritic and antianaphylactic pharmaceuticals, health food or cosmetics are expected by people.
Summary of the invention
Purpose of the present invention is exactly to provide a kind of food seasoning agent that can be used as for the defective that overcomes above-mentioned prior art existence, toxicity is lower, and can under lower concentration, suppress the release of histamine, can well dispel skin pruritus, reach the application process of antiallergic effect natural plants Rhizoma Smilacis Glabrae extract.
Purpose of the present invention can be achieved through the following technical solutions: a kind of application process of natural plants Rhizoma Smilacis Glabrae extract, it is characterized in that, this application process is that the natural plants Rhizoma Smilacis Glabrae extract is applied to suppress provide antiallergic, antipruritic effect in medicine, cosmetics or the health food of histamine release.It is 0.001%～100% (W/W) that described Rhizoma Smilacis Glabrae extract adds concentration range.
Described natural plants Rhizoma Smilacis Glabrae extract comprises the thick Rhizoma Smilacis Glabrae extract that adopts solvent extraction, steam distillation or carbon dioxide supercritical fluid extraction to obtain, and the refining Rhizoma Smilacis Glabrae extract that adopts process for purification to obtain after the extraction.
Described solvent extraction is the natural plants Rhizoma Smilacis Glabrae to be pulverized to be placed on extract in the solvent, and the mass ratio of Rhizoma Smilacis Glabrae and extraction solvent is 2/1-1/40, under 20-100 ℃, soaks 0.1-336h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Described extraction solvent is water, organic solvent or aqueous solutions of organic solvent.
Described organic solvent is one or more in alcohol, ethyl acetate, dipropylene glycol or the petroleum ether of 1-5 carbon.
Organic solvent is the alcohol of 1-5 carbon or in the dipropylene glycol one or more in the described aqueous solutions of organic solvent.
The alcohol of a described 1-5 carbon comprises ethanol, methanol, glycerol, propylene glycol, 1,3 butylene glycol.
Described carbon dioxide supercritical fluid extraction is the natural plants Rhizoma Smilacis Glabrae to be pulverized be placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 15-45MPa, extraction temperature 30-55 ℃, carbon dioxide flow 8-25kg/h carries out supercritical extraction, extraction time 0.5-4h, separating still is adjusted separating pressure 3-10MPa then, and separation temperature is 30-50 ℃, isolate fluid extract, get Rhizoma Smilacis Glabrae extract.
Described steam distillation is that the natural plants Rhizoma Smilacis Glabrae is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, feeding pressure is 0.12MPa～0.15MPa water vapour, distillation time is 0.5-4h, collect the liquid on the water layer in the condenser then, get Rhizoma Smilacis Glabrae extract.
Described process for purification be Rhizoma Smilacis Glabrae extract that solvent extraction or carbon dioxide supercritical fluid extraction are obtained after non-polar resin absorption, adopt the eluant eluting, be concentrated into extractum, obtain purified Rhizoma Smilacis Glabrae extract.
Described eluant is that water or volumetric concentration are 30%～95% aqueous solutions of organic solvent, and organic solvent is ethanol or methanol.
Described eluant preferred water or volumetric concentration are 25%～50% aqueous solutions of organic solvent, the organic solvent preferred alcohol.
Described non-polar resin comprises HPD100, D101, HZ891.
Natural plants Rhizoma Smilacis Glabrae extract provided by the invention can be put on market by multiple peroral dosage form, is used for improving irritated.Certainly, Rhizoma Smilacis Glabrae extract also can combine with pharmaceutical carriers such as excipient commonly used and preparationization.Also can add preparation additives such as some antiseptic, antioxidant, coloring agent, sweeting agent in case of necessity.
Can require according to the psychology of market demand and consumer to change dosage form, for example use dosage forms such as tablet, granule, capsule.
Relatively Shi Yi excipient generally considers to have lactose, white sugar, D-mannitol, starch, crystalline cellulose etc.Lubricant generally should adopt magnesium stearate, calcium stearate, Pulvis Talci etc.Solvent uses Purified Water, ethanol, propylene glycol etc. usually.Antiseptic generally adopts methaform, benzylalcohol, dihydroxy acetic acid etc.Antioxidant is more suitable with sulphite, ascorbic acid etc. usually.
Rhizoma Smilacis Glabrae extract provided by the invention also can prototype, aqueous solution dilution back uses as skin preparations for extenal use.Can certainly make certain preparation by combining, the consumption in cosmetics, pharmaceuticals market is provided as dosage forms such as aerosol, liquid preparation, extract, suspensoid, Emulsion, ointment, semifluid preparation, liniment, lotions with pharmaceutical carrier.
The present invention in conjunction with modern sciences such as pharmacology and pharmacodynamicss, sets up a kind of antihistamine of assessing natural plant extracts and discharges model, and on this basis medicine is carried out pharmacology pharmacodynamic and measure on the basis of traditional medicine.Histamine is a kind of medium of itching that causes of classics, mainly is stored in interior mastocyte of body and the alkaline granulocyte, and the factor of any stimulation mastocyte all can make mast cell degranulation discharge histamine as factors such as allergia, physical property, spiritualities, causes pruritus.Therefore have stable mastocyte, the material of minimizing histamine release effect can be used for treating the pruritus by due to the histamine.
Except that specified otherwise, reagent that the present invention relates to and raw material are all commercially available to be got.
Compared with prior art, maximum efficiency of the present invention is: Rhizoma Smilacis Glabrae extract of the present invention is except traditional compound recipe, domestic and foreign literature does not all have the single Rhizoma Smilacis Glabrae extract can suppress the release of histamine and reaches the report of skin antiallergic effect, and this is the bright spot of maximum of the present invention.Rhizoma Smilacis Glabrae extract can be made Chinese medicine preparation, is used for pressing down the histamine release in mastocyte and the alkaline granulocyte, thereby plays the effect of resisting skin allergy.And it is a natural plant extracts, and side effect is little.
The specific embodiment
Further specify the present invention with embodiment below, but the present invention is not limited.
Former medicine among following each embodiment: extracting solution: the ratio of portions of resin eluant is weight ratio.
Embodiment 1
The Poria 100g that fetches earth, extracting solution A is a pure water, configuration eluant B: deionized water, C:30% ethanol water (V/V) is selected non-polar resin: HZ891, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 9.
Rhizoma Smilacis Glabrae is pulverized the back soak 72h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HZ891 absorption, discard, collect eluant C eluting section with eluant behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 2
The Poria 100g that fetches earth, extracting solution A is a pure water, configuration eluant B: deionized water, C:30% ethanol water (V/V) is selected non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract 5h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 3
The Poria 100g that fetches earth, extracting solution A is a pure water, configuration eluant B: deionized water, C:30% methanol aqueous solution (V/V) is selected non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 2: 9: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract three times at 80 ℃ with extracting solution A, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 4
The Poria 100g that fetches earth, extracting solution A is a pure water, configuration eluant B: deionized water, C:30% methanol aqueous solution (V/V) is selected non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 2: 12: 8.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 5
The Poria 100g that fetches earth, extracting solution A is an ethanol, configuration eluant B: deionized water, C:50% ethanol water (V/V) is selected non-polar resin: HZ891, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract 48h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HZ891 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 6
The Poria 100g that fetches earth, extracting solution A is a methanol, configuration eluant B: deionized water, C:50% ethanol water (V/V) is selected non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 2: 12: 8.
Rhizoma Smilacis Glabrae is pulverized the back extract 12h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 7
The Poria 100g that fetches earth, extracting solution A is an ethyl acetate, configuration eluant B: deionized water, C:50% methanol aqueous solution (V/V) is selected non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 3: 12: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract three times at 80 ℃ with extracting solution A, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 8
The Poria 100g that fetches earth, extracting solution A is a petroleum ether, configuration eluant B: deionized water, C:50% methanol aqueous solution (V/V) is selected non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 12: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 9
Poria 100g fetches earth, configuration extraction liquid A is 30% ethanol water (V/V), configuration eluant B: deionized water, C:75% ethanol water (V/V), select non-polar resin: HZ891, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 20: 3: 12: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract 24h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HZ891 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 10
Poria 100g fetches earth, configuration extraction liquid A is 50% ethanol water (V/V), configuration eluant B: deionized water, C:75% ethanol water (V/V), select non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract 8h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 11
Poria 100g fetches earth, extracting solution A is 75% ethanol water (V/V), configuration eluant B: deionized water, C:75% methanol aqueous solution (V/V), select non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 80 ℃, each 2h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 12
Poria 100g fetches earth, extracting solution A is 85% ethanol water (V/V), configuration eluant B: deionized water, C:75% methanol aqueous solution (V/V), select non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 13
Poria 100g fetches earth, configuration extraction liquid A is 30% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:95% ethanol water (V/V), select non-polar resin: HZ891, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 20: 3: 12: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract 72h at 30 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HZ891 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 14
Poria 100g fetches earth, configuration extraction liquid A is 50% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:95% ethanol water (V/V), select non-polar resin: D101, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract 8h at 50 ℃, be concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after D101 absorption, discard, collect eluant C eluting section with eluant B behind the B eluting with extracting solution A.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 15
Poria 100g fetches earth, extracting solution A is 75% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:95% methanol aqueous solution (V/V), select non-polar resin: H103, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 10: 3: 9: 12.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after H103 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Embodiment 16
Poria 100g fetches earth, extracting solution A is 85% methanol aqueous solution (V/V), configuration eluant B: deionized water, C:95% methanol aqueous solution (V/V), select non-polar resin: HPD100, former medicine: extracting solution: portions of resin eluant B: eluant C=1: 8: 3: 9: 9.
Rhizoma Smilacis Glabrae is pulverized the back extract twice with extracting solution A at 70 ℃, each 3h is concentrated to the medicinal liquid that is about 1mg/ml crude drug concentration, after HPD100 absorption, discards with eluant B behind the B eluting, collects eluant C eluting section.Be concentrated into extractum, get Rhizoma Smilacis Glabrae extract.
Effect embodiment 1 Rhizoma Smilacis Glabrae extract suppresses the histamine release experiment
Rhizoma Smilacis Glabrae extract is by embodiment 3 preparations, and it is as follows to measure the histamine release method:
1. mastocyte separation and purification
The method of employing Anna Nemeth etc. is improved slightly, and cervical vertebra execution is taken off in the normal SD rats blood-letting, and the PBS of lumbar injection pre-cooling (containing 1%FCS) 15ml massages abdominal part 2min gently, draw peritoneal lavage fluid also to insert in the Tissue Culture Dish, and in 37 ℃, 5%CO
2Cell culture incubator leaves standstill 30min, waits for behind the attached wall of macrophage that centrifugal (4 ℃, 1500r/min) 10min regulates the about 0.5ml of cell suspension to gentle aspiration cell suspension.7ml P-F10 (Percoll-HamF10) is at 4 ℃, the centrifugal 20min of 3000r/min forms gradient, spissated cell suspension is spread on the gradient 4 ℃ gently, the centrifugal 20min isolated cell of 2000r/min is collected 1/3 cell under the interface, with 2 (1000r/min of PBS washing, centrifugal 10min) back is resuspended, trypan blue staining detects cell viability, and Toluidine blue staining detects cell purity, purity greater than 95% mastocyte.Regulate cell density about 10
6/ ml places the DMEM culture medium that contains 10%FCS stand-by.
2. drug treating
Mastocyte is adjusted into contains RPMCs 5 * 10 approximately
4Individual/group, the Rhizoma Smilacis Glabrae extracting solution that adds variable concentrations, insert 37 ℃, hatched in the 5%CO2 incubator 20 minutes, and added compound48/80 (0.5ug/ml) after the taking-up immediately, insert 37 ℃, hatched in the 5%CO2 incubator 10 minutes, take out and put into ice-water bath 5min cessation reaction immediately, model control group replaces medicine with PBS, and the normal control group is not for adding medicine and compound48/80 processed group.
3. sample is prepared
With 4 ℃, centrifugal 10 minutes of 6000r/min draws supernatant and measures the histamine amount A that is discharged into outside the born of the same parents with each pipe; Precipitation is resuspended with PBS, and 100 ℃ are boiled the 10min cell lysis, measures to remain in intracellular histamine amount A.
4. the mensuration of histamine
Above-mentioned each sample adds perchloric acid (0.4mol/L), centrifugal (4 ℃ 6000r/min) were got supernatant in 10 minutes.Fluoremetry improved methods such as employing Shore are measured: get the 1.6ml supernatant and place the tool plug test tube that adds 1.5g NaCl, add 4.0ml n-butyl alcohol and 0.2ml 2.5N NaOH again, mixing immediately, put Kang Shi oscillator concussion 5 minutes, take out the 3.6ml n-butyl alcohol behind the standing demix in the 10ml tool plug test tube that adds 1.2ml0.1N HCL and 2.0ml normal heptane, shook 5 minutes, abandon organic facies, get 1.0ml HCL in test tube, with one times of tri-distilled water dilution, add 0.5ml 0.4N NaOH alkalization, mixing also adds 0.1%OPT methanol solution 0.1ml rapidly, mixing and 21-22 ℃ of reaction 10 minutes adds 0.5ml0.5N HCL acidify and stops condensation reaction, and makes fluorescent material stable immediately.
Each data is the average of 3 parallel multiple pipes.According to the fluorescence intensity that records, be calculated as follows histamine release rate (P release) and the histamine release suppression ratio (P inhibition) of respectively organizing cell respectively.
P discharges=(the spontaneous secretion histamine of supernatant histamine amount A-amount)/(histamine amount B in the supernatant histamine amount A+ cell) * 100%;
P inhibition=(1-medicine group histamine release amount/model group histamine release amount) * 100%.
Histamine amount in wherein spontaneous secretion histamine amount=normal cell supernatant.
Each organizes experimental data, and (variance analysis is used in ± S) expression, and each experimental group and matched group are compared, and there is the significance meaning P＜0.05 for difference, and there is the highly significant meaning P＜0.01 for difference with mean ± standard deviation.
Discharge model by antihistamine and record example 3 preparation Rhizoma Smilacis Glabrae extract histamine release dose-effect relationship such as tables 1:
Table 1-embodiment 3 Rhizoma Smilacis Glabrae extract histamine release rates
As shown in Table 1, have the effect that suppresses histamine release by the Rhizoma Smilacis Glabrae extract of embodiment 3 preparation when the concentration 1.6025ug/ml-25ug/ml, when 25ug/ml, the histamine release rate is 7.55%.
Effect embodiment 2 Rhizoma Smilacis Glabrae extracts suppress the histamine release experiment
Rhizoma Smilacis Glabrae extract is by embodiment 15 preparation, and except the medicine that adds has changed embodiment 15 preparations into, all the other determination steps are with effect embodiment 1.
Discharge model by antihistamine and record example 15 preparation Rhizoma Smilacis Glabrae extracts inhibition histamine release dose-effect relationship such as tables 2:
Table 2-embodiment 15 Rhizoma Smilacis Glabrae extract histamine release rates
As shown in Table 2, have the effect that suppresses histamine release by the Rhizoma Smilacis Glabrae extract of embodiment 15 preparation when the concentration 1.6025ug/ml-25ug/ml, when 25ug/ml, the histamine release rate is 6.84%.
Embodiment 17
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the water, the mass ratio of Rhizoma Smilacis Glabrae and water is 2/1, under 100 ℃, soak 0.1h, filter, filtrate concentrating obtained the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HPD100 absorption, adopting volumetric concentration is that 30% ethanol water is that eluant carries out eluting, is concentrated into extractum, obtains Rhizoma Smilacis Glabrae extract.
Embodiment 18
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the organic solvent ethanol, the alcoholic acid mass ratio of Rhizoma Smilacis Glabrae and organic solvent is 1/40, under 20 ℃, soak 72h, filter, filtrate concentrating obtained the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HZ891 absorption, adopting volumetric concentration is that 50% ethanol water is that eluant carries out eluting, is concentrated into extractum, obtains Rhizoma Smilacis Glabrae extract.
Embodiment 19
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the ethanol water, alcoholic acid volumetric concentration is 10%, the mass ratio of Rhizoma Smilacis Glabrae and ethanol water is 1/6, under 30 ℃, soaks 20h, filter, filtrate concentrated obtain the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin HZ891 absorption, and adopting volumetric concentration is that 60% methanol aqueous solution is that eluant carries out eluting, be concentrated into extractum, obtain Rhizoma Smilacis Glabrae extract.
Embodiment 20
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the methanol aqueous solution, the volumetric concentration of methanol is 95%, the mass ratio of Rhizoma Smilacis Glabrae and methanol aqueous solution is 1/8, under 90 ℃, soaks 60h, filter, filtrate concentrated obtain the solution that crude drug concentration is 1mg/ml, this solution is after non-polar resin D101 absorption, and adopting volumetric concentration is that 95% methanol aqueous solution is that eluant carries out eluting, be concentrated into extractum, obtain Rhizoma Smilacis Glabrae extract.
Embodiment 21
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the methanol aqueous solution, and the volumetric concentration of methanol is 85%, and the mass ratio of Rhizoma Smilacis Glabrae and methanol aqueous solution is 1/20, under 90 ℃, soaks 60h, filters, and obtains Rhizoma Smilacis Glabrae extract.
Embodiment 22
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the ethanol water, and alcoholic acid volumetric concentration is 70%, and the mass ratio of Rhizoma Smilacis Glabrae and ethanol water is 1/30, under 50 ℃, soaks 36h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 23
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 15MPa, 30 ℃ of extraction temperature, carbon dioxide flow 25kg/h carries out supercritical extraction, extraction time 4h, separating still is adjusted separating pressure 3MPa then, and separation temperature is 50 ℃, isolate fluid extract, get Rhizoma Smilacis Glabrae extract.
Embodiment 24
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the extraction still of carbon dioxide supercritical fluid extraction device, adopt the food-grade carbon-dioxide extracting pressure at 45MPa, 55 ℃ of extraction temperature, carbon dioxide flow 8kg/h carries out supercritical extraction, extraction time 0.5h, separating still is adjusted separating pressure 10MPa then, separation temperature is 30 ℃, isolate fluid extract, with the fluid extract volumetric concentration is that 75% ethanol is adjusted to the solution that crude drug concentration is 1mg/ml, and this solution is after non-polar resin HZ891 absorption, and adopting volumetric concentration is that 50% ethanol water is that eluant carries out eluting, be concentrated into extractum, obtain Rhizoma Smilacis Glabrae extract.
Embodiment 25
The natural plants Rhizoma Smilacis Glabrae is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, feeding pressure is the 0.12MPa water vapour, and distillation time is 4h, collects the liquid on the water layer in the condenser then, gets Rhizoma Smilacis Glabrae extract.
Embodiment 26
Described steam distillation is that the natural plants Rhizoma Smilacis Glabrae is pulverized the grate that is placed on the shop gauze, grate is put into the rustless steel steamer that connects condenser, and feeding pressure is the 0.15MPa water vapour, and distillation time is 0.5h, collect the liquid on the water layer in the condenser then, get Rhizoma Smilacis Glabrae extract.
Embodiment 27
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the ethanol water, and alcoholic acid volumetric concentration is 95%, and the mass ratio of Rhizoma Smilacis Glabrae and ethanol water is 1/30, under 70 ℃, extract 10h, filter, concentrate, dry, add 1% sulfuric acid solution precipitation, collecting precipitation, drying, get Rhizoma Smilacis Glabrae extract, wherein contain Taxifolin, Smiglanin, dimethyl succinic acid, syringic acid.
Embodiment 28
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the ethanol water, and alcoholic acid volumetric concentration is 95%, and the mass ratio of Rhizoma Smilacis Glabrae and ethanol water is 1/30, under 20 ℃, extract 336h, filter, be concentrated into extractum, admix 4 times of amount 80-100 order silica gel, use 30-60 ℃ of petroleum ether, chloroform, methanol extraction successively, collect chloroform eluting part, concentrate, drying gets Rhizoma Smilacis Glabrae extract.
Embodiment 29
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the glycerine water solution, and the volumetric concentration of glycerol is 10%, and the mass ratio of Rhizoma Smilacis Glabrae and glycerine water solution is 1/40, under 100 ℃, extracts 1h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 30
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the aqueous solution of propylene glycol, and the volumetric concentration of propylene glycol is 1%, and the mass ratio of Rhizoma Smilacis Glabrae and aqueous solution of propylene glycol is 1/20, under 95 ℃, extracts 2h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 31
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the 1,3 butylene glycol aqueous solution, and the volumetric concentration of 1,3 butylene glycol is 30%, and the mass ratio of Rhizoma Smilacis Glabrae and 1,3 butylene glycol aqueous solution is 1/10, under 90 ℃, extracts 3h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 32
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the dipropylene glycol aqueous solution, and the volumetric concentration of dipropylene glycol is 15%, and the mass ratio of Rhizoma Smilacis Glabrae and dipropylene glycol aqueous solution is 1/40, under 85 ℃, extracts 6h, filters, and concentrates, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 33
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the 1,3 butylene glycol, and the mass ratio of Rhizoma Smilacis Glabrae and 1,3 butylene glycol is 1/10, under 90 ℃, extracts 3h, filters, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 34
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the propylene glycol, and the mass ratio of Rhizoma Smilacis Glabrae and propylene glycol is 1/5, under 90 ℃, extracts 3h, filters, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 35
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the glycerol, and the mass ratio of Rhizoma Smilacis Glabrae and glycerol is 1/10, under 90 ℃, extracts 3h, filters, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 36
The pulverizing of natural plants Rhizoma Smilacis Glabrae is placed in the dipropylene glycol, and the mass ratio of Rhizoma Smilacis Glabrae and dipropylene glycol is 1/10, under 70 ℃, extracts 10h, filters, and gets Rhizoma Smilacis Glabrae extract.
Embodiment 37
The natural plants Rhizoma Smilacis Glabrae pulverized to be placed on dipropylene glycol, propylene glycol, glycerol, 1,3 butylene glycol volume ratio be 1: 1: 1: in 1 the mixed liquor, the mass ratio of Rhizoma Smilacis Glabrae and mixed liquor is 1/10, under 70 ℃, extracts 10h, filters, must Rhizoma Smilacis Glabrae extract.
Embodiment 38
The natural plants Rhizoma Smilacis Glabrae pulverized to be placed on dipropylene glycol, propylene glycol, glycerol, 1,3 butylene glycol, water volume ratio be 1: 1: 1: in 1: 1 the mixed liquor, the mass ratio of Rhizoma Smilacis Glabrae and mixed liquor is 1/10, under 70 ℃, extract 10h, filter, get Rhizoma Smilacis Glabrae extract.
Embodiment 39
The pulverizing of 100g natural plants Rhizoma Smilacis Glabrae is placed in the water, and the mass ratio of Rhizoma Smilacis Glabrae and water is 1/10, under 70 ℃, extracts 10h, filter, simmer down to extractum, it is in 70% the ethanol water that extractum is added the 1000g volumetric concentration, precipitation, filter, concentrate, get Rhizoma Smilacis Glabrae extract.
Embodiment 40
100g natural plants Rhizoma Smilacis Glabrae pulverized to be placed on volumetric concentration be that the mass ratio of Rhizoma Smilacis Glabrae and ethanol water is 1/10 in 70% the ethanol water, under 70 ℃, extraction 10h, filter, simmer down to extractum adds extractum in the 1000g water precipitation, filter, concentrate, get Rhizoma Smilacis Glabrae extract.
The extract that adopts the foregoing description method to obtain is applied to suppress in medicine, cosmetics or the health food of histamine release, thereby it is antipruritic to reach skin, anti-allergic effects, extract comprises purified Rhizoma Smilacis Glabrae extract or Rhizoma Smilacis Glabrae extract monomer after solvent extraction, carbon dioxide supercritical fluid extraction, the extraction, and the Rhizoma Smilacis Glabrae extract monomer comprises Taxifolin, Smiglanin, dimethyl succinic acid, syringic acid or the like.
Claims (8)
1. the application process of a natural plants Rhizoma Smilacis Glabrae extract, it is characterized in that, this application process is that the natural plants Rhizoma Smilacis Glabrae extract is applied to suppress in medicine, cosmetics and the health food of histamine release, antiallergic is provided, antipruritic effect, it is 0.001%～100% (W/W) that described Rhizoma Smilacis Glabrae extract adds concentration range.
2. the application process of a kind of natural plants Rhizoma Smilacis Glabrae extract according to claim 1, it is characterized in that, described natural plants Rhizoma Smilacis Glabrae extract comprises the thick Rhizoma Smilacis Glabrae extract that adopts solvent extraction, steam distillation or carbon dioxide supercritical fluid extraction to obtain, and the refining Rhizoma Smilacis Glabrae extract and the monomer whose that adopt process for purification to obtain after the extraction.
3. the application process of a kind of natural plant peony extract according to claim 2, it is characterized in that, described solvent extraction is the natural plants Rhizoma Smilacis Glabrae to be pulverized to be placed on extract in the solvent, Rhizoma Smilacis Glabrae is 2/1-1/40 with the mass ratio that extracts solvent, under 20-100 ℃, soak 0.1-336h, filter, concentrate, get Rhizoma Smilacis Glabrae extract.
4. the application process of a kind of natural plants Rhizoma Smilacis Glabrae extract according to claim 3 is characterized in that, described extraction solvent is water, organic solvent or aqueous solutions of organic solvent.
5. the application process of a kind of natural plants Rhizoma Smilacis Glabrae extract according to claim 4 is characterized in that, described organic solvent is one or more in alcohol, ethyl acetate, dipropylene glycol or the petroleum ether of 1-5 carbon.
6. the application process of a kind of natural plants Rhizoma Smilacis Glabrae extract according to claim 4 is characterized in that, organic solvent is the alcohol of 1-5 carbon or in the dipropylene glycol one or more in the described aqueous solutions of organic solvent.
7. according to the application process of claim 5 or 6 described a kind of natural plants Rhizoma Smilacis Glabrae extracts, it is characterized in that the alcohol of a described 1-5 carbon comprises ethanol, methanol, glycerol, propylene glycol, 1,3 butylene glycol.
8. the application process of a kind of natural plants Rhizoma Smilacis Glabrae extract according to claim 1 is characterized in that, described medicine, cosmetics and health food are respectively the receivable form of medicine, cosmetics and health food.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104220084A (en) * | 2012-03-30 | 2014-12-17 | 延世大学校产学协力团 | Pharmaceutical compositions comprising an extract from smilax china linne for preventing or treating obesity, hyperlipidemia or fatty liver |
| CN106821859A (en) * | 2017-03-16 | 2017-06-13 | 上海芝映生物科技有限公司 | A kind of preparation method of the composite plant extract liquid with antiallergic itching-relieving action |
| CN110613667A (en) * | 2019-10-29 | 2019-12-27 | 广州市博之越精细化工有限公司 | Formula and application of allergy-relieving repairing essence |
-
2009
- 2009-07-31 CN CN200910055790A patent/CN101612292A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104220084A (en) * | 2012-03-30 | 2014-12-17 | 延世大学校产学协力团 | Pharmaceutical compositions comprising an extract from smilax china linne for preventing or treating obesity, hyperlipidemia or fatty liver |
| CN104220084B (en) * | 2012-03-30 | 2018-10-09 | 延世大学校产学协力团 | Include prevention or treatment pharmaceutical composition of the Rhizoma Smilacis Glabrae extract as the obesity of active ingredient, hyperlipidemia or fatty liver |
| CN106821859A (en) * | 2017-03-16 | 2017-06-13 | 上海芝映生物科技有限公司 | A kind of preparation method of the composite plant extract liquid with antiallergic itching-relieving action |
| CN106821859B (en) * | 2017-03-16 | 2022-06-17 | 上海芝映生物科技有限公司 | Preparation method of composite plant extract with anti-allergy and itching-relieving effects |
| CN110613667A (en) * | 2019-10-29 | 2019-12-27 | 广州市博之越精细化工有限公司 | Formula and application of allergy-relieving repairing essence |
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Open date: 20091230 |