Background technology
Fatigue is a kind of natural phenomena, because work, learning tasks are heavy, due to the rhythm of life anxiety.Fatigue comprises physiology and psychological two aspects.Physiological fatigue mainly shows as DOMS, general fatigue etc.; And mental fatigue mainly shows as mood agitation, absent minded, thought slowness etc.20th century the mid-80, medical circle has proposed " chronic fatigue syndrome " this notion.The chronic fatigue syndrome main clinical manifestation has: with corporality fatigue is main, often with headache, sore-throat, muscle and arthralgia, and decrease of memory, low-heat, depressed etc.
The easiest crowd who is in fatigue state is following several kinds: the one, for a long time in the face of keen competition pressure, the huge crowd of psychological burden, like the operator of enterprises and institutions, leader, private owner etc.; The 2nd, strong, the busy brain worker of dedication like scientific research personnel, journalist, government official etc., concentrates to be " white collar " and " golden collar personage "; The 3rd, long-term excess load, the in tension physical labourer of spirit are like the workman in the labor-intensive enterprises, taxi driver etc.Though many people can work on, ability to work and efficient obviously descend, and fatigue symptom is not alleviated because of rest.Some special population is exactly the sportsman who is in over a long time under the high pressure training, and they participate in training, match for a long time; Thought; Spirit is in high-strung stress situation, is easy to produce fatigue, if in time do not eliminate tired; Can cause lifelong injury, this also is that a lot of outstanding sportsmen of China shift to an earlier date retired one of the main reasons.
Goal of the invention
The object of the invention just provides a kind of health food preparation, and this health food preparation can improve the anoxia endurance of human body again can alleviating physical fatigue.
Another object of the present invention just provides the preparation method of a kind of anoxia endurance that had not only improved human body but also health food preparation that can alleviating physical fatigue.
Its active ingredient of health food preparation of alleviating fatigue of the present invention is processed by the following materials based on weight medicine:
Rhodiola root 500-700 part, gynostemma pentaphylla 500--700 part, fruit of Chinese wolfberry 250-350 part, American Ginseng 170--230 part.
Preferred weight proportion is: rhodiola root 540--660 part, gynostemma pentaphylla 540--660 part, fruit of Chinese wolfberry 270-330 part, American Ginseng 180--220 part.
The optimum weight proportioning is: 600 parts of rhodiola roots, 600 parts of gynostemma pentaphylla, 300 parts of the fruits of Chinese wolfberry, 200 parts of American Ginsengs.
This product is guidance with the traditional Chinese medical theory, in conjunction with the modern scientific research achievement.Hold the tired mechanism that produces on the whole, take the formulation principle of " tonifying speen and tonifying kidney, supplementing qi and nourishing yin ".Reach the effect that improves anoxia endurance and alleviating physical fatigue function with Chinese medicine compound prescription.
Wherein, said rhodiola root is monarch in we.Its flavor is sweet can be mended, can strengthening the spleen and replenishing qi, kindly control deficiency-weakness of spleen-QI, and lassitude hypodynamia again can tonifying lung gas, supports lung yin, controls deficiency of both vital energy and Yin, and cough with ropy sputum is single with certain curative effect is promptly arranged; Put down in writing rhodiola root " cool in nature, heat-clearing, nourishing vigour " in Tibetan medicine's Four-Volume Medical Code, indicating rhodiola root in " Compendium of Materia Medica grass portion " the 20th volume is " draft is top grade ", and tool is strengthened the body resistance to consolidate the constitution, effects such as benefiting qi and nourishing blood.It is sweet can the benefit, can strengthening the spleen and replenishing qi, kindly control deficiency-weakness of spleen-QI, and lassitude hypodynamia again can tonifying lung gas, supports lung yin.
This product can strengthen sportsman's physique at the volley and improve locomitivity, and tired elimination is accelerated in the generation of delay fatigue, does not contain forbidden amphetamine and toxic and side effect again.Being suitable for the sportsman uses.
Theoretical according to modern medicine and Human physiology; Gadol extract (mainly containing rhodioside) all is significantly increased to animal normal pressure hypoxia-bearing capability and locomitivity; Can quicken lactic acid metabolism, reduce MCO, reach antifatigue effect thereby improve cardiac reserve; Gypenosides (Gys) has resistance to exhaust the booster action of motion back free radical, and can make SOD, and the activity of GSP-Px improves, and makes it meet or exceed normal level, thereby the protection eucaryotic cell structure exempts from destruction, improves erythrocytic fortune oxygen function; The thick polysaccharide of American ginseng root has the effect of remarkable antagonism caused by cyclophosphamide murine interleukin and immune organ weight minimizing.Phagocytic function to normal and immunocompromised mouse reticuloendothelial system has tangible activation.Can promote simultaneously the conversion of immunologic hypofunction mouse spleen lymphocyte.The thick polysaccharide of prompting American ginseng root has humidification to the nospecific immunity and the cellular immune function of body; LBP-X can increase mouse muscle glycogen, glycogen significantly.Therefore, these article can significantly improve the anoxia endurance and the alleviating physical fatigue of human body through rational formula and interaction.
Explain from the research of modern medicine angle: rhodiola root can increase administration group mouse erythrocyte (Hb) content, and Hb becomes to help the transportation of oxygen more, and mouse movement endurance is strengthened.Regulate nervous centralis.Gadol extract (mainly containing rhodioside) all is significantly increased to animal normal pressure hypoxia-bearing capability and locomitivity, can quicken lactic acid metabolism, reduces MCO, reaches antifatigue effect thereby improve cardiac reserve; Rhodiola root can also reduce oxygen consumption speed in addition, can strengthen the oxygen pressure reduction of venous blood again, increases and supports, and improves the endurance of animal to anoxic.
We are minister with the fruit of Chinese wolfberry, gynostemma pentaphylla.The fruit of Chinese wolfberry sweet property of distinguishing the flavor of is flat, and merit specially nourishes, effect such as moistening lung, clearing liver, beneficial gas, production of sperm, supporing yang, qi-restoratives labor are arranged, dispel the wind, make eye bright; In traditional Chinese medical science works, have in a large number and have " record of feeling at ease to allay excitement " about matrimony vine; " property of medicine opinion " carried its " not beneficial essence, various symptims and signs of deficiency ", the Shennong's Herbal record: perverse trend in the matrimony vine master five, to pine for quenching one's thirst, and general arthralgia rheumatism is obeyed hard muscles and bones for a long time, and it is not old to make light of one's life by commiting suicide, cold-resistant heat." dietetic materia medica " record: the hard muscle of matrimony vine is anti-old, dispels the wind, and help muscles and bones, the beneficial people of ability goes empty old.The essentials of Matea Medica record: the clearing liver of matrimony vine profit liver, nourshing kidney benefit gas, production of sperm is supporing yang, qi-restoratives labor, strengthening the bones and muscles, dispelling pathogenic wind for improving eyesight, sharp stool and urine.Control that choke is dried quenches one's thirst.
Modern medicine study shows that the fruit of Chinese wolfberry not only contains rich nutrient contents, but also contains multiple pharmacological component, has high medical value, is widely used clinically.Such as liver kidney two warps, have nourishing liver and kidneyly, the effect of benefiting essence-blood is the good merchantable brand of flat filling liver kidney.There is experiment to observe fruit of Chinese wolfberry decocting liquid to mouse cold resistant, anti-anoxic and the fatigue proof influence of swimming.The result shows that matrimony vine decocting liquid can improve the cold resistant ability of mouse under low temperature environment, prolongs small white mouse swimming time at room temperature simultaneously, improves anti-fatigue ability, and prolongs the hypoxia endurance time of mouse under normal pressure.Explain that the fruit of Chinese wolfberry can eliminate exercise induced fatigue, also can build up health.
Gynostemma pentaphylla is a cucurbitaceous plant, has another name called seven leaf courages, is distributed widely in semi-tropical herbaceous perennial vine plant.Its root or stem all herbal medicine, pharmacology and health-care effect are extensive, give birth to cold, nontoxic.Can go into spleen and replenish qi to invigorate the spleen, return the heat-clearing of lung benefit gas again, can assist a ruler in governing a country the rhodiola root alleviating physical fatigue, improve the effect of anoxia endurance, its main active ingredient is gypenosides (Gys).
The modern study document confirms: gypenosides has resistance to exhaust the booster action of motion back free radical; And can make SOD, the Red Star of GSP-Px improves, and makes it meet or exceed normal level; Thereby the protection eucaryotic cell structure exempts from destruction, improves erythrocytic fortune oxygen function; Gys pours into to mouse, can obviously prolong the time that mouse is swum and exhausts to power, has the raising locomitivity, antifatigue, anti-heart kidney radical damage.
We are assistant with the American Ginseng.American Ginseng is cool in nature and mend, and " new compilation of materia medica " sayed its " fire falls in tonifying lung, and born fluid goes out to be tired of tired, empty and have the fiery person suitable ", and the function boosting qi and nourishing yin relieves inflammation or internal heat and promotes the production of body fluid, the kind card of controlling deficiency of both qi and yin.
See that from the physiology of exercise angle before fatigue state occurred, the various biochemical indicators of body changed.Exercise tolerance be the reflection organism fatigue the most directly, index the most objectively, the American Ginseng sheet can prolong the swimming time of mouse.And in the tired biochemical indicator of reaction, urea nitrogen, blood lactic acid, hepatic glycogen are the most representative.The catabolism of protein and nitrogen-containing compound makes urea content increase in the body, and body is reduced the adaptive faculty of exercise load ability.The urea nitrogen of mouse does not increase in the test has conspicuousness ground to descend on the contrary.The protein depletion amount of mouse does not significantly increase in the account for motion process.Body mainly relies on the anaerobic glycolysis energize when being in the height anaerobic condition after because of a large amount of motions.Lactic acid is the product of sugared anerobic glycolysis, and long-time strenuous exercise makes lactic acid accumulation in the body too much can influence the interior eubolism process of relatively stable and body of internal milieu.The blood lactate level can reflect the generation and the elimination speed of aerobic capacity, fatigue.The blood lactic acid TG-AUC of mouse obviously reduces after swimming with a load attached to the body, explains that blood lactic acid amplitude of variation is very little, explains that also the fatigue of mouse increases slowly, eliminates soon.The antifatigue effect of American Ginseng sheet also has been described on the mechanism.The American Ginseng sheet quickens the clearance rate of urea nitrogen in the mouse body in the research; Improved the swimming time of mouse; Reduced blood lactic acid TG-AUC.These result of the tests have reflected that directly the American Ginseng sheet has certain antifatigue effect.
In raising anoxia endurance of the present invention and the alleviating physical fatigue health care foods preparation, rhodiola root is sweet can the benefit, can strengthening the spleen and replenishing qi, and the kind deficiency-weakness of spleen-QI of controlling; Lassitude hypodynamia again can tonifying lung gas, supports lung yin; Control deficiency of both vital energy and Yin, cough with ropy sputum, single with certain curative effect is promptly arranged.American Ginseng is cool in nature and mend, and " new compilation of materia medica " sayed its " fire falls in tonifying lung, and born fluid goes out to be tired of tired, empty and have the fiery person suitable ", and the function boosting qi and nourishing yin relieves inflammation or internal heat and promotes the production of body fluid, the kind card of controlling deficiency of both qi and yin.The fruit of Chinese wolfberry sweet property of distinguishing the flavor of is flat, and merit specially nourishes, effect such as moistening lung, clearing liver, beneficial gas, production of sperm, supporing yang, qi-restoratives labor are arranged, dispel the wind, make eye bright.Zhu Pin is general, plays tonifying speen and tonifying kidney altogether, the merit of supplementing qi and nourishing yin.Thereby reach the effect that improves anoxia endurance and alleviating physical fatigue function.Efficient is 81.67%.
Health-care preparation with raising anoxia endurance and two health cares of alleviating physical fatigue of the present invention is an oral formulations, preferred particulates, tablet or capsule.
Preparation method with the health food preparation that improves anoxia endurance and two health cares of alleviating physical fatigue of the present invention; Comprise ginseng powder is broken into fine powder; With rhodiola root, gynostemma pentaphylla etc. two flavor medicines through the 60-80% alcohol extract, with the fruit of Chinese wolfberry through decocting, with the ethanol extract of rhodiola root and gynostemma pentaphylla and the fruit of Chinese wolfberry aqueous solution respectively through concentrate, after drying and the pulverizing and fine powders such as American Ginseng and dextrin mix; Process particle again, or again with particle compressing tablet or can capsule.
Preferably, take by weighing rhodiola root and gynostemma pentaphylla, place in the extractor, add 70% ethanol, reflux 2 times by said weight portion.Add for the first time 8 times of amount 70% ethanol, extracted 2 hours; Add for the second time 6 times of amount 70% ethanol, extracted 1 hour.Extract filters, and merges filtrating twice, places in the vacuum concentration pan, and decompression recycling ethanol, and be concentrated into 50 ℃ when surveying relative densities and being 1.10~1.20g/ml is transported in the vacuum concentration pot by pipeline, and being concentrated into 50 ℃, to survey relative densities be 1.20~1.30g/ml.
Take by weighing the fruit of Chinese wolfberry by said weight portion, add appropriate amount of purified water, decoct 2 times; For the first time add 10 times of water gagings, decocted 2 hours, decoction liquor filters to be derived; For the second time, filter residue is added 8 times of water gagings once more decocted 1 hour, decoction liquor filters; Merge filtrating twice, being concentrated into 50 ℃ of survey relative densities is 1.20~1.30g/ml.
Rhodiola root, gynostemma pentaphylla alcohol extracting concentrate and the vacuum drying of fruit of Chinese wolfberry water extracting liquid, vacuum-0.08MPA, dry respectively under the condition of temperature below 80 ℃, get dry extract, moisture<5%.
After dry extract is pulverized respectively, cross 80 mesh sieves, get dry extract.
Take by weighing ginseng powder, dextrin by said weight portion, do with two kinds of dry extracts and mix, add inlet from material and slowly add 95% ethanol, wet mixing is granulated; Oven dry makes moisture≤6%; Whole grain.
Said American Ginseng is the fines medicine, and price is higher, takes on the technology to pulverize the way that filled capsules is directly mixed in the back, to avoid its loss as far as possible more.Because its active ingredient is ginsenoside, and pulverizing is thinner, can guarantee its human absorptivity equally.Be crushed to 80 order fine powders.Breaking method is a mechanical disintegration.
Preparation method with the health food preparation that improves anoxia endurance and two health cares of alleviating physical fatigue of the present invention is scientific and reasonable, has fully kept the effective elements of the medicine of respectively distinguishing the flavor of, thereby has guaranteed the validity of health food preparation.
The specific embodiment
Further set forth the preparation method of health food of the present invention below through embodiment.
Embodiment 1
A kind of health food preparation with raising anoxia endurance and two health cares of alleviating physical fatigue, it is processed by the bulk drug and the auxiliary material of following parts by weight:
Rhodiola root: 600g, gynostemma pentaphylla: 600g, the fruit of Chinese wolfberry: 300g,
American Ginseng: 200g, dextrin: 100g.
During preparation, earlier ginseng powder is broken into fine powder; Reclaim ethanol after rhodiola root, gynostemma pentaphylla used 70% alcohol extract 2 times, and simmer down to alcohol extracting concentrate; After fruit of Chinese wolfberry water carried 2 times, the simmer down to water extracting liquid.The dry respectively dry extract that gets is ground into fine powder, mixes with American Ginseng fine powder, dextrin, processes particle and dry, and the can capsule gets final product.
Wherein, said American Ginseng before preparation with irradiation sterilization.Radiation parameter: 1. irradiation dose 4.0KGY; 2. exposure time is 2 hours; 3. unevenness≤2.5; 4. environmental condition: 25 ℃ of temperature, relative humidity 60%. Said rhodiola root, gynostemma pentaphylla are behind check character standardization separately, and impurity is removed in selection respectively, and clear water is cleaned, and dries subsequent use; Behind the said fruit of Chinese wolfberry check character standardization, impurity is removed in selection, and is subsequent use.In production process, rhodiola root, gynostemma pentaphylla, the fruit of Chinese wolfberry all pass through the high temperature extraction process, and corresponding sterilization function is arranged; Dextrin can use through behind the hygiene inspection.Therefore, can guarantee that the hygiene of these article is qualified.
Said pure extracting method is: take by weighing rhodiola root, gynostemma pentaphylla by said weight portion, place in the multi-function extractor, add 70% ethanol, reflux 2 times.Add for the first time 8 times of amount 70% ethanol, extracted 2 hours; Add for the second time 6 times of amount 70% ethanol, extracted 1 hour.Extract filters, and merges filtrating twice, places in the vacuum concentration pan; Decompression recycling ethanol; And to be concentrated into relative density be about D=1.10~1.20g/ml when (50 ℃), is transported in the vacuum concentration pot by pipeline, is concentrated into relative density and is about D=1.20~1.30g/ml (50 ℃) time to emit.
Said water extracting method is: take by weighing the fruit of Chinese wolfberry by said weight portion, add appropriate amount of purified water, decoct 2 times.For the first time add 10 times of water gagings, decocted 2 hours, decoction liquor filters to be derived; For the second time, filter residue is added 8 times of water gagings once more decocted 1 hour, decoction liquor filters.Merge filtrating twice, be transported in the vacuum concentration pot, be concentrated into relative density and be about D=1.20~1.30g/ml (50 ℃) time to emit by pipeline.
Wherein, Described mixing filtrating and water extract concentration process are at vacuum concentration pan (ZZNG; Ten thousand logical pharmaceutical equipment Co., Ltds produce by Jilin) and middle completion of vacuum concentration pot (300, produce by Wenzhou City Machinery Plant of Light Industry of Shenhua), vacuum degree control is at 0.07Mpa; So that the liquid temperature per hour can be evaporated moisture content 60-80 liter 50--70 ℃ of evaporation down.
Said drying and crushing process is: rhodiola root, gynostemma pentaphylla alcohol extracting concentrate and fruit of Chinese wolfberry water extracting liquid are packed vacuum drying into in the stainless steel disc, place difference drying in the vacuum drying chamber, get dry extract.Dry extract is pulverized, and sieves.
Wherein, the dry run of said each concentrate is: use vacuum drying chamber (GGX-22 is produced by Jilin Province ten thousand logical pharmaceutical equipment Co., Ltds), at vacuum-0.08MPA, 75 ℃ of temperature reach moisture<5%, become dry extract, and paste-forming rate is 15%; The said dry extract granularity of sieving is 80 orders.
Said processes of mixing and granulating is: take by weighing ginseng powder, dextrin by said weight portion, put into the efficient wet mixer-granulator with two kinds of dry extracts and do to mix, add inlet from material simultaneously and slowly add ethanol and process softwood as wetting agent, the granulation of sieving; Put the heated-air circulation oven oven dry, get dried particles, carry out whole grain with waving granulator again.Whole good particle is capsule 's content.
The quality-controlling parameters of this particle is:
1, moisture: 3-6%, usually<6%
2, granularity: 14-22 order
3, total saponin(e and thick polyoses content: in every 100g particle, contain total saponin(e (in the ginsenoside Re) and must not be less than 2.0g; In every 100g particle, contain thick polysaccharide (in glucan) and must not be less than 1.2g.
Wherein, dried the doing time of said efficient wet mixer-granulator is 5 minutes; Said concentration of ethanol is 95%; The said softwood granularity of sieving is 16 orders; Said heated-air circulation oven temperature is 80 ℃; Be 2.0 hours drying time, moisture<6%.
Said can capsule process is: through dried particle after the assay was approved, fill with automatic capsule filling machine, capsule is No. 0, and the adjustment loading amount is every 0.50g, and ERROR CONTROL is ± 5.0%.Populated capsule carries out polishing with the capsule polishing machine to capsule.
Embodiment 2:
The health food preparation of the described alleviating fatigue of present embodiment, it is processed by the bulk drug and the auxiliary material of following parts by weight:
Rhodiola root: 540g, gynostemma pentaphylla: 660g, the fruit of Chinese wolfberry: 270g, American Ginseng: 220g, dextrin: 100g.
Press embodiment 1 said preparation method, be prepared into capsule.
Embodiment 3:
The health food preparation of the described alleviating fatigue of present embodiment, it is processed by the bulk drug and the auxiliary material of following parts by weight:
Rhodiola root: 660 parts, gynostemma pentaphylla: 540 parts, the fruit of Chinese wolfberry: 330 parts, American Ginseng: 180 parts, dextrin: 100g.
During preparation, earlier ginseng powder is broken into fine powder; Reclaim ethanol after rhodiola root, gynostemma pentaphylla used 70% alcohol extract 2 times, and simmer down to alcohol extracting concentrate; After fruit of Chinese wolfberry water carried 2 times, the simmer down to water extracting liquid.The dry respectively dry extract that gets is ground into fine powder, mixes with American Ginseng fine powder, dextrin, processes particle and dry, promptly can be made into granule.
Embodiment 4:
The health food preparation of the described alleviating fatigue of present embodiment, it is processed by the bulk drug and the auxiliary material of following parts by weight:
Rhodiola root: 500 parts, gynostemma pentaphylla: 700 parts, the fruit of Chinese wolfberry: 350 parts, American Ginseng: 230 parts, dextrin: 100g.
Press embodiment 1 said preparation method, be prepared into capsule.
Embodiment 5:
The health food preparation of the described alleviating fatigue of present embodiment, it is processed by the bulk drug and the auxiliary material of following parts by weight:
Rhodiola root: 700 parts, gynostemma pentaphylla: 500 parts, the fruit of Chinese wolfberry: 250 parts, American Ginseng: 170 parts, dextrin: 100g.
During preparation, earlier ginseng powder is broken into fine powder; Reclaim ethanol after rhodiola root, gynostemma pentaphylla used 70% alcohol extract 2 times, and simmer down to alcohol extracting concentrate; After fruit of Chinese wolfberry water carried 2 times, the simmer down to water extracting liquid.The dry respectively dry extract that gets is ground into fine powder, mixes with American Ginseng fine powder, dextrin and processes particle, again the particle compressing tablet promptly can be made into tablet.
Below come further to set forth the beneficial effect of health products according to the invention through Test Example.Test Example 1: alleviating physical fatigue function test
1.2 animal used as test: the ICR cleaning level male mice of selecting for use preclinical medicine institute of Jilin University zoopery center to provide, body weight 18g-22g, production licence number: SCXK-(Ji) 2003-0001.The mouse basal feed provides production licence number by the living animal used as test feed factory of Changchun Lvyuan District benefit: SCXK-(Ji) 2003-0004.This cleaning level animal housing environmental facility quality certification number: lucky moving establishing word 10-1005 number, animal used as test occupancy permit number: SYXK (Ji) 2005-0009 is the cleaning level.
1.3 dosage is selected: the health-care edible capsule of alleviating fatigue among the said embodiment 1.Recommend the adult to take in 2 times (body weight is in 60kg) every day, each 3, the 0.5g/ grain; Be 50mg/kgBW d, irritate stomach dosage to recommend 1,10 and 30 times of adult's intake to be provided with day, promptly 50,500,1500mg/kg BWd, each dose groups is with distilled water diluting, and distilled water is made negative control group, and this sample need not be provided with sugared control group.Each is organized the mouse stomach amount and is 0.2mL/10g BW d, continuous irrigation stomach 30 days.
1.4 instrument and reagent: the swimming case (50cm * 50cm * 40cm), electronic balance, sheet lead, 722 spectrophotometers, stopwatch, blood lactic acid test enzyme membrane; Urea is measured kit; The hepatic glycogen kit steps auspicious BS-300 type automatic clinical chemistry analyzer, SBA-40C type bio-sensing analyzer etc.
1.5 experimental technique:
1.5.1 swimming with a load attached to the body test
After last is tried thing 30min, put mouse and swim in the case, the about 30cm of the depth of water, water temperature (25 native 1) ℃, the load sheet lead of 5% body weight of mouse root of the tail portion in swimming.The record mouse is from the extremely dead time of swimming beginning, as mouse swimming time (min).
1.5.2 serum urea is measured
Last is tried thing 30min, the 90min that in the water of temperature, swims for (30 ± 0.5) ℃, and rest 60min plucks the eyeball blood sampling, gets serum, carries out urea content and measures (kit: Shanghai Kehua Dongling Diagnosis Products Co., Ltd. provides).The advanced in years auspicious BS-300 type automatic clinical chemistry analyzer that adopts Mairui Biological Medical Electronic Co., Ltd., Shenzhen to produce is measured.
1.5.3 hepatic glycogen is measured:
Last is tried thing 30min execution mouse and is got liver, after the physiological saline rinsing, blots with filter paper, accurately takes by weighing liver 75mg; Every pipe adds the aqueous slkali that 225 μ L kits provide, and seals the mouth of pipe with film, pricks an air-vent; Boiling water bath 20min, the cooling back adds 7.2mL distilled water, fully mixing; Press the operation of reagent specification and add developer, (kit: Nanjing is built up bio-engineering research and provided), boiling water bath 5min; Carry out colorimetric estimation with 722 spectrophotometers at the 620nm wavelength, the formula conversion hepatic glycogen that by specification provides.
1.5.4 blood lactic acid is measured
Last is tried blood sampling 20 μ L behind the thing 30min; Be not swimming with a load attached to the body 10min in the water of (30 soil 0.5) ℃ again in temperature; Respectively at (swimming back 0min) and swimming back rest 20min 20 μ L that respectively take a blood sample immediately after the swimming, adopt the blood lactic acid value of three time points more than the bio-sensing analysis-e/or determining that the Shandong Province academy sciences Biology Research Institute produces.(hemolytic agent and mensuration liquid provide by this institute).With three time point blood lactic acid TG-AUCs (computing formula is seen after) judged result.
1.6 experimental data is added up with the variance analysis of spss10.0 software.
2 results
2.1 said health-care edible capsule is to the influence of mouse swimming with a load attached to the body time and mouse weightening finish
The said health-care edible capsule of table 1. is to the influence of mouse swimming with a load attached to the body time and mouse weightening finish
P: each experimental group and negative control group are relatively. *: compare P<0.05 with negative control group
Visible by table 1; Per os gives the said health-care edible capsule 30 days of mouse various dose; Each experimental mice weightening finish is compared there was no significant difference p>0.05 with negative control group; The high dose group swimming time is longer than negative control group, and difference has conspicuousness (P<0.05), explains that said health-care edible capsule can prolong the mouse swimming with a load attached to the body time and the mouse weightening finish is not had influence.
2.2 said health-care edible capsule is to the influence of mice serum urea and mouse weightening finish
The said health-care edible capsule of table 2. is to the influence of mouse swimming back serum urea and mouse weightening finish
P: each experimental group and negative control group be * relatively: compare p<0.05 with negative control group
Visible by table 2; Per os gives the said health-care edible capsule 30 days of mouse various dose; Each experimental mice weightening finish is compared there was no significant difference (P>0.05) with negative control group; High dose group mouse swimming back serum urea is lower than negative control group, and with negative control group significant difference (P<0.05) is arranged relatively, explains that said health-care edible capsule has to suppress effect that mouse swimming back serum urea raises and the mouse weightening finish is not had influence.
2.3 the health-care edible capsule of said alleviating fatigue is to the influence of Mouse Liver glycogen content and mouse weightening finish
The said health-care edible capsule of table 3. is to the influence of Mouse Liver glycogen content and mouse weightening finish
Extension rate (100 * 10) * 1.11 p before hepatic glycogen=(measuring pipe OD value)/(standard pipe OD value) * 0.01 (titer concentration of glucose) * mensuration: each experimental group and negative control group are relatively
The said health-care edible capsule visible by table 3, that per os gives the mouse various dose 30 days; Each experimental mice weightening finish is compared there was no significant difference (P>0.05) with negative control group; Each dose groups hepatic glycogen content is compared there was no significant difference (P>0.05) with control group, explain that the health-care edible capsule of said alleviating fatigue is not seen influence to hepatic glycogen deposit and mouse weightening finish.
2.4 said health-care edible capsule is to the influence of mouse blood lactic acid value and mouse body weight
Table 4. gives said health-care edible capsule mouse swimming forward and backward and three time point blood of rest 20min lactic acid value
The health-care edible capsule of the said alleviating fatigue of table 5. increases virgin influence to three time point blood of mouse lactic acid TG-AUC and mouse
Three time point blood lactic acid TG-AUC=5 * (blood lactic acid values 10 before the swimming * back 0 minute blood lactic acid value+2 * 20 fens blood lactic acid value of swimming back rest of swimming)
P: each experimental group and negative control group be * relatively: compare P<0.05 with negative control group
Visible by table 5; Per os gives the said health-care edible capsule 30 days of mouse various dose; Each experimental mice weightening finish is compared there was no significant difference (P>0.05) with negative control group; Swim back three time point blood lactic acid TG-AUCs and negative control group comparing difference of high dose group mouse has conspicuousness (P<0.05), the generation of blood lactic acid when said health-care edible capsule can reduce swimming is described and the mouse weightening finish is not had influence.
3, brief summary
Per os gives the said health-care edible capsule of mouse 50,500,1500mg/kg BW d; Continuous irrigation stomach 30 days; Can prolong the mouse swimming with a load attached to the body time with negative control group comparison 1500mg/kg BW d dose groups; The generation of serum urea when reducing mouse swimming, the generation of blood lactic acid when reducing swimming, each dose groups is not all seen influence to hepatic glycogen deposit and mouse body weight.Judge that thus said health-care edible capsule has the alleviating physical fatigue function.
Test Example 2: improve the anoxia tolerant function test
1, material and method
1.1 health-care edible capsule described in sample: the embodiment 1, content is chocolate brown powder capsule dress.Pulverize subsequent use with miniature Universalpulverizer.
1.2 animal used as test: the cleaning level ICR male mice that preclinical medicine institute of Jilin University zoopery center provides, production licence number: SCXK-(Ji) 2003-0001.Feed provides production licence number by the living animal used as test feed factory of Changchun Lvyuan District benefit: SCXK-(Ji) 2003-0004.This animal used as test environmental facility quality certification, the lucky moving word 10-1005 that establishes, animal used as test occupancy permit number: SYXK-(Ji) 2005-0009.
1.3 dosage is selected: 6/day of recommended amounts, the 0.5g/ grain is 3.0g/60kgBW, is equivalent to 0.05g/kgBW, irritates stomach dosage to recommend 1,10 and 30 times of adult's intake to be provided with day, promptly 0.05,0.50,1.50g/kgBW, each dose groups is with distilled water diluting.Make negative control group with distilled water, each is organized the mouse stomach amount and is 0.2mL/10gBW, and the continuous irrigation stomach is surveyed each item index after 30 days.
1.4 sample preparation: sample is ground into last use with miniature Universalpulverizer.Sample thief powder 3.75g adding distil water to the 50rnL constant volume that is mixed is high dose group and is tried thing; Sample thief powder 1.25g adding distil water to 50mL be mixed constant volume be in dose groups tried thing; Sample thief powder 0.125g adding distil water to the 50mL constant volume that is mixed is low dose group and is tried thing.Each group is tried the thing fresh, and irritate stomach and tried thing once at 9 o'clock in morning every day.
1.5 instrument and reagent: 250mL ground wide-mouth bottle, scissors, stopwatch, 1mL syringe, vaseline, soda lime, natrium nitrosum.
1.6 experimental technique:
1.6.1 normal pressure hypoxia endurance test
Per os gives each dose groups continuously and was tried thing 30 days, and control group gives equal capacity distilled water, after last is tried thing 1h; Each group mouse is put into the wide-mouth bottle (1/bottle) that fills the 5g soda lime respectively; Smear bottleneck with vaseline, cover completely, immediately timing; With the breath stopped is index, observes mouse because of the dead time of anoxic.
1.6.2 natrium nitrosum poisoning survival test
Per os gives each dose groups continuously and was tried thing 30 days; Control group gives equal capacity distilled water; After last was tried thing 1h, each organized the equal lumbar injection sodium nitrite solution of mouse 240mg/kg BW (0.1mL/10g), timing immediately; With the breath stopped is index, observes mouse because of the dead time of anoxic.
1.6.3 acute cerebral ischemia property hypoxia test
Per os gives each dose groups continuously and was tried thing 30 days; Control group gives equal capacity distilled water; After last is tried thing 1h, each treated animal (under the ether light anaesthesia) from neck by broken end only, immediately by behind the stopwatch record mouse broken end to the dwell time of breathing of dehiscing.
1.7 experimental data is carried out variance analysis with spss11.0 software.
2, result
2.1 health-care edible capsule according to the invention is to the influence of mouse normal pressure hypoxia endurance test and mouse weightening finish
Table 1. health-care edible capsule according to the invention is to the influence of mouse normal pressure hypoxia endurance test and mouse weightening finish
P: each experimental group and negative control group be p*<0.05 relatively
Visible by table 1; Per os gives health-care edible capsule according to the invention 30 days; Each is organized the mouse weightening finish and compares there was no significant difference (p>0.05) with negative control group; Low, middle dose groups mouse normobaric hypoxia time-to-live and negative control group relatively do not have significant difference (p>0.05); High dose group mouse normobaric hypoxia time-to-live and negative control group relatively have significant difference (p<0.05), explain that health-care edible capsule according to the invention can prolong the mouse normobaric hypoxia time and the mouse weightening finish is not had influence.
2.2 said health-care edible capsule is to the influence of mouse natrium nitrosum poisoning survival test and mouse weightening finish
The said health-care edible capsule of table 2. is to the influence of mouse natrium nitrosum poisoning survival test and mouse weightening finish
P: each experimental group and negative control group compare: * p<0.05 * * p<0.01
Visible by table 2; Per os gives the said health-care edible capsule 30 days of mouse various dose; Each is organized the mouse weightening finish and compares there was no significant difference (p>0.05) with negative control group; Low dose group mouse natrium nitrosum poisoning anoxic time-to-live and negative control group be there was no significant difference (p>0.05) relatively; Middle dose groups mouse natrium nitrosum poisoning anoxic time-to-live and negative control group relatively have significant difference (p<0.05); High dose mouse natrium nitrosum poisoning anoxic time-to-live and negative control group relatively have highly significant property difference (p<0.01), explain that the said health-care edible capsule of middle and high dosage has the effect that prolongs the mouse natrium nitrosum poisoning anoxic time-to-live and the mouse weightening finish is not had influence.
2.3 said health-care edible capsule is to the influence of test of chmice acute cerebral hypoxia ischemia and mouse weightening finish
The said health-care edible capsule of table 3. is to the influence of test of chmice acute cerebral hypoxia ischemia and mouse weightening finish
P: each experimental group and negative control group be * p<0.05 relatively
Visible by table 3; Per os gives the said health-care edible capsule 30 days of mouse various dose; Each is organized the mouse weightening finish and compares there was no significant difference (p>0.05) with negative control group; Dehisce to breathe dwell time than the obvious prolongation of negative control group (p<0.05) behind the middle dose groups mouse broken end, dehisce to breathe when stopping behind the high and low dose group mouse broken end
Between with negative control group there was no significant difference (p>0.05) relatively.
3, brief summary
Per os gives the said health-care edible capsule of mouse 0.05,0.50,1.50g/kg BW d, continuous irrigation stomach 30 days, and high dose group can prolong the normobaric hypoxia anoxia in mice time-to-live; Middle and high dosage can prolong natrium nitrosum and poison the back time-to-live; Middle dose groups can prolong acute cerebral ischemia property anoxic and dehisce the time of breathing.Judge that thus said health-care edible capsule has the anoxia in mice of raising tolerance function in range of doses.