CN101591624B - Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta - Google Patents
Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta Download PDFInfo
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- CN101591624B CN101591624B CN2009100508179A CN200910050817A CN101591624B CN 101591624 B CN101591624 B CN 101591624B CN 2009100508179 A CN2009100508179 A CN 2009100508179A CN 200910050817 A CN200910050817 A CN 200910050817A CN 101591624 B CN101591624 B CN 101591624B
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- microbial inoculum
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- animal excreta
- flask culture
- complex microbial
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- 210000003608 fece Anatomy 0.000 title claims abstract description 10
- 241001465754 Metazoa Species 0.000 title claims abstract description 9
- 239000002068 microbial inoculum Substances 0.000 title claims abstract description 9
- 238000000034 method Methods 0.000 title claims description 7
- 238000012545 processing Methods 0.000 title abstract description 5
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 9
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 9
- 244000168141 Geotrichum candidum Species 0.000 claims abstract description 9
- 235000017388 Geotrichum candidum Nutrition 0.000 claims abstract description 9
- 238000002360 preparation method Methods 0.000 claims abstract description 8
- 238000012807 shake-flask culturing Methods 0.000 claims abstract description 8
- 239000007787 solid Substances 0.000 claims abstract description 7
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 8
- 241000194017 Streptococcus Species 0.000 claims description 8
- 241000187091 Streptomyces diastaticus Species 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229920001817 Agar Polymers 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 239000008272 agar Substances 0.000 claims description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Natural products OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- 239000001888 Peptone Substances 0.000 claims description 2
- 108010080698 Peptones Proteins 0.000 claims description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 229940041514 candida albicans extract Drugs 0.000 claims description 2
- -1 citric acid diamines Chemical class 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 238000002386 leaching Methods 0.000 claims description 2
- 235000019319 peptone Nutrition 0.000 claims description 2
- 108010009004 proteose-peptone Proteins 0.000 claims description 2
- 239000001632 sodium acetate Substances 0.000 claims description 2
- 235000017281 sodium acetate Nutrition 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 239000012138 yeast extract Substances 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims 3
- 239000000417 fungicide Substances 0.000 claims 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims 1
- 229920000053 polysorbate 80 Polymers 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 2
- 239000004382 Amylase Substances 0.000 abstract 1
- 102000013142 Amylases Human genes 0.000 abstract 1
- 108010065511 Amylases Proteins 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 abstract 1
- 241000194020 Streptococcus thermophilus Species 0.000 abstract 1
- 241000187747 Streptomyces Species 0.000 abstract 1
- 235000019418 amylase Nutrition 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 description 11
- 150000001875 compounds Chemical class 0.000 description 10
- 239000002054 inoculum Substances 0.000 description 10
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000010040 Sprains and Strains Diseases 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000003716 rejuvenation Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to an efficient complex microbial inoculum for processing human and animal excreta, which consists of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcus thermophilus, geotrichum candidum, aspergillus niger and heat amylase streptomyces. A preparation method comprises the following steps of first-stage shake flask culture, second-stage shake flask culture, and third-stage solid medium culture of the complex microbial inoculum for the strains. The preparation method is simple with low use cost, saves resources, can be widely applied to stationary type or wheeled lavatories, and accords with the policy requirement of sustainable development.
Description
Technical field
The invention belongs to the ecological, environmental protective field, relate to a kind of preparation method who handles the effectively compound microbial inoculant of human and animal excreta.
Background technology
The lavatory plays an important role in daily life, and its admittance every day, accumulation are also got rid of excretory overwhelming majority pollutent in the human daily physiological activity.These excrement dirts have become the main source of pollutent in the sanitary sewage after entering municipal sewage system, also are the important component parts of municipal effluent pollutent.
Along with development of science and technology, many recycling processing methods and the artificial culture microorganism that is applied to wherein of being absorbed in human and animal excreta are all arranged at present both at home and abroad, but the many existence of these microbial strainss are too single, production prepares too problems such as complexity, cost height, and treatment effect is undesirable can't be promoted the use of thereby cause.
Summary of the invention
The purpose of this invention is to provide a kind of obvious processing effect, do not cause secondary pollution, can overcome the shortcoming of prior art, can be widely used in the processing of fixed or wheeled lavatory.
The present invention is a kind of effectively compound microbial inoculant of handling human and animal excreta, it is characterized in that being made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, it is 3: 2: 5 that this seven kinds of bacterial strains bacterium in every gram microbial inoculum is counted the scope ratio: 3: 3: 3: 4.
Effectively compound microbial inoculant of the present invention, it is characterized in that its cultural method is: at first subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger, hot streptomyces diastaticus are made successively primary inclined plane cultivation, one-level shake-flask culture, secondary shake-flask culture respectively, thalline is transplanted in solid medium cultivated then under 35 ℃ of room temperatures.
Effectively compound microbial inoculant of the present invention is characterized in that described slant medium is peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
Effectively compound microbial inoculant of the present invention is characterized in that described solid medium is casein peptone 10.0g, and extractum carnis 10.0g, yeast extract are 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamines 2.0g, soil temperature 801.0g, K
2HPO
42.0g, MgSO
47H
2O 0.2g, MnSO
4H
2O 0.05g, CaCO
32 0.0g, agar 15.0g, distilled water 1.0L, pH6.8.
Effectively compound microbial inoculant of the present invention is characterized in that described solid base is made up of sawdust, rice bran, Semen Maydis powder, soyflour and wheat bran, and their weight ratio is 1: 1: 1: 1: 1.
In the present invention, its using method is: with the biologic packing material thorough mixing, as the treatment agent of ight soil.
The used bacterial classification of the present invention comes from the contriver from the various avirulent strains that the Chinese Academy of Sciences biological preservation center is bought and process monitoring at different levels department identifies, transferred species rejuvenation regularly and evaluation.
Advantage of the present invention is: the processing efficiency height of bacterial classification, used meet microorganism and can utilize multiple organism in the ight soil to ferment and breathe the metabolism that has concurrently, growth temperature taken into account low in the high-temperature stage, realized removing fast of moisture and stink in the ight soil.The preparation method is easy, and the sawdust of employing low price, wide material sources, rice bran, Semen Maydis powder, soyflour etc. are simple, with low cost,
Embodiment 1
Handle the effectively compound microbial inoculant of human and animal excreta and be made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, the ratio between the various microorganisms is a subtilis: Bacillus licheniformis: stearothermophilus ground bacillus: thermophilus streptococcus: geotrichum candidum: aspergillus niger: hot streptomyces diastaticus=3: 2: 5: 3: 3: 3: 4.
This bacterial classification adopts following method to cultivate: microbial inoculum is made primary inclined plane cultivations, one-level shake-flask culture, secondary shake-flask culture successively, then thalline was transplanted in solid medium cultivation 96 hours under 35 ℃ of room temperatures.
During use, cultured effectively compound microbial flora is inoculated field planting with biologic packing material under 35 ℃ temperature in special container, filler is placed in the bio-toilet stool storage box, be used for the biological treatment in this lavatory.
These bio-toilet 3 crouching positions, 800 person-times/day of numbers of users.Normal-temperature aerobic.Treat effluent five days biochemical oxygen demand (BOD5) is 12.2mg/L, and chemical oxygen demand (COD) (CODcr) is 33.7mg/L, and ammonia nitrogen, suspended substance, the intestinal bacteria number average is up to standard, water outlet is light brown.
Embodiment 2
Handle the effectively compound microbial inoculant of human and animal excreta and be made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, the ratio between the various microorganisms is a subtilis: Bacillus licheniformis: stearothermophilus ground bacillus: thermophilus streptococcus: geotrichum candidum: aspergillus niger: hot streptomyces diastaticus=3: 2: 5: 3: 3: 3: 4.
The cultural method of microbial inoculum is with embodiment 1.
Freshly-slaughtered poultry excrement, birds droppings are placed fermenter, add the sawdust mixing, adjusting moisture to 45%, acquisition pre-treatment material by weight 30%.Under 35 ℃,, carry out timing and stir to wherein adding the mentioned microorganism microbial inoculum with the inoculum size of weight ratio 5%, to ferment 7 days, water content is reduced to about 20%, and outward appearance is a grey, slightly the vinasse smell.
Claims (3)
1. preparation method who handles the complex micro organism fungicide of human and animal excreta, it is characterized in that its cultural method is: at first subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger, hot streptomyces diastaticus are made successively primary inclined plane cultivation, one-level shake-flask culture, secondary shake-flask culture respectively, thalline is transplanted in solid medium cultivated then under 35 ℃ of room temperatures; It is that the bacterium ratio of counting of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger, hot streptomyces diastaticus in every gram microbial inoculum is 3: 2: 5 that the bacterium of described microbial inoculum is counted the scope ratio: 3: 3: 3: 4.
2. the preparation method of the described complex micro organism fungicide of claim 1 is characterized in that described primary inclined plane is peptone 10.0g, beef leaching thing 3.0g, NaCl 5.0g, agar 15.0g, distilled water 1.0L, pH7.0.
3. the preparation method of the described complex micro organism fungicide of claim 1 is characterized in that described solid medium is casein peptone 10.0g, extractum carnis 10.0g, yeast extract is 5.0g, glucose 5.0g, sodium acetate 5.0g, citric acid diamines 2.0g, tween 80 1.0g, K
2HPO
42.0g, MgSO
47H
2O 0.2g, MnSO
4H
2O 0.05g, CaCO
320.0g, agar 15.0g, distilled water 1.0L, pH6.8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100508179A CN101591624B (en) | 2009-05-08 | 2009-05-08 | Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100508179A CN101591624B (en) | 2009-05-08 | 2009-05-08 | Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta |
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| Publication Number | Publication Date |
|---|---|
| CN101591624A CN101591624A (en) | 2009-12-02 |
| CN101591624B true CN101591624B (en) | 2011-09-28 |
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| CN2009100508179A Expired - Fee Related CN101591624B (en) | 2009-05-08 | 2009-05-08 | Method for preparing high-efficiency complex microbial inoculum for processing human and animal excreta |
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| CN104211277A (en) * | 2014-09-11 | 2014-12-17 | 山东爱福地生物科技有限公司 | Deodorizing process and device for pretreating livestock and poultry feces by utilizing decomposition agent |
| CN104480044A (en) * | 2014-12-13 | 2015-04-01 | 佛山市高明区合朝农业发展有限公司 | Microbial compound agent for preparing bio-organic fertilizers with excrement |
| CN104531586B (en) * | 2014-12-31 | 2018-09-14 | 广州舒国生物科技有限公司 | A kind of microbial bacterial agent and its preparation method and application of processing spoil |
| CN104630115A (en) * | 2015-02-11 | 2015-05-20 | 上海新张卫生用品有限公司 | Microbial inoculant applicable to in-situ treatment of biochemical mobile toilets |
| CN108947164B (en) * | 2016-01-22 | 2021-05-07 | 浙江佳净洁环境科技有限公司 | Application method of compound microbial preparation |
| CN106995788A (en) * | 2016-01-22 | 2017-08-01 | 江苏佳净洁环境科技有限公司 | A kind of complex microorganism preparations and preparation method and application for decomposing human feces |
| CN105585235B (en) * | 2016-01-22 | 2019-06-18 | 浙江佳净洁环境科技有限公司 | A kind of complex microorganism preparations and preparation method and application of deodorization |
| CN107118995B (en) * | 2017-06-29 | 2019-08-23 | 重庆市万源禽蛋食品有限公司 | Microbial fermentation inoculum and its application and the livestock manure fermented method for applying it |
| CN109439608A (en) * | 2018-11-16 | 2019-03-08 | 安徽骏旌环保科技有限公司 | A kind of duck class excrement decomposition culture medium |
| CN109439610A (en) * | 2018-11-16 | 2019-03-08 | 安徽骏旌环保科技有限公司 | A kind of human feces decomposition culture medium |
| CN109825460A (en) * | 2019-03-29 | 2019-05-31 | 上海华杰生态环境工程有限公司 | The composite bacteria agent and preparation method thereof of Rapid Fermentation human and animal excreta |
| CN110698275A (en) * | 2019-10-18 | 2020-01-17 | 惠州市乐夫农业科技有限公司 | Microbial agent |
| CN110668868A (en) * | 2019-10-18 | 2020-01-10 | 惠州市乐夫农业科技有限公司 | High-efficiency biological fertilizer |
| CN113999790A (en) * | 2021-10-11 | 2022-02-01 | 河南国昊技术有限公司 | Microbial composite sheet for deodorizing and degrading excrement of intelligent wheelchair and preparation method thereof |
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- 2009-05-08 CN CN2009100508179A patent/CN101591624B/en not_active Expired - Fee Related
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