CN101586088B - Effectively compound microbial inoculant for processing human and animal excreta - Google Patents

Effectively compound microbial inoculant for processing human and animal excreta Download PDF

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Publication number
CN101586088B
CN101586088B CN2009100505819A CN200910050581A CN101586088B CN 101586088 B CN101586088 B CN 101586088B CN 2009100505819 A CN2009100505819 A CN 2009100505819A CN 200910050581 A CN200910050581 A CN 200910050581A CN 101586088 B CN101586088 B CN 101586088B
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compound microbial
animal excreta
effectively compound
human
bacillus
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CN101586088A (en
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马志恒
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Shanghai Huajie Ecological Environment Engineering Co Ltd
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Shanghai Huajie Ecological Environment Engineering Co Ltd
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Abstract

The invention is a compound microbial inoculant that processes human and animal excreta effectively composed of bacillus subtilis, bacillus licheniformis, geobacillus stearothermophilus, streptococcusthermophilus, geotrichum candidum, aspergillus niger and thermal amylase streptomycete. The microbial inoculum uses metabolism to decompose human and animal excreta effectively in short period of tim e effectively without secondary pollution during process, which extremely reduces environmental pollution brought by human and animal excreta. Meanwhile, the method is sample, low in cost, and saves resources, which can be widely applied to fixed or movable rest rooms, matching the policies of sustainable development.

Description

A kind of effectively compound microbial inoculant of handling human and animal excreta
Technical field
The invention belongs to the ecological, environmental protective field, relate to a kind of effectively compound microbial of handling human and animal excreta.
Background technology
The lavatory plays an important role in daily life, and its admittance every day, accumulation are also got rid of excretory overwhelming majority pollutent in the human daily physiological activity.These excrement dirts have become the main source of pollutent in the sanitary sewage after entering municipal sewage system, also are the important component parts of municipal effluent pollutent.
Along with development of science and technology, many recycling processing methods and the artificial culture microorganism that is applied to wherein of being absorbed in human and animal excreta are all arranged at present both at home and abroad, exist or too single or problems such as complexity, cost height too but these microbial strainss, treatment effect is undesirable can't be promoted the use of thereby cause.
Summary of the invention
The purpose of this invention is to provide a kind of obvious processing effect, do not cause secondary pollution, can overcome the shortcoming of prior art, can be widely used in the processing of fixed or wheeled lavatory.
The present invention is a kind of effectively compound microbial inoculant of handling human and animal excreta, it is characterized in that being made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, it is 3: 2: 5 that this seven kinds of bacterial strains bacterium in every gram microbial inoculum is counted the scope ratio: 3: 3: 3: 4.
In the present invention, its using method is: with the biologic packing material thorough mixing, as the treatment agent of ight soil.
In the present invention, the method of spawn culture is: at first subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger, hot streptomyces diastaticus are made successively primary inclined plane cultivation, one-level shake-flask culture, secondary shake-flask culture respectively, thalline is transplanted in solid medium under 35 ℃ of room temperatures cultivated then.
The used bacterial classification of the present invention comes from the contriver from the various avirulent strains that the Chinese Academy of Sciences biological preservation center is bought and process monitoring at different levels department identifies, transferred species rejuvenation regularly and evaluation.
Advantage of the present invention is: the processing efficiency height, used meet microorganism and can utilize multiple organism in the ight soil to ferment and breathe the metabolism that has concurrently, growth temperature has been taken into account the low middle high-temperature stage, realized removing fast of moisture and stink in the ight soil, simultaneously waste can be used as the high-quality fertilizer and uses, and method is simple, realized the resource circulation utilization.
Embodiment 1
Handle the effectively compound microbial inoculant of human and animal excreta and be made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, the ratio between the various microorganisms is a subtilis: Bacillus licheniformis: stearothermophilus ground bacillus: thermophilus streptococcus: geotrichum candidum: aspergillus niger: hot streptomyces diastaticus=3: 2: 5: 3: 3: 3: 4.
This bacterial classification adopts following method to cultivate: microbial inoculum is made primary inclined plane cultivations, one-level shake-flask culture, secondary shake-flask culture successively, then thalline was transplanted in solid medium cultivation 96 hours under 35 ℃ of room temperatures.
During use, cultured effectively compound microbial flora is inoculated field planting with biologic packing material under 35 ℃ temperature in special container, filler is placed in the bio-toilet stool storage box, be used for the biological treatment in this lavatory.
These bio-toilet 3 crouching positions, 800 person-times/day of numbers of users.Normal-temperature aerobic.Treat effluent five days biochemical oxygen demand (BOD5) is 12.2mg/L, and chemical oxygen demand (COD) (CODcr) is 33.7mg/L, and ammonia nitrogen, suspended substance, the intestinal bacteria number average is up to standard, water outlet is light brown.
Embodiment 2
Handle the effectively compound microbial inoculant of human and animal excreta and be made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus, the ratio between the various microorganisms is a subtilis: Bacillus licheniformis: stearothermophilus ground bacillus: thermophilus streptococcus: geotrichum candidum: aspergillus niger: hot streptomyces diastaticus=3: 2: 5: 3: 3: 3: 4.
The cultural method of microbial inoculum is with embodiment 1.
Freshly-slaughtered poultry excrement, birds droppings are placed fermenter, add the sawdust mixing, adjusting moisture to 45%, acquisition pre-treatment material by weight 30%.Under 35 ℃,, carry out timing and stir to wherein adding the mentioned microorganism microbial inoculum with the inoculum size of weight ratio 5%, to ferment 7 days, water content is reduced to about 20%, and outward appearance is a grey, slightly the vinasse smell.

Claims (4)

1. an effectively compound microbial inoculant of handling human and animal excreta is characterized in that being made up of subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger and hot streptomyces diastaticus.
2. the effectively compound microbial inoculant of processing human and animal excreta as claimed in claim 1 is characterized in that the bacterium ratio of counting between the various microorganisms is a subtilis: Bacillus licheniformis: stearothermophilus ground bacillus: thermophilus streptococcus: geotrichum candidum: aspergillus niger: hot streptomyces diastaticus=3: 2: 5: 3: 3: 3: 4.
3. claim 1 or 2 described effectively compound microbial inoculants is characterized in that its using method is: cultured in advance effectively compound microbial flora and biologic packing material thorough mixing are used for fixing formula or wheeled lavatory and use as the treatment agent of ight soil.
4. claim 1 or 2 described effectively compound microbial inoculants, it is characterized in that its cultural method is: at first subtilis, Bacillus licheniformis, stearothermophilus ground bacillus, thermophilus streptococcus, geotrichum candidum, aspergillus niger, hot streptomyces diastaticus are made successively primary inclined plane cultivation, one-level shake-flask culture, secondary shake-flask culture respectively, thalline is transplanted in solid medium cultivated then under 35 ℃ of room temperatures.
CN2009100505819A 2009-05-05 2009-05-05 Effectively compound microbial inoculant for processing human and animal excreta Active CN101586088B (en)

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Families Citing this family (9)

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Publication number Priority date Publication date Assignee Title
CN101717722B (en) * 2009-11-30 2012-08-22 中国农业科学院农业环境与可持续发展研究所 Microbe microbial inoculum, preparation method thereof and method for producing biological humic acid
CN104630115A (en) * 2015-02-11 2015-05-20 上海新张卫生用品有限公司 Microbial inoculant applicable to in-situ treatment of biochemical mobile toilets
CN106995788A (en) * 2016-01-22 2017-08-01 江苏佳净洁环境科技有限公司 A kind of complex microorganism preparations and preparation method and application for decomposing human feces
CN106513416A (en) * 2016-10-31 2017-03-22 南开大学 A technical method for mixed refuse sorting and deodorizing
CN108486003A (en) * 2018-03-15 2018-09-04 同济大学 A kind of microbial fermentation bacterium of fast deodorization fixed nitrogen type processing human and animal excreta
CN109880773A (en) * 2019-03-29 2019-06-14 上海华杰生态环境工程有限公司 The complex microorganism preparations and preparation method thereof of efficient-decomposition human and animal excreta
CN109825460A (en) * 2019-03-29 2019-05-31 上海华杰生态环境工程有限公司 The composite bacteria agent and preparation method thereof of Rapid Fermentation human and animal excreta
CN112625959B (en) * 2020-12-28 2023-06-23 中农新科(苏州)有机循环研究院有限公司 Thermophilic composite microbial inoculant for aerobic fermentation of organic waste
CN112812990A (en) * 2020-12-31 2021-05-18 宜兴艾科森生态环卫设备有限公司 Compound microbial agent for excrement treatment and application and compounding method thereof

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