CN104531586B - A kind of microbial bacterial agent and its preparation method and application of processing spoil - Google Patents
A kind of microbial bacterial agent and its preparation method and application of processing spoil Download PDFInfo
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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Abstract
The invention discloses a kind of microbial bacterial agents and its preparation method and application of processing spoil.The microbial bacterial agent of the processing spoil of the present invention is by 5 plants of strains according to the complex micro organism fungicide formed after certain mixing than row, after being mixed with the spoil rubbed according to corresponding amount, at a temperature of 70 90 DEG C, in for 24 hours almost can degradable spoil, and be converted into available microbial organic fertilizer.It solves spoil quick resource, innoxious and minimizing sciences problems in situ, there is extraordinary application prospect.
Description
Technical field：
The invention belongs to spoil harmless treatment fields, and in particular to a kind of microbial bacterial agent of processing spoil
And its preparation method and application.
Background technology：
Animal husbandry is fast-developing, die of illness, the improper spoil to die of illness it is increasing, it has also become influence current animal epidemic disease
The outstanding problem of sick prevention and control and sustainable development of animal husbandry.Harmless treatment is carried out to these spoils, is related to food peace
Entirely, public health security, Environmental security and animal husbandry are sustainable, develop in a healthy way, and make that conservation culture, to build city type existing
The important process of generation agricultural and city of the world.Spoil arbitrarily is abandoned, there is potential transmission harm, once it will carry
The spoil of infecting both domestic animals and human encephalapthy agent is discarded in the even residential block dustbin, river, Hu Bo, it will seriously endangers the mankind's
Health, the even spoil of natural death, also can cause environmental pollution.For production of developing animal husbandry, ensure people group
Many health implement the urgent task that animals died of illness harmless treatment has become current urgent need to resolve.How dead livestock and poultry is made
Science, low-carbon and quickly processing, the key technical problem in being innoxious and recycling treatment.
Existing innoxious and resource treatment technique respectively has advantage and disadvantage, according to investigations, what microorganism and instrument and equipment combined
Method is most widely used in present spoil processing.And in this technique, the function of microorganism is spoil degradation speed
The critical issue of valuable resource is spent and converts, so it is spoil processing to develop, develop H.D microbial bacterial agent
An important ring for middle recycling.
Invention content：
The purpose of the present invention is to provide one kind can fast degradation spoil, be converted to organic fertilizer, realize animal
The microbial bacterial agent for the processing spoil that corpse quick resource, innoxious and minimizing are handled.
The microbial bacterial agent of the processing spoil of the present invention, which is characterized in that the microbial bacterial agent is by compound bacteria
Liquid and carrier are according to mass ratio 1：0.5~1.5 mixes, and aspergillus oryzae (Asperqillus is contained in the composite bacteria liquid
Oryzae) CICC20142.0~3.0 × 1012A/L, streptococcus thermophilus (Streptococcus thermophilus) CICC
2011.0~3.0 × 1013A/L, saccharomycete (Saccharomyces cerevisiae) CICC 10021.0~3.0 × 1013
A/L, actinomyces (Thermoactinomyces sp) CICC 105233.0~3.5 × 1012A/L and bacillus licheniformis
(Bacillus licheniformis) CICC 100921.0~2.5 × 1013A/L；The carrier is by corn flour and rice bran
According to mass ratio 1:2.5~3.5 mix.
It is preferred that the microbial bacterial agent by composite bacteria liquid and carrier according to mass ratio 1：1 mixes, the carrier
By corn flour and rice bran according to mass ratio 1:3 mix.
Second object of the present invention is to provide a kind of preparation method of the microbial bacterial agent of processing spoil, feature
It is, includes the following steps：
A, aspergillus oryzae (Asperqillus oryzae) CICC 2014, streptococcus thermophilus are cultivated respectively
(Streptococcus thermophilus) CICC 201, saccharomycete (Saccharomyces cerevisiae) CICC
1002, actinomyces (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus
Licheniformis) 10092 CICC obtain its bacterium solution, then by aspergillus oryzae (Asperqillus oryzae) CICC
2014, streptococcus thermophilus (Streptococcus thermophilus) CICC 201, saccharomycete (Saccharomyces
Cerevisiae) CICC 1002, actinomyces (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis
10092 bacterium solutions of (Bacillus licheniformis) CICC are mixed to form composite bacteria liquid so that contain meter Qu in composite bacteria liquid
Mould (Asperqillus oryzae) CICC 20142.0~3.0 × 1012A/L, streptococcus thermophilus (Streptococcus
Thermophilus) 2011.0~3.0 × 10 CICC13A/L, saccharomycete (Saccharomyces cerevisiae) CICC
10021.0~3.0 × 1013A/L, actinomyces (Thermoactinomyces sp) CICC 105233.0~3.5 × 1012A/
L and bacillus licheniformis (Bacillus licheniformis) CICC 100921.0~2.5 × 1013A/L；
B, by corn flour and rice bran according to mass ratio 1:2.5~3.5 be uniformly mixed form carrier；
C, by composite bacteria liquid and carrier according to mass ratio 1：0.5~1.5 is uniformly mixed the micro- life for forming processing spoil
Object microbial inoculum.
It is preferred that the microbial bacterial agent by composite bacteria liquid and carrier according to mass ratio 1：1 mixes, the carrier
By corn flour and rice bran according to mass ratio 1:3 mix.
Third object of the present invention is to provide the microbial bacterial agents of above-mentioned processing spoil in spoil of degrading
Application.
The microbial bacterial agent of the processing spoil of the present invention is to answer 5 plants of strains according to what is formed after certain mixing than row
Microbial bacterial agent is closed, it is interior for 24 hours almost at a temperature of 70-90 DEG C after being mixed with the spoil rubbed according to corresponding amount
Degradable spoil, and it is converted into available microbial organic fertilizer.Solves spoil fast resource in situ
Change, innoxious and minimizing sciences problems, there is extraordinary application prospect.
Specific implementation mode：
The following examples are further illustrations of the invention, rather than limiting the invention.
1, the preparation of aspergillus oryzae bacterium solution
Aspergillus oryzae (Asperqillus oryzae CICC 2014) is chosen a ring from aspergillus oryzae slant medium to connect
In kind to liquid aspergillus oryzae seed culture medium, in 22~28 DEG C, 12~48h of static gas wave refrigerator, when bacterial strain is in exponential phase,
Stop culture, aspergillus oryzae first class inoculum is made；Then aspergillus oryzae seed culture medium is filled by the access of the inoculum concentration of 1~5%v/v
In fermentation tank, temperature is 22~28 DEG C, 24~72 hours static sealed fermenting time, obtains aspergillus oryzae (Asperqillus
Oryzae) 2014 bacterium solutions of CICC, every liter of 150mL containing brewer's wort of aspergillus oryzae slant medium, the agar 20g, surplus are
Water, pH are natural.Described every liter of 100-300ml containing brewer's wort of aspergillus oryzae seed culture medium, 3~5g of glucose, surplus is water,
PH6.5~7.5.
Each culture medium in embodiment be all by each component after mixing, sterilizing it is spare, similarly hereinafter.
2, the preparation of streptococcus thermophilus fermentation liquid
The bacterium of described streptococcus thermophilus (Streptococcus thermophilus) CICC 201 is by with lower section
Prepared by method：Streptococcus thermophilus is chosen into a ring from streptococcus thermophilus slant medium and is seeded to liquid lactobacillus species sub- culture
In base, in 28~37 DEG C, 24~60h of stationary culture, when bacterial strain is in exponential phase, stops culture, lactobacillus one is made
Then grade strain is accessed in the fermentation tank for filling lactobacillus seed culture medium, temperature 35~40 by the inoculum concentration of 1~5%v/v
DEG C, fermentation time 24~72 hours obtains 201 bacterium solutions of streptococcus thermophilus (Streptococcus thermophilus) CICC,
Described every liter of 100.0g containing skimmed milk power of streptococcus thermophilus slant medium, yeast powder 1g, K2HPO45g, agar 20g, surplus
For water, pH is 6~7；Described every liter of 2~3g containing peptone of lactobacillus seed culture medium, 2~3g of glucose, surplus is water,
PH6.0~7.0.
3, the preparation of saccharomyces cerevisiae bacterium solution
The bacterium of described saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC 1002 is to make by the following method
Standby：Saccharomyces cerevisiae (Saccharomyces cerevisiae) CICC 1002 is chosen from candidiasis slant medium
A ring is gone to be seeded in liquid S. cervisiae seed culture medium, in 28~37 DEG C, 150~200rpm, shaken cultivation 12~
48h stops culture when bacterial strain is in exponential phase, and S. cervisiae first class inoculum is made；Then press 1~5%v/v's
Inoculum concentration accesses in the fermentation tank for filling S. cervisiae seed culture medium, and temperature is 28~35 DEG C, and speed of agitator 200~
300rpm, 2~4L/ of ventilatory capacity (Lmin), fermentation time 24~72 hours, obtain saccharomyces cerevisiae (Saccharomyces
Cerevisiae) 1002 bacterium solutions of CICC, described every liter of 5g containing glucose of S. cervisiae slant medium, peptone 2g, ferment
Female cream 1g, sodium chloride 0.5g, agar 20g, surplus are water, and pH is 6.5~7.5；The S. cervisiae seed culture medium is every
3~4g containing peptone, 7~9g of yeast extract, 3~5g of glucose are risen, surplus is water, pH6.5~7.5.
4, the preparation of actinomyces bacterium solution
The bacterium of described actinomyces (Thermoactinomyces sp) CICC 10523 is prepared by the following method：
Actinomyces (Thermoactinomyces sp) CICC 10523 is chosen into ring inoculation from candidiasis slant medium
Into liquid actinomyces bacterium seed culture medium, waited at bacterial strain in 28~37 DEG C, 150~200rpm, 12~48h of shaken cultivation
When exponential phase, stop culture, actinomyces bacterium first class inoculum is made；Then the inoculum concentration access for pressing 1~5%v/v is contained
In the fermentation tank for having actinomyces bacterium seed culture medium, temperature is 28~35 DEG C, 200~300rpm of speed of agitator, ventilatory capacity 2~
4L/ (Lmin), fermentation time 24~72 hours obtain 10523 bacterium of actinomyces (Thermoactinomyces sp) CICC
Liquid, candidiasis slant medium every liter of 0.50g containing yeast powder, peptone 0.50g, the casein hydrolysate 0.50g,
Glucose 0.50g, soluble starch 0.50g, Sodium Pyruvate 0.30g, KH2PO40.30g, MgSO4·7H2O 0.05g, agar
15g, distilled water 1000mL, final pH 7.2；Described every liter of 3~4g of powder containing brewer's wort of actinomyces bacterium seed culture medium, yeast
7~9g of cream, surplus are water, pH6.5~7.5.
5, the preparation of bacillus licheniformis bacterium solution
Described bacillus licheniformis (Bacillus licheniformis) CICC 10092 is to make by the following method
Standby：By bacillus licheniformis (Bacillus licheniformis) CICC 10092 from bacillus licheniformis slant medium
On choose a ring and be seeded in liquid bacillus licheniformis seed culture medium, in 28~37 DEG C, 150~230rpm, shaken cultivation
24~48h stops culture when bacterial strain is in exponential phase, and bacillus licheniformis first class inoculum is made, and then presses 1~5%
The inoculum concentration access of v/v fills in the fermentation tank of bacillus licheniformis seed culture medium, 28~35 DEG C of temperature, speed of agitator 250
~350rpm, 1~2L/ of ventilatory capacity (Lmin), fermentation time 24~72 hours, obtain bacillus licheniformis (Bacillus
Licheniformis) CICC10092 bacterium solutions, described every liter of 9~11g containing peptone of bacillus licheniformis slant medium, ox
4.5~5.5g of meat extract, 9~11g of glucose, 4.5~5.5g of sodium chloride, agar 20g, surplus are water, and pH is 6.5~7.5, described
Every liter of 4~5g containing peptone of bacillus licheniformis seed culture medium, 2~3g of yeast extract, 3~5g of glucose, surplus is water,
PH7.0~8.5.
Embodiment 1：
By 2014 bacterium solutions of above-mentioned aspergillus oryzae (Asperqillus oryzae) CICC, streptococcus thermophilus
201 bacterium solutions of (Streptococcus thermophilus) CICC, saccharomycete (Saccharomyces cerevisiae) CICC
1002 bacterium solutions, 10523 bacterium solutions of actinomyces (Thermoactinomyces sp) CICC and bacillus licheniformis (Bacillus
Licheniformis) 10092 bacterium solutions of CICC are mixed to form composite bacteria liquid so that contain aspergillus oryzae in composite bacteria liquid
(Asperqillus oryzae)CICC 20142.0×1012A/L, streptococcus thermophilus (Streptococcus
thermophilus)CICC 2011.0×1013A/L, saccharomycete (Saccharomyces cerevisiae) CICC
10021.0×1013A/L, actinomyces (Thermoactinomyces sp) CICC 105233.0 × 1012A/L and lichens bud
Born of the same parents bacillus (Bacillus licheniformis) CICC 100921.0 × 1013A/L.
By corn flour and rice bran according to mass ratio 1:3.5 ratio is uniformly mixed obtained carrier.
By composite bacteria liquid and carrier according to mass ratio 1：1.5 are uniformly mixed, and obtain the microbial bacterial agent of processing spoil.
The microbial bacterial agent that the processing spoil of 1.2kg is added in 600kg spoils, is uniformly mixed, is placed at
At 70 DEG C, rear remaining spoil is 80kg for 24 hours, reduces 520kg, reduction rate 520/600*100%=90%, animal
Corpse is converted into available microbial organic fertilizer.Solve spoil quick resource, innoxious and decrement in situ
The sciences problems of change.
Embodiment 2：
By 2014 bacterium solutions of above-mentioned aspergillus oryzae (Asperqillus oryzae) CICC, streptococcus thermophilus
201 bacterium solutions of (Streptococcus thermophilus) CICC, saccharomycete (Saccharomyces cerevisiae) CICC
1002 bacterium solutions, 10523 bacterium solutions of actinomyces (Thermoactinomyces sp) CICC and bacillus licheniformis (Bacillus
Licheniformis) 10092 bacterium solutions of CICC are mixed to form composite bacteria liquid so that contain aspergillus oryzae in composite bacteria liquid
(Asperqillus oryzae)CICC 20143.0×1012A/L, streptococcus thermophilus (Streptococcus
thermophilus)CICC 2013.0×1013A/L, saccharomycete (Saccharomyces cerevisiae) CICC
10023.0×1013A/L, actinomyces (Thermoactinomyces sp) CICC 105233.5 × 1012A/L and lichens bud
Born of the same parents bacillus (Bacillus licheniformis) CICC 100922.5 × 1013A/L；
By corn flour and rice bran according to mass ratio 1:2.5 ratio is uniformly mixed obtained carrier.
By composite bacteria liquid and carrier according to mass ratio 1：0.5 is uniformly mixed, and obtains the microbial bacterial agent of processing spoil.
The microbial bacterial agent that the processing spoil of 1.67kg is added in 835kg spoils, is uniformly mixed, is placed at
At 90 DEG C, rear remaining spoil is 120kg for 24 hours, reduces 715kg, reduction rate 715/835*100%=85.63%,
Spoil is converted into available microbial organic fertilizer.To it is in situ solve spoil quick resource, it is innoxious and
The sciences problems of minimizing.
Embodiment 3：
By 2014 bacterium solutions of aspergillus oryzae (Asperqillus oryzae) CICC, streptococcus thermophilus (Streptococcus
Thermophilus) 201 bacterium solutions of CICC, 1002 bacterium solutions of saccharomycete (Saccharomyces cerevisiae) CICC, unwrapping wire
10523 bacterium solutions of bacterium (Thermoactinomyces sp) CICC and bacillus licheniformis (Bacillus licheniformis)
10092 bacterium solutions of CICC are mixed to form composite bacteria liquid so that contain aspergillus oryzae (Asperqillus oryzae) in composite bacteria liquid
CICC 20142.5×1012A/L, streptococcus thermophilus (Streptococcus thermophilus) CICC 2012 × 1013
A/L, saccharomycete (Saccharomyces cerevisiae) CICC 10022 × 1013A/L, actinomyces
(Thermoactinomyces sp)CICC 105233.2×1012A/L and bacillus licheniformis (Bacillus
licheniformis)CICC 100922×1013A/L；
By corn flour and rice bran according to mass ratio 1:3 ratio is uniformly mixed obtained carrier.
By composite bacteria liquid and carrier according to mass ratio 1：1 is uniformly mixed, and obtains the microbial bacterial agent of processing spoil.
The microbial bacterial agent that the processing spoil of 1.446kg is added in 723kg spoils, is uniformly mixed, is placed in
At 80 DEG C, rear remaining spoil is 48kg for 24 hours, reduces 675kg, reduction rate 675/723*100%=
93.36%, spoil is converted into available microbial organic fertilizer.To it is in situ solve spoil quick resource,
Innoxious and minimizing sciences problems.
Embodiment 4：
By 2014 bacterium solutions of aspergillus oryzae (Asperqillus oryzae) CICC, streptococcus thermophilus (Streptococcus
Thermophilus) 201 bacterium solutions of CICC, 1002 bacterium solutions of saccharomycete (Saccharomyces cerevisiae) CICC, unwrapping wire
10523 bacterium solutions of bacterium (Thermoactinomyces sp) CICC and bacillus licheniformis (Bacillus licheniformis)
10092 bacterium solutions of CICC are mixed to form composite bacteria liquid so that contain aspergillus oryzae (Asperqillus oryzae) in composite bacteria liquid
CICC 20142.6×1012A/L, streptococcus thermophilus (Streptococcus thermophilus) CICC 2012.2 × 1013
A/L, saccharomycete (Saccharomyces cerevisiae) CICC 10021.8 × 1013A/L, actinomyces
(Thermoactinomyces sp)CICC 105233.3×1012A/L and bacillus licheniformis (Bacillus
licheniformis)CICC 100922.1×1013A/L；
By corn flour and rice bran according to mass ratio 1:2.7 ratio is uniformly mixed obtained carrier.
By composite bacteria liquid and carrier according to mass ratio 1：0.6 is uniformly mixed, and obtains the microbial bacterial agent of processing spoil.
The microbial bacterial agent that the processing spoil of 1.816kg is added in 908kg spoils, is uniformly mixed, is placed in
At 80 DEG C, rear remaining spoil is 122kg for 24 hours, reduces 786kg, reduction rate 786/908*100%=
86.56%, spoil is converted into available microbial organic fertilizer.To it is in situ solve spoil quick resource,
Innoxious and minimizing sciences problems.
Claims (7)
1. a kind of microbial bacterial agent of processing spoil, which is characterized in that the microbial bacterial agent is by composite bacteria liquid and load
Body is according to mass ratio 1：0.5~1.5 mixes, and the bacterial strain in the composite bacteria liquid is by aspergillus oryzae (Asperqillus
Oryzae) CICC 2014, streptococcus thermophilus (Streptococcus thermophilus) CICC 201, saccharomycete
(Saccharomyces cerevisiae) CICC 1002,10523 Hes of actinomyces (Thermoactinomyces sp) CICC
Bacillus licheniformis (Bacillus licheniformis) CICC 10092 is formed, and each bacterial content is：Aspergillus oryzae
(Asperqillus oryzae) CICC 2,014 2.0~3.0 × 1012A/L, streptococcus thermophilus (Streptococcus
Thermophilus) 201 1.0~3.0 × 10 CICC13A/L, saccharomycete (Saccharomyces cerevisiae) CICC
1002 1.0~3.0 × 1013A/L, actinomyces (Thermoactinomyces sp) CICC 10,523 3.0~3.5 × 1012
A/L and bacillus licheniformis (Bacillus licheniformis) CICC 10,092 1.0~2.5 × 1013A/L；Described
Carrier is by corn flour and rice bran according to mass ratio 1:2.5~3.5 mix.
2. the microbial bacterial agent of processing spoil according to claim 1, which is characterized in that the microbial bacterial agent
By composite bacteria liquid and carrier according to mass ratio 1：1 mixes.
3. the microbial bacterial agent of processing spoil according to claim 1, which is characterized in that the carrier is by corn
Powder and rice bran are according to mass ratio 1:3 mix.
4. a kind of preparation method of the microbial bacterial agent of processing spoil, which is characterized in that include the following steps：
A, aspergillus oryzae (Asperqillus oryzae) CICC 2014, streptococcus thermophilus (Streptococcus are cultivated respectively
Thermophilus) CICC 201, saccharomycete (Saccharomyces cerevisiae) CICC 1002, actinomyces
(Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus licheniformis) CICC
10092, its bacterium solution is obtained, then by aspergillus oryzae (Asperqillus oryzae) CICC 2014, streptococcus thermophilus
(Streptococcus thermophilus) CICC 201, saccharomycete (Saccharomyces cerevisiae) CICC
1002, actinomyces (Thermoactinomyces sp) CICC 10523 and bacillus licheniformis (Bacillus
Licheniformis) 10092 bacterium solutions of CICC are mixed to form composite bacteria liquid so that contain aspergillus oryzae in composite bacteria liquid
(Asperqillus oryzae) CICC 2,014 2.0~3.0 × 1012A/L, streptococcus thermophilus (Streptococcus
Thermophilus) 201 1.0~3.0 × 10 CICC13A/L, saccharomycete (Saccharomyces cerevisiae) CICC
1002 1.0~3.0 × 1013A/L, actinomyces (Thermoactinomyces sp) CICC 10,523 3.0~3.5 × 1012
A/L and bacillus licheniformis (Bacillus licheniformis) CICC 10,092 1.0~2.5 × 1013A/L；
B, by corn flour and rice bran according to mass ratio 1:2.5~3.5 be uniformly mixed form carrier；
C, by composite bacteria liquid and carrier according to mass ratio 1：0.5~1.5 is uniformly mixed the microbial bacteria for forming processing spoil
Agent.
5. preparation method according to claim 4, which is characterized in that the microbial bacterial agent is by composite bacteria liquid and carrier
According to mass ratio 1：1 mixes.
6. preparation method according to claim 4, which is characterized in that the carrier is by corn flour and rice bran according to quality
Than 1:3 mix.
7. application of the microbial bacterial agent of the spoil described in claim 1-3 in spoil of degrading.
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| CN104894007B (en) * | 2015-05-12 | 2019-07-05 | 安徽全民环保科技有限公司 | Compound microbial preparation, preparation method and application in treatment of animal carcasses |
| CN105062932A (en) * | 2015-09-09 | 2015-11-18 | 肇庆大华农生物药品有限公司 | Microbial flora preparation applied to harmless dead poultry body disposal and preparation method and application thereof |
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