CN101215536A - Method for producing dejection degradation bacterium preparation - Google Patents
Method for producing dejection degradation bacterium preparation Download PDFInfo
- Publication number
- CN101215536A CN101215536A CNA200710307250XA CN200710307250A CN101215536A CN 101215536 A CN101215536 A CN 101215536A CN A200710307250X A CNA200710307250X A CN A200710307250XA CN 200710307250 A CN200710307250 A CN 200710307250A CN 101215536 A CN101215536 A CN 101215536A
- Authority
- CN
- China
- Prior art keywords
- culture
- degradation bacterium
- preparation
- solid
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000015556 catabolic process Effects 0.000 title claims abstract description 37
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 37
- 241000894006 Bacteria Species 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 238000004519 manufacturing process Methods 0.000 title claims description 8
- 239000007787 solid Substances 0.000 claims abstract description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 239000001963 growth medium Substances 0.000 claims abstract description 17
- 239000001888 Peptone Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 16
- 108010080698 Peptones Proteins 0.000 claims abstract description 15
- 235000019319 peptone Nutrition 0.000 claims abstract description 15
- 238000011218 seed culture Methods 0.000 claims abstract description 14
- 238000002156 mixing Methods 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 8
- 239000002609 medium Substances 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 28
- 239000000843 powder Substances 0.000 claims description 16
- 241000209094 Oryza Species 0.000 claims description 14
- 235000007164 Oryza sativa Nutrition 0.000 claims description 14
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 14
- 235000009566 rice Nutrition 0.000 claims description 14
- 235000012054 meals Nutrition 0.000 claims description 12
- 235000015099 wheat brans Nutrition 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 240000005979 Hordeum vulgare Species 0.000 claims description 6
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 6
- 239000002054 inoculum Substances 0.000 claims description 6
- 241000193755 Bacillus cereus Species 0.000 claims description 5
- 241000194108 Bacillus licheniformis Species 0.000 claims description 5
- 241000191973 Staphylococcus xylosus Species 0.000 claims description 5
- 230000003068 static effect Effects 0.000 claims description 4
- 206010013786 Dry skin Diseases 0.000 claims description 3
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 235000013372 meat Nutrition 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 230000010355 oscillation Effects 0.000 claims description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 16
- 238000012258 culturing Methods 0.000 abstract description 5
- 239000003795 chemical substances by application Substances 0.000 abstract description 4
- 238000011081 inoculation Methods 0.000 abstract description 4
- 235000015278 beef Nutrition 0.000 abstract description 2
- 230000002550 fecal effect Effects 0.000 abstract 3
- 238000000227 grinding Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 238000012856 packing Methods 0.000 description 25
- 239000002068 microbial inoculum Substances 0.000 description 20
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 10
- 239000002689 soil Substances 0.000 description 6
- 230000002906 microbiologic effect Effects 0.000 description 5
- 229910052757 nitrogen Inorganic materials 0.000 description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011574 phosphorus Substances 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 239000000945 filler Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000206594 Carnobacterium Species 0.000 description 2
- 241000186145 Corynebacterium ammoniagenes Species 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000002023 wood Substances 0.000 description 2
- 239000004278 EU approved seasoning Substances 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000010903 husk Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Images
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for preparing fecal degradation bacterial agent, which comprises the following steps: inoculating fecal degradation bacteria on the beef extract peptone culture medium culture medium bevel; culturing under the temperature of 32-38 DEG C for 18-24 h to obtain the bevel, inoculating the bevel seed in the liquid seed culture medium, oscillating, aerating and culturing under the temperature of 32-38 DEG C and the rotary speed at 120-200 r/min for 18-24 h to obtain the liquid seed, inoculating the liquid seed in the solid culture medium whose water content is 50-70% under the inoculation quantity of 1-10%, blending evenly, aerating and culturing statically under the temperature at 32-37 DEG C for 48-72 h, finishing culturing when the cell density reaches to 10 <6>-10 <9> g, obtaining the solid culture, drying the solid culture or drying under the temperature at 20-60 DEG C, grinding the dried solid culture into the fecal degradation bacterial agent. The bacterial agent of the invention is very simple in preparation, low in cost, long in preservation and convenient in use.
Description
Technical field
The present invention relates to a kind of production method that is used to exempt from the dejection degradation bacterium preparation of water degraded type bio-toilet.
Background technology
Traditional water is flushed the toilet when using, and not only needs to consume a large amount of wash-down waters, but also produces a large amount of sanitary sewages thereupon.Increase day by day in world population, urbanization and rapid economic development, today that shortage of water resources and pollutent increase severely, the problem day that this water loss in traditional lavatory is big, the product pollutent is many shows outstanding.
Therefore, world many countries carried out successively the lavatory especially the Public toilets water saving, exempt from the research of water and reduction of discharging, thereby produced various types of New-type toilets, exempt from water degraded type bio-toilet and be wherein a kind of.In exempting from water degraded type bio-toilet, under the lavatory special container of directly accepting ight soil, place biologic packing material in the container, and be provided with whipping appts.Biologic packing material is made up of fillers such as the microorganism (dejection degradation bacterium) of the ight soil of can degrading and wood chips, the ight soil that directly falls into when going to toilet mixes mutually by stirring with biologic packing material, dejection degradation flora in the biologic packing material ight soil of just efficiently degrading apace, stink and vast scale are eliminated, decrement enters thing thereby eliminate, its a small amount of residuum is after long-term accumulated, be a kind of high-quality ecological organic fertilizer, the gas that moisture evaporated and degraded produce is discharged through suction fan.This shows and exempt from water degraded type bio-toilet in use, removed the water flushing fully from, and do not had pollutant emission, meet the environmental requirement that consumption reduction reduces discharging.
Exempt from the biologic packing material that uses in the water degraded type bio-toilet, not only facility just the time spent need to add, and need to replenish in good time and change.Situation is prepared biologic packing material so according to the actual requirements, dejection degradation bacterium in the biologic packing material must just be cultivated before the biologic packing material preparation, obviously the dejection degradation bacterium is adopted the existing very inconvenience of existing using method of cultivating, be convenient to storage, transportation, use and reliable quality microbial inoculum and the dejection degradation bacterium cultivated to make in advance, just situation is prepared biologic packing material at any time according to demand.In fact the microorganism that biopurification is used is made the measure that microbial inoculum re-uses in advance, has become a kind of development trend.
Summary of the invention
Purpose of the present invention is for a kind of simple and effective method for preparing dejection degradation bacterium preparation is provided, so that in time prepare biologic packing material, satisfies the needs of exempting from water degraded type bio-toilet.
For reaching goal of the invention the present invention by the following technical solutions:
A kind of dejection degradation bacterium preparation production method, described method is: with dejection degradation bacterium bacterial classification inoculation to the beef-protein medium inclined-plane, cultivate for 32～38 ℃ and got the inclined-plane seed in 18～24 hours, again the inclined-plane seed is seeded to liquid seed culture medium, at 32～38 ℃, 120～200r/min speed oscillation aerated culture got liquid seeds in 18～24 hours, with liquid seeds with 1～10% (V/W, be every 100g solid medium inoculation 1～10ml liquid seeds) inoculum size, be inoculated in the solid medium of water content 50～70% (mass content), behind the mixing, 32～37 ℃ of static aerated culture 48～72 hours, when cell concn reaches 10
6～10
9Finish during/gram to cultivate, obtain solid culture, more described solid culture is dried or 20～60 ℃ of dryings, dried solid culture is pulverized and is dejection degradation bacterium preparation.Described dejection degradation bacterium is the bacterial classification of the existing known ight soil of can degrading.Described beef-protein medium inclined-plane is that common beef-protein medium adds the slant medium that agar is made, the common liquid nutrient medium that contains beef extract-peptone that is applicable to the dejection degradation bacterium of described liquid seed culture medium, described solid medium is the common solid base that is used for substratum and water through mixed substratum, and the solid base in the described solid medium is wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal etc.
Described beef-protein medium consists of: 1～10g/L extractum carnis, and 5～20g/L peptone, 1～10g/L sodium-chlor, 10～30g/L agar, solvent are water, pH7.2～7.4.During concrete the preparation, add 1～10g extractum carnis, 5～20g peptone, 1～10g sodium-chlor, 10～30g agar, transfer pH7.2～7.4 to get final product again according to 1000ml water.
Described liquid seed culture medium consists of: 1～10g/L extractum carnis, and 5～20g/L peptone, 1～10g/L sodium-chlor, solvent are water, pH7.2～7.4.During concrete the preparation, add 1～10g extractum carnis, 5～20g peptone, 1～10g sodium-chlor, transfer pH7.2～7.4 to get final product again according to 1000ml water.
Described solid medium quality group becomes: solid base 30～50% (mass content), surplus is a water, and described solid base is following one or both or two or more mixing with arbitrary proportion (being recommended as the mixing of wherein any two kinds of materials by mass ratio 1: 2～10): wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal.
The preparation method of the described dejection degradation bacterium preparation of recommending, described method comprises following sequential steps:
A, the dejection degradation bacterium is inoculated on the beef-protein medium inclined-plane, under 32～38 ℃ of temperature, cultivated 18～24 hours, get the inclined-plane seed, described beef-protein medium is: 5g/L extractum carnis, 10g/L peptone, 5g/L sodium-chlor, 20g/L agar, solvent are water, pH7.2～7.4;
B, the inclined-plane seed is inoculated in the described liquid seed culture medium, 32～38 ℃ of temperature, ventilation, 120～200r/min shaking culture 18～24 hours gets liquid seeds, and described liquid seed culture medium is: the 5g/L extractum carnis, the 10g/ peptone, 5g/L sodium-chlor, solvent are water, pH7.2～7.4;
C, with liquid seeds with 1～10% (V/W) inoculum size, be inoculated in the solid medium of described 50～70% water content, behind the mixing, under 32～37 ℃, aerated culture 48～72 hours gets solid culture, and described solid medium quality group becomes: solid base 30～50%, surplus is a water, described solid base be following a kind of or wherein two kinds with mixing by mass ratio 1: 2～10: wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal.
D, solid culture is dried or 20～60 ℃ of air seasonings, the drying solid culture, pulverize and be dejection degradation bacterium preparation.
Dejection degradation bacterium bacterial classification of the present invention, can adopt existing known bacterial classification, separating obtained in biologic packing material that can also from exempt from water degraded type bio-toilet, use and the soil sample, be accredited as through the Biolog microbial identification system: 1. staphylococcus xylosus (Staphylococcus xylosus); 2. bacillus cereus (Bacillus cereus); 3. produce ammonia rod bacillus (Corynebacterium ammoniagenes); 4. Bacillus licheniformis (Bacilluslicheniformis); 5. movable meat bacillus (Carnobacterium mobil).When being used to prepare dejection degradation bacterium preparation, the solid culture powder that can use above-mentioned single culture to make through cultivation, also can use above-mentioned multiple bacterial classification after cultivating, to obtain the mixture of solid culture powder respectively, usually adopt the mixture of the solid culture powder of multiple bacterial classification, effect is better.
The invention has the advantages that making is very easy, with low cost.Not only production unit is simpler owing to the especially fairly large solid culture of solid culture, and solid culture cell concentration height, the moisture relative content is few, is convenient to convection drying and makes dried microbial inoculum, dried microbial inoculum is more suitable for longer-term than liquid bacterial agent and stores, and it is also convenient to use.Adopt raw materials such as wide material sources, low-cost wheat bran, rice bran as the solid culture based raw material in addition, culturing process is simple in addition, so production cost is very cheap.
Description of drawings
Fig. 1 microbial inoculum and the variation that contrasts biologic packing material eluant COD;
Fig. 2 microbial inoculum and the variation that contrasts bacterium biologic packing material eluant total nitrogen;
Fig. 3 microbial inoculum and the variation that contrasts biologic packing material eluant total phosphorus;
Fig. 4 microbial inoculum and the variation that contrasts the biologic packing material ash content.
Embodiment
Below with specific embodiment technical scheme of the present invention is described, but protection scope of the present invention is not limited thereto:
Embodiment 1:
Respectively with Bacillus cereus (bacillus cereus, Microbiological Lab of Zhejiang Polytechnical University provides) and two kinds of bacterium of Bacillus licheniformis (Bacillus licheniformis, Microbiological Lab of Zhejiang Polytechnical University provides) make single bacterial classification microbial inoculum by the following method:
The substratum of present embodiment is prepared by following consumption:
Beef-protein medium: 5g extractum carnis, 10g peptone, 5g sodium-chlor, 20g agar, water 1000ml, pH7.2～7.4.
Liquid seed culture medium: 5g extractum carnis, 10g peptone, 5g sodium-chlor, water 1000ml, pH7.2～7.4;
The wheat bran of solid medium: 400g, 600g water mixes.
The bacterial classification of getting above-mentioned dejection degradation bacterium respectively is on the beef-protein medium test tube slant, cultivate for 32～38 ℃ and got the inclined-plane seed in 18～24 hours, the inclined-plane seed is inoculated one respectively and is encircled in the 250mL triangular flask that the 100mL liquid seed culture medium is housed, at 32～38 ℃, aerated culture is 18～24 hours on 120～200 rev/mins the rotary shaker, with the inoculum size of cultured liquid seeds with every bottle of 10ml, be inoculated into respectively in the every bottled 200g of having solid medium triangular flask, after shaking up, 32～38 ℃ of static aerated culture 48～72 hours, cultivate the solid culture that finishes and dry naturally in room temperature respectively, dried solid culture separated pulverizing is each single bacterial classification microbial inoculum.
Respectively with Staphylococcus xylosus (staphylococcus xylosus, Microbiological Lab of Zhejiang Polytechnical University provides), Corynebacterium ammoniagenes (produces ammonia rod bacillus, Microbiological Lab of Zhejiang Polytechnical University provides) and Carnobacterium mobil (movable meat bacillus, Microbiological Lab of Zhejiang Polytechnical University provides) make single bacterial classification microbial inoculum by the following method:
The substratum of present embodiment is prepared by following consumption:
Beef-protein medium: 5g extractum carnis, 10g peptone, 5g sodium-chlor, 20g agar, water 1000ml, pH7.2～7.4.
Liquid seed culture medium: 5g extractum carnis, 10g peptone, 5g sodium-chlor, water 1000ml, pH7.2～7.4;
Solid medium: the 360g wheat bran, the 40g Semen Maydis powder, 600g water mixes.
The bacterial classification of getting above-mentioned dejection degradation bacterium respectively is on the beef-protein medium test tube slant, cultivate for 32～38 ℃ and got the inclined-plane seed in 18～24 hours, the inclined-plane seed is inoculated one respectively and is encircled in the 250mL triangular flask that the 100mL liquid seed culture medium is housed, at 32～38 ℃, aerated culture is 18～24 hours on 120～200 rev/mins the rotary shaker, with the inoculum size of cultured liquid seeds with every bottle of 10ml, be inoculated into respectively in the every bottled 200g of having solid medium triangular flask, after shaking up, 32～38 ℃ of static aerated culture 48～72 hours, cultivate the solid culture that finishes, naturally dry in room temperature respectively, dried solid culture is each single bacterial classification microbial inoculum through suitably pulverizing respectively.
The microbial inoculum result of use:
Result of use for the microbial inoculum clearly produced by the inventive method, get five kinds of single microbial inoculum balanced mix that embodiment 1～2 makes, mix with filler (forming) with the ratio of 0.03% (quality) again by wood chip, Pericarppium arachidis hypogaeae and paddy rice husk etc., be mixed with the microbial inoculum biologic packing material, the water degraded type bio-toilet of exempting from of putting into 30 person-times of scales uses; Adopt the contrast biologic packing material of existing cultural method inoculation to use simultaneously, in contrast at the water degraded type bio-toilet of exempting from of identical scale, condition.Sampling regularly in use, the total percent of ash of chemical oxygen demand (COD), total nitrogen, total phosphorus and the biologic packing material of detection of biological filler eluant to investigate both dejection degradation effects, the results are shown in Figure 1～Fig. 4 respectively.(described biologic packing material eluant was broken up in fruit juice mixer 10 minutes for the 10g biologic packing material adds water, and moisturizing is settled to 1 liter supernatant liquor; Chemical oxygen demand (COD) detects and adopts potassium dichromate process; Total nitrogen detects and adopts the determination of total nitrogen content instrument; Total phosphorus detects and adopts the ammonium molybdate spectrophotometry; Total percent of ash detects the combustion oxidation method that adopts.)
As seen from the figure the microbial inoculum biologic packing material and the contrast biologic packing material in use, it is little that the changing conditions of residual organic, total nitrogen, total phosphorus and total percent of ash all differs.Wherein the chemical oxygen demand of microbial inoculum biologic packing material (COD) rate of growth will be lower than the contrast biologic packing material, illustrates that microbial inoculum biologic packing material residual organic is few, and it is more to degrade.This shows by the microbial inoculum of the inventive method production and compare that result of use is good with former method.
Claims (7)
1. dejection degradation bacterium preparation production method, it is characterized in that described method is: the dejection degradation bacterium is seeded to the beef-protein medium inclined-plane, cultivate for 32～38 ℃ and got the inclined-plane seed in 18～24 hours, again the inclined-plane seed is seeded to liquid seed culture medium, 32～38 ℃, 120～200r/min speed oscillation aerated culture 18～24 hours liquid seeds, with liquid seeds with 1～10% inoculum size, be seeded to the solid medium of water content 50～70%, behind the mixing, 32～37 ℃ of static aerated culture 48～72 hours, when cell concn reaches 10
6～10
9Finish during/gram to cultivate, obtain solid culture, more described solid culture is dried or 20～60 ℃ of dryings, dried solid culture is pulverized and is dejection degradation bacterium preparation.
2. the preparation method of dejection degradation bacterium preparation according to claim 1 is characterized in that described beef-protein medium consists of: 1～10g/L extractum carnis, 5～20g/L peptone, 1～10g/L sodium-chlor, 10～30g/L agar, solvent are water, pH7.2～7.4.
3. the preparation method of dejection degradation bacterium preparation according to claim 1 is characterized in that described liquid seed culture medium consists of: 1～10g/L extractum carnis, and 5～20g/L peptone, 1～10g/L sodium-chlor, solvent are water, pH7.2～7.4.
4. the preparation method of dejection degradation bacterium preparation according to claim 1, it is characterized in that described solid medium quality group becomes: solid base 30～50%, surplus is a water, and described solid base is following one or both or two or more mixing with arbitrary proportion: wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal.
5. as the preparation method of dejection degradation bacterium preparation as described in the claim 4, it is characterized in that described solid base is that following any two kinds of materials make up in 1: 2～10 ratios: wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal.
6. the preparation method of dejection degradation bacterium preparation according to claim 1 is characterized in that described method comprises the steps:
A, the dejection degradation bacterium is inoculated on the beef-protein medium inclined-plane, under 32～38 ℃ of temperature, cultivated 18～24 hours, get the inclined-plane seed, described beef-protein medium is: 5g/L extractum carnis, 10g/L peptone, 5g/L sodium-chlor, 20g/L agar, solvent are water, pH7.2～7.4;
B, the inclined-plane seed is inoculated in the described liquid seed culture medium, 32～38 ℃ of temperature, ventilation, 120～200r/min shaking culture 18～24 hours gets liquid seeds, and described liquid seed culture medium is: the 5g/L extractum carnis, the 10g/L peptone, 5g/L sodium-chlor, solvent are water, pH7.2～7.4;
C, with liquid seeds with 1～10% inoculum size, be inoculated in the solid medium of described 50～70% water content, behind the mixing, under 32～37 ℃, aerated culture 48～72 hours gets solid culture, and described solid medium quality group becomes: solid base 30～50%, surplus is a water, and described solid base is following a kind of or wherein two kinds of mixing with mass ratio 1: 2～10: wheat bran, rice bran, soybean cake powder, wheat-flour, barley meal, Semen Maydis powder or rice meal.
D, solid culture is dried or 20～60 ℃ of ventilation dryings, the drying solid culture, pulverize and be dejection degradation bacterium preparation.
7. as the preparation method of the described dejection degradation bacterium preparation of one of claim 1～6, it is characterized in that described dejection degradation bacterium is one of following: 1. staphylococcus xylosus; 2. bacillus cereus; 3. produce ammonia rod bacillus; 4. Bacillus licheniformis; 5. movable meat bacillus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710307250XA CN101215536A (en) | 2007-12-29 | 2007-12-29 | Method for producing dejection degradation bacterium preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA200710307250XA CN101215536A (en) | 2007-12-29 | 2007-12-29 | Method for producing dejection degradation bacterium preparation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101215536A true CN101215536A (en) | 2008-07-09 |
Family
ID=39622046
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA200710307250XA Pending CN101215536A (en) | 2007-12-29 | 2007-12-29 | Method for producing dejection degradation bacterium preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101215536A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432146A (en) * | 2011-08-25 | 2012-05-02 | 西华大学 | Complex microbial inoculum for efficiently converting poultry culture excrement and preparation method thereof |
| CN102766588A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN103735211A (en) * | 2013-10-21 | 2014-04-23 | 上海市政工程设计研究总院（集团）有限公司 | Water-free bio-toilet |
| CN104531586A (en) * | 2014-12-31 | 2015-04-22 | 广州舒国生物科技有限公司 | Microbial agent for processing carcasses and preparation method and application thereof |
| CN104988094A (en) * | 2015-06-30 | 2015-10-21 | 河南农业大学 | Method for manufacturing quinclorac solid degrading inoculant |
| CN105238711A (en) * | 2015-09-23 | 2016-01-13 | 浙江富春紫光环保股份有限公司 | Preparation method for sewage treatment plant deodorant bacterial agent |
| CN107439259A (en) * | 2017-09-26 | 2017-12-08 | 上海应用技术大学 | A kind of preparation method of potted orchids special active cultivation matrix |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
| CN113046279A (en) * | 2021-05-06 | 2021-06-29 | 杜普利 | Waterless ecological toilet excrement recycling leavening agent and preparation method thereof |
-
2007
- 2007-12-29 CN CNA200710307250XA patent/CN101215536A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102432146A (en) * | 2011-08-25 | 2012-05-02 | 西华大学 | Complex microbial inoculum for efficiently converting poultry culture excrement and preparation method thereof |
| CN102432146B (en) * | 2011-08-25 | 2013-09-04 | 西华大学 | Complex microbial inoculum for efficiently converting poultry culture excrement and preparation method thereof |
| CN102766588A (en) * | 2012-04-13 | 2012-11-07 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN102766588B (en) * | 2012-04-13 | 2014-01-01 | 轻工业环境保护研究所 | Kitchen waste destructive compound microbial bactericide, its preparation method and application thereof |
| CN103735211A (en) * | 2013-10-21 | 2014-04-23 | 上海市政工程设计研究总院（集团）有限公司 | Water-free bio-toilet |
| CN104531586A (en) * | 2014-12-31 | 2015-04-22 | 广州舒国生物科技有限公司 | Microbial agent for processing carcasses and preparation method and application thereof |
| CN104531586B (en) * | 2014-12-31 | 2018-09-14 | 广州舒国生物科技有限公司 | A kind of microbial bacterial agent and its preparation method and application of processing spoil |
| CN104988094A (en) * | 2015-06-30 | 2015-10-21 | 河南农业大学 | Method for manufacturing quinclorac solid degrading inoculant |
| CN105238711A (en) * | 2015-09-23 | 2016-01-13 | 浙江富春紫光环保股份有限公司 | Preparation method for sewage treatment plant deodorant bacterial agent |
| CN107439259A (en) * | 2017-09-26 | 2017-12-08 | 上海应用技术大学 | A kind of preparation method of potted orchids special active cultivation matrix |
| CN112812990A (en) * | 2020-12-31 | 2021-05-18 | 宜兴艾科森生态环卫设备有限公司 | Compound microbial agent for excrement treatment and application and compounding method thereof |
| CN113046279A (en) * | 2021-05-06 | 2021-06-29 | 杜普利 | Waterless ecological toilet excrement recycling leavening agent and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101215536A (en) | Method for producing dejection degradation bacterium preparation | |
| Zhang et al. | Two types nitrogen source supply adjusted interaction patterns of bacterial community to affect humifaction process of rice straw composting | |
| Zhang et al. | Effects of rhamnolipid and initial compost particle size on the two-stage composting of green waste | |
| CN109402015B (en) | Bacillus amylovora and application thereof | |
| CN102887736B (en) | Method for preparing special fertilizer by treating kitchen waste, sludge and domestic waste in one machine | |
| CN102276301B (en) | Straw decomposing agent and production method thereof | |
| CN101333510B (en) | Method for processing sludge and preparing bio organic fertilizer and special leaven thereof | |
| CN104212746B (en) | A kind of changing food waste of salt tolerant processes composite bacteria agent and its preparation method and application | |
| CN101696393A (en) | Compound bacterium agent and method for accelerating fast maturity by inoculating compound bacterium agent to compost | |
| CN104761310A (en) | Ecologic organic fertilizer and preparation method thereof | |
| CN105237294A (en) | Biomass charcoal soil conditioner and preparation method thereof | |
| CN106867545A (en) | A kind of saline-alkali soil restoration agent, preparation method and applications from sludge | |
| CN103695317B (en) | There is the production method of the efficient phosphate-solubilizing penicillium oxalicum microbial inoculum of heavy metal tolerance characteristic | |
| CN108130295B (en) | Microbial organic fertilizer fermentation inoculant | |
| CN104496592A (en) | Organic earthworm cast biological bacterial fertilizer | |
| CN101205527A (en) | Composite microbial agents and production method thereof | |
| Janušauskaitė et al. | Soil mineral nitrogen and microbial parameters as influenced by catch crops and straw management. | |
| CN101914445A (en) | Indigenous probiotic microorganism solid fungicide and preparation method and application thereof | |
| CN105601361A (en) | Method for producing organic fertilizer from chicken manure, straws and traditional Chinese medicine residues | |
| CN101913950A (en) | Organic fertilizer preparation method and multi-strain breeding of biodegradable coal gangue | |
| CN109111256A (en) | Microbial biomass can convert superhigh temperature fermentation process | |
| CN110591966B (en) | Environment deodorization composite microbial inoculant and preparation method thereof | |
| Haiba et al. | Transformation of nutrients and organic matter in vermicomposting of sewage sludge and kitchen wastes | |
| CN101774835A (en) | Composting material composition and composting method | |
| Li et al. | Performance exploration and microbial dynamics of urine diverting composting toilets in rural China |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080709 |