CN101558165B - 高粘度迪优坦胶及其制备方法 - Google Patents
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Abstract
本发明描述了迪优坦多糖的生产，与以前生产的重复单元类型相同的多糖相比，所述多糖显示粘度特性提高。通过产生鞘氨醇单孢菌ATCC53159的衍生物生产这种改进的迪优坦多糖，所述衍生物含有其中克隆了迪优坦多糖的生物合成基因的多拷贝广谱宿主性质粒。所述质粒能在宿主鞘氨醇单孢菌菌株内产生用于这种多糖合成的多个基因拷贝。以这种方式提供的方法不仅能提高靶迪优坦多糖的产量，还能产生物理特性改进(上述较高的粘度)的迪优坦多糖。已证实这种迪优坦多糖在油田应用和水泥材料中作为增粘剂可能特别有用。本发明还包括产生这种改进迪优坦多糖的创造性方法以及这种方法中产生改进迪优坦所需的新克隆基因。此外，本发明包括含有所需DNA序列的工程改造的新鞘氨醇单孢菌菌株。
Description
发明领域
本发明描述了与重复单元类型相同的以前生产的多糖相比，显示粘度特性增加的迪优坦(diutan)多糖。通过产生鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159的衍生物来制备这种改进的迪优坦多糖，所述衍生物含有其中克隆了迪优坦多糖的生物合成基因的多拷贝广谱宿主性质粒。所述质粒能在宿主鞘氨醇单孢菌菌株内产生用于这种多糖合成的多个基因拷贝。以此方式提供的方法不仅能提高靶迪优坦多糖的产量，还能产生物理特性改进(上述较高的粘度)的迪优坦多糖。现已证实这种迪优坦多糖在油田应用和水泥材料中作为增粘剂(viscosifier)可能特别有用。本发明还包括产生这种改进迪优坦多糖的创造性方法以及这种方法中产生改进迪优坦所需的新克隆基因。此外，本发明包括含有所需DNA序列的工程改造的新鞘氨醇单孢菌菌株。
发明背景
多糖或树胶一般用于使水溶液增稠或使之成为凝胶状，通常分为两类：增稠剂和胶凝剂。典型的增稠剂包括：淀粉、黄原胶、迪优坦胶、文莱胶(welangum)、瓜尔胶、羧甲基纤维素、海藻酸盐、甲基纤维素、刺梧桐树胶和黄蓍胶。常见的胶凝剂包括明胶、结冷胶、淀粉、海藻酸盐、果胶、角叉菜聚糖、琼脂和甲基纤维素。
多年来，某些多糖，或者更具体地说，生物胶，例如黄原胶、结冷胶、文莱胶和迪优坦胶通过微生物发酵生产。这些生物胶表现出不同特征，例如使其应用于许多不同领域的粘度改进能力。用于食品，例如糖果凝胶(confectioneryjelly)、果酱和果冻、甜食凝胶、糖霜和乳制品，以及微生物培养基组分的胶凝剂属于该清单。此外，可将增稠剂用于许多最终应用领域以改进靶液体的粘度。特别感兴趣的是这些胶能改变地下和/或水下石油液体粘度，从而有助于收集这些液体，虽然可能有许多其它不同的最终应用存在(例如，包括水泥生产)。已从不同的细菌来源产生了不同的生物胶，例如野油菜黄单孢菌(Xanthomonascampestris)的黄原胶、伊乐藻鞘氨醇单孢菌(Sphingomonas elodea)的结冷胶、鞘氨醇单孢菌ATCC 31555的文莱胶和鞘氨醇单孢菌ATCC 53159的迪优坦胶(S-657)。在过去已对这些菌株进行了遗传修饰以求明显改变通过上述发酵方法产生的胶物质。这种修饰能导致诸如除去酰基等变化，从而产生表现出不同物理特性的不同胶物质。这些遗传修饰的类型一般是：通过改变宿主生物内的基因表达来最终改变靶生物胶的组成，或者通过引入单独显示基因扩增的质粒来提高靶生物胶产量(例如授予Pollock等的美国专利号5,854,034，5,985,623和6,284,516以及授予Pollock个人的美国专利号6,709,845)。
迪优坦胶(也称为杂多糖(heterpolysaccharide)S-657)通过发酵菌株鞘氨醇单孢菌ATCC 53159制备，其在水溶液中表现出增稠、助悬和稳定特性。迪优坦通常表现为六聚体重复单元，所述重复单元由主链中的4个糖(葡萄糖-葡糖醛酸-葡萄糖-鼠李糖)以及与所述葡萄糖残基之一相连的两鼠李糖残基的侧链构成。迪优坦胶的结构细节见Chowdhury，T.A.，B.Lindberg，U.Lindquist和J.Baird，Carbohydrate Research 164(1987)117-122。迪优坦显示每个重复单元具有两个乙酰基取代基，参见Diltz等，Carbohydrate Research 331(2001)265-270。两篇参考文献均通过引用全文纳入本文。制备迪优坦胶的细节可参见通过引用全文纳入本文的美国专利号5,175,278。可采用标准发酵技术，例如利用碳水化合物源(非限制性例子如葡萄糖、麦芽糖等)、氮源和其它盐类从鞘氨醇单孢菌菌株制备迪优坦。
某些工业需要各种野生型迪优坦生物胶赋予的物理特征，特别是其粘度改进特性和/或保水特性。不幸的是，已证实产生迪优坦的成本效率低。此外，目前这些成本问题妨碍了迪优坦的广泛应用，因为这种生物胶所表现出的粘度不足以替代其它更便宜但有效的生物胶(例如，黄原胶)。因此，需要提供至少能以较低成本生产这种有效迪优坦的方法，和/或提供生产物理特性显示明显改进的迪优坦型生物胶的方式。目前，对任何类型的相关结冷胶类多糖(sphingan)生产的唯一描述(对于迪优坦未证明有任何特异性)涉及较高的产率(见上述Pollock等的专利)。现在还没有任何方式讨论或合理地提出能提供某种方法来生产分子量较高的迪优坦胶，借助这种生产方法，所述迪优坦胶在粘度测量值会体现出改善。
发明简述
现已知道，在宿主鞘氨醇单孢菌微生物内扩增迪优坦生物合成所用的某些新分离的DNA序列不仅能提高迪优坦胶产量，还产生了表现出粘度特性增加的迪优坦胶。因此，这种新的DNA序列(通过任何熟知的方法，例如但不限于利用质粒引入宿主微生物内)提供了迪优坦合成方法所寻求的所需结果。采用质粒扩增这些基因的这种方法的明显优点在于将这种分离DNA序列掺入迪优坦合成过程较为简单。另一优点是能产生这种粘度特性较高的靶迪优坦胶，同时还可能提高发酵生产效率(如果需要的话)。
因此，本发明包括在许多不同的粘度测试中表现出改进的迪优坦胶。其中：i)固有粘度高于150，优选高于155，更优选高于160dL/g；ii)海水3rpm粘度高于35，优选高于37，更优选高于40，最优选高于42表盘读数(dial reading)；iii)海水0.3rpm粘度高于35,000，优选高于39,000，更优选高于40,000，最优选高于41,000厘泊(cP)；PEG低剪切率粘度高于3500，优选高于3700，更优选高于3900，最优选高于4000cP。如以上术语所定义的，本发明还包括产生这种迪优坦胶的方法，所述方法通过将特定的基因簇引入宿主鞘氨醇微生物并发酵所述微生物以产生迪优坦胶。此外，本发明包括特定的DNA序列和任何载体(例如质粒)来提供基因的多个拷贝或利用更强的启动子来提高这些基因表达，等等。另外，还包括遗传修饰的鞘氨醇菌株，所述菌株含有这种独特的分离DNA序列所确定的迪优坦生物合成基因的多个拷贝。
发现这种独特的分离DNA序列需要至少一种迪优坦生物合成酶，即DpsG聚合酶。在另一可能的实施方式中，这种迪优坦生物合成酶包括DpsG聚合酶和葡萄糖-1-磷酸胸苷基转移酶；dTDP-6-脱氧-葡萄糖-3-5-差向异构酶；dTDP-D-葡萄糖-4，6-脱水酶；和dTDP-6-脱氧-L-甘露糖-脱氢酶。在还有另一可能的实施方式中，这种迪优坦生物合成酶包括DpsG聚合酶和鼠李糖基转移酶IV；β-1，4-葡糖醛酸基转移酶II；葡糖基-异戊二烯基磷酸转移酶I；和葡糖基转移酶III。在还有另一可能的实施方式中，这种迪优坦生物合成酶包括DpsG聚合酶和多糖输出蛋白DpsD、DpsC和DpsE。在还有另一可能的实施方式中，这种diutab生物合成酶包括鼠李糖基转移酶IV；β-1，4-葡糖醛酸基转移酶II；葡糖基-异戊二烯基磷酸转移酶I；葡糖基转移酶III；葡萄糖-1-磷酸胸苷基转移酶；dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶；dTDP-D-葡萄糖-4，6-脱水酶；和dTDP-6-脱氧-L-甘露糖-脱氢酶。本发明方法和属于本发明产物的迪优坦生物合成酶一般选自：聚合酶；裂合酶；鼠李糖基转移酶IV；β-1，4-葡糖醛酸基转移酶II；葡糖基转移酶III；多糖输出蛋白；分泌蛋白；葡糖基-异戊二烯基磷酸转移酶I；葡萄糖-1-磷酸胸苷基转移酶；dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶；dTDP-D-葡萄糖-4，6-脱水酶；dTDP-6-脱氧-L-甘露糖-脱氢酶和它们的组合。本发明还包括分离的核酸分子(除靶染色体上可能存在的DNA外)，其编码如以下SEQ ID NO所示的至少一种迪优坦生物合成酶：5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41和43；或编码与以下SEQ ID NO有至少95％相同的酶5、7、9、11、13、15、17、19、21、23、25、27、29、31、33、35、37、39、41和43。
因此，本发明方法(以及由此制备的产品)涉及结冷胶类多糖胶，特别是迪优坦型，包括但不限于：S88、S60和S657。
如上所述，本发明是发展的顶点，并且实现了将多拷贝的特定DNA序列引入某些鞘氨醇单孢菌菌株增加高粘度迪优坦多糖的生物合成产量。与非工程改造的细菌相比，含有用于提高产量的这些基因的工程改造细菌产生的迪优坦多糖量明显较高，同时产生了上述的高粘度特性。
按照本发明，可通过本领域熟知的技术分离、回收和克隆引入宿主微生物中的DNA序列(采取任何熟知的形式，例如非限制性的例子还是质粒)，从而产生上述产量增加和粘度增加的特性(不想依赖于任何具体的科学理论，但据信这是经由分子量范围特性增加所致)。然后，将多拷贝的所述DNA递送入鞘氨醇单孢菌属细菌中(借助质粒，其它已知的方式)或借助合适的例如更强启动子提高基因表达。插入靶细菌后，可通过发酵该工程改造细菌并根据所产生的数量和质量来比较产量从而测定迪优坦的生产情况。通过比较本发明方法所得的迪优坦产量和野生型迪优坦生产菌株(ATCC 53159)的可同时测定产量增加和粘度增加(量)。
附图简述
图1图示了迪优坦胶生物合成的分离基因。标出了推定的或已知的基因。还标出了插入不同质粒的区段。
图2图示了利用这种创造性迪优坦生物胶材料实现的固有粘度测量值的改善。
发明详述
与本发明有关的以下术语用于通篇说明书中，其意义如下所示：
通篇说明书利用术语“鞘氨醇单孢菌”指鞘氨醇单孢菌属的革兰氏阴性细菌菌株。
通篇说明书利用术语“提高的生产者”或“提高的生产”描述含有多拷贝DNA序列的工程改造细菌，所述DNA序列分离自与野生型细菌的相同菌株相比产生显著较高(以重量计至少高约5％)迪优坦多糖的相同菌株。
利用术语“分离的”描述从某微生物中取出并经历至少一定程度纯化，即一步或多步纯化步骤的DNA，可利用限制性酶切割所述DNA并将其克隆或插入质粒载体，或者插入或掺入细菌中。
利用术语“序列”描述根据其核苷酸单位鉴定的具体DNA区段。通篇说明书利用术语“插入”描述将从生产迪优坦的鞘氨醇单孢菌菌株的染色体DNA分离的DNA区段转移入鞘氨醇单孢菌菌株的过程和结果(非限制性例子是借助质粒)。首先可采用本领域熟知的技术将这种分离的DNA引入(依然是非限制性的可能性)所需质粒(此处是pLAFR3)，然后通过，例如接合或迁移将其转移入受者鞘氨醇单孢菌细菌。插入受者鞘氨醇单孢菌细菌后，含有相关DNA序列的质粒在受者细胞中复制，从而获得产生高粘度(仍然相信是高分子量范围的)迪优坦多糖所需的DNA区段的若干(至少两个，一般是4-10个)拷贝。采用接合或迁移将所述质粒载体转移入受者细菌通常是有效的。还可利用纯化的DNA以电穿孔或化学转化感受态细胞。可利用其它载体或噬菌体将DNA转移入宿主细胞中。在产生迪优坦的受者鞘氨醇单孢菌中，无需使DNA区段维持在质粒(或其它熟知的递送载体)上。将其它的数拷贝DNA区段引入细菌染色体是惯常的，从而可通过复制细菌DNA的相同机制复制各代区段。或者，可利用更强的启动子元件提高基因表达。
利用术语“基因扩增”表示例如通过将靶基因克隆到多拷贝质粒上(例如，4-10个拷贝)或将基因的多个拷贝(例如，4-10个)插入细菌基因组中来增加基因的拷贝数，或者表示通过修饰启动子元件来增加基因表达。这些方法和其它方法均可增加所编码蛋白质的数量。
通篇说明书利用术语“生物合成”描述通过鞘氨醇单孢菌细菌生物学产生或合成迪优坦。通过许多细菌酶所调控的一系列步骤从单个碳水化合物单元合成迪优坦多糖。
可以任何选择的形式(例如仍然优选质粒形式，但不一定)掺入受者细菌的相关DNA序列编码已知对迪优坦多糖的产量增加和分子量增加有益或必需的遗传信息。此外，不依赖于具体的科学理论，虽然据信本发明具体DNA序列(例如质粒pS8中的)能诱导，而不只是提高产量，但还增加了在迪优坦本身的各聚合物内聚合的重复单元数量。因此，据信重复单元的这种增加使得迪优坦胶提供的粘度特性出乎意料地高。由于检测到固有粘度增加，因而可假定分子量增加，二者有幂定律关系。因此，已知线形聚合物(如迪优坦胶)的固有粘度实际上以那种关系与分子量成比例。
采用标准技术和方法分离相关DNA序列，所述DNA序列是本发明方法的基础并产生了粘度增加的迪优坦多糖。因此，可采用标准方法培养产生迪优坦的鞘氨醇单孢菌菌株，进而从中产生这些序列。然后可通过，例如先将细菌细胞离心并重悬，再经纯化柱洗脱DNA进行DNA提取。纯化完成后，可用限制性核酸内切酶消化分离的DNA，克隆入所需质粒或其它递送载体中，然后转移至受者菌株。可采用本领域已知的其它技术而不作具体限定。
在本发明中，DNA的克隆取决于本领域标准的常规技术和方法。应该注意，可采用任何方法克隆本发明DNA区段，本发明不限于，例如利用质粒克隆载体。例如，可通过插入噬菌体载体来克隆DNA片段。
然后可利用质粒或其它递送载体将克隆的DNA序列引入鞘氨醇单孢菌菌株。然后可通过发酵，利用遗传修饰的鞘氨醇单孢菌菌株产生迪优坦。适于发酵的培养基基本上是水性培养基，其通常含有碳源，例如包括葡萄糖、乳糖、蔗糖、麦芽糖或麦芽糖糊精在内的碳水化合物；氮源，例如无机铵，有机硝酸酯，有机氨基酸或蛋白物质，如水解酵母菌、大豆粉或酪蛋白、蒸馏酒厂废液浓缩物或玉米浆；和无机盐。各种发酵培养基支持产生本发明迪优坦。
发酵肉汤中可含有各种含量的碳水化合物，但以重量计通常在发酵培养基的约1-10％之间(优选2-8％)。可先加入碳水化合物，再发酵，或者可在发酵期间加入。以重量计，水性培养基中的氮含量在约0.01％-约0.4％。可使用一种碳源或氮源，也可使用这些碳氮源的混合物。发酵鞘氨醇单孢菌细菌可用的无机盐是含有钠、钾、铵、硝酸根、钙、磷酸根、硫酸根、氯、碳酸根和相似离子的盐类。还优选包含痕量金属，例如镁、锰、钴、铁、锌、铜、钼、碘化物和硼酸盐。
发酵可在约25℃-40℃之间，优选约27℃-35℃的温度范围进行。可通过体积放大的标准方法制备接种物，包括摇瓶培养和小规模的深层搅拌发酵。制备接种物的培养基可以与生产培养基相同，或者可以是本领域熟知的几种标准培养基中的任一种，例如Luria肉汤或YM培养基。可采用多个种子阶段(seedstage)以获得所需的接种体积。典型的接种体积范围是最终发酵总体积的约0.5％-约10％。
发酵容器可装有搅拌器来搅拌内含物。该容器还可装有自动pH和气泡控制装置。可将生产培养基加入容器中，通过加热来适当灭菌。或者，可先将碳水化合物或碳源单独灭菌再加入容器中。可将经培养的种子培养液加入冷却的培养基中(通常在约27℃-约35℃的优选发酵温度)，搅拌发酵培养液约48-约110小时，从而产生高粘度的肉汤。通过用醇，一般是异丙醇沉淀等标准方法从肉汤中回收迪优坦多糖。
本发明的优选实施方式
包括附图详述
提供以下实施例是为说明本发明。实施例的描述不应误解为以任何方式限制了本发明的范围。
DNA序列分离/质粒产生
为首先分离和测定上述创造性结果的合适序列，如下所示构建ATCC53159微生物的基因文库：分离鞘氨醇单孢菌ATCC 53159的染色体DNA，用Sau3AI限制性核酸内切酶部分消化。用琼脂糖凝胶纯化15-50kb范围内的DNA片段，将这些片段连接入BamHI消化的粘粒克隆载体pLAFR3(根据Staskawicz等，“分子表征丁香假单胞菌大豆致病变种群0和群1的克隆无毒力基因”(Molecular characterization of cloned avirulence genes from race 0and race 1 of Pseudomonas syrinae pv.Glycinea)，J.Bacteriology.1987.169：5789-94)，该载体分离自大肠杆菌(Eschericia coli)菌株JZ279(Harding等，“在野油菜黄单孢菌中生物合成黄原胶必需的基因簇的遗传学和物理分析”(Genetic and physical analysis of a cluster of genes essential for xanthangum biosynthesis in Xanthomonas campestris)，J.Bacteriology.1987.169：2854-61)。将连接反应(产物)包装入λ噬菌体颗粒(利用吉格帕克III金包装提取物(Gigapack III Gold packaging extract)，斯特拉基因公司(Stratagene)，拉霍亚，加利福尼亚州)，并转染入文库效率(Library Efficiency)大肠杆菌DH5αMCR细胞中(生命技术公司(Life Technologies)，罗克维尔，马里兰州)。合并约10,000个四环素耐受性菌落以形成基因文库。然后分离该文库的各序列。该实施例所进行的这项工作包括从鞘氨醇单孢菌ATCC53159微生物分离用于多糖生物合成的特定基因。
通过与多糖合成缺陷型突变体，特别是第一个步骤，即葡糖基转移酶I被阻断的那些突变体互补来鉴定用于多糖生物合成的这些基因。由于最初未获得ATCC 53159的转移酶I缺陷型突变体，利用与伊乐藻鞘氨醇单孢菌和野油菜黄单孢菌的转移酶I缺陷型突变体互补来鉴定用于迪优坦多糖生物合成的基因。可利用提供IncP转移功能的辅助质粒，通过三-亲本接合(tri-parentalconjugation)将质粒pLAFR3从其大肠杆菌宿主转移至其它革兰氏阴性细菌(根据Ditta等，“革兰氏阴性细菌的广谱宿主性DNA克隆系统：构建苜蓿根瘤菌的基因库”(Broad host range DNA cloning system for gram-negativebacteria：construction of a gene bank of Rhizobium meliloti)，Proc.Natl.Acad.Sci.1980.77：7347-51.)。大肠杆菌中每个染色体的RK2型质粒拷贝数估计有5-7个(Figurski等，“利用体外构建的杂交质粒中的Inc P-I质粒RK2抑制ColE1复制特性”(Suppression of CoIE1 replication properties by the Inc P-Iplasmid RK2 in hybrid plasmids constructed in vitro)，J.MoI.Biol.1979133：295-318.)。
通过三亲本接合将大肠杆菌中的ATCC 53159染色体DNA的基因文库转移入伊乐藻鞘氨醇单孢菌ATCC 31461的非黏液突变体(nonmucoid mutant)(GPS2)，选择四环素和氯霉素耐受性。所用的辅助质粒是pRK2013(大肠杆菌菌株JZ279中)，其含有窄宿主性复制起点，但显示出迁移pLAFR3所需的反式作用功能(trans acting function)。质粒pRK2013在鞘氨醇单孢菌菌株中不复制。伊乐藻鞘氨醇单孢菌ATCC 31461产生多糖结冷胶。结冷胶和迪优坦多糖均具有由[→4)-α-L-鼠李糖-(1→3)-β-D-葡萄糖-(1→4)-β-D-葡糖醛酸-(1→4)-β-D-葡萄糖-(1→]构成的相同四糖重复单元。然而，迪优坦还包含与所述葡萄糖残基之一相连的两个鼠李糖分子构成的侧链，该侧链经乙酰基修饰，而结冷胶不具有侧链糖并经乙酰基和甘油基修饰。突变型GPS2在多糖生物合成的第一步中有缺陷，即通过葡糖基转移酶I将葡萄糖-1-磷酸从UDP-D-葡萄糖转移至细菌异戊二烯基(bactoprenyl)磷酸脂质载体。以非黏液菌落为背景，从四环素选择平板分离产生多糖(黏液)的菌落。推测恢复产生多糖的克隆包含编码葡糖基转移酶I的ATCC 53159基因加上约20-25kb毗连DNA。从8个黏液GPS2转接合子分离质粒DNA，通过电穿孔转移至大肠杆菌菌株DH5α(生命技术公司)。从大肠杆菌分离质粒以而获得用于限制性核酸内切酶HindIII/EcoRI(切割多接头中BamHI限制性核酸内切酶位点的任一侧)进行双重消化的足够DNA，进而从载体上切下插入的DNA。通过凝胶电泳测定克隆中插入DNA的大小。通过从侧接载体的BamHI位点的质粒序列的特异性引物开始测序来测定几种质粒的终序列。利用BLASTX比较计算机数据库中的序列来分析各序列。这些质粒中的两个，pS8和pS6示于图1。类似地，通过三亲本接合将ATCC 53159基因文库转移入转移酶I缺陷的利福平耐药性非黏液野油菜黄单孢菌突变体(CXC109)中(例如，上述Harding等的参考文献所述)，选择四环素和利福平耐药性。野油菜黄单孢菌产生黄原胶多糖，转移酶I将葡萄糖-1-磷酸从UDP-D-葡萄糖转移至细菌异戊二烯基磷酸脂质载体也能启动其合成(Ielpi等，“在野油菜黄单孢菌中顺序装配和聚合黄原胶多糖的聚异戊烯醇连接的五糖重复单元”(Sequential assembly andpolymerization of the polyprenol-linked pentasaccharide repeating unit of thexanthan polysaccharide in Xanthomonas campestris)，J.Bacteriology.1993.175：2490-500)。如上所述从黏液转接合子纯化质粒并如上所述测定末端序列。图1示出了这些质粒中的两种，pX6和pX4。
德克萨斯州休斯顿市的拉克技术公司(Lark Technologies Inc.，Houston，TX)采用双链鸟枪测序对克隆在质粒pS8和pX6中的S657 DNA进行了完全测序。分析了这些序列以鉴定用于迪优坦生物合成的基因(如图1所示)。根据与数据库中其它基因，特别用于S-88结冷胶类多糖(例如，上述‘516Pollock等的专利所述的)、GeneBank登录号U51197和结冷胶(GenBank AY217008和AY220099)生物合成的公布基因的同源性指定基因功能。鉴定了编码主链中四种糖的转移酶的基因和用于dTDP-鼠李糖合成的4种基因。用于多糖分泌的基因基于与生物合成其它多糖的基因的同源性。两种基因编码与参与蛋白质分泌的蛋白质同源的蛋白质。推测两种基因编码聚合酶和裂合酶。质粒pX6中的插入物含有17种基因，包括编码转移酶I(启动迪优坦合成的第一步)的基因dpsB，用于分泌的基因和用于dTDP-鼠李糖合成的4种基因，但缺乏转移酶II、III和IV的基因，和聚合酶及裂合酶的推测基因。质粒pS8含有dps基因簇的20种基因，包括所有4种主链糖转移酶的基因，用于dTDP-鼠李糖合成的四种基因，用于多糖分泌的基因，包括聚合酶和连接酶的推测基因，但缺乏功能未知的基因，orf6和orf7。质粒pS6含有用于分泌和4种糖转移酶的基因，但不含用于dTDP-鼠李糖合成的所有基因或聚合酶的基因。质粒pX4只含有dps区域的一小部分，但包含Pollock等报道的用于dTDP-鼠李糖合成的4种基因和编码转移酶I的基因，从而足以导致鞘氨醇菌株中多糖产量增加。
菌株产生
然后通过上述的三亲本接合将上述四种质粒引入鞘氨醇单孢菌ATCC53159号菌株中，从而形成新的S657工程改造菌株(S657/pS8、S657/pS6、S657/pX6和S657/pX4)。然后如上所述进行发酵，从而产生了下述的生物胶物质。所有4种质粒对迪优坦生产量均产生有益作用；然而，pS8质粒还出乎意料地极大提高了迪优坦粘度并增加了其分子量。提供了pS8的DNA序列(26278bp)(1号DNA序列)，下表1和图1中以表格的形式列出了编码的基因。质粒pS8中的插入物DNA包含基因DpsG到rmlD和基因dpsS和orf7的一部分。
以下基因列表基本上是图1所示质粒pS8中插入物的DNA序列所代表基因的清单。
表1
ps8质粒插入物上的基因
起始 终止 名称 描述
2* 1054 dpsS (部分)与gelS同源
2738 1113C dpsG 推测的聚合酶
4895 2898C dpsR 推测的裂合酶
5093 6031 dpsQ 推测的鼠李糖基转移酶IV
7082 6111C dpsl 未知
7121 8167 dpsK β-1，4-葡糖醛酸基转移酶II
8164 9030 dpsL 葡糖基转移酶III
10467 9079 C dpsJ 未知
11076 12374 dpsF 未知
12389 13306 dpsD 推测的多糖输出蛋白
13341 14687 dpsC 推测的多糖输出蛋白
14687 15394 dpsE 推测的多糖输出蛋白
15405 16286 dpsM 推测的多糖输出蛋白
16270 16968 dpsN 推测的多糖输出蛋白
18454 17060 C atrD 推测的分泌蛋白
20637 18451 C atrB 推测的分泌蛋白
21229 22641 dpsB 葡糖基-异戊二烯基磷酸转移酶I
22757 23635 rmlA 葡萄糖-1-磷酸胸苷基转移酶
23632 24198 rmlC dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶
24202 25263 rmlB dTDP-D-葡萄糖-4，6-脱水酶
25263 26129 rmlD dTDP-6-脱氧-L-甘露糖-脱氢酶
26277* 26146C orf7 (部分)功能未知
*第一框内密码子，起始密码子不存在
迪优坦产生
在Applikon 20L发酵罐中用相同的液体培养基进行三次发酵，同时搅拌并通气，以测定含质粒的工程改造鞘氨醇单孢菌S657菌株相对于不含质粒的S657野生型菌株的dioutan产量。对于含质粒的菌株，在整个发酵过程中加入5mg/L的抗生素四环素以确保质粒的维持。视需要加入KOH以控制pH。采用两种子阶段，接种转移量为1％-6％。用于发酵的培养基含有玉米糖浆作为碳水化合物来源、可同化的氮源和盐。本领域熟知可用于发酵的营养物，包括碳水化合物，例如葡萄糖、蔗糖、麦芽糖或麦芽糊精；氮源，例如无机氮，如铵或硝酸盐，有机氮，如氨基酸、水解酵母提取物、大豆蛋白或玉米浆；和含有，例如氯、磷酸根、磷酸根、钙、铜、铁、镁、钾、钠或锌的其它盐。
测定肉汤的粘度和沉淀的纤维来检测所得的迪优坦产量。利用布氏粘度计，4号纺锤，60rpm检测发酵肉汤的粘度，结果见表2。发酵结束时，通过熟知的引入葡萄糖淀粉酶的方法处理肉汤来水解任何残留的玉米糖浆寡糖。然后用两体积的异丙醇沉淀肉汤试样中产生的迪优坦胶。用过滤器收集纤维，干燥。在表2中，术语DWY表示水解剩余的玉米糖浆寡糖后可沉淀生物胶的总干重得率。
携带迪优坦生物合成基因额外拷贝的质粒pX4、pX6、pS6或pS8的所得物质产率明显较高。然而，与干重得率增加相比，pS8质粒的肉汤粘度出乎意料地大大增加，表明除增加迪优坦产量外的某些因素影响了粘度。
表2
发酵含质粒的菌株
肉汤粘度
上述四种质粒的任一种所得物质的产量明显较高，而pS8和pS6质粒的肉汤粘度出乎意料地高，因此还表明产物质量高。然后测定了所得迪优坦胶产物的质量，即粘度。
应用测试中迪优坦的流变学
然后分析了这些迪优坦胶样品在两个不同领域的的潜在有益用途：用于原油回收的油田添加剂和用于保水性和快速配制的水泥添加剂。
油田工业依赖于称为“海水粘度”(SWV)的测试来估计原油回收用胶的可接受性能。这种测试基本上是胶增加海水粘度(例如，从海床重复回收)的有效性指标。
通常接受根据测试海水制剂的粘度改进作用来预测得到的胶作为原油回收目的的合适粘度调节剂的可行性。将419.53克海盐(ASTM D-1141-52)混合在9800克去离子水中来制备这种“合成海水”制剂。对于海水粘度测试，将0.86克样品胶加入307.0g合成海水，用泛氏混合机(Fann Multimixer)(9B5型，零件号N5020)以约11,500rpm混合35分钟。35分钟结束时，先将溶液冷却至约26℃，再检测粘度。对于3-rpm读数，将样品置于泛氏样品台(泛氏，35A型；扭簧MOC 34/35F0.2b；摆锤(Bob)B1；转子R1)，将电动机调至低速并将变速器设置在中间位置从而将转速设置为3rpm。然后使读数稳定，从仪表读出剪切应力值，记录为SWV 3rpm表盘读数(DR)。对于0.3-rpm读数，利用布氏粘度计(布氏LV DV-II或DV-II粘度计，装有LV-2C纺锤)检测粘度。纺锤的速度设置为0.3rpm，先使纺锤旋转至少6分钟再将粘度记录为SWV-0.3rpm读数并以厘泊(cP)表示。对于水泥应用，PEG LSRV测试(如下所述，利用聚乙二醇作为分散剂的低剪切率粘度)为该工业提供了粘度添加剂的性能效力指标。这种测试检测了0.25％的生物胶标准自来水(STW)溶液的粘度。将10.0克NaCl和1.47克CaCl2·2H2O加入10升去离子水中来制备STW。对于粘度检测，将0.75克生物胶加入400-mL烧杯中的4.5克聚乙二醇200(CAS 25322-68-3)中，彻底分散。然后，将299克STW加入该烧杯，利用低调(low-pitched)搅拌桨型搅拌器以800±20rpm混合约4小时。混合4小时后，将烧杯置于25℃水浴中，静置约30分钟。然后，利用装有2.5+扭矩弹簧的布氏LV粘度计(或相当的仪器，例如DEV 2.5+型)，以3rpm，使用LV 1纺锤检测粘度，先使纺锤旋转3分钟再检测，以厘泊(cP)表示。
以此方式测试以上产生的迪优坦样品，结果如下所示：
表3
含质粒菌株的迪优坦的流变学
SWV＝海水中的粘度
LSRV＝低剪切率粘度
出乎意料的是，一些含质粒的工程改造菌株产生的本发明迪优坦胶表现出粘度增加有限。然而，最出乎意料的是，pS8菌株的3rpm SWV粘度增加是80％，而对pX6菌株所作的相同分析只比野生型结果高9.6％。质粒pS6和pX4没有明显增加。类似地，较低的SWV rpm测试显示pS8型高于野生型51.5％，而pX6只高2％。最后，聚乙二醇LSRV测试显示与野生型胶相比，pS8造成粘度增加超过77％，而pX6迪优坦增加不到16％，pX4增加7.2％，质粒pS6没有显著增加。这些情况中极其出乎意料的结果再次显示通过在pS8质粒中利用示例的所需基因序列，例如将这种序列引入产迪优坦的靶细菌中的一种方式，(粘度的)极大改善与迪优坦胶产量一致。
因此，通过引入pS8产生的本发明迪优坦显示惊人地提高了所有三种计数的粘度检测值，特别是与野生型和pX6质粒产生的种类相比。因此，估计这种新的迪优坦能在典型的油田条件和水泥应用中起到非常好的作用。
流变学改善的基础解释
以上实施例显示S67/pS8菌株的迪优坦的流变学参数显著增加。因此，海水和PEG低剪切率粘度测量值的这种实质性增加不能单单归因于产量增加，因为pX6菌株也显示了相同的产率结果(如果不大于的话)。实际上，在表2所示的以上实施例中，S657/pS8菌株的干重产率(醇可沉淀的物质)增加了8％，而流变学参数增加得更为显著(52-80％)。进行了基础研究来解释为何菌株S657/pS8获得的流变学改善胜过野生型菌株。
固有粘度是聚合物科学中推测大分子分子量的熟知技术(C.Tanford，1961.《大分子的物理化学》(Physical Chemistry of Macromolecules)约翰威利父子公司(John Wiley&Sons，纽约)。通过绘制比浓粘度(粘度对浓度标准化)与溶液浓度的关系图，并将数据的线性回归外推至0浓度可获得固有粘度(图中的y-截距)。如下表所示，所得的胶出乎意料地显示固有粘度增加。
评估了五种迪优坦样品的固有粘度、中性糖并作了有机酸分析，其中两种来自野生型菌株(对照1，对照2)，三种来自S657/pS8菌株(样品1、样品2、样品3)。通过以下步骤纯化这些样品：醇沉淀、再水合、用次氯酸盐处理、用葡萄糖淀粉酶处理、用溶菌酶处理并最终用蛋白酶处理(按照该顺序)。然后按照4∶1的CBM∶肉汤之比回收迪优坦，干燥并研磨。CBM是以重量计包含约82％异丙醇的异丙醇/水共沸混合物。
如下所示测试各样品的水分含量：通常采用Mettler HB 43卤素水分平衡(halogen moisture balance)测试两份0.7克样品试样。然后求出两次试验结果的平均值，利用这些结果校正水分。
获得水分数据后，根据校正的水分用0.01M NaCl制备0.2％的胶溶液。对于这些试验，制备总共200克0.2％溶液。在分析天平上称取最接近千分之十的胶，将其加入最接近千分之一称取的水中。在400ml高型烧杯(tall formbeaker)中用2.5英寸直径的搅拌桨混合器以1000rpm搅拌各样品两小时。
最初的水合后，用0.01M NaCl将各样品稀释至0.02％。称取20克所述0.2％溶液加入400ml烧杯中，然后加入180ml该稀释剂实现所述稀释。将稀释的样品再混合30分钟。最后从该样品制备用于测定固有粘度的最终稀释液。评估以下浓度的各迪优坦样品：0.004％、0.008％、0.010％和0.012％。
利用
VE系统检测粘度。先用水将Vilastic校正到误差低于2.0％，再进行检测。采用2Hz计时器程序、应力为1和剪切率约121/秒检测各样品，所有检测均在23℃进行。各样品检测5次并求出平均值。然后，利用平均粘度数据计算固有粘度。以下图2和表4提供了这些试验的最终结果。
表4
根据固有粘度计算值比较迪优坦
迪优坦 检测的 固有
样品 固体 粘度
S657对照1 93.76 138.3
S657对照2 92.42 143
S657/pS8样品1 91.7 170.7
S657/pS8样品2 91.4 162.2
S657/pS8样品3 91.94 162.8
这些结果表明S657/pS8菌株一致地产生固有粘度显著较高的迪优坦；事实上，本发明菌株的平均比浓粘度是165.2，而对照是140.7，检测的固体水平均相似。这些结果表明S657/pS8产生的迪优坦的分子量高于野生型对照。
图2显示了在对照和本发明菌株之间固体含量相似时检测到的固有粘度一致较高的这些趋势。
为测定S657/pS8的较高粘度迪优坦胶是否与野生型菌株的迪优坦组成相同，通过测试中性糖和有机酸来确定组成。中性糖分析利用固有粘度检测所用的纯化样品。用三氟乙酸将各纯化样品的等份试样水解(100℃/约18小时)成组分糖。通过高效阴离子交换层析，利用脉冲电流计检测来定量测定水解产物中性糖。通过高效离子交换层析，利用化学抑制电导率检测来定量测定水解产物有机酸。表5总结了中性糖分析的结果。如其所示，S657/pS8菌株的中性糖分布与S657野生型菌株的中性糖分布几乎相同。虽然两种结果不同于理论值，但这些结果表明利用pS8产生的迪优坦胶的重复单元的结构与野生型产生的相同，pS8物质导致粘度有任何增加是因为链较长，即分子量较高。
表5
pS8和野生型(对照)迪优坦菌株的中性糖和有机酸分析
| 菌株 | 鼠李糖％ | 葡萄糖％ | 乙酸盐％ | |
| 样品1 | S657/pS8 | 32 | 19 | 8.9 |
| 样品2 | S657/pS8 | 32 | 19 | 8.2 |
| 样品3 | S657/pS8 | 32 | 17 | 8.6 |
| 对照1 | S657野生型 | 30 | 18 | 8.6 |
| 对照1 | S657野生型 | 33 | 20 | 8.7 |
| 平均值 | S657/pS8 | 32 | 18.3 | 8.6 |
| 平均值 | S657野生型 | 31.5 | 19 | 8.65 |
| 理论值 | --- | 46 | 30 | 8 |
因此，S657/pS8工程改造菌株所产生迪优坦的海水粘度和PEG低剪切率粘度的极大增加是因为迪优坦分子的分子量或长度增加，即每个分子中重复单元更多，而不是因为其组成改变，因此不是因为重复结构本身的改变。流变学(参数)的这种改善也不是单纯因为迪优坦产量增加。虽然对克隆有迪优坦生物合成基因簇不同部分的4种质粒pS6、pS8、pX4和pX6的评估显示生产量均有所增加，但只有质粒pS8显示回收的迪优坦产物的流变学参数有出乎意料且非常高的增加。
对克隆在测试质粒中的迪优坦生物合成基因进行比较提示，最有可能导致分子量增加的基因是基因dpsG，因为该基因存在于pS8中而不存在于其它质粒中。基因dpsG编码与参与多糖合成的其它膜蛋白同源性强的疏水性膜蛋白。该蛋白质的一部分与催化重复单元连接以形成高分子量多糖的聚合酶蛋白具有同源性。已有人推测S60中的同源基因gelG起到结冷胶合成聚合酶的功能(Harding，N.E.等2004.“组织伊乐藻鞘氨醇单孢菌ATCC31461中结冷胶多糖生物合成所需的基因”(Organization of genes required for gellanpolysaccharide biosynthesis in Sphingomonas elodea ATCC31461)J.Ind.Microbiol.Biotech.31：70-82。Sa-Correia，I.等2002.“在少动鞘氨醇单孢菌ATCC 31461中生物合成结冷胶：基因、酶和表多糖产生工程改造”(Gellangum biosynthesis in Sphingomonas paucimobilis ATCC 31461：Genes，enzymes and exopolysaccharide production engineering)J.Ind.Microbiol.Biotechnol.29：170-176)。还从产生多糖S88和S7的鞘氨醇单孢菌菌株ATCC31554和ATCC 21423分离了dpsG的同源物(Pollock等，美国专利号5,854,034，5,985,623和6,284,516；Pollock，T.J.美国专利号6,709,845)。因此，聚合酶基因的额外拷贝很可能对迪优坦分子的分子长度增加有影响。也不能排除dpsG可能需要与迪优坦生物合成基因簇中的其它基因组合以实现观察到的粘度增加。可能的候选对象是编码糖转移酶I、II、III和IV的基因dpsB、dpsL、dpsK和dpsQ，特别是编码将重复单元的第一个糖加入脂质载体的转移酶I的基因dpsB。其它重要的基因可以是dpsD、dpsC和dpsE，它们与基因gumB和gumC同源，而后二者在多拷贝质粒上扩增时显示能增加黄原胶分子量。实现粘度极大增加可能需要克隆在质粒pS8中所有的基因。
虽然联系某些优选实施方式和实践描述和揭示了本发明，但绝非要将本发明局限于那些具体的实施方式，而应涵盖所附权利要求书及其等价方式的范围所限定的结构等价物和所有其它的实施方式及改进。
保藏
根据国际承认用于专利程序的微生物保存布达佩斯条约，以下细菌菌株于2005年10月21日由美国模式培养物保藏所(弗吉尼亚州，马纳萨斯，大学大道10801，20110)的专利保藏部门(Patent Depository)保藏：
含质粒pS8的鞘氨醇单孢菌菌株S657。
序列表
<110>N.哈丁(Harding.Nancy)
Y.帕特尔(patel.Yamini)
<120>高粘度迪优坦胶
<130>05-506，05-506A
<160>45
<170>FastSEQ for Windowes Version 4.0
<210>1
<211>26278
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<400>1
gatcaacggc gccttgctcg gacggcacaa attcgtcctg gtcaatgtgt ccacggtcgc
60
ctcttcgata ctgttccagc tgttcccgct tgtcgtcgcc tggatgatcg gcccggacct
120
gcgaacgctg ctgatcgccg cgctcgttgg ccgggcggtt ccgatgatcg gcatgctgcc
180
cgcgctgtat cgaaaccttt tgcgcggcaa cacgccgcgt tttcacgcca gcgaggcgcg
240
cttcctgata ggctatggcg ggtgggcctc gctcacgacc gtggtagcga ccgtgctcat
300
gatggcggac cgcttcctga ttggcgcact tcttgggccc gtcgccgtga ccatctacac
360
ggcccccctg caactcgcac agcgcgtatc gctgctgccc tccgcactgt ccgccgcgct
420
gttcccgcgc ctgcccagcg cgacgccggc ggagcgcatg gcgcttcaga tccgctcgct
480
gtcgctgatc atgggcggcc ttaccgggat gatcggcggc ggactattgc tggccgcgcc
540
gtttctcgat ctctggatcg gcaagtcgct cggccatgcg ggaacgccgg tcgcgctctt
600
cctgttcttc ggcgcatggt ggaatgcgct ggcgatcatt tcgttcagcg gcctgcaggc
660
gagcggacgg ccgaaagcga gcgcgatcgt ccagggggca gagctgctac ccgtgttgat
720
cgcgctgtat gcagggatcc gatggggcgg cgtgaccggc gccgcagcgg tctttctggg
780
acgctccgcc ctggatttcg tcctgttgac ctggcaggca ggcctgctcc gccagacggt
840
gaagcaagta tccgtatgcg gcgccgttct caccgtcgcg atgctcgtgg gcgcgaccta
900
tcgctattcg gtgccgctct ggtgcgtact cagcgcctgc tgcctggtcg cgctggcagc
960
ctgctcctgg tggacattgg cgcgccagga caaggcactg ctgattggac gattgagccg
1020
aattctacca aagcagcggc aactcgacct atagcctttc cgcaatgcac cgatggacca
1080
caccaacccg ttttaattga cacacacaaa tgctacaccg acaaagacac aggccgagag
1140
cgatatagaa gcgctatgcc tagccccagc gtcataaaga tgaacgggtc attgtcacct
1200
tgcgacagga ctgaccgcgt atttaaaaga acagccagga aagttgctac ggcgagctca
1260
agcgggtagc catctccgct catcttaaga ccacgaaacg cgagcaaaat cattaacgta
1320
atcatcgtgc cgtatagcga aacaaaaccc agcaagccgt aatcagccgc tacggacagg
1380
aaaccactgt cgatcgatag gaagccttgc tgattacgcc acccgacagc gccagcaccc
1440
tctcccgggc catagccgaa gaaagggcgg cgagcgatgg caggcacgcc caagcgaaac
1500
tgctcctgcc tgccttgatt gctaagttga gaagcgcctc caccgagaac acggttgtgg
1560
acggcaggca cgaacatgac cgccagcgac agcgccacca tcaaggcggg atacgtcaac
1620
gtcagcgaaa tgccgacaag cccgcccttt gtggtccgcc accgccgaat tgcccaaata
1680
agcaaataca cggtatgcgc caccaatccc cccaccattg ccagtcgaga accgctaaga
1740
aatccggacg caactacaag aaaatcgaag aaaatccaaa atgccaatct ccctacgcca
1800
cgggaattcg ctatacggtg cagcacgaaa ggaatcgtca aagccgtcaa ctctccccag
1860
acaagcggac tgctgaaagt cgtcaaaacg cggtaagtac cccggaaacc gggcgtaagc
1920
actacggtaa gaaactgctc atcaacgcgc aggaagctcg gaatcgagta ggcccagagg
1980
acgtgcttca cccggaactc cagcacgcca atcgccatca gcacgcccac gcaccaaaac
2040
aagcgcgtaa cccaccactc cggggtgcgc gtgtcggtcc cgatcagcca tagcgagatg
2100
aatgccatcg gcgtcaccgt cagaacgatg ccaatcaacc gcggaattgt ttgcgaggcc
2160
gctggggtcg caatggaggc gacgatctgg accataatga aggcaagcaa tagtcgcgat
2220
gggatcggcg ccgcccgcat aatcgccgcc atctcggatc gaaacttttt cgagaccgaa
2280
agcgagatca tgagcgtgag caatgcgatc gaaccgatca tccgcctgat cgagatccaa
2340
ggcaaaccac caacgctgag cgcaagatag ttcggccaca cgagcgccgc caccatatag
2400
gcgaggtata gttttgccag caggcgagta ggcgcctgcc gcgcctcggg tagcgcccag
2460
atcactacga gcgccatcag aacgaggggc acggccggga tcgccagcat ctggagcggc
2520
agaactgcgg cgagcaggcc gtagactgcg gcaagaaaca tcacgctgac cagcagaacg
2580
gtacgccgcg ccgcgatcgt cacgcctgat cgctcggctt tgtagacggg cagtaccggg
2640
atcgctggct ttgtcagaaa ccgaaccagt cgcaacctgc gaagccgctg catcgctccg
2700
tggaaggccg ggcgacgaaa cgccgaggta gtcgtcatct gcaagtcccc aacaagtccc
2760
caagaggcgc tgccgctcgc atgatcgaag ggttcgcgaa aagcaaggtc gatacgccgc
2820
actccctgcg atgtgccgcc ggatcgcagg agggcacggg cggcgccggc gcaaggccgc
2880
tcaccgcccg cccccgctca ggcgcggtac aggttgtact gatccgccgt agcgctcagt
2940
gtcgccgcgc tgcggattgc gcccatcgcc cccgcggtca tcatgtcgac accgatcttg
3000
ctgacgagcg cgatctgcga ggacgcggca gtacctatag acagcgtact gcccaccgtg
3060
gccaccgtcg caagcggcgt tgccgtgcta gcggcgccgg cacccgccag cagcgcagcg
3120
gcctgcgcgg ccgcgccggt gacgaggctg tccttgaccg tcgccgccgc gctggcgctc
3180
gacgcggtca ccagcgcctg cacctgggcg gcgctgatcg cgccatcgcg gatctcgatg
3240
tcgccgaccg ttccgctgaa tgcggtcgag aacgggctgc cgacatacag cccccaggat
3300
tcggcgggcc gggtcgtgcc ggtcatcgtc gctgtgccgc gttgcatgcc gtctacgtac
3360
agaatcgcgg ttttccgcgt gctgtcgtag gtcagggcga tcttgtgtgt ggcagcatca
3420
agcagcttgg cgccgctcgt caccattgtc tggctgacgc ccgcggcgtt gcgcatggtg
3480
aagctcagtt ccccattggc ctgcagcgaa accgaccagc tctggaagat gccaagaatt
3540
tgcccggccg tggccgtagc cgagtcccgc ttgaggtcga agctgagcgt gaacgccgac
3600
aatgcgtaaa tctgccgcga atagctccgg tttagttcca cccccgtgcc cgtcgagacg
3660
tggaaggcgc tgcccacgac cgccgacacg tccaccgcct ttgtcgtctg gccggtattc
3720
cagtgcgaaa ggtccacgac gccgctgttg ctgaacgaca gatcgagcag cagcgacgga
3780
tttgccgcct tcgcagtcga cagttcggta gtcacctgag cggcagcagc gctcgacacg
3840
ggcggctggt acccgacgcc gggaacgatc aaatcgctga gccgcgccgt agccccatcg
3900
ttgaggccat agatcttgcg gatcgttgcc gagtcactcg tcagcgtacg attgcctgtc
3960
tgcacgatat tgctcgagga gcttgtgacg gtgatcaggt ccgcaacatt gttcttgatc
4020
gtcgcgccat tggttttgtc gaggcgaatc caaaatgatg tgccatccac ttgcgatatc
4080
acgctattgg attcgatatt gacattaacg ccgttaacaa cgttgatacc gtggtaataa
4140
ccattcagat agataagatt gtttttgatg tttacattga catagggaag attaccggcc
4200
tcgtcattca tgaaaatccc ttgcgcgcca gagcccgcgc cctgcatgat gacgttattg
4260
gagatggtga tgttggtatt gcccttgacc ttgcccgccg tgaagaactg aatggcgtcg
4320
ggatgttcgg tgcccacggg aaacaggttc gtgaacgaat ttccgtcgat gacaagattg
4380
ttcatctcag tgaagttcgt atgatcgcgc cggttgtcgt ggaagctgct gttctggacc
4440
accatgccat cgacgttgta ggcctcaagg cccagaccga agtggtcgat agacgaattc
4500
tgcatcgtca ccgacgtgct gttgcgcacg aacaagcccg cccccttcga gagcgaaggg
4560
tcaccagtgc cgccgctgaa ccgcacgccg tccaaaacga tgttggccga accctggatc
4620
gtattcagtc gattccagtc atcggcgggc ttgtaatcgg tcgcagcgac catgtttttg
4680
acggtaacgt tgctactgtt cccgatcacc agcttttgga tattgaccgg gttcgacgag
4740
tcgagcgact caattgtcac catgctggta aacgtcttgg tcattacagt gagatctgtg
4800
tagaccccgg cggcaagctt gatggtttcg ccacccttcg ccgccgcgat tgcagcattc
4860
aactccgtct gattcttgac aatgatatcc ggcatgttga cttaccccgt acgcacgaac
4920
ccgggccgat attgaccctt ccattgtcat aaataccaga acagccatga aatttgctcg
4980
aagggataca gttaagaact cccttctacg gggccgcatg ccgggcccat gcacgcccga
5040
ctttcgccgg caccgtctcg acggcgcaac acagtgcagc tactagggtg cgatgcagat
5100
gctcccaacg cccgatgtca gcatactcgt ggtcgctttc aactcgaccg agtatatcga
5160
agactgcctg cgcggcatcg ccgaaggagc gggcaagacc ccccacgaag ttctgctgat
5220
cgacaatggc gacgggcgaa ccgaagcgct ggtccggcag cggttccacc acgtccgcat
5280
cgttcccagt gagggcaata ttggtttcgg ggccggcaat aatcgcctgg cagcgcaggc
5340
tgccggcccg ctcctgctgc tcgtcaaccc cgatgccatt ccccagcccg gcgcaatcga
5400
tcagttggtc acctttgcca aacagcatcc cgaggcggcg gcatggggcg gccgttccta
5460
ctcgcccagc ggcgatctag aacccgcaaa tttcatgtcc ctgccgacgc ccgccgactt
5520
tctgacggcg attttcaacg cgcgtgcgct acgcagcggc gggctgcaag aaggcgcgac
5580
cacccccgga gcggtcgagg tgttgaatgg cggcttcatg atggtacgca ccgatgtctg
5640
gcaggcgatc ggcggttttg acgagagctt ttttctttat tcggaagaga tcgatctctt
5700
ccagcgaatc cgcacgttgg ggcacaaggt gctcgtcgac ccctcggtca aagtggtaca
5760
caatacgggg agtggtcagt cgatgtccca gaaccgcctg atgtatctca cgaccgggcg
5820
catgcactat gcgcgaaagc attttggcgc actcggcacc cttgccaccg ggtgcgcgct
5880
ttggctgatc gccgccaaat acacgttggt cggggcggca ctctggcgcc tgtcgccgcg
5940
gacgggcacg cgatacaaag agctgagcaa cgggtggcgt gccgtattta gcaatcctgg
6000
ccgatggtgg agcggctatc cgcgtcgcta aaagtccagc tccccccccc ctaaaggcgc
6060
cgttgggagg cggacgcatc gttgcaacaa cgcgcccgcc tttcagacct tcagttcccc
6120
gccggcgttg cgccgctgcc gcgaagctgc ggcggtgcgc tgtagccggc ctgatatttc
6180
acggtttccc gcgccttctt caggcggtcg ttgagctgtg cgtcagccgc cttgccgaag
6240
cgctcggtac gcagcccgct gagcgcgatc tcgcgcgcct ggtcggccgg caccggcagc
6300
accgtggtcg acgtgatgat attcgcggtc agtccctgct gggtcggcag gatgaacatc
6360
tcctgtgccg gcagcgacgc gatcttggca gcgatttccg gcggcagcgc agcggtgtcg
6420
atctgcgacg gcgcgcgacg gaactggaca ttgtccgccg agagcttggc ggttagctgg
6480
tccagcgtct tcagcggcgc gaattgcttg agctttgcgg ccgagctcgg cggagcgaag
6540
acgacctgat cgatcgcgta gatcttgcgc tgcgcgaacc gctccggatg cgcggcctga
6600
tatttctcga tctcggcatc ggtcggctgg gcgatgccgc cggcgatctt gtcgcgcagc
6660
atggcggtga ggatcagctc gtcggcccgg cgctcctgga tcaggaaggc aggcgtcttg
6720
tccagcttct gctcgcgggc gaccttggcg aggatcttgc gctcgatgat gcgctgcagc
6780
gccagctgct cggccagctt gcgatcggtc cccgggggta cctgggaggc ctgcagttcg
6840
gcattcagct cgaagacggt gatttcttcg ccatcgacgc tggcgaccac ctgccccttg
6900
tcgagcttgc cgcccttgcc gccacatccg gagacggcca gcgcggccgc agccaccgcc
6960
gtaaccaggt acaatttctt catgaagacc tccccgccgg cacggaattg cgcacggcac
7020
aaacttctac ttgaacctat tcggacgggc gggcatccgc aatagcgttg gcagtgcagc
7080
atggttctaa gcggagccag gcggcaacaa gggggacgag atggcagaag cgaacgcggt
7140
agatggaaag gcctccaagc cgctgaaaat gtgccttgca gcgtcgggcg gcggccatct
7200
ccggcaaatc ctcgatctgg aatcggtgtg gcgcgaacac gattatttct tcgttactga
7260
agataccgcg ctcggccgga gccttgccga aaaacatccc gtcgaactgg tggagcacta
7320
tgcgctcggc caggccaagc tgggccatcc cttgcgcatg ctgggcggcg catggcgcaa
7380
cctgcgccag agcctttcga tcctgcgccg gcacaagccg gatgtggtga tttccaccgg
7440
cgcgggcgca gtctatttca ccgcgctgct cgccaaactg tcgggcgcca agttcgtcca
7500
tatcgaaagc ttcgcgcgct tcgaccaccc gtctgccttc ggcaagatgg tgaagggcat
7560
cgcgacggtg acgatcgtcc agtcggcggc gctgaaagaa acctggcctg atgccgagct
7620
gttcgatccg ttccgcctgc tcgatacacc gcgcccgccc aagcaggcgc taatcttcgc
7680
gacggtcggc gccaccctgc ccttcccgcg gctggtgcag gcagtgctcg acctgaagcg
7740
cgccggcggg ctgccgggca agctgatcct gcaatatggc gaccaggacc tgcccgatcc
7800
cggcatcccc gacgtcgaga tccgccgtac catcccgttc gacgatctgc agctgctgct
7860
gcgcgatgcg gatatggtga tatgccacgg cggcaccgga tcgctggtca cggcgctgcg
7920
cgccggctgc cgggtcgtcg cctttccgcg ccgccacgat ctgggcgagc attatgacga
7980
tcaccaggaa gagatcgccc agaccttcgc cgaccggggc ctgctccagg cggtgcgcga
8040
cgagcgccag ctcggcgccg ctgtggaagc ggccaaggca accgagccgc agctggcgac
8100
caccgaccac acggccctcg cggcgcggct gcgccagctg ctggcgcagt ggagtgccaa
8160
gcgatgagca cgccccggat cagcgtcgtc atcccgcact ataacgatcc gcaatccttg
8220
cggctctgcc tggatgcgct ggagcggcag acgatcggtc gcgacgcgtt cgagatcatc
8280
gtcggcgaca acaattcgcc ctgtgggctc gcggcggtgg aggcggcggt cgccggacgt
8340
gcgcggatcg tgaccattct ggaaaagggg gcgggccccg cgcgcaacgg ggcggcagcc
8400
gcagcgcgtg gcgagatcct cgcctttacc gacagtgact gcgtggtgga gcccggctgg
8460
ctggcgggcg gcacgaccag ggtcgcgcct ggccgtttca tcggcgggca catgtatgtg
8520
cgcaagcccg aagggccgcc gaacggcgcc gaggcgctgg agatggcgct ggcgttcgac
8580
aatgaaggct atgtgcggcg cacccagttc acggtcaccg caaacctgtt cgtgatgcgc
8640
gccgatttcg aacgggtcgg cggcttccgc gttggcgtgt ccgaggatct ggaatggtgc
8700
caccgggcga tcgccagcgg cctcaccatc aactatgcac cggatgcatc ggtgggccac
8760
ccgccccggc ccgactggtc ggccctgctg gtgaagacgc ggcgcatcca gcgcgaactc
8820
tatctgttca acatcgagcg gccgaagggc aggctgcgct ggctggtccg ttccgtggcg
8880
caaccggcga tgatcccaca ggacgtggcc aagatcctgc gcacaccggg taccaagggc
8940
gcgcgcctcg ctgcggtcac cacgctggtc cggctgcggc tgtggcgcgg cggcgccggc
9000
ttgttgcagt tgctcggccg cgacatctga tcgaccggcg atcggccgac gagcgcgtcg
9060
ccggccgatc gcattgcatc agacggtggc cagcgcgtct tccagcgtgc cgctgtcgag
9120
ccgcaggcgg ccgatcatca gccacagata gaccggcagc gtatcgtcgg tgaagcggaa
9180
gcggcaatcg ccgtcctgcg tttcggattc gaggccgagt tgaccggtga gctcgcccag
9240
ctcctgctcg acctgcgccg ccgtgatgtg cgcgcccggc agcagatcca ccacggcttg
9300
gccgctgaac cagccatccg ccgagcgcga ggcctcgccc agcgccgcga cgagtggatc
9360
gtagcggccg ccgacgaact tgcgcatctc gatcaccgcg cgcggcggca tgcggccctc
9420
gatctcaagg atcgcctggt cgagcgcacg acgcagatgc ccggcgtcga ccgtgaggcg
9480
gccctggtcc agggcttcca gcgcggaatg gtggcacagc agccgcgcga aatagggcga
9540
ccccagcgcg agcaggtgga tcatgtgagt caggtccgga tcgaagcgaa cgcccgaggc
9600
ggtttcgccg agcgcgatca tctcctgcac ctccgattcc tccagccggg gcatcggcag
9660
gccgatgacg ttgcggcgga tcgacggcgc ataaccgatc agctcctgca ggttcgaggc
9720
gacgcccgcg atcaccagct ggacgcgcgc cgaacggtcc gacaggttct tgatcagctc
9780
ggcgacctgc tgacggaagg cggaatcgct gacgcgatca tattcgtcga ggatgatcag
9840
cacgcgtgtg cccgtgatgt cggcgcacag gtcggccagt tcgccgggcc cgaagctgcc
9900
cgtcggcagg cggtcggcca agttgccgcc gctctccgcc tcgccggcgt tgggcgccac
9960
gccgcgatgg aacagcagcg gcacgtcttc cagcacggcg cggaagacat cgctgaaatt
10020
cgcgttcgca ccgcaggtcg catagctgac gatatagctg gattcgcggg cgacatcggt
10080
cagcacgtgg agcagcgagg tcttgccgat gccgcgctcg ccatagagca cgacatggct
10140
gcgctggctc tcgatcgagg agattaggcg cgccagcacg ccgaggcgcc cggcgaagct
10200
cgaccgatcg gccaccggct gggtgggtgt gaagaaggtc gccagcgcga accgggcgcg
10260
cgtgatctcg cggcgctcgt cgcggcggcg atccagcggg cggtccagcg cggaggcacg
10320
gaaggttggg aaatccgggc gaccacggcc gctatgggca tcgcgatgcg gcaccactgt
10380
cgcagtcagc gggaaatagc cctcttcttc aggttcttct cgacggccga acggccacaa
10440
gaatctcagc gcggaaccta cagccactcg aacacctctt aaattcgtgc gccatcggca
10500
ccgacggcgc accctggttc gcgccccctg gcgccccctc ctaacgaacc cacgccttgc
10560
ctggcctatc ggcgcttgaa gaactcgtac ggtttgatca ccaaggcgat gtacgccagg
10620
accagagcga tcgtcaaaat tgcaaagacg tgataattct cattgcccag ataattggcg
10680
acggcgcaac cgactgcggg cggcaaatag ctgatcatcg tgtcccggac tgccgaatcg
10740
gcttgggacc gttgcaggaa tataacgatc aggccggcaa atatcgcgat ggtgacccaa
10800
tcatagggcg tctgcatgca tgtcctttct attcgacacc ggaatcgaac catttccggc
10860
gacgctattg cacgcactag cagtgcgcgc ggccgctcgc taggtagcgc cgcaccggat
10920
aaaccgacgt taagatggcg cggctcgatc gaaatggagt caaacgggct tgcccggccg
10980
accgaagcat ggcgccatgg cgcatgcacc gtattgtgac cacgcaaacc gcgagggtca
11040
ttcgatgcgg ttgcttgtac aggaggccat tgataatgaa gccgagaccc gggggaacct
11100
ttatgcaagt aaatttcaat cgacaggctc gcaagctcgg tgccggcaat gcgctcgcgc
11160
ggggggggcc cgtgcttgcg ctgcttgcga ccgcggcatg gacacaacct gcgctggcgc
11220
agcgacaggc atttgagtcc cgcccctccg gtagcgagcg acaggtcgat attcgcgcga
11280
cggggtcgct ggaatatgac gacaacgtcg tgctgaacga ccagcggatc acggacggcg
11340
cgcgtggcga tgtgatcgca tcgcccgggc tggacgtgac cctagttctg ccccgcgcca
11400
ccgggcagct ctacctcacc ggcaatgtcg gatatcgctt ttacaagcga tataccaact
11460
ttaaccgcga gcagatctcg ctcaccggcg gcgcagatca gcggttcgcc tcctgcgtcg
11520
tgcacgggga agtcggctat cagcgccacc tcaccgacct gtccagcatc ttgatccagg
11580
acaccacgcc tgcgctcaac aacaccgaag aggcccggca gtacaccgcg gatatcggct
11640
gcggcgcgac ctacggcctg cggcctgccg tttcctacac ccgcaacgaa gtgcgcaaca
11700
gccttgccga gcgccgatac gcggactcga ataccaacac ctttaccgca cagcttggcc
11760
tgacttcgcc tgccctgggg accgtggcgg tatttgggcg tatgtccgac agcagctatg
11820
tccatcgcgt ccttcccggc attaccggcc aggacgggat gaagagctac gcggccggcg
11880
tccagctcga gcgctcggtg gccaaccgac tccatttcaa cggctcggtg aattacaccg
11940
aggttgaccc aaagctcgca tccaccaaag gattcaaggg cgtaggattt aacgtttccg
12000
gcgattatgc tggtgatcag tacagcctcc aattgctggc ttcacgatcg ccccagcctt
12060
cacttcttct gttcgtgggt tacgagattg tgacagcggt ttcggcgaat gcgacgcgcc
12120
ggctgagcga tcgcattcag atatcgctgc aaggcagccg aacctggcgc gagctcgcgt
12180
cttcgcggct gctcaccaac gtgccgattt ccggcaacga caacacctcg acgttgttcg
12240
cctccgctac cttccggccg aatcgccggc tgagctttgt gctgggtgcc ggccttcagc
12300
ggcgcaccag caacacgcag ctatacagtt acagctccaa acgcatcaat ctctcgacgt
12360
cgctttcgct ctgacaaggg ccgtaatcat gcatatcaag aatcgcttcg tgaatatctc
12420
gacgttggcc atcgccgccg cgctggccac gccggcggcg gcgcagatcc ccacgcggtc
12480
cgtgcccgcg ccggcccgcc cgcggcctgc aacgccgccg gcgcaacagc agaaccaggc
12540
gccgtcgacg cccgcagcgg caaccccggc gcagaccgcc gcaaccgttg cccctgcagc
12600
aaccgcaccc gcaggttaca aaatcggcgt ggacgacgtg atcgaggccg acgtgctcgg
12660
ccagaccgac ttcaagacgc gcgcccgtgt gcaggcggac ggcacggtga ccctgcccta
12720
tctgggcgcc gtgcaggtca agggcgagac cgcgacctcg ctcgccgaaa agctggccgg
12780
gctgctgcgc gccggcggct attatgccaa gccgatcgtc agcgtcgaaa tcgtcggttt
12840
cgtcagcaac tatgtgacgg tgctgggcca ggtgaacagt tccggcctgc agccggtcga
12900
ccgcggctat cacgtttccg agatcatcgc ccgtgccggc ggcctgcgcc ccgaagcggc
12960
cgatttcgtc gttctcaccc gcgccgatgg ctccagcgcc aagctggact acaagaagct
13020
cgcccaaggt ggccccaatg acgatccgat ggtgacgccc ggggacaagg tctttgtccc
13080
ggaagtcgag catttctaca tttatggtca aattaacgcg cctggcgtat acgcgattcg
13140
atcggacatg acgctccgtc gcgcgctggc ccagggcggt gggcttgccc ccgcaggctc
13200
cgtcaagcgt gtgaaggtca cgcgggatgg caatgaactc aagttgaagc tggacgatcc
13260
gattctccca ggcgacacga tcgtcatcgg cgaacgattg ttctgatctt ggcaacgatg
13320
gcagcggacg aggcccacca gtgaatatca ttcagttctt ccgcattctg tgggtgcgcc
13380
gatggatcat cctcccggcg tttctcgttt gcgttaccac tgccaccatt gtggtccagt
13440
ttctgcccga acgctacaag gccactacgc gggtggtgct cgacacgttt aagcccgatc
13500
ccgtcaccgg acaggtgatg agctcgcagt tcatgcgcgc ctatgtcgag actcagaccc
13560
agctgatcga ggactatgcg accgccggtc gcgtggtcga cgaactgggc tgggtgaatg
13620
atccggcgaa catctccgcg ttcaacaact cgtccgcggc tgccaccggc gacatccgcc
13680
gctggctcgc caagcagatc atcgacaata ccaaggccga tgtgatggag gggagcaaca
13740
tcctcgaaat cacctattcg gacagctcgc ccgagcgcgc cgaacgcatc gccaacctga
13800
tccgcacctc gttcctcgcc cagtcgctcg ccgccaagcg ccaggccgcg accaagtcgg
13860
ccgactggta cgcccagcag gccgaagctg cccgcgattc gctcgctgcg gcggtccagg
13920
cccgcaccga tttcgtgaag aagaccggca tcgtgctgac cgaaaccggc gccgacctgg
13980
aaacccagaa gctccagcag atcgaggggc agacgacgac cgccaccgcc ccggttgcca
14040
tggcccccag cggcatgggc ccggcgcaga tgcagctcgc ccagatcgac cagcagatcc
14100
agcaggcagc gaccagccta ggtccgaacc acccaacttt ccaggccttg cagcggcagc
14160
gcgaagtgtt cgccaaggca gcggcggcgg aacgcgcgca ggcgaacggc gtatccggtc
14220
cggcacgcgg ggccatcgaa agcgcagcca acgcccagcg cgcgcgggtt ctcggcaatc
14280
gtcaggatgt cgacaagctt acgcagctgc agcgtgacgt ctcgctgaag caggatcagt
14340
acatgaaggc ggcacagcgc gtcgccgatc tgcggctgga agcaagcagc aacgatgtcg
14400
gcatgtcgac gctcagcgaa gcatcggcgc cggaaacgcc ctattacccc aaggtgccgc
14460
tcatcatcgg tggtgcagcc ggcttcggcc tcgggctcgg tctgctggtc gcgctgctcg
14520
tcgagctgct cggccgccgc gtccgcagcc ccgaggatct ggaagttgcg atcgatgcac
14580
cggtgctggg cgtgatccag agccgcgcct cgcttgccgc ccgccttcgc cgcgcccaag
14640
aaaccctcgg cgaaggtgcc gacacgcacg gagcttcagt aaactgatgg acgcgatgac
14700
cagcgaaccg ctgcccgaag gcgatcgtcc gagcgccgtg ccgaccacgc cggatacgat
14760
cggcatgctc gaataccagc tcgtcctctc cgatccgacc gggatcgagg cggaagcgat
14820
ccgcgcgcta cgcacgcgca tcatgaccca gcacctccgc gagggccggc gcgcgctcgc
14880
gatctgcgcc gcctcggcgg gatccggctg cagcttcacc gccgtcaatc tggcgacggc
14940
gctggcgcag atcggcgtta agactgcgct ggtcgatgcc aatctgcgcg atcccagcat
15000
cggcgcagcc ttcggcctcg ccgccgacaa gcccggcctg gccgattatc tcgcctcggg
15060
cgatgtcgac ctcgcctcga tcatccatgc gacccgcctc gaccagctct cgatcatccc
15120
ggccgggcat gtcgagcaca gcccgcagga actgctcgcg tccgaacagt tccatgatct
15180
ggcgacgcag ctgctgcgcg agttcgacat cacgatcttc gacaccacgg cgtccaacac
15240
ctgcgccgac gcgcagcgtg tcgcgcatat cgccggctat gcgatcatcg tggcgcgcaa
15300
ggatgcgagc tacatccgcg acgtgaacac gctcagccgc acgctgcgtg cagaccgcac
15360
caacgtcatc ggctgcgtac tgaacggcta ttgatttgga ccatatggca gcgaccgcga
15420
tgacgcggca gcaggagagg aagggcggtg gctattggct ggccgttgcc ggtcttgccg
15480
cgctaaccat cccgaccttc atcaccctgg gtcgcgaggt ttggagtgcg gaaggcggcg
15540
tgcagggtcc gatcgtgctc gccacgggcg cctggatgct ggcccgccag tgctcgacga
15600
tcgaggcgct acgccgcccc ggcagcgtgc tgctcggcgc gctgttcctg ctggcgacgc
15660
ttgccttcta caccgttgga cgggtgttcg acttcatcag tgtcgaaacc ttcggactgg
15720
tcgcgaccta tctggtcgtc gcctatctct atttcggtgc cagggtgctc cgtgccgcct
15780
ggttcccggt gctgtggctg ttcttcctgg tgccgccgcc cggctgggcc gtcgaccgca
15840
tcaccgcacc gctcaaggag ttcgtctcct atgcggcaac gggcctgctt tcctgggtgg
15900
attatccgat cctgcgccag ggcgtgacac tgttcgtcgg cccctatcag ctgctcgtcg
15960
aagatgcctg ttcgggtctg cgctcgctgt ccagcctggt cgtcgtgacg ctgctctaca
16020
tctacatcaa gaacaagccg tcctggcgct acgcggcgtt catcgcagcg ctggtgatcc
16080
cggtggcagt ggtgaccaac gtcctgcgga tcatcatcct ggtactgatc acctatcatc
16140
tgggcgacga ggcggcgcag agcttcctcc acgtctccac cggcatggtg atgttcgtgg
16200
tcgccctgct ttgcatcttc gcgatcgact gggtggtcga gcaacttctt ctcctgcgtc
16260
ggaggcatca tgttcaaccg gcgtgacctg ctgatcggcg caggctgctt cgccgccgct
16320
ggcgcctcgc tcggcctgaa gccgcaccgg cggatggacc tgctgggcgg caccaagctc
16380
gacacgctga tgcccaaggc attcggcgca tggaaggcag aggataccgg ttcgctgatc
16440
gcgccggcgc gcgaaggcag cctggaggac aagctctaca accaggtggt cacccgcgcc
16500
ttctcccgcg cggacggtgc ccaagtgatg ctgctgatcg cctatggcaa cgcccagacc
16560
gatctactgc agctgcaccg gccggaaata tgctacccgt tcttcggctt caccgtggtg
16620
gaaagccatg agcagaccat cccggtgacg ccgcaggtga cgatccccgg tcgcgcgctg
16680
accgccacca acttcaaccg caccgagcag atcctctact ggacccgcgt cggcgaatat
16740
ctgccgcaga acggcaatca gcagatgctc gcgcggctga agagccaggt ccagggctgg
16800
atcgtcgacg gtgtgctggt gcgcatctcg acggtgacgc ccgaggcgga agatggcctg
16860
agcgccaatc tcgatttcgc gcgcgagctg gtgaagacgc tcgacccgcg cgtgctgcgc
16920
ccgctgctcg ggaacgggct cacacggcag ctcggtcacc aggtctgaac cggtgcgccg
16980
cacgcggcgc ccccggcaac aaaaaaggag cggcgcgggc cgccgccgct ccctctcctt
17040
ctcatgcggc gccctgccct caccgctcgt gcagcgcgtc actccccgtc tcgagcacgg
17100
gccccaccag atagctgaac agggttcgct tgccggtgac gatgtccgcg ctcgcgagca
17160
tccccggccg cagcggcacc tgtgcgccat gggccagcac atacccgcgc gccagcgcga
17220
tccgcgcctt gtagaccggc ggctggttct ccttcatctg caccgcctcg gggctgatgc
17280
ccgccaccgt gccgggaatc atgccgtagc gggtataggg aaaggcctgc agcttcacct
17340
ttaccggcat gccgatgtgg acgaagccga tgtcgctgtt gtcgaccatc acctcggcct
17400
cgagccgggc attgtcggga accaggctga ggagcggctt ggccccttcc accacgccgc
17460
cttcggtgtg gacctgcagc tgcgagacgg taccgctcac cggcgcgcgc agttcgcgga
17520
acgagctgcg cagattcgcc ttggcgacgt cctcgccgcg ggcacgcacc tcgtcctgcg
17580
ccttgaccag atcctgcagc acctgcgccc gcgcctcctc gcgcgtcttg gccgacaggc
17640
tggagacgct cagcgactgc tggccgagtt tggcgagcgt agcgcgcgcc gccgtcaggt
17700
cctgccgctc ggcgatcagc tggcgacgca tctccacgac gcgcagcttc gagacatagc
17760
ccttggcggc catcgtctcg ttcgcggcga tctgctgttc gagcagcggc agcgactgtt
17820
cgagcttccg cacctgtgcc tgcgcctcgg ccgcggccga gacggcggca ccgcgatcgg
17880
agcggccgcc ggccagcgcc gcctcgatct ggcccagccg ggcgcgggcg aggccgcgat
17940
gcgtcgccac ttcgcccggg ctggcggcgg caggcgcgac gaagcggaag cccctgccgt
18000
ccagcgcgtc gatgatcgcc tggttgcgtg cggcgtcgag ctgggcgctg agcagcgcca
18060
ccttcgcctg tgccgcctcc gccgacgaca cggtcgggtc gagcgtgatc agcacctggc
18120
ccttggcgac cttctgcccc tcgcccacca ggatgcggcg gacgatcccc gattcgggcg
18180
actggacgat cttggtctcg ccgatcggcg cgatccgccc ctgcgtcggc gcgacgactt
18240
cgaccttgcc gatcgccagc caggcggcgg tgatcgccag cccggccagc atcaccttgg
18300
cggtaagccg cgcggtgggc gaaaccggcc gctcgatgat ctccagcgcg gcaggcagga
18360
aggcggtgtc ataagcgtcg acgcgggcag gcagcacggt atcgcgcatg cgggcgagcg
18420
ggccgccgcg gcgcatcgga acaacggcgt tcatgcggca atctccccat agccgccctg
18480
gcggcggtgc aggtcggcat agcggccgcc caggcgcaac aattcgtcgt gtcggccgct
18540
ctcgacgatg cggccctgtt cgagcgtgat gatccggtcg cagctgcgca ccgcgctcag
18600
gcgatgcgcg atcaccacga gcgtgcggcc ggccgagatg gcgcgcaggt tgttctggat
18660
cagctcctcg ctctcggcat cgagcgccga ggtcgcttcg tcgaacacca ggatgcgcgg
18720
attgccgacg agcgcgcggg cgatggcgag ccgctggcgc tggccgccgg agagattgac
18780
gccgcgctcg acgatctcgg tgtcatagcc gcgcggctgg cgcaggatga aatcatgcgc
18840
gccggccagc gtcgccgccg cgacgacatt ctcgaacggc atggcggggt tggagagcgc
18900
gatgttctcg cggatcgagc ggctgaacag cagattctcc tgcagcacga cgccgatctg
18960
gcgacgcagc caggcgggat cgagctgcgc cacgtcgacc tcgtcgacca gcacgcggcc
19020
gagattcggc aggttgagcc gctggagcag cttggccagc gtcgacttgc ccgagcccga
19080
cgaaccgacg atgccgagcg aggtgcccgc cggaatgtcg agcgtgatgt cgctcagcac
19140
cggcggctgg tcctcggcat agcggaagct gacattctcg aagcgaatcg caccgcgcag
19200
caccggcagc gtcgccgccg aggccgggcg cggttccacc ggatggttga gcacgtcgcc
19260
cagccgctcg accgagatgc gcacctgctg gaaatcctgc cacagctgcg ccatgcggat
19320
caccggcccg gacacgcgct gggcgaacat gttgaacgcc accagcgcgc ctacgctcat
19380
cgcgccgccg atcaccgcct tggcgccgaa gaacaggatc gccgcgaagc tcagcttcga
19440
gatcagctcg atcgcctggc tgccggtgtt ggcggtattg atcagccgct gcgacgcggc
19500
ggtatgggcg gcgagctggc gctcccagcg attctgccag tgcggctcga ccgcggtcgc
19560
cttgatcgtg tggatgcccg agacgctctc gacgagcagc gcgttgctgg cggagctctt
19620
ctcgaacttg tcctccaccc gcgcgcggag cggcccggcg acgctgaacg atacgatcgc
19680
ataggcgatc agcgacacga gcacgatgcc cgagagcatc ggcgagtaga acagcatcgc
19740
ggcgaggaac acgaaggtga acagcgggtc caccatcacc gtcagcgagg cgctggtaag
19800
gaattcgcgg atcgtctcga gctggcggac gcgggtgacg gtgtcgccca cgcggcgctt
19860
ctcgaaatag gcgagcggca gcgccagcag gtggtggaac agccgggcac ccagctcgac
19920
gtcgatcttc tgcgtcgtct cggtgaacag gcgggtgcgg atccagccga gcgccacttc
19980
ccacaccgaa accgccagga aggcgaaggc gagcacgctc agcgtgctca tgctgttgtg
20040
gatcagcacc ttgtcgatca cgctctggaa caacagcggc gcggcgaggc cgagcaggtt
20100
gagcgcgagg gtgatgccga gcacctcgag gaacagcgtg cgatagcgcc ggaactgcgc
20160
ggtgaaccag gagaggccga accgcagcgg ccgtcccgcc accgcgcggg tggtgagcag
20220
caccagcgcg ccggaccaga tcgcgtccag cgcgtcccgg tcgacctgtt ccggggcatg
20280
gcccgggcgc tggatgatca cgccatgttc ggtcaggccg ccgatcacga accagccttc
20340
gggcccgtcg gcgatcgcgg gcagcggctg gcgggcgagt ccgccgcgcg gcacctcgac
20400
ggccttggcg cgcacgccct gctggcgctt ggccaggagg atcaggtcgt cggcgcttgc
20460
cgcctcggca tggcccagcg cgtggcgcag ctgttcgggc gtgatggcga tgttgtgcgc
20520
gccgagcagc agcgacaacg ccaccagtcc ggattcgcgc agctccgcct cgcgctccgc
20580
cgccccatgg gccgcgagcg cgctctgcag ggtggcctgc atttcgtcgc gtgtcatttc
20640
cggaactctg cctccatggc gatactgaga gcgccatgat gaagaaggct ggtaaagact
20700
cacttaatcc tagcttttct ggtatttacc cgtagctgcc gacccgattt gggacaggcc
20760
tggcttagca ggtccttaaa ctcgaccgac tataccgcga cgccgaggag ggggaggatt
20820
ggcgccgcat cgcgcggcga aacgcgggtg cgtcgcaaca tttcgccgga gtcgatccgt
20880
cgcgaatgct gcacccgcga acgcaatgac ggccgccacg caatccggct tgatcccggg
20940
cggcggatcg cgataagccg cgccacggtc gccaaaactc gtcgaaataa ccgacaaaac
21000
cacggcatat ggctggatat tgcagcgttt gccctgcgtt tccgtcgttc aaccgccctt
21060
cgaatcaggc aggcccagcg tgaccatgat tgatcttcct cttggaacgg cacactttgg
21120
tcgacacgga gacttccggt cgggcaattg tcccgttata gtgcaatgca acaggccgaa
21180
tcggccgctg tcggcgtgca cattccgttg agggagcccg atgaggcaat gaacgctttc
21240
gaagcacagc gcgcctttga ggagcaactt cgggcgcatt cccgggttac gccatctgcc
21300
gctcccgtgt ggcgtcgctc gacgctgcgg atggtcctct ataccgagtt gctgctgctg
21360
gacagtctct cgatcctggc cggattccac gtcgcggcgg gcacgcgcga cggcaactgg
21420
ctgtcgctgg cgggcatcaa cgtcggcgtc ttcctgctgc cgatcgctct cggcaccgcg
21480
ctcgcaagcg gcacctactc gctgaactgc ctgcgctacc cggtcagcgg cgtgaagagc
21540
atcttctcgg cattcttctt ctcgatcttc gtcgtcctgc tcggcagcta cctgctgacg
21600
gccgagctgc cgctgtcccg cgtgcagctg gcggagggcg cgatcctctc gctggtcctc
21660
ctgatggtgg gccgcctgat gttccgccgc cacgtccgcg cggttaccgg cggcaggctg
21720
ctcgacgaac tggtcatcat cgacggcgtc tcgctcgacg tcgcgggcaa tgcggtcgcg
21780
ctcgacgcgc ggatcatcaa tctctcgccg aacccgcgcg atccgcaaat gctgcatcgc
21840
ctgggcacca ccgtgatcgg gttcgaccgg gtgatcgtcg cctgcaccaa ggagcatcgc
21900
gcggtctggg cgctgctgct caagggcatg aacatcaagg gcgagatcct cgtcccccag
21960
ttcaatgcgc tgggcgcgat cggcgtggac gcctttgacg ggaaggatac gctggtcgtc
22020
tcgcagggcc cgctcaacat gcccaaccgc gcgaagaagc gcgcgctcga tctcgcgatc
22080
accgtaccgg ccgtgctcgc gctggcgccg ctgatgatcc tggtggcgat cctgatcaag
22140
ctggagagcc cgggcccggt gttgttcgcg caggatcgcg tcggccgcgg caaccggctg
22200
ttcaagatca tgaagttccg ctcgatgcgc gtaacgctgt gcgacgcgaa cggcaacgtc
22260
tcggccagcc gcgacgacga tcgcatcacc aaggtcggcc gcttcatccg caagaccagc
22320
atcgacgaac tgccgcagct gctgaacgtg ctgcgcggcg acatgagcgt cgtcggcccg
22380
cggccgcatg cgctgggctc gcgcgccgcc gatcacctgt tctgggaaat cgacgagcgc
22440
tactggcacc gccacacgct caagccgggc atgaccggtc tggcccaggt gcgcggtttc
22500
cgcggggcga ccgatcgccg cgtcgatctg accaaccggc tccaggcaga catggaatat
22560
atcgacggat gggatatctg gcgcgatatc acgatcctgt tcaagacgct gcgggtgatc
22620
gtgcattcga acgcattctg atccgcgcac gacgctgggc cgcagcctcg atccgcaaat
22680
ggattgacag cggcccggct tccgttttct cgtttgattt tcgttgcggc cggtccgcgc
22740
catgggggat tactgaatga agggcatcat ccttgcgggg ggcagcggga cgcgcctgta
22800
ccccgcaacg ctatcgatct cgaagcagct gcttcccgtc tatgacaagc cgatgatctt
22860
ctatccgctg tcggtgctga tgctcaccgg catccgggac atcctgatta tctccacccc
22920
gcgcgacctg ccgatgttcc aggcgctgct gggcgacggc tcggccttcg gcatcaacct
22980
cagctatgcc gagcagccct cccccaacgg gctggccgaa gcgttcatca tcggcgcgga
23040
tttcgtcggc aacgatccca gcgcgctgat cctgggcgac aacatctatc acggcgaaaa
23100
gatgggcgag cgctgccagg cagccgcagc gcaggcagcg cagggcggtg caaacgtctt
23160
cgcctatcat gtcgacgacc ccgagcgcta cggcgtggtc gcgttcgacc cggagacggg
23220
cgtcgccacc agcgtcgagg aaaagccggc cgagcccaag tccaactggg cgatcaccgg
23280
cctgtatttc tacgacaagg acgtggtcga catcgccaag tcgatccagc cctcggcgcg
23340
cggcgaactc gagatcaccg acgtcaaccg cgtttacatg gagcgcggcg acctgcacat
23400
cacgcgcctc ggccgcggct atgcctggct cgacaccggc acgcatgaca gcctgcacga
23460
agccggctcg ttcgttcgca cgctcgagca tcggacgggc gtgaagatcg cctgcccgga
23520
ggaaatcgcc ttcgaaagcg gctggctcgg cgccgaagac ctgctcaagc gcgccgccgg
23580
cctcggcaag accggctatg ccgcctatct ccgcaaggtt gcgaccgcag catgacccag
23640
gtccatcatc acgaactgtc cggcgtcatc gagttcacgc cgcccaaata tggcgaccac
23700
cgcggcttct tctccgaagt gttcaagcag tcggtgctcg atgccgaagg cgtcgaggca
23760
cgctgggtgc aggacaatca gagcttctcg gcggccccgg gcacgatccg cggcctgcat
23820
ctccaggcgc cgcccttcgc ccaggccaag ctggtccgcg tgttgcgcgg cgcgatcttc
23880
gacgtcgcgg tcgacatccg tcgcggctcg cccacctatg gcaaatgggt cggcgtcgag
23940
ctctcggccg agaagtggaa ccagctgctg gtccccgccg gctatgcgca cggcttcatg
24000
acgctcgttc cggattgcga gatcctctac aaggtcagcg ccaaatattc gaaggattcg
24060
gagatggcga tccgttggga cgatcccgat ctcgccatcg cctggccgga catcggcgtc
24120
gagccggtcc tctccgaaaa ggacgcggtc gccacgccct tcgccgaatt caacaccccc
24180
ttcttctatc agggctgagc catgcagcag accttcctcg tcaccggcgg cgccggcttc
24240
atcggctcgg cggtggtgcg ccacctcgtc cgccagggcg cgcgcgtcat caatctcgac
24300
aagctcacct atgccggcaa cccggcctcg ctgactgcga tcgagaacgc gcccaactat
24360
cgcttcgtcc atgccgacat cgccgacacc gcgacgatcc taccgctgct gcgcgaggag
24420
caggtcgatg tggtgatgca cctcgccgcc gagagccatg tcgatcgctc gatcgacggc
24480
cctggcgagt tcatcgagac caatgtcgtc ggcaccttca agctgctcca gtcggcgctg
24540
caatattggc gcgagctgga gggcgagaaa cgcgacgcgt tccgcttcca ccacatctcc
24600
accgacgaag tgttcggcga cctgccgttc gacagcggca tcttcaccga agagacgccc
24660
tatgatccct cctcgcccta ttcggcgtcg aaggcggcga gcgaccatct ggtgcgcgcc
24720
tggggccaca cctatggcct gccggtggtg ctgtcgaact gctcgaacaa ttacgggccg
24780
ttccacttcc ccgagaagct gatcccgttg accatcctca acgcgctcga gggcaagccg
24840
ctgccggtct acggcaaggg cgagaatatc cgcgactggc tgtatgtcga cgatcacgcc
24900
aaggcgctgg cgaccatcgc caccaccggc aaggtcggcc agagctacaa tgtcggcggc
24960
cgcaacgagc ggaccaacct gcaggtggtc gagacgatct gcgacctgct cgaccagcgc
25020
attccgctgg ccgacggtcg caagcgccgc gaactgatca ccttcgtcac cgatcgcccc
25080
ggccatgacc gccgctacgc gatcgacgcg accaagctcg agaccgagct gggctggaag
25140
gctgaggaga atttcgacac cggcatcgcc gcgacgatcg actggtatct ggcgaacgag
25200
tggtggtggg gcccgatccg ctccggcaaa tatgccggcg agcggctggg gcagaccgcc
25260
tgatgcgtat cctcgtcacc gggcatgacg gccaggtcgc ccagtcgctg gccgagcagg
25320
cggtgggcca cgagctggtc ttcaccacct accccgaatt cgatctctcc aagccggaga
25380
cgatcgaggc cggtgtggcg cgggtgcacc cggacctgat cgtctccgcc gccgcctaca
25440
cggcggtcga caaggcggaa agcgaacccg agctggcgat ggcgatcaac ggcgacggtc
25500
ccggcgtgct ggcgcgcgcg ggcgcgaaga tcggcgcgcc gatcatccac ctgtcgaccg
25560
attatgtgtt cgacggcagt ctcgaccgcc cttggcgcga ggacgatccc accggcccgc
25620
tcggcgtcta tggcgcgacc aagctggccg gcgagcaggc ggtgcaggcc tcgggtgcca
25680
ccaacgccgt gatccggctg gcctgggtct acagcccgtt cggcaacaat ttcgtcaaga
25740
cgatgctccg cctcgccgag acgcgcgacg cgctgaacgt cgtggaggac cagtggggct
25800
gccccagttc ggcgctggac atcgcgaccg cgatcctgac ggtggtcggg cactggcagc
25860
aggacggcgc gacgagcggc ctctaccatt tcgccggcac cggcgagacc aactgggccg
25920
acttcgcatc gacgatcttc gccgagagcg ccaagcgcgg tggcccctcg gccaccgtca
25980
ccggcattcc cagctcgggc tatccgactc cggccacgcg cccggccaat tcgcggctgg
26040
actgcacccg cttcgcggag accttcggct accgggcgcc tgcctggcag gattcgctga
26100
acgtcgtact ggatcgcctg ctcggctgat ccgaaacggg gggcctcagc gccccccgcc
26160
atgctcccgt tcgcgcgccg gcaatgcctc tagcaccgcg cgctttccct taggactcag
26220
ctcgctccag ccggcgattt ccttgggcga ccgccagcac cccaggcaca gccggatc
26278
<210>2
<211>1053
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1053)
<223>dpsS(部分)
<400>2
atc aac ggc gcc ttg ctc gga cgg cac aaa ttc gtc ctg gtc aat gtg
48
Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val
1 5 10 15
tcc acg gtc gcc tct tcg ata ctg ttc cag ctg ttc ccg ctt gtc gtc
96
Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val
20 25 30
gcc tgg atg atc ggc ccg gac ctg cga acg ctg ctg atc gcc gcg ctc
144
Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu
35 40 45
gtt ggc cgg gcg gtt ccg atg atc ggc atg ctg ccc gcg ctg tat cga
192
Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg
50 55 60
aac ctt ttg cgc ggc aac acg ccg cgt ttt cac gcc agc gag gcg cgc
240
Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg
65 70 75 80
ttc ctg ata ggc tat ggc ggg tgg gcc tcg ctc acg acc gtg gta gcg
288
Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala
85 90 95
acc gtg ctc atg atg gcg gac cgc ttc ctg att ggc gca ctt ctt ggg
336
Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly
100 105 110
ccc gtc gcc gtg acc atc tac acg gcc ccc ctg caa ctc gca cag cgc
384
Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg
115 120 125
gta tcg ctg ctg ccc tcc gca ctg tcc gcc gcg ctg ttc ccg cgc ctg
432
Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu
130 135 140
ccc agc gcg acg ccg gcg gag cgc atg gcg ctt cag atc cgc tcg ctg
480
Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu
145 150 155 160
tcg ctg atc atg ggc ggc ctt acc ggg atg atc ggc ggc gga cta ttg
528
Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu
165 170 175
ctg gcc gcg ccg ttt ctc gat ctc tgg atc ggc aag tcg ctc ggc cat
576
Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His
180 185 190
gcg gga acg ccg gtc gcg ctc ttc ctg ttc ttc ggc gca tgg tgg aat
624
Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn
195 200 205
gcg ctg gcg atc att tcg ttc agc ggc ctg cag gcg agc gga cgg ccg
672
Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro
210 215 220
aaa gcg agc gcg atc gtc cag ggg gca gag ctg cta ccc gtg ttg atc
720
Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile
225 230 235 240
gcg ctg tat gca ggg atc cga tgg ggc ggc gtg acc ggc gcc gca gcg
768
Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala
245 250 255
gtc ttt ctg gga cgc tcc gcc ctg gat ttc gtc ctg ttg acc tgg cag
816
Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln
260 265 270
gca ggc ctg ctc cgc cag acg gtg aag caa gta tcc gta tgc ggc gcc
864
Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala
275 280 285
gtt ctc acc gtc gcg atg ctc gtg ggc gcg acc tat cgc tat tcg gtg
912
Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val
290 295 300
ccg ctc tgg tgc gta ctc agc gcc tgc tgc ctg gtc gcg ctg gca gcc
960
Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala
305 310 315 320
tgc tcc tgg tgg aca ttg gcg cgc cag gac aag gca ctg ctg att gga
1008
Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly
325 330 335
cga ttg agc cga att cta cca aag cag cgg caa ctc gac cta tag
1053
Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu *
340 345 350
<210>3
<211>350
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>与gelS同源
<400>3
Ile Asn Gly Ala Leu Leu Gly Arg His Lys Phe Val Leu Val Asn Val
1 5 10 15
Ser Thr Val Ala Ser Ser Ile Leu Phe Gln Leu Phe Pro Leu Val Val
20 25 30
Ala Trp Met Ile Gly Pro Asp Leu Arg Thr Leu Leu Ile Ala Ala Leu
35 40 45
Val Gly Arg Ala Val Pro Met Ile Gly Met Leu Pro Ala Leu Tyr Arg
50 55 60
Asn Leu Leu Arg Gly Asn Thr Pro Arg Phe His Ala Ser Glu Ala Arg
65 70 75 80
Phe Leu Ile Gly Tyr Gly Gly Trp Ala Ser Leu Thr Thr Val Val Ala
85 90 95
Thr Val Leu Met Met Ala Asp Arg Phe Leu Ile Gly Ala Leu Leu Gly
100 105 1l0
Pro Val Ala Val Thr Ile Tyr Thr Ala Pro Leu Gln Leu Ala Gln Arg
115 120 125
Val Ser Leu Leu Pro Ser Ala Leu Ser Ala Ala Leu Phe Pro Arg Leu
130 135 140
Pro Ser Ala Thr Pro Ala Glu Arg Met Ala Leu Gln Ile Arg Ser Leu
145 150 155 160
Ser Leu Ile Met Gly Gly Leu Thr Gly Met Ile Gly Gly Gly Leu Leu
165 170 175
Leu Ala Ala Pro Phe Leu Asp Leu Trp Ile Gly Lys Ser Leu Gly His
180 185 190
Ala Gly Thr Pro Val Ala Leu Phe Leu Phe Phe Gly Ala Trp Trp Asn
195 200 205
Ala Leu Ala Ile Ile Ser Phe Ser Gly Leu Gln Ala Ser Gly Arg Pro
210 215 220
Lys Ala Ser Ala Ile Val Gln Gly Ala Glu Leu Leu Pro Val Leu Ile
225 230 235 240
Ala Leu Tyr Ala Gly Ile Arg Trp Gly Gly Val Thr Gly Ala Ala Ala
245 250 255
Val Phe Leu Gly Arg Ser Ala Leu Asp Phe Val Leu Leu Thr Trp Gln
260 265 270
Ala Gly Leu Leu Arg Gln Thr Val Lys Gln Val Ser Val Cys Gly Ala
275 280 285
Val Leu Thr Val Ala Met Leu Val Gly Ala Thr Tyr Arg Tyr Ser Val
290 295 300
Pro Leu Trp Cys Val Leu Ser Ala Cys Cys Leu Val Ala Leu Ala Ala
305 310 315 320
Cys Ser Trp Trp Thr Leu Ala Arg Gln Asp Lys Ala Leu Leu Ile Gly
325 330 335
Arg Leu Ser Arg Ile Leu Pro Lys Gln Arg Gln Leu Asp Leu
340 345 350
<210>4
<211>1625
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1626)
<223>dpsG
<400>4
atg acg act acc tcg gcg ttt cgt cgc ccg gcc ttc cac gga gcg atg
48
Met Thr Thr Thr Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met
1 5 10 15
cag cgg ctt cgc agg ttg cga ctg gtt cgg ttt ctg aca aag cca gcg
96
Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala
20 25 30
atc ccg gta ctg ccc gtc tac aaa gcc gag cga tca ggc gtg acg atc
144
Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile
35 40 45
gcg gcg cgg cgt acc gtt ctg ctg gtc agc gtg atg ttt ctt gcc gca
192
Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala
50 55 60
gtc tac ggc ctg ctc gcc gca gtt ctg ccg ctc cag atg ctg gcg atc
240
Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile
65 70 75 80
ccg gcc gtg ccc ctc gtt ctg atg gcg ctc gta gtg atc tgg gcg cta
288
Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu
85 90 95
ccc gag gcg cgg cag gcg cct act cgc ctg ctg gca aaa cta tac ctc
336
Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu
100 105 110
gcc tat atg gtg gcg gcg ctc gtg tgg ccg aac tat ctt gcg ctc agc
384
Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser
115 120 125
gtt ggt ggt ttg cct tgg atc tcg atc agg cgg atg atc ggt tcg atc
432
Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile
130 135 140
gca ttg ctc acg ctc atg atc tcg ctt tcg gtc tcg aaa aag ttt cga
480
Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg
145 150 155 160
tcc gag atg gcg gcg att atg cgg gcg gcg ccg atc cca tcg cga cta
528
Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu
165 170 175
ttg ctt gcc ttc att atg gtc cag atc gtc gcc tcc att gcg acc cca
576
Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro
180 185 190
gcg gcc tcg caa aca att ccg cgg ttg att ggc atc gtt ctg acg gtg
624
Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val
195 200 205
acg ccg atg gca ttc atc tcg cta tgg ctg atc ggg acc gac acg cgc
672
Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg
210 215 220
acc ccg gag tgg tgg gtt acg cgc ttg ttt tgg tgc gtg ggc gtg ctg
720
Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu
225 230 235 240
atg gcg att ggc gtg ctg gag ttc cgg gtg aag cac gtc ctc tgg gcc
768
Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala
245 250 255
tac tcg att ccg agc ttc ctg cgc gtt gat gag cag ttt ctt acc gta
816
Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val
260 265 270
gtg ctt acg ccc ggt ttc cgg ggt act tac cgc gtt ttg acg act ttc
864
Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe
275 280 285
agc agt ccg ctt gtc tgg gga gag ttg acg gct ttg acg att cct ttc
912
Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe
290 295 300
gtg ctg cac cgt ata gcg aat tcc cgt ggc gta ggg aga ttg gca ttt
960
Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe
305 310 315 320
tgg att ttc ttc gat ttt ctt gta gtt gcg tcc gga ttt ctt agc ggt
1008
Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly
325 330 335
tct cga ctg gca atg gtg ggg gga ttg gtg gcg cat acc gtg tat ttg
1056
Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu
340 345 350
ctt att tgg gca att cgg cgg tgg cgg acc aca aag ggc ggg ctt gtc
1104
Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val
355 360 365
ggc att tcg ctg acg ttg acg tat ccc gcc ttg atg gtg gcg ctg tcg
1152
Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser
370 375 380
ctg gcg gtc atg ttc gtg cct gcc gtc cac aac cgt gtt ctc ggt gga
1200
Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly
385 390 395 400
ggc gct tct caa ctt agc aat caa ggc agg cag gag cag ttt cgc ttg
1248
Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu
405 410 415
ggc gtg cct gcc atc gct cgc cgc cct ttc ttc ggc tat ggc ccg gga
1296
Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly
420 425 430
gag ggt gct ggc gct gtc ggg tgg cgt aat cag caa ggc ttc cta tcg
1344
Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser
435 440 445
ate gac agt ggt ttc ctg tcc gta gcg gct gat tac ggc ttg ctg ggt
1392
Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly
450 455 460
ttt gtt tcg cta tac ggc acg atg att acg tta atg att ttg ctc gcg
1440
Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala
465 470 475 480
ttt cgt ggt ctt aag atg agc gga gat ggc tac ccg ctt gag ctc gcc
1488
Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala
485 490 495
gta gca act ttc ctg gct gtt ctt tta aat acg cgg tca gtc ctg tcg
1536
Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser
500 505 510
caa ggt gac aat gac ccg ttc atc ttt atg acg ctg ggg cta ggc ata
1584
Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile
515 520 525
gcg ctt cta tat cgc tct cgg cct gtg tct ttg tcg gtg tag
1626
Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val *
530 535 540
<210>5
<211>541
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的聚合酶
<400>5
Met Thr Thr Thr Ser Ala Phe Arg Arg Pro Ala Phe His Gly Ala Met
1 5 10 15
Gln Arg Leu Arg Arg Leu Arg Leu Val Arg Phe Leu Thr Lys Pro Ala
20 25 30
Ile Pro Val Leu Pro Val Tyr Lys Ala Glu Arg Ser Gly Val Thr Ile
35 40 45
Ala Ala Arg Arg Thr Val Leu Leu Val Ser Val Met Phe Leu Ala Ala
50 55 60
Val Tyr Gly Leu Leu Ala Ala Val Leu Pro Leu Gln Met Leu Ala Ile
65 70 75 80
Pro Ala Val Pro Leu Val Leu Met Ala Leu Val Val Ile Trp Ala Leu
85 90 95
Pro Glu Ala Arg Gln Ala Pro Thr Arg Leu Leu Ala Lys Leu Tyr Leu
100 105 110
Ala Tyr Met Val Ala Ala Leu Val Trp Pro Asn Tyr Leu Ala Leu Ser
115 120 125
Val Gly Gly Leu Pro Trp Ile Ser Ile Arg Arg Met Ile Gly Ser Ile
130 135 140
Ala Leu Leu Thr Leu Met Ile Ser Leu Ser Val Ser Lys Lys Phe Arg
145 150 155 160
Ser Glu Met Ala Ala Ile Met Arg Ala Ala Pro Ile Pro Ser Arg Leu
165 170 175
Leu Leu Ala Phe Ile Met Val Gln Ile Val Ala Ser Ile Ala Thr Pro
180 185 190
Ala Ala Ser Gln Thr Ile Pro Arg Leu Ile Gly Ile Val Leu Thr Val
195 200 205
Thr Pro Met Ala Phe Ile Ser Leu Trp Leu Ile Gly Thr Asp Thr Arg
210 215 220
Thr Pro Glu Trp Trp Val Thr Arg Leu Phe Trp Cys Val Gly Val Leu
225 230 235 240
Met Ala Ile Gly Val Leu Glu Phe Arg Val Lys His Val Leu Trp Ala
245 250 255
Tyr Ser Ile Pro Ser Phe Leu Arg Val Asp Glu Gln Phe Leu Thr Val
260 265 270
Val Leu Thr Pro Gly Phe Arg Gly Thr Tyr Arg Val Leu Thr Thr Phe
275 280 285
Ser Ser Pro Leu Val Trp Gly Glu Leu Thr Ala Leu Thr Ile Pro Phe
290 295 300
Val Leu His Arg Ile Ala Asn Ser Arg Gly Val Gly Arg Leu Ala Phe
305 310 315 320
Trp Ile Phe Phe Asp Phe Leu Val Val Ala Ser Gly Phe Leu Ser Gly
325 330 335
Ser Arg Leu Ala Met Val Gly Gly Leu Val Ala His Thr Val Tyr Leu
340 345 350
Leu Ile Trp Ala Ile Arg Arg Trp Arg Thr Thr Lys Gly Gly Leu Val
355 360 365
Gly Ile Ser Leu Thr Leu Thr Tyr Pro Ala Leu Met Val Ala Leu Ser
370 375 380
Leu Ala Val Met Phe Val Pro Ala Val His Asn Arg Val Leu Gly Gly
385 390 395 400
Gly Ala Ser Gln Leu Ser Asn Gln Gly Arg Gln Glu Gln Phe Arg Leu
405 410 415
Gly Val Pro Ala Ile Ala Arg Arg Pro Phe Phe Gly Tyr Gly Pro Gly
420 425 430
Glu Gly Ala Gly Ala Val Gly Trp Arg Asn Gln Gln Gly Phe Leu Ser
435 440 445
Ile Asp Ser Gly Phe Leu Ser Val Ala Ala Asp Tyr Gly Leu Leu Gly
450 455 460
Phe Val Ser Leu Tyr Gly Thr Met Ile Thr Leu Met Ile Leu Leu Ala
465 470 475 480
Phe Arg Gly Leu Lys Met Ser Gly Asp Gly Tyr Pro Leu Glu Leu Ala
485 490 495
Val Ala Thr Phe Leu Ala Val Leu Leu Asn Thr Arg Ser Val Leu Ser
500 505 510
Gln Gly Asp Asn Asp Pro Phe Ile Phe Met Thr Leu Gly Leu Gly Ile
515 520 525
Ala Leu Leu Tyr Arg Ser Arg Pro Val Ser Leu Ser Val
530 535 540
<210>6
<211>1998
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1998)
<223>dpsR
<400>6
atg ccg gat atc att gtc aag aat cag acg gag ttg aat gct gca atc
48
Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile
1 5 10 15
gcg gcg gcg aag ggt ggc gaa acc atc aag ctt gcc gcc ggg gtc tac
96
Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr
20 25 30
aca gat ctc act gta atg acc aag acg ttt acc agc atg gtg aca att
144
Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile
35 40 45
gag tcg ctc gac tcg tcg aac ccg gtc aat atc caa aag ctg gtg atc
192
Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile
50 55 60
ggg aac agt agc aac gtt acc gtc aaa aac atg gtc gct gcg acc gat
240
Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp
65 70 75 80
tac aag ccc gcc gat gac tgg aat cga ctg aat acg atc cag ggt tcg
288
Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser
85 90 95
gcc aac atc gtt ttg gac ggc gtg cgg ttc agc ggc ggc act ggt gac
336
Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp
100 105 110
cct tcg ctc tcg aag ggg gcg ggc ttg ttc gtg cgc aac agc acg tcg
384
Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser
115 120 125
gtg acg atg cag aat tcg tct atc gac cac ttc ggt ctg ggc ctt gag
432
Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu Glu
130 135 140
gcc tac aac gtc gat ggc atg gtg gtc cag aac agc agc ttc cac gac
480
Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp
145 150 155 160
aac cgg cgc gat cat acg aac ttc act gag atg aac aat ctt gtc atc
528
Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile
165 170 175
gac gga aat tcg ttc acg aac ctg ttt ccc gtg ggc acc gaa cat ccc
576
Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro
180 185 190
gac gcc att cag ttc ttc acg gcg ggc aag gtc aag ggc aat acc aac
624
Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn
195 200 205
atc acc atc tcc aat aac gtc atc atg cag ggc gcg ggc tct ggc gcg
672
Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala
210 215 220
caa ggg att ttc atg aat gac gag gcc ggt aat ctt ccc tat gtc aat
720
Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn
225 230 235 240
gta aac atc aaa aac aat ctt atc tat ctg aat ggt tat tac cac ggt
768
Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly
245 250 255
atc aac gtt gtt aac ggc gtt aat gtc aat atc gaa tcc aat agc gtg
816
Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val
260 265 270
ata tcg caa gtg gat ggc aca tca ttt tgg att cgc ctc gac aaa acc
864
Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr
275 280 285
aat ggc gcg acg atc aag aac aat gtt gcg gac ctg atc acc gtc aca
912
Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr
290 295 300
agc tcc tcg agc aat atc gtg cag aca ggc aat cgt acg ctg acg agt
960
Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser
305 310 315 320
gac tcg gca acg atc cgc aag atc tat ggc ctc aac gat ggg gct acg
1008
Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr
325 330 335
gcg cgg ctc agc gat ttg atc gtt ccc ggc gtc ggg tac cag ccg ccc
1056
Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro
340 345 350
gtg tcg agc gct gct gcc gct cag gtg act acc gaa ctg tcg act gcg
1104
Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala
355 360 365
aag gcg gca aat ccg tcg ctg ctg ctc gat ctg tcg ttc agc aac agc
1152
Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser
370 375 380
ggc gtc gtg gac ctt tcg cac tgg aat acc ggc cag acg aca aag gcg
1200
Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala
385 390 395 400
gtg gac gtg tcg gcg gtc gtg ggc agc gcc ttc cac gtc tcg acg ggc
1248
Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly
405 410 415
acg ggg gtg gaa cta aac cgg agc tat tcg cgg cag att tac gca ttg
1296
Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu
420 425 430
tcg gcg ttc acg ctc agc ttc gac ctc aag cgg gac tcg gct acg gcc
1344
Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala
435 440 445
acg gcc ggg caa att ctt ggc atc ttc cag agc tgg tcg gtt tcg ctg
1392
Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu
450 455 460
cag gcc aat ggg gaa ctg agc ttc acc atg cgc aac gcc gcg ggc gtc
1440
Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val
465 470 475 480
agc cag aca atg gtg acg agc ggc gcc aag ctg ctt gat gct gcc aca
1488
Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr
485 490 495
cac aag atc gcc ctg acc tac gac agc acg cgg aaa acc gcg att ctg
1536
His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu
500 505 510
tac gta gac ggc atg caa cgc ggc aca gcg acg atg acc ggc acg acc
1584
Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr
515 520 525
cgg ccc gcc gaa tcc tgg ggg ctg tat gtc ggc agc ccg ttc tcg acc
1632
Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr
530 535 540
gca ttc agc gga acg gtc ggc gac atc gag atc cgc gat ggc gcg atc
1680
Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile
545 550 555 560
agc gcc gcc cag gtg cag gcg ctg gtg acc gcg tcg agc gcc agc gcg
1728
Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala
565 570 575
gcg gcg acg gtc aag gac agc ctc gtc acc ggc gcg gcc gcg cag gcc
1776
Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala
580 585 590
gct gcg ctg ctg gcg ggt gcc ggc gcc gct agc acg gca acg ccg ctt
1824
Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu
595 600 605
gcg acg gtg gcc acg gtg ggc agt acg ctg tct ata ggt act gcc gcg
1872
Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala
610 615 620
tcc tcg cag atc gcg ctc gtc agc aag atc ggt gtc gac atg atg acc
1920
Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr
625 630 635 640
gcg ggg gcg atg ggc gca atc cgc agc gcg gcg aca ctg agc gct acg
1968
Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr
645 650 655
gcg gat cag tac aac ctg tac cgc gcc tga
1998
Ala Asp Gln Tyr Asn Leu Tyr Arg Ala *
660 665
<210>7
<211>665
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的裂合酶
<400>7
Met Pro Asp Ile Ile Val Lys Asn Gln Thr Glu Leu Asn Ala Ala Ile
1 5 10 15
Ala Ala Ala Lys Gly Gly Glu Thr Ile Lys Leu Ala Ala Gly Val Tyr
20 25 30
Thr Asp Leu Thr Val Met Thr Lys Thr Phe Thr Ser Met Val Thr Ile
35 40 45
Glu Ser Leu Asp Ser Ser Asn Pro Val Asn Ile Gln Lys Leu Val Ile
50 55 60
Gly Asn Ser Ser Asn Val Thr Val Lys Asn Met Val Ala Ala Thr Asp
65 70 75 80
Tyr Lys Pro Ala Asp Asp Trp Asn Arg Leu Asn Thr Ile Gln Gly Ser
85 90 95
Ala Asn Ile Val Leu Asp Gly Val Arg Phe Ser Gly Gly Thr Gly Asp
100 105 110
Pro Ser Leu Ser Lys Gly Ala Gly Leu Phe Val Arg Asn Ser Thr Ser
115 120 125
Val Thr Met Gln Asn Ser Ser Ile Asp His Phe Gly Leu Gly Leu GLu
130 135 140
Ala Tyr Asn Val Asp Gly Met Val Val Gln Asn Ser Ser Phe His Asp
145 150 155 160
Asn Arg Arg Asp His Thr Asn Phe Thr Glu Met Asn Asn Leu Val Ile
165 170 175
Asp Gly Asn Ser Phe Thr Asn Leu Phe Pro Val Gly Thr Glu His Pro
180 185 190
Asp Ala Ile Gln Phe Phe Thr Ala Gly Lys Val Lys Gly Asn Thr Asn
195 200 205
Ile Thr Ile Ser Asn Asn Val Ile Met Gln Gly Ala Gly Ser Gly Ala
210 215 220
Gln Gly Ile Phe Met Asn Asp Glu Ala Gly Asn Leu Pro Tyr Val Asn
225 230 235 240
Val Asn Ile Lys Asn Asn Leu Ile Tyr Leu Asn Gly Tyr Tyr His Gly
245 250 255
Ile Asn Val Val Asn Gly Val Asn Val Asn Ile Glu Ser Asn Ser Val
260 265 270
Ile Ser Gln Val Asp Gly Thr Ser Phe Trp Ile Arg Leu Asp Lys Thr
275 280 285
Asn Gly Ala Thr Ile Lys Asn Asn Val Ala Asp Leu Ile Thr Val Thr
290 295 300
Ser Ser Ser Ser Asn Ile Val Gln Thr Gly Asn Arg Thr Leu Thr Ser
305 310 315 320
Asp Ser Ala Thr Ile Arg Lys Ile Tyr Gly Leu Asn Asp Gly Ala Thr
325 330 335
Ala Arg Leu Ser Asp Leu Ile Val Pro Gly Val Gly Tyr Gln Pro Pro
340 345 350
Val Ser Ser Ala Ala Ala Ala Gln Val Thr Thr Glu Leu Ser Thr Ala
355 360 365
Lys Ala Ala Asn Pro Ser Leu Leu Leu Asp Leu Ser Phe Ser Asn Ser
370 375 380
Gly Val Val Asp Leu Ser His Trp Asn Thr Gly Gln Thr Thr Lys Ala
385 390 395 400
Val Asp Val Ser Ala Val Val Gly Ser Ala Phe His Val Ser Thr Gly
405 410 415
Thr Gly Val Glu Leu Asn Arg Ser Tyr Ser Arg Gln Ile Tyr Ala Leu
420 425 430
Ser Ala Phe Thr Leu Ser Phe Asp Leu Lys Arg Asp Ser Ala Thr Ala
435 440 445
Thr Ala Gly Gln Ile Leu Gly Ile Phe Gln Ser Trp Ser Val Ser Leu
450 455 460
Gln Ala Asn Gly Glu Leu Ser Phe Thr Met Arg Asn Ala Ala Gly Val
465 470 475 480
Ser Gln Thr Met Val Thr Ser Gly Ala Lys Leu Leu Asp Ala Ala Thr
485 490 495
His Lys Ile Ala Leu Thr Tyr Asp Ser Thr Arg Lys Thr Ala Ile Leu
500 505 5l0
Tyr Val Asp Gly Met Gln Arg Gly Thr Ala Thr Met Thr Gly Thr Thr
515 520 525
Arg Pro Ala Glu Ser Trp Gly Leu Tyr Val Gly Ser Pro Phe Ser Thr
530 535 540
Ala Phe Ser Gly Thr Val Gly Asp Ile Glu Ile Arg Asp Gly Ala Ile
545 550 555 560
Ser Ala Ala Gln Val Gln Ala Leu Val Thr Ala Ser Ser Ala Ser Ala
565 570 575
Ala Ala Thr Val Lys Asp Ser Leu Val Thr Gly Ala Ala Ala Gln Ala
580 585 590
Ala Ala Leu Leu Ala Gly Ala Gly Ala Ala Ser Thr Ala Thr Pro Leu
595 600 605
Ala Thr Val Ala Thr Val Gly Ser Thr Leu Ser Ile Gly Thr Ala Ala
610 615 620
Ser Ser Gln Ile Ala Leu Val Ser Lys Ile Gly Val Asp Met Met Thr
625 630 635 640
Ala Gly Ala Met Gly Ala Ile Arg Ser Ala Ala Thr Leu Ser Ala Thr
645 650 655
Ala Asp Gln Tyr Asn Leu Tyr Arg Ala
660 665
<210>8
<21l>939
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(939)
<223>dpsQ
<400>8
atg cag atg ctc cca acg ccc gat gtc agc ata ctc gtg gtc gct ttc
48
Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe
1 5 10 15
aac tcg acc gag tat atc gaa gac tgc ctg cgc ggc atc gcc gaa gga
96
Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly
20 25 30
gcg ggc aag acc ccc cac gaa gtt ctg ctg atc gac aat ggc gac ggg
144
Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly
35 40 45
cga acc gaa gcg ctg gtc cgg cag cgg ttc cac cac gtc cgc atc gtt
192
Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val
50 55 60
ccc agt gag ggc aat att ggt ttc ggg gcc ggc aat aat cgc ctg gca
240
Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala
65 70 75 80
gcg cag gct gcc ggc ccg ctc ctg ctg ctc gtc aac ccc gat gcc att
288
Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile
85 90 95
ccc cag ccc ggc gca atc gat cag ttg gtc acc ttt gcc aaa cag cat
336
Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His
100 105 110
ccc gag gcg gcg gca tgg ggc ggc cgt tcc tac tcg ccc agc ggc gat
384
Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp
115 120 125
cta gaa ccc gca aat ttc atg tcc ctg ccg acg ccc gcc gac ttt ctg
432
Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu
130 135 140
acg gcg att ttc aac gcg cgt gcg cta cgc agc ggc ggg ctg caa gaa
480
Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu
145 150 155 160
ggc gcg acc acc ccc gga gcg gtc gag gtg ttg aat ggc ggc ttc atg
528
Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met
165 170 175
atg gta cgc acc gat gtc tgg cag gcg atc ggc ggt ttt gac gag agc
576
Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser
180 185 190
ttt ttt ctt tat tcg gaa gag atc gat ctc ttc cag cga atc cgc acg
624
Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Ile Arg Thr
195 200 205
ttg ggg cac aag gtg ctc gtc gac ccc tcg gtc aaa gtg gta cac aat
672
Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn
210 215 220
acg ggg agt ggt cag tcg atg tcc cag aac cgc ctg atg tat ctc acg
720
Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr
225 230 235 240
acc ggg cgc atg cac tat gcg cga aag cat ttt ggc gca ctc ggc acc
768
Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr
245 250 255
ctt gcc acc ggg tgc gcg ctt tgg ctg atc gcc gcc aaa tac acg ttg
816
Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu
260 265 270
gtc ggg gcg gca ctc tgg cgc ctg tcg ccg cgg acg ggc acg cga tac
864
Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr
275 280 285
aaa gag ctg agc aac ggg tgg cgt gcc gta ttt agc aat cct ggc cga
912
Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg
290 295 300
tgg tgg agc ggc tat ccg cgt cgc taa
939
Trp Trp Ser Gly Tyr Pro Arg Arg *
305 310
<210>9
<211>312
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的鼠李糖基转移酶IV
<400>9
Met Gln Met Leu Pro Thr Pro Asp Val Ser Ile Leu Val Val Ala Phe
1 5 10 15
Asn Ser Thr Glu Tyr Ile Glu Asp Cys Leu Arg Gly Ile Ala Glu Gly
20 25 30
Ala Gly Lys Thr Pro His Glu Val Leu Leu Ile Asp Asn Gly Asp Gly
35 40 45
Arg Thr Glu Ala Leu Val Arg Gln Arg Phe His His Val Arg Ile Val
50 55 60
Pro Ser Glu Gly Asn Ile Gly Phe Gly Ala Gly Asn Asn Arg Leu Ala
65 70 75 80
Ala Gln Ala Ala Gly Pro Leu Leu Leu Leu Val Asn Pro Asp Ala Ile
85 90 95
Pro Gln Pro Gly Ala Ile Asp Gln Leu Val Thr Phe Ala Lys Gln His
100 105 110
Pro Glu Ala Ala Ala Trp Gly Gly Arg Ser Tyr Ser Pro Ser Gly Asp
115 120 125
Leu Glu Pro Ala Asn Phe Met Ser Leu Pro Thr Pro Ala Asp Phe Leu
130 135 140
Thr Ala Ile Phe Asn Ala Arg Ala Leu Arg Ser Gly Gly Leu Gln Glu
145 150 155 160
Gly Ala Thr Thr Pro Gly Ala Val Glu Val Leu Asn Gly Gly Phe Met
165 170 175
Met Val Arg Thr Asp Val Trp Gln Ala Ile Gly Gly Phe Asp Glu Ser
180 185 190
Phe Phe Leu Tyr Ser Glu Glu Ile Asp Leu Phe Gln Arg Iie Arg Thr
195 200 205
Leu Gly His Lys Val Leu Val Asp Pro Ser Val Lys Val Val His Asn
210 215 220
Thr Gly Ser Gly Gln Ser Met Ser Gln Asn Arg Leu Met Tyr Leu Thr
225 230 235 240
Thr Gly Arg Met His Tyr Ala Arg Lys His Phe Gly Ala Leu Gly Thr
245 250 255
Leu Ala Thr Gly Cys Ala Leu Trp Leu Ile Ala Ala Lys Tyr Thr Leu
260 265 270
Val Gly Ala Ala Leu Trp Arg Leu Ser Pro Arg Thr Gly Thr Arg Tyr
275 280 285
Lys Glu Leu Ser Asn Gly Trp Arg Ala Val Phe Ser Asn Pro Gly Arg
290 295 300
Trp Trp Ser Gly Tyr Pro Arg Arg
305 310
<210>10
<211>972
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(972)
<223>dpsI
<400>10
atg ctg cac tgc caa cgc tat tgc gga tgc ccg ccc gtc cga ata ggt
48
Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly
1 5 10 15
tca agt aga agt ttg tgc cgt gcg caa ttc cgt gcc ggc ggg gag gtc
96
Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val
20 25 30
ttc atg aag aaa ttg tac ctg gtt acg gcg gtg gct gcg gcc gcg ctg
144
Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu
35 40 45
gcc gtc tcc gga tgt ggc ggc aag ggc ggc aag ctc gac aag ggg cag
192
Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln
50 55 60
gtg gtc gcc agc gtc gat ggc gaa gaa atc acc gtc ttc gag ctg aat
240
Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn
65 70 75 80
gcc gaa ctg cag gcc tcc cag gta ccc ccg ggg acc gat cgc aag ctg
288
Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu
85 90 95
gcc gag cag ctg gcg ctg cag cgc atc atc gag cgc aag atc ctc gcc
336
Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala
100 105 110
aag gtc gcc cgc gag cag aag ctg gac aag acg cct gcc ttc ctg atc
384
Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile
115 120 125
cag gag cgc cgg gcc gac gag ctg atc ctc acc gcc atg ctg cgc gac
432
Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp
130 135 140
aag atc gcc ggc ggc atc gcc cag ccg acc gat gcc gag atc gag aaa
480
Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys
145 150 155 160
tat cag gcc gcg cat ccg gag cgg ttc gcg cag cgc aag atc tac gcg
528
Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala
165 170 175
atc gat cag gtc gtc ttc gct ccg ccg agc tcg gcc gca aag ctc aag
576
Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys
180 185 190
caa ttc gcg ccg ctg aag acg ctg gac cag cta acc gcc aag ctc tcg
624
Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser
195 200 205
gcg gac aat gtc cag ttc cgt cgc gcg ccg tcg cag atc gac acc gct
672
Ala Asp Asn Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala
210 215 220
gcg ctg ccg ccg gaa atc gct gcc aag atc gcg tcg ctg ccg gca cag
720
Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln
225 230 235 240
gag atg ttc atc ctg ccg acc cag cag gga ctg acc gcg aat atc atc
768
Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile
245 250 255
acg tcg acc acg gtg ctg ccg gtg ccg gcc gac cag gcg cgc gag atc
816
Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile
260 265 270
gcg ctc agc ggg ctg cgt acc gag cgc ttc ggc aag gcg gct gac gca
864
Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala
275 280 285
cag ctc aac gac cgc ctg aag aag gcg cgg gaa acc gtg aaa tat cag
912
Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln
290 295 300
gcc ggc tac agc gca ccg ccg cag ctt cgc ggc agc ggc gca acg ccg
960
Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro
305 310 315 320
gcg ggg aac tga
972
Ala Gly Asn *
<210>11
<211>323
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>未知
<400>11
Met Leu His Cys Gln Arg Tyr Cys Gly Cys Pro Pro Val Arg Ile Gly
1 5 10 15
Ser Ser Arg Ser Leu Cys Arg Ala Gln Phe Arg Ala Gly Gly Glu Val
20 25 30
Phe Met Lys Lys Leu Tyr Leu Val Thr Ala Val Ala Ala Ala Ala Leu
35 40 45
Ala Val Ser Gly Cys Gly Gly Lys Gly Gly Lys Leu Asp Lys Gly Gln
50 55 60
Val Val Ala Ser Val Asp Gly Glu Glu Ile Thr Val Phe Glu Leu Asn
65 70 75 80
Ala Glu Leu Gln Ala Ser Gln Val Pro Pro Gly Thr Asp Arg Lys Leu
85 90 95
Ala Glu Gln Leu Ala Leu Gln Arg Ile Ile Glu Arg Lys Ile Leu Ala
100 105 110
Lys Val Ala Arg Glu Gln Lys Leu Asp Lys Thr Pro Ala Phe Leu Ile
115 120 125
Gln Glu Arg Arg Ala Asp Glu Leu Ile Leu Thr Ala Met Leu Arg Asp
130 135 140
Lys Ile Ala Gly Gly Ile Ala Gln Pro Thr Asp Ala Glu Ile Glu Lys
145 150 155 160
Tyr Gln Ala Ala His Pro Glu Arg Phe Ala Gln Arg Lys Ile Tyr Ala
165 170 175
Ile Asp Gln Val Val Phe Ala Pro Pro Ser Ser Ala Ala Lys Leu Lys
180 185 190
Gln Phe Ala Pro Leu Lys Thr Leu Asp Gln Leu Thr Ala Lys Leu Ser
195 200 205
Ala Asp Ash Val Gln Phe Arg Arg Ala Pro Ser Gln Ile Asp Thr Ala
210 215 220
Ala Leu Pro Pro Glu Ile Ala Ala Lys Ile Ala Ser Leu Pro Ala Gln
225 230 235 240
Glu Met Phe Ile Leu Pro Thr Gln Gln Gly Leu Thr Ala Asn Ile Ile
245 250 255
Thr Ser Thr Thr Val Leu Pro Val Pro Ala Asp Gln Ala Arg Glu Ile
260 265 270
Ala Leu Ser Gly Leu Arg Thr Glu Arg Phe Gly Lys Ala Ala Asp Ala
275 280 285
Gln Leu Asn Asp Arg Leu Lys Lys Ala Arg Glu Thr Val Lys Tyr Gln
290 295 300
Ala Gly Tyr Ser Ala Pro Pro Gln Leu Arg Gly Ser Gly Ala Thr Pro
305 310 315 320
Ala Gly Asn
<210>12
<211>1047
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1047)
<223>dpsK
<400>12
atg gca gaa gcg aac gcg gta gat gga aag gcc tcc aag ccg ctg aaa
48
Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys
1 5 10 15
atg tgc ctt gca gcg tcg ggc ggc ggc cat ctc cgg caa atc ctc gat
96
Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp
20 25 30
ctg gaa tcg gtg tgg cgc gaa cac gat tat ttc ttc gtt act gaa gat
144
Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp
35 40 45
acc gcg ctc ggc cgg agc ctt gcc gaa aaa cat ccc gtc gaa ctg gtg
192
Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val
50 55 60
gag cac tat gcg ctc ggc cag gcc aag ctg ggc cat ccc ttg cgc atg
240
Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met
65 70 75 80
etg ggc ggc gca tgg cgc aac ctg cgc cag agc ctt tcg atc ctg cgc
288
Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg
85 90 95
cgg cac aag ccg gat gtg gtg att tcc acc ggc gcg ggc gca gtc tat
336
Arg His Lys Pro Asp Val Val lle Ser Thr Gly Ala Gly Ala Val Tyr
100 105 110
ttc acc gcg ctg ctc gcc aaa ctg tcg ggc gcc aag ttc gtc cat atc
384
Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile
115 120 125
gaa agc ttc gcg cgc ttc gac cac ccg tct gcc ttc ggc aag atg gtg
432
Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val
130 135 140
aag ggc atc gcg acg gtg acg atc gtc cag tcg gcg gcg ctg aaa gaa
480
Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu
145 150 155 160
acc tgg cct gat gcc gag ctg ttc gat ccg ttc cgc ctg ctc gat aca
528
Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr
165 170 175
ccg cgc ccg ccc aag cag gcg cta atc ttc gcg acg gtc ggc gcc acc
576
Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr
180 185 190
ctg ccc ttc ccg cgg ctg gtg cag gca gtg ctc gac ctg aag cgc gcc
624
Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala
195 200 205
ggc ggg ctg ccg ggc aag ctg atc ctg caa tat ggc gac cag gac ctg
672
Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu
210 215 220
ccc gat ccc ggc atc ccc gac gtc gag atc cgc cgt acc atc ccg ttc
720
Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe
225 230 235 240
gac gat ctg cag ctg ctg ctg cgc gat gcg gat atg gtg ata tgc cac
768
Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His
245 250 255
ggc ggc acc gga tcg ctg gtc acg gcg ctg cgc gcc ggc tgc cgg gtc
816
Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val
260 265 270
gtc gcc ttt ccg cgc cgc cac gat ctg ggc gag cat tat gac gat cac
864
Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His
275 280 285
cag gaa gag atc gcc cag acc ttc gcc gac cgg ggc ctg ctc cag gcg
912
Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala
290 295 300
gtg cgc gac gag cgc cag ctc ggc gcc gct gtg gaa gcg gcc aag gca
960
Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala
305 310 315 320
acc gag ccg cag ctg gcg acc acc gac cac acg gcc ctc gcg gcg cgg
1008
Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg
325 330 335
ctg cgc cag ctg ctg gcg cag tgg agt gcc aag cga tga
1047
Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg *
340 345
<210>13
<211>348
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>β-1，4-葡糖醛酸基转移酶II
<400>13
Met Ala Glu Ala Asn Ala Val Asp Gly Lys Ala Ser Lys Pro Leu Lys
1 5 10 15
Met Cys Leu Ala Ala Ser Gly Gly Gly His Leu Arg Gln Ile Leu Asp
20 25 30
Leu Glu Ser Val Trp Arg Glu His Asp Tyr Phe Phe Val Thr Glu Asp
35 40 45
Thr Ala Leu Gly Arg Ser Leu Ala Glu Lys His Pro Val Glu Leu Val
50 55 60
Glu His Tyr Ala Leu Gly Gln Ala Lys Leu Gly His Pro Leu Arg Met
65 70 75 80
Leu Gly Gly Ala Trp Arg Asn Leu Arg Gln Ser Leu Ser Ile Leu Arg
85 90 95
Arg His Lys Pro Asp Val Val Ile Ser Thr Gly Ala Gly Ala Val Tyr
100 105 110
Phe Thr Ala Leu Leu Ala Lys Leu Ser Gly Ala Lys Phe Val His Ile
115 120 125
Glu Ser Phe Ala Arg Phe Asp His Pro Ser Ala Phe Gly Lys Met Val
130 135 140
Lys Gly Ile Ala Thr Val Thr Ile Val Gln Ser Ala Ala Leu Lys Glu
145 150 155 160
Thr Trp Pro Asp Ala Glu Leu Phe Asp Pro Phe Arg Leu Leu Asp Thr
165 170 175
Pro Arg Pro Pro Lys Gln Ala Leu Ile Phe Ala Thr Val Gly Ala Thr
180 185 190
Leu Pro Phe Pro Arg Leu Val Gln Ala Val Leu Asp Leu Lys Arg Ala
195 200 205
Gly Gly Leu Pro Gly Lys Leu Ile Leu Gln Tyr Gly Asp Gln Asp Leu
210 215 220
Pro Asp Pro Gly Ile Pro Asp Val Glu Ile Arg Arg Thr Ile Pro Phe
225 230 235 240
Asp Asp Leu Gln Leu Leu Leu Arg Asp Ala Asp Met Val Ile Cys His
245 250 255
Gly Gly Thr Gly Ser Leu Val Thr Ala Leu Arg Ala Gly Cys Arg Val
260 265 270
Val Ala Phe Pro Arg Arg His Asp Leu Gly Glu His Tyr Asp Asp His
275 280 285
Gln Glu Glu Ile Ala Gln Thr Phe Ala Asp Arg Gly Leu Leu Gln Ala
290 295 300
Val Arg Asp Glu Arg Gln Leu Gly Ala Ala Val Glu Ala Ala Lys Ala
305 310 315 320
Thr Glu Pro Gln Leu Ala Thr Thr Asp His Thr Ala Leu Ala Ala Arg
325 330 335
Leu Arg Gln Leu Leu Ala Gln Trp Ser Ala Lys Arg
340 345
<210>14
<211>867
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(867)
<223>dpsL
<400>14
atg agc acg ccc cgg atc agc gtc gtc atc ccg cac tat aac gat ccg
48
Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro
1 5 10 15
caa tcc ttg cgg ctc tgc ctg gat gcg ctg gag cgg cag acg atc ggt
96
Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly
20 25 30
cgc gac gcg ttc gag atc atc gtc ggc gac aac aat tcg ccc tgt ggg
144
Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly
35 40 45
ctc gcg gcg gtg gag gcg gcg gtc gcc gga cgt gcg cgg atc gtg acc
192
Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr
50 55 60
att ctg gaa aag ggg gcg ggc ccc gcg cgc aac ggg gcg gca gcc gca
240
Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala
65 70 75 80
gcg cgt ggc gag atc ctc gcc ttt acc gac agt gac tgc gtg gtg gag
288
Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu
85 90 95
ccc ggc tgg ctg gcg ggc ggc acg acc agg gtc gcg cct ggc cgt ttc
336
Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe
100 105 110
atc ggc ggg cac atg tat gtg cgc aag ccc gaa ggg ccg ccg aac ggc
384
Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly
115 120 125
gcc gag gcg ctg gag atg gcg ctg gcg ttc gac aat gaa ggc tat gtg
432
Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val
130 135 140
cgg cgc acc cag ttc acg gtc acc gca aac ctg ttc gtg atg cgc gcc
480
Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala
145 150 155 160
gat ttc gaa cgg gtc ggc ggc ttc cgc gtt ggc gtg tcc gag gat ctg
528
Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu
165 170 175
gaa tgg tgc cac cgg gcg atc gcc agc ggc ctc acc atc aac tat gca
576
Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala
180 185 190
ccg gat gca tcg gtg ggc cac ccg ccc cgg ccc gac tgg tcg gcc ctg
624
Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu
195 200 205
ctg gtg aag acg cgg cgc atc cag cgc gaa ctc tat ctg ttc aac atc
672
Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile
210 215 220
gag cgg ccg aag ggc agg ctg cgc tgg ctg gtc cgt tcc gtg gcg caa
720
Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln
225 230 235 240
ccg gcg atg atc cca cag gac gtg gcc aag atc ctg cgc aca ccg ggt
768
Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly
245 250 255
acc aag ggc gcg cgc ctc gct gcg gtc acc acg ctg gtc cgg ctg cgg
816
Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg
260 265 270
ctg tgg cgc ggc ggc gcc ggc ttg ttg cag ttg ctc ggc cgc gac atc
864
Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile
275 280 285
tga
867
*
<210>15
<211>288
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>葡糖基转移酶III
<400>15
Met Ser Thr Pro Arg Ile Ser Val Val Ile Pro His Tyr Asn Asp Pro
1 5 10 15
Gln Ser Leu Arg Leu Cys Leu Asp Ala Leu Glu Arg Gln Thr Ile Gly
20 25 30
Arg Asp Ala Phe Glu Ile Ile Val Gly Asp Asn Asn Ser Pro Cys Gly
35 40 45
Leu Ala Ala Val Glu Ala Ala Val Ala Gly Arg Ala Arg Ile Val Thr
50 55 60
Ile Leu Glu Lys Gly Ala Gly Pro Ala Arg Asn Gly Ala Ala Ala Ala
65 70 75 80
Ala Arg Gly Glu Ile Leu Ala Phe Thr Asp Ser Asp Cys Val Val Glu
85 90 95
Pro Gly Trp Leu Ala Gly Gly Thr Thr Arg Val Ala Pro Gly Arg Phe
100 105 110
Ile Gly Gly His Met Tyr Val Arg Lys Pro Glu Gly Pro Pro Asn Gly
115 120 125
Ala Glu Ala Leu Glu Met Ala Leu Ala Phe Asp Asn Glu Gly Tyr Val
130 135 140
Arg Arg Thr Gln Phe Thr Val Thr Ala Asn Leu Phe Val Met Arg Ala
145 150 155 160
Asp Phe Glu Arg Val Gly Gly Phe Arg Val Gly Val Ser Glu Asp Leu
165 170 175
Glu Trp Cys His Arg Ala Ile Ala Ser Gly Leu Thr Ile Asn Tyr Ala
180 185 190
Pro Asp Ala Ser Val Gly His Pro Pro Arg Pro Asp Trp Ser Ala Leu
195 200 205
Leu Val Lys Thr Arg Arg Ile Gln Arg Glu Leu Tyr Leu Phe Asn Ile
210 215 220
Glu Arg Pro Lys Gly Arg Leu Arg Trp Leu Val Arg Ser Val Ala Gln
225 230 235 240
Pro Ala Met Ile Pro Gln Asp Val Ala Lys Ile Leu Arg Thr Pro Gly
245 250 255
Thr Lys Gly Ala Arg Leu Ala Ala Val Thr Thr Leu Val Arg Leu Arg
260 265 270
Leu Trp Arg Gly Gly Ala Gly Leu Leu Gln Leu Leu Gly Arg Asp Ile
275 280 285
<210>16
<211>1389
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1389)
<223>dpsJ
<400>16
gtg gct gta ggt tcc gcg ctg aga ttc ttg tgg ccg ttc ggc cgt cga
48
Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg
1 5 10 15
gaa gaa cct gaa gaa gag ggc tat ttc ccg ctg act gcg aca gtg gtg
96
Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val
20 25 30
ccg cat cgc gat gcc cat agc ggc cgt ggt cgc ccg gat ttc cca acc
144
Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr
35 40 45
ttc cgt gcc tcc gcg ctg gac cgc ccg ctg gat cgc cgc cgc gac gag
192
Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu
50 55 60
cgc cgc gag atc acg cgc gcc cgg ttc gcg ctg gcg acc ttc ttc aca
240
Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr
65 70 75 80
ccc acc cag ccg gtg gcc gat cgg tcg agc ttc gcc ggg cgc ctc ggc
288
Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly
85 90 95
gtg ctg gcg cgc cta atc tcc tcg atc gag agc cag cgc agc cat gtc
336
Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val
100 105 110
gtg ctc tat ggc gag cgc ggc atc ggc aag acc tcg ctg ctc cac gtg
384
Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val
115 120 125
ctg acc gat gtc gcc cgc gaa tcc agc tat atc gtc agc tat gcg acc
432
Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr
130 135 140
tgc ggt gcg aac gcg aat ttc agc gat gtc ttc cgc gcc gtg ctg gaa
480
Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu
145 150 155 160
gac gtg ccg ctg ctg ttc cat cgc ggc gtg gcg ccc aac gcc ggc gag
528
Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu
165 170 175
gcg gag agc ggc ggc aac ttg gcc gac cgc ctg ccg acg ggc agc ttc
576
Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe
180 185 190
ggg ccc ggc gaa ctg gcc gac ctg tgc gcc gac atc acg ggc aca cgc
624
Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg
195 200 205
gtg ctg atc atc ctc gac gaa tat gat cgc gtc agc gat tcc gcc ttc
672
Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe
210 215 220
cgt cag cag gtc gcc gag ctg atc aag aac ctg tcg gac cgt tcg gcg
720
Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala
225 230 235 240
cgc gtc cag ctg gtg atc gcg ggc gtc gcc tcg aac ctg cag gag ctg
768
Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu
245 250 255
atc ggt tat gcg ccg tcg atc cgc cgc aac gtc atc ggc ctg ccg atg
816
Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met
260 265 270
ccc cgg ctg gag gaa tcg gag gtg cag gag atg atc gcg ctc ggc gaa
864
Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu
275 280 285
acc gcc tcg ggc gtt cgc ttc gat ccg gac ctg act cac atg atc cac
912
Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His
290 295 300
ctg ctc gcg ctg ggg tcg ccc tat ttc gcg cgg ctg ctg tgc cac cat
960
Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His
305 310 315 320
tcc gcg ctg gaa gcc ctg gac cag ggc cgc ctc acg gtc gac gcc ggg
1008
Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly
325 330 335
cat ctg cgt cgt gcg ctc gac cag gcg atc ctt gag atc gag ggc cgc
1056
His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg
340 345 350
atg ccg ccg cgc gcg gtg atc gag atg cgc aag ttc gtc ggc ggc cgc
1104
Met Pro Pro Arg Ala Val Ile Glu Met Arg Lys Phe Val Gly Gly Arg
355 360 365
tac gat cca ctc gtc gcg gcg ctg ggc gag gcc tcg cgc tcg gcg gat
1152
Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp
370 375 380
ggc tgg ttc agc ggc caa gcc gtg gtg gat ctg ctg ccg ggc gcg cac
1200
Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His
385 390 395 400
atc acg gcg gcg cag gtc gag cag gag ctg ggc gag ctc acc ggt caa
1248
Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln
405 410 415
ctc ggc ctc gaa tcc gaa acg cag gac ggc gat tgc cgc ttc cgc ttc
1296
Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe
420 425 430
acc gac gat acg ctg ccg gtc tat ctg tgg ctg atg atc ggc cgc ctg
1344
Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met lle Gly Arg Leu
435 440 445
cgg ctc gac agc ggc acg ctg gaa gac gcg ctg gcc acc gtc tga
1389
Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val *
450 455 460
<210>17
<211>462
<212>PRT
<213>鞘氨醇单孢菌(Sphinggomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>未知
<400>17
Val Ala Val Gly Ser Ala Leu Arg Phe Leu Trp Pro Phe Gly Arg Arg
1 5 10 15
Glu Glu Pro Glu Glu Glu Gly Tyr Phe Pro Leu Thr Ala Thr Val Val
20 25 30
Pro His Arg Asp Ala His Ser Gly Arg Gly Arg Pro Asp Phe Pro Thr
35 40 45
Phe Arg Ala Ser Ala Leu Asp Arg Pro Leu Asp Arg Arg Arg Asp Glu
50 55 60
Arg Arg Glu Ile Thr Arg Ala Arg Phe Ala Leu Ala Thr Phe Phe Thr
65 70 75 80
Pro Thr Gln Pro Val Ala Asp Arg Ser Ser Phe Ala Gly Arg Leu Gly
85 90 95
Val Leu Ala Arg Leu Ile Ser Ser Ile Glu Ser Gln Arg Ser His Val
100 105 110
Val Leu Tyr Gly Glu Arg Gly Ile Gly Lys Thr Ser Leu Leu His Val
115 120 125
Leu Thr Asp Val Ala Arg Glu Ser Ser Tyr Ile Val Ser Tyr Ala Thr
130 135 140
Cys Gly Ala Asn Ala Asn Phe Ser Asp Val Phe Arg Ala Val Leu Glu
145 150 155 160
Asp Val Pro Leu Leu Phe His Arg Gly Val Ala Pro Asn Ala Gly Glu
165 170 175
Ala Glu Ser Gly Gly Asn Leu Ala Asp Arg Leu Pro Thr Gly Ser Phe
180 185 190
Gly Pro Gly Glu Leu Ala Asp Leu Cys Ala Asp Ile Thr Gly Thr Arg
195 200 205
Val Leu Ile Ile Leu Asp Glu Tyr Asp Arg Val Ser Asp Ser Ala Phe
210 215 220
Arg Gln Gln Val Ala Glu Leu Ile Lys Asn Leu Ser Asp Arg Ser Ala
225 230 235 240
Arg Val Gln Leu Val Ile Ala Gly Val Ala Ser Asn Leu Gln Glu Leu
245 250 255
Ile Gly Tyr Ala Pro Ser Ile Arg Arg Asn Val Ile Gly Leu Pro Met
260 265 270
Pro Arg Leu Glu Glu Ser Glu Val Gln Glu Met Ile Ala Leu Gly Glu
275 280 285
Thr Ala Ser Gly Val Arg Phe Asp Pro Asp Leu Thr His Met Ile His
290 295 300
Leu Leu Ala Leu Gly Ser Pro Tyr Phe Ala Arg Leu Leu Cys His His
305 310 315 320
Ser Ala Leu Glu Ala Leu Asp Gln Gly Arg Leu Thr Val Asp Ala Gly
325 330 335
His Leu Arg Arg Ala Leu Asp Gln Ala Ile Leu Glu Ile Glu Gly Arg
340 345 350
Met Pro Pro Arg Ala Val lle Glu Met Arg Lys Phe Val Gly Gly Arg
355 360 365
Tyr Asp Pro Leu Val Ala Ala Leu Gly Glu Ala Ser Arg Ser Ala Asp
370 375 380
Gly Trp Phe Ser Gly Gln Ala Val Val Asp Leu Leu Pro Gly Ala His
385 390 395 400
Ile Thr Ala Ala Gln Val Glu Gln Glu Leu Gly Glu Leu Thr Gly Gln
405 410 415
Leu Gly Leu Glu Ser Glu Thr Gln Asp Gly Asp Cys Arg Phe Arg Phe
420 425 430
Thr Asp Asp Thr Leu Pro Val Tyr Leu Trp Leu Met Ile Gly Arg Leu
435 440 445
Arg Leu Asp Ser Gly Thr Leu Glu Asp Ala Leu Ala Thr Val
450 455 460
<210>18
<211>1299
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1299)
<223>dpsF
<400>18
atg aag ccg aga ccc ggg gga acc ttt atg caa gta aat ttc aat cga
48
Met Lys Pro Arg Pro Gly Gly Thr Phe Met Gln Val Asn Phe Asn Arg
1 5 10 15
cag gct cgc aag ctc ggt gcc ggc aat gcg ctc gcg cgg ggg ggg ccc
96
Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro
20 25 30
gtg ctt gcg ctg ctt gcg acc gcg gca tgg aca caa cct gcg ctg gcg
144
Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala
35 40 45
cag cga cag gca ttt gag tcc cgc ccc tcc ggt agc gag cga cag gtc
192
Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val
50 55 60
gat att cgc gcg acg ggg tcg ctg gaa tat gac gac aac gtc gtg ctg
240
Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu
65 70 75 80
aac gac cag cgg atc acg gac ggc gcg cgt ggc gat gtg atc gca tcg
288
Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser
85 90 95
ccc ggg ctg gac gtg acc cta gtt ctg ccc cgc gcc acc ggg cag ctc
336
Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu
100 105 110
tac ctc acc ggc aat gtc gga tat cgc ttt tac aag cga tat acc aac
384
Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn
115 120 125
ttt aac cgc gag cag atc tcg ctc acc ggc ggc gca gat cag cgg ttc
432
Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe
130 135 140
gcc tcc tgc gtc gtg cac ggg gaa gtc ggc tat cag cgc cac ctc acc
480
Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr
145 150 155 160
gac ctg tcc agc atc ttg atc cag gac acc acg cct gcg ctc aac aac
528
Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn
165 170 175
acc gaa gag gcc cgg cag tac acc gcg gat atc ggc tgc ggc gcg acc
576
Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr
180 185 190
tac ggc ctg cgg cct gcc gtt tcc tac acc cgc aac gaa gtg cgc aac
624
Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn
195 200 205
agc ctt gcc gag cgc cga tac gcg gac tcg aat acc aac acc ttt acc
672
Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr
210 215 220
gca cag ctt ggc ctg act tcg cct gcc ctg ggg acc gtg gcg gta ttt
720
Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe
225 230 235 240
ggg cgt atg tcc gac agc agc tat gtc cat cgc gtc ctt ccc ggc att
768
Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile
245 250 255
acc ggc cag gac ggg atg aag agc tac gcg gcc ggc gtc cag ctc gag
816
Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu
260 265 270
cgc tcg gtg gcc aac cga ctc cat ttc aac ggc tcg gtg aat tac acc
864
Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr
275 280 285
gag gtt gac cca aag ctc gca tcc acc aaa gga ttc aag ggc gta gga
912
Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly
290 295 300
ttt aac gtt tcc ggc gat tat gct ggt gat cag tac agc ctc caa ttg
960
Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu
305 310 315 320
ctg gct tca cga tcg ccc cag cct tca ctt ctt ctg ttc gtg ggt tac
1008
Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr
325 330 335
gag att gtg aca gcg gtt tcg gcg aat gcg acg cgc cgg ctg agc gat
1056
Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp
340 345 350
cgc att cag ata tcg ctg caa ggc agc cga acc tgg cgc gag ctc gcg
1104
Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala
355 360 365
tct tcg cgg ctg ctc acc aac gtg ccg att tcc ggc aac gac aac acc
1152
Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr
370 375 380
tcg acg ttg ttc gcc tcc get acc ttc cgg ccg aat cgc cgg ctg agc
1200
Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser
385 390 395 400
ttt gtg ctg ggt gcc ggc ctt cag cgg cgc acc agc aac acg cag cta
1248
Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu
405 410 415
tac agt tac agc tcc aaa cgc atc aat ctc tcg acg tcg ctt tcg ctc
1296
Tyr Ser Tyr Ser Ser Lys Arg Ile Asn Leu Ser Thr Ser Leu Ser Leu
420 425 430
tga
1299
*
<210>19
<211>432
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>未知
<400>19
Met Lys Pro Arg Pro Gly Gly Thr Phe Met Gln Val Asn Phe Asn Arg
1 5 10 15
Gln Ala Arg Lys Leu Gly Ala Gly Asn Ala Leu Ala Arg Gly Gly Pro
20 25 30
Val Leu Ala Leu Leu Ala Thr Ala Ala Trp Thr Gln Pro Ala Leu Ala
35 40 45
Gln Arg Gln Ala Phe Glu Ser Arg Pro Ser Gly Ser Glu Arg Gln Val
50 55 60
Asp Ile Arg Ala Thr Gly Ser Leu Glu Tyr Asp Asp Asn Val Val Leu
65 70 75 80
Asn Asp Gln Arg Ile Thr Asp Gly Ala Arg Gly Asp Val Ile Ala Ser
85 90 95
Pro Gly Leu Asp Val Thr Leu Val Leu Pro Arg Ala Thr Gly Gln Leu
100 105 110
Tyr Leu Thr Gly Asn Val Gly Tyr Arg Phe Tyr Lys Arg Tyr Thr Asn
115 120 125
Phe Asn Arg Glu Gln Ile Ser Leu Thr Gly Gly Ala Asp Gln Arg Phe
130 135 140
Ala Ser Cys Val Val His Gly Glu Val Gly Tyr Gln Arg His Leu Thr
145 150 155 160
Asp Leu Ser Ser Ile Leu Ile Gln Asp Thr Thr Pro Ala Leu Asn Asn
165 170 175
Thr Glu Glu Ala Arg Gln Tyr Thr Ala Asp Ile Gly Cys Gly Ala Thr
180 185 190
Tyr Gly Leu Arg Pro Ala Val Ser Tyr Thr Arg Asn Glu Val Arg Asn
195 200 205
Ser Leu Ala Glu Arg Arg Tyr Ala Asp Ser Asn Thr Asn Thr Phe Thr
210 215 220
Ala Gln Leu Gly Leu Thr Ser Pro Ala Leu Gly Thr Val Ala Val Phe
225 230 235 240
Gly Arg Met Ser Asp Ser Ser Tyr Val His Arg Val Leu Pro Gly Ile
245 250 255
Thr Gly Gln Asp Gly Met Lys Ser Tyr Ala Ala Gly Val Gln Leu Glu
260 265 270
Arg Ser Val Ala Asn Arg Leu His Phe Asn Gly Ser Val Asn Tyr Thr
275 280 285
Glu Val Asp Pro Lys Leu Ala Ser Thr Lys Gly Phe Lys Gly Val Gly
290 295 300
Phe Asn Val Ser Gly Asp Tyr Ala Gly Asp Gln Tyr Ser Leu Gln Leu
305 310 315 320
Leu Ala Ser Arg Ser Pro Gln Pro Ser Leu Leu Leu Phe Val Gly Tyr
325 330 335
Glu Ile Val Thr Ala Val Ser Ala Asn Ala Thr Arg Arg Leu Ser Asp
340 345 350
Arg Ile Gln Ile Ser Leu Gln Gly Ser Arg Thr Trp Arg Glu Leu Ala
355 360 365
Ser Ser Arg Leu Leu Thr Asn Val Pro Ile Ser Gly Asn Asp Asn Thr
370 375 380
Ser Thr Leu Phe Ala Ser Ala Thr Phe Arg Pro Asn Arg Arg Leu Ser
385 390 395 400
Phe Val Leu Gly Ala Gly Leu Gln Arg Arg Thr Ser Asn Thr Gln Leu
405 410 415
Tyr Ser Tyr Ser Set Lys Arg Ile Asn Leu Set Thr Ser Leu Ser Leu
420 425 430
<210>20
<211>918
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(918)
<223>dpsD
<400>20
atg cat atc aag aat cgc ttc gtg aat atc tcg acg ttg gcc atc gcc
48
Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala
1 5 10 15
gcc gcg ctg gcc acg ccg gcg gcg gcg cag atc ccc acg cgg tcc gtg
96
Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val
20 25 30
ccc gcg ccg gcc cgc ccg cgg cct gca acg ccg ccg gcg caa cag cag
144
Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln
35 40 45
aac cag gcg ccg tcg acg ccc gca gcg gca acc ccg gcg cag acc gcc
192
Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala
50 55 60
gca acc gtt gcc cct gca gca acc gca ccc gca ggt tac aaa atc ggc
240
Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly
65 70 75 80
gtg gac gac gtg atc gag gcc gac gtg ctc ggc cag acc gac ttc aag
288
Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys
85 90 95
acg cgc gcc cgt gtg cag gcg gac ggc acg gtg acc ctg ccc tat ctg
336
Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu
100 105 110
ggc gcc gtg cag gtc aag ggc gag acc gcg acc tcg ctc gcc gaa aag
384
Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys
115 120 125
ctg gcc ggg ctg ctg cgc gcc ggc ggc tat tat gcc aag ccg atc gtc
432
Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val
130 135 140
agc gtc gaa atc gtc ggt ttc gtc agc aac tat gtg acg gtg ctg ggc
480
Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly
145 150 155 160
cag gtg aac agt tcc ggc ctg cag ccg gtc gac cgc ggc tat cac gtt
528
Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val
165 170 175
tcc gag atc atc gcc cgt gcc ggc ggc ctg cgc ccc gaa gcg gcc gat
576
Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp
180 185 190
ttc gtc gtt ctc acc cgc gcc gat ggc tcc agc gcc aag ctg gac tac
624
Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr
195 200 205
aag aag ctc gcc caa ggt ggc ccc aat gac gat ccg atg gtg acg ccc
672
Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro
210 215 220
ggg gac aag gtc ttt gtc ccg gaa gtc gag cat ttc tac att tat ggt
720
Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly
225 230 235 240
caa att aac gcg cct ggc gta tac gcg att cga tcg gac atg acg ctc
768
Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu
245 250 255
cgt cgc gcg ctg gcc cag ggc ggt ggg ctt gcc ccc gca ggc tcc gtc
816
Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val
260 265 270
aag cgt gtg aag gtc acg cgg gat ggc aat gaa ctc aag ttg aag ctg
864
Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu
275 280 285
gac gat ccg att ctc cca ggc gac acg atc gtc atc ggc gaa cga ttg
912
Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu
290 295 300
ttc tga
918
Phe *
305
<210>21
<211>305
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的多糖输出蛋白
<400>21
Met His Ile Lys Asn Arg Phe Val Asn Ile Ser Thr Leu Ala Ile Ala
1 5 10 15
Ala Ala Leu Ala Thr Pro Ala Ala Ala Gln Ile Pro Thr Arg Ser Val
20 25 30
Pro Ala Pro Ala Arg Pro Arg Pro Ala Thr Pro Pro Ala Gln Gln Gln
35 40 45
Asn Gln Ala Pro Ser Thr Pro Ala Ala Ala Thr Pro Ala Gln Thr Ala
50 55 60
Ala Thr Val Ala Pro Ala Ala Thr Ala Pro Ala Gly Tyr Lys Ile Gly
65 70 75 80
Val Asp Asp Val Ile Glu Ala Asp Val Leu Gly Gln Thr Asp Phe Lys
85 90 95
Thr Arg Ala Arg Val Gln Ala Asp Gly Thr Val Thr Leu Pro Tyr Leu
100 105 110
Gly Ala Val Gln Val Lys Gly Glu Thr Ala Thr Ser Leu Ala Glu Lys
115 120 125
Leu Ala Gly Leu Leu Arg Ala Gly Gly Tyr Tyr Ala Lys Pro Ile Val
130 135 140
Ser Val Glu Ile Val Gly Phe Val Ser Asn Tyr Val Thr Val Leu Gly
145 150 155 160
Gln Val Asn Ser Ser Gly Leu Gln Pro Val Asp Arg Gly Tyr His Val
165 170 175
Ser Glu Ile Ile Ala Arg Ala Gly Gly Leu Arg Pro Glu Ala Ala Asp
180 185 190
Phe Val Val Leu Thr Arg Ala Asp Gly Ser Ser Ala Lys Leu Asp Tyr
195 200 205
Lys Lys Leu Ala Gln Gly Gly Pro Asn Asp Asp Pro Met Val Thr Pro
210 215 220
Gly Asp Lys Val Phe Val Pro Glu Val Glu His Phe Tyr Ile Tyr Gly
225 230 235 240
Gln Ile Asn Ala Pro Gly Val Tyr Ala Ile Arg Ser Asp Met Thr Leu
245 250 255
Arg Arg Ala Leu Ala Gln Gly Gly Gly Leu Ala Pro Ala Gly Ser Val
260 265 270
Lys Arg Val Lys Val Thr Arg Asp Gly Asn Glu Leu Lys Leu Lys Leu
275 280 285
Asp Asp Pro Ile Leu Pro Gly Asp Thr Ile Val Ile Gly Glu Arg Leu
290 295 300
Phe
305
<210>22
<211>1347
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1347)
<223>dpsC
<400>22
gtg aat atc att cag ttc ttc cgc att ctg tgg gtg cgc cga tgg atc
48
Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile
1 5 10 15
atc ctc ccg gcg ttt ctc gtt tgc gtt acc act gcc acc att gtg gtc
96
Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val
20 25 30
cag ttt ctg ccc gaa cgc tac aag gcc act acg cgg gtg gtg ctc gac
144
Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp
35 40 45
acg ttt aag ccc gat ccc gtc acc gga cag gtg atg agc tcg cag ttc
192
Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe
50 55 60
atg cgc gcc tat gtc gag act cag acc cag ctg atc gag gac tat gcg
240
Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala
65 70 75 80
acc gcc ggt cgc gtg gtc gac gaa ctg ggc tgg gtg aat gat ccg gcg
288
Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Ash Asp Pro Ala
85 90 95
aac atc tcc gcg ttc aac aac tcg tcc gcg gct gcc acc ggc gac atc
336
Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile
100 105 110
cgc cgc tgg ctc gcc aag cag atc atc gac aat acc aag gcc gat gtg
384
Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val
115 120 125
atg gag ggg agc aac atc ctc gaa atc acc tat tcg gac agc tcg ccc
432
Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro
130 135 140
gag cgc gcc gaa cgc atc gcc aac ctg atc cgc acc tcg ttc ctc gcc
480
Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala
145 150 155 160
cag tcg ctc gcc gcc aag cgc cag gcc gcg acc aag tcg gcc gac tgg
528
Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp
165 170 175
tac gcc cag cag gcc gaa gct gcc cgc gat tcg ctc gct gcg gcg gtc
576
Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val
180 185 190
cag gcc cgc acc gat ttc gtg aag aag acc ggc atc gtg ctg acc gaa
624
Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu
195 200 205
acc ggc gcc gac ctg gaa acc cag aag ctc cag cag atc gag ggg cag
672
Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln
210 215 220
acg acg acc gcc acc gcc ccg gtt gcc atg gcc ccc agc ggc atg ggc
720
Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly
225 230 235 240
ccg gcg cag atg cag ctc gcc cag atc gac cag cag atc cag cag gca
768
Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala
245 250 255
gcg acc agc cta ggt ccg aac cac cca act ttc cag gcc ttg cag cgg
816
Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg
260 265 270
cag cgc gaa gtg ttc gcc aag gca gcg gcg gcg gaa cgc gcg cag gcg
864
Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala
275 280 285
aac ggc gta tcc ggt ccg gca cgc ggg gcc atc gaa agc gca gcc aac
912
Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn
290 295 300
gcc cag cgc gcg cgg gtt ctc ggc aat cgt cag gat gtc gac aag ctt
960
Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu
305 310 315 320
acg cag ctg cag cgt gac gtc tcg ctg aag cag gat cag tac atg aag
1008
Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys
325 330 335
gcg gca cag cgc gtc gcc gat ctg cgg ctg gaa gca agc agc aac gat
1056
Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp
340 345 350
gtc ggc atg tcg acg ctc agc gaa gca tcg gcg ccg gaa acg ccc tat
1104
Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr
355 360 365
tac ccc aag gtg ccg ctc atc atc ggt ggt gca gcc ggc ttc ggc ctc
1152
Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu
370 375 380
ggg ctc ggt ctg ctg gtc gcg ctg ctc gtc gag ctg ctc ggc cgc cgc
1200
Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg
385 390 395 400
gtc cgc agc ccc gag gat ctg gaa gtt gcg atc gat gca ccg gtg ctg
1248
Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu
405 410 415
ggc gtg atc cag agc cgc gcc tcg ctt gcc gcc cgc ctt cgc cgc gcc
1296
Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala
420 425 430
caa gaa acc ctc ggc gaa ggt gcc gac acg cac gga gct tca gta aac
l344
Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn
435 440 445
tga
1347
*
<210>23
<211>448
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的多糖输出蛋白
<400>23
Val Asn Ile Ile Gln Phe Phe Arg Ile Leu Trp Val Arg Arg Trp Ile
1 5 10 15
Ile Leu Pro Ala Phe Leu Val Cys Val Thr Thr Ala Thr Ile Val Val
20 25 30
Gln Phe Leu Pro Glu Arg Tyr Lys Ala Thr Thr Arg Val Val Leu Asp
35 40 45
Thr Phe Lys Pro Asp Pro Val Thr Gly Gln Val Met Ser Ser Gln Phe
50 55 60
Met Arg Ala Tyr Val Glu Thr Gln Thr Gln Leu Ile Glu Asp Tyr Ala
65 70 75 80
Thr Ala Gly Arg Val Val Asp Glu Leu Gly Trp Val Asn Asp Pro Ala
85 90 95
Asn Ile Ser Ala Phe Asn Asn Ser Ser Ala Ala Ala Thr Gly Asp Ile
100 105 110
Arg Arg Trp Leu Ala Lys Gln Ile Ile Asp Asn Thr Lys Ala Asp Val
115 120 125
Met Glu Gly Ser Asn Ile Leu Glu Ile Thr Tyr Ser Asp Ser Ser Pro
130 135 140
Glu Arg Ala Glu Arg Ile Ala Asn Leu Ile Arg Thr Ser Phe Leu Ala
145 150 155 160
Gln Ser Leu Ala Ala Lys Arg Gln Ala Ala Thr Lys Ser Ala Asp Trp
165 170 175
Tyr Ala Gln Gln Ala Glu Ala Ala Arg Asp Ser Leu Ala Ala Ala Val
180 185 190
Gln Ala Arg Thr Asp Phe Val Lys Lys Thr Gly Ile Val Leu Thr Glu
195 200 205
Thr Gly Ala Asp Leu Glu Thr Gln Lys Leu Gln Gln Ile Glu Gly Gln
210 215 220
Thr Thr Thr Ala Thr Ala Pro Val Ala Met Ala Pro Ser Gly Met Gly
225 230 235 240
Pro Ala Gln Met Gln Leu Ala Gln Ile Asp Gln Gln Ile Gln Gln Ala
245 250 255
Ala Thr Ser Leu Gly Pro Asn His Pro Thr Phe Gln Ala Leu Gln Arg
260 265 270
Gln Arg Glu Val Phe Ala Lys Ala Ala Ala Ala Glu Arg Ala Gln Ala
275 280 285
Asn Gly Val Ser Gly Pro Ala Arg Gly Ala Ile Glu Ser Ala Ala Asn
290 295 300
Ala Gln Arg Ala Arg Val Leu Gly Asn Arg Gln Asp Val Asp Lys Leu
305 310 315 320
Thr Gln Leu Gln Arg Asp Val Ser Leu Lys Gln Asp Gln Tyr Met Lys
325 330 335
Ala Ala Gln Arg Val Ala Asp Leu Arg Leu Glu Ala Ser Ser Asn Asp
340 345 350
Val Gly Met Ser Thr Leu Ser Glu Ala Ser Ala Pro Glu Thr Pro Tyr
355 360 365
Tyr Pro Lys Val Pro Leu Ile Ile Gly Gly Ala Ala Gly Phe Gly Leu
370 375 380
Gly Leu Gly Leu Leu Val Ala Leu Leu Val Glu Leu Leu Gly Arg Arg
385 390 395 400
Val Arg Ser Pro Glu Asp Leu Glu Val Ala Ile Asp Ala Pro Val Leu
405 410 415
Gly Val Ile Gln Ser Arg Ala Ser Leu Ala Ala Arg Leu Arg Arg Ala
420 425 430
Gln Glu Thr Leu Gly Glu Gly Ala Asp Thr His Gly Ala Ser Val Asn
435 440 445
<210>24
<211>708
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(708)
<223>dpsB
<400>24
atg gac gcg atg acc agc gaa ccg ctg ccc gaa ggc gat cgt ccg agc
48
Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser
1 5 10 15
gcc gtg ccg acc acg ccg gat acg atc ggc atg ctc gaa tac cag ctc
96
Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu
20 25 30
gtc ctc tcc gat ccg acc ggg atc gag gcg gaa gcg atc cgc gcg cta
144
Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu
35 40 45
cgc acg cgc atc atg acc cag cac ctc cgc gag ggc cgg cgc gcg ctc
192
Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu
50 55 60
gcg atc tgc gcc gcc tcg gcg gga tcc ggc tgc agc ttc acc gcc gtc
240
Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val
65 70 75 80
aat ctg gcg acg gcg ctg gcg cag atc ggc gtt aag act gcg ctg gtc
288
Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val
85 90 95
gat gcc aat ctg cgc gat ccc agc atc ggc gca gcc ttc ggc ctc gcc
336
Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala
100 105 110
gcc gac aag ccc ggc ctg gcc gat tat ctc gcc tcg ggc gat gtc gac
384
Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp
115 120 125
ctc gcc tcg atc atc cat gcg acc cgc ctc gac cag ctc tcg atc atc
432
Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile
130 135 140
ccg gcc ggg cat gtc gag cac agc ccg cag gaa ctg ctc gcg tcc gaa
480
Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu
145 150 155 160
cag ttc cat gat ctg gcg acg cag ctg ctg cgc gag ttc gac atc acg
528
Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr
165 170 175
atc ttc gac acc acg gcg tcc aac acc tgc gcc gac gcg cag cgt gtc
576
Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val
180 185 190
gcg cat atc gcc ggc tat gcg atc atc gtg gcg cgc aag gat gcg agc
624
Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser
195 200 205
tac atc cgc gac gtg aac acg ctc agc cgc acg ctg cgt gca gac cgc
672
Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg
210 215 220
acc aac gtc atc ggc tgc gta ctg aac ggc tat tga
708
Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr *
225 230 235
<210>25
<211>235
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的多糖输出蛋白
<400>25
Met Asp Ala Met Thr Ser Glu Pro Leu Pro Glu Gly Asp Arg Pro Ser
1 5 10 15
Ala Val Pro Thr Thr Pro Asp Thr Ile Gly Met Leu Glu Tyr Gln Leu
20 25 30
Val Leu Ser Asp Pro Thr Gly Ile Glu Ala Glu Ala Ile Arg Ala Leu
35 40 45
Arg Thr Arg Ile Met Thr Gln His Leu Arg Glu Gly Arg Arg Ala Leu
50 55 60
Ala Ile Cys Ala Ala Ser Ala Gly Ser Gly Cys Ser Phe Thr Ala Val
65 70 75 80
Asn Leu Ala Thr Ala Leu Ala Gln Ile Gly Val Lys Thr Ala Leu Val
85 90 95
Asp Ala Asn Leu Arg Asp Pro Ser Ile Gly Ala Ala Phe Gly Leu Ala
100 105 110
Ala Asp Lys Pro Gly Leu Ala Asp Tyr Leu Ala Ser Gly Asp Val Asp
115 120 125
Leu Ala Ser Ile Ile His Ala Thr Arg Leu Asp Gln Leu Ser Ile Ile
130 135 140
Pro Ala Gly His Val Glu His Ser Pro Gln Glu Leu Leu Ala Ser Glu
145 150 155 160
Gln Phe His Asp Leu Ala Thr Gln Leu Leu Arg Glu Phe Asp Ile Thr
165 170 175
Ile Phe Asp Thr Thr Ala Ser Asn Thr Cys Ala Asp Ala Gln Arg Val
180 185 190
Ala His Ile Ala Gly Tyr Ala Ile Ile Val Ala Arg Lys Asp Ala Ser
195 200 205
Tyr Ile Arg Asp Val Asn Thr Leu Ser Arg Thr Leu Arg Ala Asp Arg
210 215 220
Thr Asn Val Ile Gly Cys Val Leu Asn Gly Tyr
225 230 235
<210>26
<211>882
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(882)
<223>dpsM
<400>26
atg gca gcg acc gcg atg acg cgg cag cag gag agg aag ggc ggt ggc
48
Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly
1 5 10 15
tat tgg ctg gcc gtt gcc ggt ctt gcc gcg cta acc atc ccg acc ttc
96
Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe
20 25 30
atc acc ctg ggt cgc gag gtt tgg agt gcg gaa ggc ggc gtg cag ggt
144
Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly
35 40 45
ccg atc gtg ctc gcc acg ggc gcc tgg atg ctg gcc cgc cag tgc tcg
192
Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser
50 55 60
acg atc gag gcg cta cgc cgc ccc ggc agc gtg ctg ctc ggc gcg ctg
240
Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu
65 70 75 80
ttc ctg ctg gcg acg ctt gcc ttc tac acc gtt gga cgg gtg ttc gac
288
Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp
85 90 95
ttc atc agt gtc gaa acc ttc gga ctg gtc gcg acc tat ctg gtc gtc
336
Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val
100 105 110
gcc tat ctc tat ttc ggt gcc agg gtg ctc cgt gcc gcc tgg ttc ccg
384
Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro
115 120 125
gtg ctg tgg ctg ttc ttc ctg gtg ccg ccg ccc ggc tgg gcc gtc gac
432
Val Leu Trp Leu Phe Phe Leu Val Pro Pro Pro Gly Trp Ala Val Asp
130 135 140
cgc atc acc gca ccg ctc aag gag ttc gtc tcc tat gcg gca acg ggc
480
Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly
145 150 155 160
ctg ctt tcc tgg gtg gat tat ccg atc ctg cgc cag ggc gtg aca ctg
528
Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu
165 170 175
ttc gtc ggc ccc tat cag ctg ctc gtc gaa gat gcc tgt tcg ggt ctg
576
Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu
180 185 190
cgc tcg ctg tcc agc ctg gtc gtc gtg acg ctg ctc tac atc tac atc
624
Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile
195 200 205
aag aac aag ccg tcc tgg cgc tac gcg gcg ttc atc gca gcg ctg gtg
672
Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val
210 215 220
atc ccg gtg gca gtg gtg acc aac gtc ctg cgg atc atc atc ctg gta
720
Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val
225 230 235 240
ctg atc acc tat cat ctg ggc gac gag gcg gcg cag agc ttc ctc cac
768
Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His
245 250 255
gtc tcc acc ggc atg gtg atg ttc gtg gtc gcc ctg ctt tgc atc ttc
816
Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe
260 265 270
gcg atc gac tgg gtg gtc gag caa ctt ctt ctc ctg cgt cgg agg cat
864
Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His
275 280 285
cat gtt caa ccg gcg tga
882
His Val Gln Pro Ala *
290
<210>27
<211>293
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的多糖输出蛋白
<400>27
Met Ala Ala Thr Ala Met Thr Arg Gln Gln Glu Arg Lys Gly Gly Gly
1 5 10 15
Tyr Trp Leu Ala Val Ala Gly Leu Ala Ala Leu Thr Ile Pro Thr Phe
20 25 30
Ile Thr Leu Gly Arg Glu Val Trp Ser Ala Glu Gly Gly Val Gln Gly
35 40 45
Pro Ile Val Leu Ala Thr Gly Ala Trp Met Leu Ala Arg Gln Cys Ser
50 55 60
Thr Ile Glu Ala Leu Arg Arg Pro Gly Ser Val Leu Leu Gly Ala Leu
65 70 75 80
Phe Leu Leu Ala Thr Leu Ala Phe Tyr Thr Val Gly Arg Val Phe Asp
85 90 95
Phe Ile Ser Val Glu Thr Phe Gly Leu Val Ala Thr Tyr Leu Val Val
100 105 110
Ala Tyr Leu Tyr Phe Gly Ala Arg Val Leu Arg Ala Ala Trp Phe Pro
115 120 125
Val Leu Trp Leu Phe Phe Leu Val Pro Pro Pro Gly Trp Ala Val Asp
130 135 140
Arg Ile Thr Ala Pro Leu Lys Glu Phe Val Ser Tyr Ala Ala Thr Gly
145 150 155 160
Leu Leu Ser Trp Val Asp Tyr Pro Ile Leu Arg Gln Gly Val Thr Leu
165 170 175
Phe Val Gly Pro Tyr Gln Leu Leu Val Glu Asp Ala Cys Ser Gly Leu
180 185 190
Arg Ser Leu Ser Ser Leu Val Val Val Thr Leu Leu Tyr Ile Tyr Ile
195 200 205
Lys Asn Lys Pro Ser Trp Arg Tyr Ala Ala Phe Ile Ala Ala Leu Val
210 215 220
Ile Pro Val Ala Val Val Thr Asn Val Leu Arg Ile Ile Ile Leu Val
225 230 235 240
Leu Ile Thr Tyr His Leu Gly Asp Glu Ala Ala Gln Ser Phe Leu His
245 250 255
Val Ser Thr Gly Met Val Met Phe Val Val Ala Leu Leu Cys Ile Phe
260 265 270
Ala Ile Asp Trp Val Val Glu Gln Leu Leu Leu Leu Arg Arg Arg His
275 280 285
His Val Gln Pro Ala
290
<210>28
<211>699
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(699)
<223>dpsN
<400>28
atg ttc aac cgg cgt gac ctg ctg atc ggc gca ggc tgc ttc gcc gcc
48
Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala
1 5 10 15
gct ggc gcc tcg ctc ggc ctg aag ccg cac cgg cgg atg gac ctg ctg
96
Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu
20 25 30
ggc ggc acc aag ctc gac acg ctg atg ccc aag gca ttc ggc gca tgg
144
Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp
35 40 45
aag gca gag gat acc ggt tcg ctg atc gcg ccg gcg cgc gaa ggc agc
192
Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser
50 55 60
ctg gag gac aag ctc tac aac cag gtg gtc acc cgc gcc ttc tcc cgc
240
Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg
65 70 75 80
gcg gac ggt gcc caa gtg atg ctg ctg atc gcc tat ggc aac gcc cag
288
Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln
85 90 95
acc gat cta ctg cag ctg cac cgg ccg gaa ata tgc tac ccg ttc ttc
336
Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe
100 105 110
ggc ttc acc gtg gtg gaa agc cat gag cag acc atc ccg gtg acg ccg
384
Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro
115 120 125
cag gtg acg atc ccc ggt cgc gcg ctg acc gcc acc aac ttc aac cgc
432
Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg
130 135 140
acc gag cag atc ctc tac tgg acc cgc gtc ggc gaa tat ctg ccg cag
480
Thr Glu Gln Ile Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln
145 150 155 160
aac ggc aat cag cag atg ctc gcg cgg ctg aag agc cag gtc cag ggc
528
Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly
165 170 175
tgg atc gtc gac ggt gtg ctg gtg cgc atc tcg acg gtg acg ccc gag
576
Trp Ile Val Asp Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu
180 185 190
gcg gaa gat ggc ctg agc gcc aat ctc gat ttc gcg cgc gag ctg gtg
624
Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val
195 200 205
aag acg ctc gac ccg cgc gtg ctg cgc ccg ctg ctc ggg aac ggg ctc
672
Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu
210 215 220
aca cgg cag ctc ggt cac cag gtc tga
699
Thr Arg Gln Leu Gly His Gln Val *
225 230
<210>29
<211>232
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的多糖输出蛋白
<400>29
Met Phe Asn Arg Arg Asp Leu Leu Ile Gly Ala Gly Cys Phe Ala Ala
1 5 10 15
Ala Gly Ala Ser Leu Gly Leu Lys Pro His Arg Arg Met Asp Leu Leu
20 25 30
Gly Gly Thr Lys Leu Asp Thr Leu Met Pro Lys Ala Phe Gly Ala Trp
35 40 45
Lys Ala Glu Asp Thr Gly Ser Leu Ile Ala Pro Ala Arg Glu Gly Ser
50 55 60
Leu Glu Asp Lys Leu Tyr Asn Gln Val Val Thr Arg Ala Phe Ser Arg
65 70 75 80
Ala Asp Gly Ala Gln Val Met Leu Leu Ile Ala Tyr Gly Asn Ala Gln
85 90 95
Thr Asp Leu Leu Gln Leu His Arg Pro Glu Ile Cys Tyr Pro Phe Phe
100 105 110
Gly Phe Thr Val Val Glu Ser His Glu Gln Thr Ile Pro Val Thr Pro
115 120 125
Gln Val Thr Ile Pro Gly Arg Ala Leu Thr Ala Thr Asn Phe Asn Arg
130 135 140
Thr Glu Gln lle Leu Tyr Trp Thr Arg Val Gly Glu Tyr Leu Pro Gln
145 150 155 160
Asn Gly Asn Gln Gln Met Leu Ala Arg Leu Lys Ser Gln Val Gln Gly
165 170 175
Trp Ile Val Asp Gly Val Leu Val Arg Ile Ser Thr Val Thr Pro Glu
180 185 190
Ala Glu Asp Gly Leu Ser Ala Asn Leu Asp Phe Ala Arg Glu Leu Val
195 200 205
Lys Thr Leu Asp Pro Arg Val Leu Arg Pro Leu Leu Gly Asn Gly Leu
210 215 220
Thr Arg Gln Leu Gly His Gln Val
225 230
<210>30
<211>1395
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1395)
<223>atrD
<400>30
atg aac gcc gtt gtt ccg atg cgc cgc ggc ggc ccg ctc gcc cgc atg
48
Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met
1 5 10 15
cgc gat acc gtg ctg cct gcc cgc gtc gac gct tat gac acc gcc ttc
96
Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe
20 25 30
ctg cct gcc gcg ctg gag atc atc gag cgg ccg gtt tcg ccc acc gcg
144
Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala
35 40 45
cgg ctt acc gcc aag gtg atg ctg gcc ggg ctg gcg atc acc gcc gcc
192
Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala
50 55 60
tgg ctg gcg atc ggc aag gtc gaa gtc gtc gcg ccg acg cag ggg cgg
240
Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg
65 70 75 80
atc gcg ccg atc ggc gag acc aag atc gtc cag tcg ccc gaa tcg ggg
288
Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly
85 90 95
atc gtc cgc cgc atc ctg gtg ggc gag ggg cag aag gtc gcc aag ggc
336
Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly
100 105 110
cag gtg ctg atc acg ctc gac ccg acc gtg tcg tcg gcg gag gcg gca
384
Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala
115 120 125
cag gcg aag gtg gcg ctg ctc agc gcc cag ctc gac gcc gca cgc aac
432
Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn
130 135 140
cag gcg atc atc gac gcg ctg gac ggc agg ggc ttc cgc ttc gtc gcg
480
Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala
145 150 155 160
cct gcc gcc gcc agc ccg ggc gaa gtg gcg acg cat cgc ggc ctc gcc
528
Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala
165 170 175
cgc gcc cgg ctg ggc cag atc gag gcg gcg ctg gcc ggc ggc cgc tcc
576
Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser
180 185 190
gat cgc ggt gcc gcc gtc tcg gcc gcg gcc gag gcg cag gca cag gtg
624
Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val
195 200 205
cgg aag ctc gaa cag tcg ctg ccg ctg ctc gaa cag cag atc gcc gcg
672
Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala
210 215 220
aac gag acg atg gcc gcc aag ggc tat gtc tcg aag ctg cgc gtc gtg
720
Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val
225 230 235 240
gag atg cgt cgc cag ctg atc gcc gag cgg cag gac ctg acg gcg gcg
768
Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala
245 250 255
cgc gct acg ctc gcc aaa ctc ggc cag cag tcg ctg agc gtc tcc agc
816
Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser
260 265 270
ctg tcg gcc aag acg cgc gag gag gcg cgg gcg cag gtg ctg cag gat
864
Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp
275 280 285
ctg gtc aag gcg cag gac gag gtg cgt gcc cgc ggc gag gac gtc gcc
912
Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala
290 295 300
aag gcg aat ctg cgc agc tcg ttc cgc gaa ctg cgc gcg ccg gtg agc
960
Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser
305 310 315 320
ggt acc gtc tcg cag ctg cag gtc cac acc gaa ggc ggc gtg gtg gaa
1008
Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu
325 330 335
ggg gcc aag ccg ctc ctc agc ctg gtt ccc gac aat gcc cgg ctc gag
1056
Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu
340 345 350
gcc gag gtg atg gtc gac aac agc gac atc ggc ttc gtc cac atc ggc
1104
Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly
355 360 365
atg ccg gta aag gtg aag ctg cag gcc ttt ccc tat acc cgc tac ggc
1152
Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly
370 375 380
atg att ccc ggc acg gtg gcg ggc atc agc ccc gag gcg gtg cag atg
1200
Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met
385 390 395 400
aag gag aac cag ccg ccg gtc tac aag gcg cgg atc gcg ctg gcg cgc
1248
Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg
405 410 415
ggg tat gtg ctg gcc cat ggc gca cag gtg ccg ctg cgg ccg ggg atg
1296
Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met
420 425 430
ctc gcg agc gcg gac atc gtc acc ggc aag cga acc ctg ttc agc tat
1344
Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr
435 440 445
ctg gtg ggg ccc gtg ctc gag acg ggg agt gac gcg ctg cac gag cgg
1392
Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg
450 455 460
tga
1395
*
<210>31
<211>464
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的分泌蛋白
<400>31
Met Asn Ala Val Val Pro Met Arg Arg Gly Gly Pro Leu Ala Arg Met
1 5 10 15
Arg Asp Thr Val Leu Pro Ala Arg Val Asp Ala Tyr Asp Thr Ala Phe
20 25 30
Leu Pro Ala Ala Leu Glu Ile Ile Glu Arg Pro Val Ser Pro Thr Ala
35 40 45
Arg Leu Thr Ala Lys Val Met Leu Ala Gly Leu Ala Ile Thr Ala Ala
50 55 60
Trp Leu Ala Ile Gly Lys Val Glu Val Val Ala Pro Thr Gln Gly Arg
65 70 75 80
Ile Ala Pro Ile Gly Glu Thr Lys Ile Val Gln Ser Pro Glu Ser Gly
85 90 95
Ile Val Arg Arg Ile Leu Val Gly Glu Gly Gln Lys Val Ala Lys Gly
100 105 110
Gln Val Leu Ile Thr Leu Asp Pro Thr Val Ser Ser Ala Glu Ala Ala
115 120 125
Gln Ala Lys Val Ala Leu Leu Ser Ala Gln Leu Asp Ala Ala Arg Asn
130 135 140
Gln Ala Ile Ile Asp Ala Leu Asp Gly Arg Gly Phe Arg Phe Val Ala
145 150 155 160
Pro Ala Ala Ala Ser Pro Gly Glu Val Ala Thr His Arg Gly Leu Ala
165 170 175
Arg Ala Arg Leu Gly Gln Ile Glu Ala Ala Leu Ala Gly Gly Arg Ser
180 185 190
Asp Arg Gly Ala Ala Val Ser Ala Ala Ala Glu Ala Gln Ala Gln Val
195 200 205
Arg Lys Leu Glu Gln Ser Leu Pro Leu Leu Glu Gln Gln Ile Ala Ala
210 215 220
Asn Glu Thr Met Ala Ala Lys Gly Tyr Val Ser Lys Leu Arg Val Val
225 230 235 240
Glu Met Arg Arg Gln Leu Ile Ala Glu Arg Gln Asp Leu Thr Ala Ala
245 250 255
Arg Ala Thr Leu Ala Lys Leu Gly Gln Gln Ser Leu Ser Val Ser Ser
260 265 270
Leu Ser Ala Lys Thr Arg Glu Glu Ala Arg Ala Gln Val Leu Gln Asp
275 280 285
Leu Val Lys Ala Gln Asp Glu Val Arg Ala Arg Gly Glu Asp Val Ala
290 295 300
Lys Ala Asn Leu Arg Ser Ser Phe Arg Glu Leu Arg Ala Pro Val Ser
305 310 315 320
Gly Thr Val Ser Gln Leu Gln Val His Thr Glu Gly Gly Val Val Glu
325 330 335
Gly Ala Lys Pro Leu Leu Ser Leu Val Pro Asp Asn Ala Arg Leu Glu
340 345 350
Ala Glu Val Met Val Asp Asn Ser Asp Ile Gly Phe Val His Ile Gly
355 360 365
Met Pro Val Lys Val Lys Leu Gln Ala Phe Pro Tyr Thr Arg Tyr Gly
370 375 380
Met Ile Pro Gly Thr Val Ala Gly Ile Ser Pro Glu Ala Val Gln Met
385 390 395 400
Lys Glu Asn Gln Pro Pro Val Tyr Lys Ala Arg Ile Ala Leu Ala Arg
405 410 415
Gly Tyr Val Leu Ala His Gly Ala Gln Val Pro Leu Arg Pro Gly Met
420 425 430
Leu Ala Ser Ala Asp Ile Val Thr Gly Lys Arg Thr Leu Phe Ser Tyr
435 440 445
Leu Val Gly Pro Val Leu Glu Thr Gly Ser Asp Ala Leu His Glu Arg
450 455 460
<210>32
<211>2187
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas s.)ATCC53159
<220>
<221>CDS
<222>(1)...(2187)
<223>atrB
<400>32
atg aca cgc gac gaa atg cag gcc acc ctg cag agc gcg ctc gcg gcc
48
Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala
1 5 10 15
cat ggg gcg gcg gag cgc gag gcg gag ctg cgc gaa tcc gga ctg gtg
96
His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val
20 25 30
gcg ttg tcg ctg ctg ctc ggc gcg cac aac atc gcc arc acg ccc gaa
144
Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu
35 40 45
cag ctg cgc cac gcg ctg ggc cat gcc gag gcg gca agc gcc gac gac
192
Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp
50 55 60
ctg atc ctc ctg gcc aag cgc cag cag ggc gtg cgc gcc aag gcc gtc
240
Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val
65 70 75 80
gag gtg ccg cgc ggc gga ctc gcc cgc cag ccg ctg ccc gcg atc gcc
288
Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala
85 90 95
gac ggg ccc gaa ggc tgg ttc gtg atc ggc ggc ctg acc gaa cat ggc
336
Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly
100 105 110
gtg atc atc cag cgc ccg ggc cat gcc ccg gaa cag gtc gac cgg gac
384
Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp
115 120 125
gcg ctg gac gcg atc tgg tcc ggc gcg ctg gtg ctg ctc acc acc cgc
432
Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg
130 135 140
gcg gtg gcg gga cgg ccg ctg cgg ttc ggc ctc tcc tgg ttc acc gcg
480
Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala
145 150 155 160
cag ttc cgg cgc tat cgc acg ctg ttc ctc gag gtg ctc ggc atc acc
528
Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr
165 170 175
ctc gcg ctc aac ctg ctc ggc ctc gcc gcg ccg ctg ttg ttc cag agc
576
Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser
180 185 190
gtg atc gac aag gtg ctg atc cac aac agc atg agc acg ctg agc gtg
624
Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val
195 200 205
ctc gcc ttc gcc ttc ctg gcg gtt tcg gtg tgg gaa gtg gcg ctc ggc
672
Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly
210 215 220
tgg atc cgc acc cgc ctg ttc acc gag acg acg cag aag atc gac gtc
720
Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val
225 230 235 240
gag ctg ggt gcc cgg ctg ttc cac cac ctg ctg gcg ctg ccg ctc gcc
768
Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala
245 250 255
tat ttc gag aag cgc cgc gtg ggc gac acc gtc acc cgc gtc cgc cag
816
Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln
260 265 270
ctc gag acg atc cgc gaa ttc ctt acc agc gcc tcg ctg acg gtg atg
864
Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met
275 280 285
gtg gac ccg ctg ttc acc ttc gtg ttc ctc gcc gcg atg ctg ttc tac
912
Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr
290 295 300
tcg ccg atg ctc tcg ggc atc gtg ctc gtg tcg ctg atc gcc tat gcg
960
Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala
305 310 315 320
atc gta tcg ttc agc gtc gcc ggg ccg ctc cgc gcg cgg gtg gag gac
1008
Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp
325 330 335
aag ttc gag aag agc tcc gcc agc aac gcg ctg ctc gtc gag agc gtc
1056
Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val
340 345 350
tcg ggc atc cac acg atc aag gcg acc gcg gtc gag ccg cac tgg cag
1104
Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln
355 360 365
aat cgc tgg gag cgc cag ctc gcc gcc cat acc gcc gcg tcg cag cgg
1152
Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg
370 375 380
ctg atc aat acc gcc aac acc ggc agc cag gcg atc gag ctg atc tcg
1200
Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser
385 390 395 400
aag ctg agc ttc gcg gcg atc ctg ttc ttc ggc gcc aag gcg gtg atc
1248
Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile
405 410 415
ggc ggc gcg atg agc gta ggc gcg ctg gtg gcg ttc aac atg ttc gcc
1296
Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala
420 425 430
cag cgc gtg tcc ggg ccg gtg atc cgc atg gcg cag ctg tgg cag gat
1344
Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp
435 440 445
ttc cag cag gtg cgc atc tcg gtc gag cgg ctg ggc gac gtg ctc aac
1392
Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn
450 455 460
cat ccg gtg gaa ccg cgc ccg gcc tcg gcg gcg acg ctg ccg gtg ctg
1440
His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu
465 470 475 480
cgc ggt gcg att cgc ttc gag aat gtc agc ttc cgc tat gcc gag gac
1488
Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp
485 490 495
cag ccg ccg gtg ctg agc gac atc acg ctc gac att ccg gcg ggc acc
1536
Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr
500 505 510
tcg ctc ggc atc gtc ggt tcg tcg ggc tcg ggc aag tcg acg ctg gcc
1584
Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala
515 520 525
aag ctg ctc cag cgg ctc aac ctg ccg aat ctc ggc cgc gtg ctg gtc
1632
Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val
530 535 540
gac gag gtc gac gtg gcg cag ctc gat ccc gcc tgg ctg cgt cgc cag
1680
Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln
545 550 555 560
atc ggc gtc gtg ctg cag gag aat ctg ctg ttc agc cgc tcg atc cgc
1728
Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg
565 570 575
gag aac atc gcg ctc tcc aac ccc gcc atg ccg ttc gag aat gtc gtc
1776
Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val
580 585 590
gcg gcg gcg acg ctg gcc ggc gcg cat gat ttc atc ctg cgc cag ccg
1824
Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro
595 600 605
cgc ggc tat gac acc gag atc gtc gag cgc ggc gtc aat ctc tcc ggc
1872
Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly
610 615 620
ggc cag cgc cag cgg ctc gcc atc gcc cgc gcg ctc gtc ggc aat ccg
1920
Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro
625 630 635 640
cgc atc ctg gtg ttc gac gaa gcg acc tcg gcg ctc gat gcc gag agc
1968
Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser
645 650 655
gag gag ctg atc cag aac aac ctg cgc gcc atc tcg gcc ggc cgc acg
2016
Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr
660 665 670
ctc gtg gtg atc gcg cat cgc ctg agc gcg gtg cgc agc tgc gac cgg
2064
Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys AspArg
675 680 685
atc atc acg ctc gaa cag ggc cgc atc gtc gag agc ggc cga cac gac
2112
Ile Ile Thr Leu Glu Gln GlyArg Ile Val Glu Ser Gly Arg His Asp
690 695 700
gaa ttg ttg cgc ctg ggc ggc cgc tat gcc gac ctg cac cgc cgc cag
2160
Glu Leu Leu Arg Leu Gly Gly Arg TyrAla Asp Leu His Arg Arg Gln
705 710 715 720
ggc ggc tat ggg gag att gcc gca tga
2187
Gly Gly Tyr Gly Glu Ile Ala Ala *
725
<210>33
<211>728
<212>PRT
<213>鞘氨醇单孢菌(Sphingomoncassp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>推测的分泌蛋白
<400>33
Met Thr Arg Asp Glu Met Gln Ala Thr Leu Gln Ser Ala Leu Ala Ala
1 5 10 15
His Gly Ala Ala Glu Arg Glu Ala Glu Leu Arg Glu Ser Gly Leu Val
20 25 30
Ala Leu Ser Leu Leu Leu Gly Ala His Asn Ile Ala Ile Thr Pro Glu
35 40 45
Gln Leu Arg His Ala Leu Gly His Ala Glu Ala Ala Ser Ala Asp Asp
50 55 60
Leu Ile Leu Leu Ala Lys Arg Gln Gln Gly Val Arg Ala Lys Ala Val
65 70 75 80
Glu Val Pro Arg Gly Gly Leu Ala Arg Gln Pro Leu Pro Ala Ile Ala
85 90 95
Asp Gly Pro Glu Gly Trp Phe Val Ile Gly Gly Leu Thr Glu His Gly
100 105 110
Val Ile Ile Gln Arg Pro Gly His Ala Pro Glu Gln Val Asp Arg Asp
115 120 125
Ala Leu Asp Ala Ile Trp Ser Gly Ala Leu Val Leu Leu Thr Thr Arg
130 135 140
Ala Val Ala Gly Arg Pro Leu Arg Phe Gly Leu Ser Trp Phe Thr Ala
145 150 155 160
Gln Phe Arg Arg Tyr Arg Thr Leu Phe Leu Glu Val Leu Gly Ile Thr
165 170 175
Leu Ala Leu Asn Leu Leu Gly Leu Ala Ala Pro Leu Leu Phe Gln Ser
180 185 190
Val Ile Asp Lys Val Leu Ile His Asn Ser Met Ser Thr Leu Ser Val
195 200 205
Leu Ala Phe Ala Phe Leu Ala Val Ser Val Trp Glu Val Ala Leu Gly
210 215 220
Trp Ile Arg Thr Arg Leu Phe Thr Glu Thr Thr Gln Lys Ile Asp Val
225 230 235 240
Glu Leu Gly Ala Arg Leu Phe His His Leu Leu Ala Leu Pro Leu Ala
245 250 255
Tyr Phe Glu Lys Arg Arg Val Gly Asp Thr Val Thr Arg Val Arg Gln
260 265 270
Leu Glu Thr Ile Arg Glu Phe Leu Thr Ser Ala Ser Leu Thr Val Met
275 280 285
Val Asp Pro Leu Phe Thr Phe Val Phe Leu Ala Ala Met Leu Phe Tyr
290 295 300
Ser Pro Met Leu Ser Gly Ile Val Leu Val Ser Leu Ile Ala Tyr Ala
305 310 315 320
Ile Val Ser Phe Ser Val Ala Gly Pro Leu Arg Ala Arg Val Glu Asp
325 330 335
Lys Phe Glu Lys Ser Ser Ala Ser Asn Ala Leu Leu Val Glu Ser Val
340 345 350
Ser Gly Ile His Thr Ile Lys Ala Thr Ala Val Glu Pro His Trp Gln
355 360 365
Asn Arg Trp Glu Arg Gln Leu Ala Ala His Thr Ala Ala Ser Gln Arg
370 375 380
Leu Ile Asn Thr Ala Asn Thr Gly Ser Gln Ala Ile Glu Leu Ile Ser
385 390 395 400
Lys Leu Ser Phe Ala Ala Ile Leu Phe Phe Gly Ala Lys Ala Val Ile
405 410 415
Gly Gly Ala Met Ser Val Gly Ala Leu Val Ala Phe Asn Met Phe Ala
420 425 430
Gln Arg Val Ser Gly Pro Val Ile Arg Met Ala Gln Leu Trp Gln Asp
435 440 445
Phe Gln Gln Val Arg Ile Ser Val Glu Arg Leu Gly Asp Val Leu Asn
450 455 460
His Pro Val Glu Pro Arg Pro Ala Ser Ala Ala Thr Leu Pro Val Leu
465 470 475 480
Arg Gly Ala Ile Arg Phe Glu Asn Val Ser Phe Arg Tyr Ala Glu Asp
485 490 495
Gln Pro Pro Val Leu Ser Asp Ile Thr Leu Asp Ile Pro Ala Gly Thr
500 505 510
Ser Leu Gly Ile Val Gly Ser Ser Gly Ser Gly Lys Ser Thr Leu Ala
515 520 525
Lys Leu Leu Gln Arg Leu Asn Leu Pro Asn Leu Gly Arg Val Leu Val
530 535 540
Asp Glu Val Asp Val Ala Gln Leu Asp Pro Ala Trp Leu Arg Arg Gln
545 550 555 560
Ile Gly Val Val Leu Gln Glu Asn Leu Leu Phe Ser Arg Ser Ile Arg
565 570 575
Glu Asn Ile Ala Leu Ser Asn Pro Ala Met Pro Phe Glu Asn Val Val
580 585 590
Ala Ala Ala Thr Leu Ala Gly Ala His Asp Phe Ile Leu Arg Gln Pro
595 600 605
Arg Gly Tyr Asp Thr Glu Ile Val Glu Arg Gly Val Asn Leu Ser Gly
610 615 620
Gly Gln Arg Gln Arg Leu Ala Ile Ala Arg Ala Leu Val Gly Asn Pro
625 630 635 640
Arg Ile Leu Val Phe Asp Glu Ala Thr Ser Ala Leu Asp Ala Glu Ser
645 650 655
Glu Glu Leu Ile Gln Asn Asn Leu Arg Ala Ile Ser Ala Gly Arg Thr
660 665 670
Leu Val Val Ile Ala His Arg Leu Ser Ala Val Arg Ser Cys Asp Arg
675 680 685
Ile Ile Thr Leu Glu Gln Gly Arg Ile Val Glu Ser Gly Arg His Asp
690 695 700
Glu Leu Leu Arg Leu Gly Gly Arg Tyr Ala Asp Leu His Arg Arg Gln
705 710 715 720
Gly Gly Tyr Gly Glu Ile Ala Ala
725
<210>34
<211>1413
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1413)
<223>dpsB
<400>34
atg aac gct ttc gaa gca cag cgc gcc ttt gag gag caa ctt cgg gcg
48
Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala
1 5 10 15
cat tcc cgg gtt acg cca tct gcc gct ccc gtg tgg cgt cgc tcg acg
96
His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr
20 25 30
ctg cgg atg gtc ctc tat acc gag ttg ctg ctg ctg gac agt ctc tcg
144
Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser
35 40 45
atc ctg gcc gga ttc cac gtc gcg gcg ggc acg cgc gac ggc aac tgg
192
Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp
50 55 60
ctg tcg ctg gcg ggc atc aac gtc ggc gtc ttc ctg ctg ccg atc gct
240
Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala
65 70 75 80
ctc ggc acc gcg ctc gca agc ggc acc tac tcg ctg aac tgc ctg cgc
288
Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg
85 90 95
tac ccg gtc agc ggc gtg aag agc atc ttc tcg gca ttc ttc ttc tcg
336
Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser
100 105 110
atc ttc gtc gtc ctg ctc ggc agc tac ctg ctg acg gcc gag ctg ccg
384
Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro
115 120 125
ctg tcc cgc gtg cag ctg gcg gag ggc gcg atc ctc tcg ctg gtc ctc
432
Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu
130 135 140
ctg atg gtg ggc cgc ctg atg ttc cgc cgc cac gtc cgc gcg gtt acc
480
Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr
145 150 155 160
ggc ggc agg ctg ctc gac gaa ctg gtc atc atc gac ggc gtc tcg ctc
528
Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu
165 170 175
gac gtc gcg ggc aat gcg gtc gcg ctc gac gcg cgg atc atc aat ctc
576
Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu
180 185 190
tcg ccg aac ccg cgc gat ccg caa atg ctg cat cgc ctg ggc acc acc
624
Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr
195 200 205
gtg atc ggg ttc gac cgg gtg atc gtc gcc tgc acc aag gag cat cgc
672
Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg
210 215 220
gcg gtc tgg gcg ctg ctg ctc aag ggc atg aac atc aag ggc gag atc
720
Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile
225 230 235 240
ctc gtc ccc cag ttc aat gcg ctg ggc gcg atc ggc gtg gac gcc ttt
768
Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe
245 250 255
gac ggg aag gat acg ctg gtc gtc tcg cag ggc ccg ctc aac atg ccc
816
Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro
260 265 270
aac cgc gcg aag aag cgc gcg ctc gat ctc gcg atc acc gta ccg gcc
864
Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala
275 280 285
gtg ctc gcg ctg gcg ccg ctg atg atc ctg gtg gcg atc ctg atc aag
912
Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys
290 295 300
ctg gag agc ccg ggc ccg gtg ttg ttc gcg cag gat cgc gtc ggc cgc
960
Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg
305 310 315 320
ggc aac cgg ctg ttc aag atc atg aag ttc cgc tcg atg cgc gta acg
1008
Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr
325 330 335
ctg tgc gac gcg aac ggc aac gtc tcg gcc agc cgc gac gac gat cgc
1056
Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg
340 345 350
atc acc aag gtc ggc cgc ttc atc cgc aag acc agc atc gac gaa ctg
1104
Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu
355 360 365
ccg cag ctg ctg aac gtg ctg cgc ggc gac atg agc gtc gtc ggc ccg
1152
Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro
370 375 380
cgg ccg cat gcg ctg ggc tcg cgc gcc gcc gat cac ctg ttc tgg gaa
1200
Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu
385 390 395 400
atc gac gag cgc tac tgg cac cgc cac acg ctc aag ccg ggc atg acc
1248
Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr
405 410 415
ggt ctg gcc cag gtg cgc ggt ttc cgc ggg gcg acc gat cgc cgc gtc
1296
Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val
420 425 430
gat ctg acc aac cgg ctc cag gca gac atg gaa tat atc gac gga tgg
1344
Asp Leu Thr Asn Arg Leu Gln Ala Asp Met Glu Tyr Ile Asp Gly Trp
435 440 445
gat atc tgg cgc gat atc acg atc ctg ttc aag acg ctg cgg gtg atc
1392
Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile
450 455 460
gtg cat tcg aac gca ttc tga
1413
Val His Ser Asn Ala Phe *
465 470
<210>35
<211>470
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>葡糖基-异戊二烯基磷酸转移酶I
<400>35
Met Asn Ala Phe Glu Ala Gln Arg Ala Phe Glu Glu Gln Leu Arg Ala
1 5 10 15
His Ser Arg Val Thr Pro Ser Ala Ala Pro Val Trp Arg Arg Ser Thr
20 25 30
Leu Arg Met Val Leu Tyr Thr Glu Leu Leu Leu Leu Asp Ser Leu Ser
35 40 45
Ile Leu Ala Gly Phe His Val Ala Ala Gly Thr Arg Asp Gly Asn Trp
50 55 60
Leu Ser Leu Ala Gly Ile Asn Val Gly Val Phe Leu Leu Pro Ile Ala
65 70 75 80
Leu Gly Thr Ala Leu Ala Ser Gly Thr Tyr Ser Leu Asn Cys Leu Arg
85 90 95
Tyr Pro Val Ser Gly Val Lys Ser Ile Phe Ser Ala Phe Phe Phe Ser
100 105 110
Ile Phe Val Val Leu Leu Gly Ser Tyr Leu Leu Thr Ala Glu Leu Pro
115 120 125
Leu Ser Arg Val Gln Leu Ala Glu Gly Ala Ile Leu Ser Leu Val Leu
130 135 140
Leu Met Val Gly Arg Leu Met Phe Arg Arg His Val Arg Ala Val Thr
145 150 155 160
Gly Gly Arg Leu Leu Asp Glu Leu Val Ile Ile Asp Gly Val Ser Leu
165 170 175
Asp Val Ala Gly Asn Ala Val Ala Leu Asp Ala Arg Ile Ile Asn Leu
180 185 190
Ser Pro Asn Pro Arg Asp Pro Gln Met Leu His Arg Leu Gly Thr Thr
195 200 205
Val Ile Gly Phe Asp Arg Val Ile Val Ala Cys Thr Lys Glu His Arg
210 215 220
Ala Val Trp Ala Leu Leu Leu Lys Gly Met Asn Ile Lys Gly Glu Ile
225 230 235 240
Leu Val Pro Gln Phe Asn Ala Leu Gly Ala Ile Gly Val Asp Ala Phe
245 250 255
Asp Gly Lys Asp Thr Leu Val Val Ser Gln Gly Pro Leu Asn Met Pro
260 265 270
Asn Arg Ala Lys Lys Arg Ala Leu Asp Leu Ala Ile Thr Val Pro Ala
275 280 285
Val Leu Ala Leu Ala Pro Leu Met Ile Leu Val Ala Ile Leu Ile Lys
290 295 300
Leu Glu Ser Pro Gly Pro Val Leu Phe Ala Gln Asp Arg Val Gly Arg
305 310 315 320
Gly Asn Arg Leu Phe Lys Ile Met Lys Phe Arg Ser Met Arg Val Thr
325 330 335
Leu Cys Asp Ala Asn Gly Asn Val Ser Ala Ser Arg Asp Asp Asp Arg
340 345 350
Ile Thr Lys Val Gly Arg Phe Ile Arg Lys Thr Ser Ile Asp Glu Leu
355 360 365
Pro Gln Leu Leu Asn Val Leu Arg Gly Asp Met Ser Val Val Gly Pro
370 375 380
Arg Pro His Ala Leu Gly Ser Arg Ala Ala Asp His Leu Phe Trp Glu
385 390 395 400
Ile Asp Glu Arg Tyr Trp His Arg His Thr Leu Lys Pro Gly Met Thr
405 410 415
Gly Leu Ala Gln Val Arg Gly Phe Arg Gly Ala Thr Asp Arg Arg Val
420 425 430
Asp Leu Thr Asn Arg Leu Gin Ala Asp Met Glu Tyr Ile Asp Gly Trp
435 440 445
Asp Ile Trp Arg Asp Ile Thr Ile Leu Phe Lys Thr Leu Arg Val Ile
450 455 460
Val His Ser Asn Ala Phe
465 470
<210>36
<211>879
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(879)
<223>rmlA
<400>36
atg aag ggc atc atc ctt gcg ggg ggc agc ggg acg cgc ctg tac ccc
48
Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro
1 5 10 15
gca acg cta tcg atc tcg aag cag ctg ctt ccc gtc tat gac aag ccg
96
Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro
20 25 30
atg atc ttc tat ccg ctg tcg gtg ctg atg ctc acc ggc atc cgg gac
144
Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp
35 40 45
atc ctg att atc tcc acc ccg cgc gac ctg ccg atg ttc cag gcg ctg
192
Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu
50 55 60
ctg ggc gac ggc tcg gcc ttc ggc atc aac ctc agc tat gcc gag cag
240
Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln
65 70 75 80
ccc tcc ccc aac ggg ctg gcc gaa gcg ttc atc atc ggc gcg gat ttc
288
Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe
85 90 95
gtc ggc aac gat ccc agc gcg ctg atc ctg ggc gac aac atc tat cac
336
Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His
100 105 110
ggc gaa aag atg ggc gag cgc tgc cag gca gcc gca gcg cag gca gcg
384
Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala
115 120 125
cag ggc ggt gca aac gtc ttc gcc tat cat gtc gac gac ccc gag cgc
432
Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg
130 135 140
tac ggc gtg gtc gcg ttc gac ccg gag acg ggc gtc gcc acc agc gtc
480
Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val
145 150 155 160
gag gaa aag ccg gcc gag ccc aag tcc aac tgg gcg atc acc ggc ctg
528
Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu
165 170 175
tat ttc tac gac aag gac gtg gtc gac atc gcc aag tcg atc cag ccc
576
Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro
180 185 190
tcg gcg cgc ggc gaa ctc gag atc acc gac gtc aac cgc gtt tac atg
624
Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met
195 200 205
gag cgc ggc gac ctg cac atc acg cgc ctc ggc cgc ggc tat gcc tgg
672
Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp
210 215 220
ctc gac acc ggc acg cat gac agc ctg cac gaa gcc ggc tcg ttc gtt
720
Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val
225 230 235 240
cgc acg ctc gag cat cgg acg ggc gtg aag atc gcc tgc ccg gag gaa
768
Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu
245 250 255
atc gcc ttc gaa agc ggc tgg ctc ggc gcc gaa gac ctg ctc aag cgc
816
Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg
260 265 270
gcc gcc ggc ctc ggc aag acc ggc tat gcc gcc tat ctc cgc aag gtt
864
Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val
275 280 285
gcg acc gca gca tga
879
Ala Thr Ala Ala *
290
<210>37
<211>292
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>葡萄糖-1-磷酸胸苷基转移酶
<400>37
Met Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro
1 5 10 15
Ala Thr Leu Ser Ile Ser Lys Gln Leu Leu Pro Val Tyr Asp Lys Pro
20 25 30
Met Ile Phe Tyr Pro Leu Ser Val Leu Met Leu Thr Gly Ile Arg Asp
35 40 45
Ile Leu Ile Ile Ser Thr Pro Arg Asp Leu Pro Met Phe Gln Ala Leu
50 55 60
Leu Gly Asp Gly Ser Ala Phe Gly Ile Asn Leu Ser Tyr Ala Glu Gln
65 70 75 80
Pro Ser Pro Asn Gly Leu Ala Glu Ala Phe Ile Ile Gly Ala Asp Phe
85 90 95
Val Gly Asn Asp Pro Ser Ala Leu Ile Leu Gly Asp Asn Ile Tyr His
100 105 110
Gly Glu Lys Met Gly Glu Arg Cys Gln Ala Ala Ala Ala Gln Ala Ala
115 120 125
Gln Gly Gly Ala Asn Val Phe Ala Tyr His Val Asp Asp Pro Glu Arg
130 135 140
Tyr Gly Val Val Ala Phe Asp Pro Glu Thr Gly Val Ala Thr Ser Val
145 150 155 160
Glu Glu Lys Pro Ala Glu Pro Lys Ser Asn Trp Ala Ile Thr Gly Leu
165 170 175
Tyr Phe Tyr Asp Lys Asp Val Val Asp Ile Ala Lys Ser Ile Gln Pro
180 185 190
Ser Ala Arg Gly Glu Leu Glu Ile Thr Asp Val Asn Arg Val Tyr Met
195 200 205
Glu Arg Gly Asp Leu His Ile Thr Arg Leu Gly Arg Gly Tyr Ala Trp
210 215 220
Leu Asp Thr Gly Thr His Asp Ser Leu His Glu Ala Gly Ser Phe Val
225 230 235 240
Arg Thr Leu Glu His Arg Thr Gly Val Lys Ile Ala Cys Pro Glu Glu
245 250 255
Ile Ala Phe Glu Ser Gly Trp Leu Gly Ala Glu Asp Leu Leu Lys Arg
260 265 270
Ala Ala Gly Leu Gly Lys Thr Gly Tyr Ala Ala Tyr Leu Arg Lys Val
275 280 285
Ala Thr Ala Ala
290
<210>38
<211>567
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(567)
<223>rmlC
<400>38
atg acc cag gtc cat cat cac gaa ctg tcc ggc gtc atc gag ttc acg
48
Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr
1 5 10 15
ccg ccc aaa tat ggc gac cac cgc ggc ttc ttc tcc gaa gtg ttc aag
96
Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys
20 25 30
cag tcg gtg ctc gat gcc gaa ggc gtc gag gca cgc tgg gtg cag gac
144
Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp
35 40 45
aat cag agc ttc tcg gcg gcc ccg ggc acg atc cgc ggc ctg cat ctc
192
Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu
50 55 60
cag gcg ccg ccc ttc gcc cag gcc aag ctg gtc cgc gtg ttg cgc ggc
240
Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly
65 70 75 80
gcg atc ttc gac gtc gcg gtc gac atc cgt cgc ggc tcg ccc acc tat
288
Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr
85 90 95
ggc aaa tgg gtc ggc gtc gag ctc tcg gcc gag aag tgg aac cag ctg
336
Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu
100 105 110
ctg gtc ccc gcc ggc tat gcg cac ggc ttc atg acg ctc gtt ccg gat
384
Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp
115 120 125
tgc gag atc ctc tac aag gtc agc gcc aaa tat tcg aag gat tcg gag
432
Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu
130 135 140
atg gcg atc cgt tgg gac gat ccc gat ctc gcc atc gcc tgg ccg gac
480
Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp
145 150 155 160
atc ggc gtc gag ccg gtc ctc tcc gaa aag gac gcg gtc gcc acg ccc
528
Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro
165 170 175
ttc gcc gaa ttc aac acc ccc ttc ttc tat cag ggc tga
567
Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly *
180 185
<210>39
<211>188
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶
<400>39
Met Thr Gln Val His His His Glu Leu Ser Gly Val Ile Glu Phe Thr
1 5 10 15
Pro Pro Lys Tyr Gly Asp His Arg Gly Phe Phe Ser Glu Val Phe Lys
20 25 30
Gln Ser Val Leu Asp Ala Glu Gly Val Glu Ala Arg Trp Val Gln Asp
35 40 45
Asn Gln Ser Phe Ser Ala Ala Pro Gly Thr Ile Arg Gly Leu His Leu
50 55 60
Gln Ala Pro Pro Phe Ala Gln Ala Lys Leu Val Arg Val Leu Arg Gly
65 70 75 80
Ala Ile Phe Asp Val Ala Val Asp Ile Arg Arg Gly Ser Pro Thr Tyr
85 90 95
Gly Lys Trp Val Gly Val Glu Leu Ser Ala Glu Lys Trp Asn Gln Leu
100 105 110
Leu Val Pro Ala Gly Tyr Ala His Gly Phe Met Thr Leu Val Pro Asp
115 120 125
Cys Glu Ile Leu Tyr Lys Val Ser Ala Lys Tyr Ser Lys Asp Ser Glu
130 135 140
Met Ala Ile Arg Trp Asp Asp Pro Asp Leu Ala Ile Ala Trp Pro Asp
145 150 155 160
Ile Gly Val Glu Pro Val Leu Ser Glu Lys Asp Ala Val Ala Thr Pro
165 170 175
Phe Ala Glu Phe Asn Thr Pro Phe Phe Tyr Gln Gly
180 185
<210>40
<211>1062
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(1062)
<223>rmlB
<400>40
atg cag cag acc ttc ctc gtc acc ggc ggc gcc ggc ttc atc ggc tcg
48
Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser
1 5 10 15
gcg gtg gtg cgc cac ctc gtc cgc cag ggc gcg cgc gtc atc aat ctc
96
Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu
20 25 30
gac aag ctc acc tat gcc ggc aac ccg gcc tcg ctg act gcg atc gag
144
Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu
35 40 45
aac gcg ccc aac tat cgc ttc gtc cat gcc gac atc gcc gac acc gcg
192
Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala
50 55 60
acg atc cta ccg ctg ctg cgc gag gag cag gtc gat gtg gtg atg cac
240
Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His
65 70 75 80
ctc gcc gcc gag agc cat gtc gat cgc tcg atc gac ggc cct ggc gag
288
Leu Ala Ala Glu Ser His Val Asp Arg Ser Ile Asp Gly Pro Gly Glu
85 90 95
ttc atc gag acc aat gtc gtc ggc acc ttc aag ctg ctc cag tcg gcg
336
Phe Ile Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala
100 105 110
ctg caa tat tgg cgc gag ctg gag ggc gag aaa cgc gac gcg ttc cgc
384
Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg
115 120 125
ttc cac cac atc tcc acc gac gaa gtg ttc ggc gac ctg ccg ttc gac
432
Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp
130 135 140
agc ggc atc ttc acc gaa gag acg ccc tat gat ccc tcc tcg ccc tat
480
Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr
145 150 155 160
tcg gcg tcg aag gcg gcg agc gac cat ctg gtg cgc gcc tgg ggc cac
528
Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His
165 170 175
acc tat ggc ctg ccg gtg gtg ctg tcg aac tgc tcg aac aat tac ggg
576
Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly
180 185 190
ccg ttc cac ttc ccc gag aag ctg atc ccg ttg acc atc ctc aac gcg
624
Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala
195 200 205
ctc gag ggc aag ccg ctg ccg gtc tac ggc aag ggc gag aat atc cgc
672
Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg
210 215 220
gac tgg ctg tat gtc gac gat cac gcc aag gcg ctg gcg acc atc gcc
720
Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala
225 230 235 240
acc acc ggc aag gtc ggc cag agc tac aat gtc ggc ggc cgc aac gag
768
Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu
245 250 255
cgg acc aac ctg cag gtg gtc gag acg atc tgc gac ctg ctc gac cag
816
Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln
260 265 270
cgc att ccg ctg gcc gac ggt cgc aag cgc cgc gaa ctg atc acc ttc
864
Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe
275 280 285
gtc acc gat cgc ccc ggc cat gac cgc cgc tac gcg atc gac gcg acc
912
Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr
290 295 300
aag ctc gag acc gag ctg ggc tgg aag gct gag gag aat ttc gac acc
960
Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr
305 310 315 320
ggc atc gcc gcg acg atc gac tgg tat ctg gcg aac gag tgg tgg tgg
1008
Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp
325 330 335
ggc ccg atc cgc tcc ggc aaa tat gcc ggc gag cgg ctg ggg cag acc
1056
Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr
340 345 350
gcc tga
1062
Ala *
<210>41
<211>353
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>dTDP-D-葡萄糖-4，6-脱水酶
<400>41
Met Gln Gln Thr Phe Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ser
1 5 10 15
Ala Val Val Arg His Leu Val Arg Gln Gly Ala Arg Val Ile Asn Leu
20 25 30
Asp Lys Leu Thr Tyr Ala Gly Asn Pro Ala Ser Leu Thr Ala Ile Glu
35 40 45
Asn Ala Pro Asn Tyr Arg Phe Val His Ala Asp Ile Ala Asp Thr Ala
50 55 60
Thr Ile Leu Pro Leu Leu Arg Glu Glu Gln Val Asp Val Val Met His
65 70 75 80
Leu Ala Ala Glu Ser His Val Asp Arg Ser lle Asp Gly Pro Gly Glu
85 90 95
Phe lle Glu Thr Asn Val Val Gly Thr Phe Lys Leu Leu Gln Ser Ala
100 105 110
Leu Gln Tyr Trp Arg Glu Leu Glu Gly Glu Lys Arg Asp Ala Phe Arg
115 120 125
Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Pro Phe Asp
130 135 140
Ser Gly Ile Phe Thr Glu Glu Thr Pro Tyr Asp Pro Ser Ser Pro Tyr
145 150 155 160
Ser Ala Ser Lys Ala Ala Ser Asp His Leu Val Arg Ala Trp Gly His
165 170 175
Thr Tyr Gly Leu Pro Val Val Leu Ser Asn Cys Ser Asn Asn Tyr Gly
180 185 190
Pro Phe His Phe Pro Glu Lys Leu Ile Pro Leu Thr Ile Leu Asn Ala
195 200 205
Leu Glu Gly Lys Pro Leu Pro Val Tyr Gly Lys Gly Glu Asn Ile Arg
210 215 220
Asp Trp Leu Tyr Val Asp Asp His Ala Lys Ala Leu Ala Thr Ile Ala
225 230 235 240
Thr Thr Gly Lys Val Gly Gln Ser Tyr Asn Val Gly Gly Arg Asn Glu
245 250 255
Arg Thr Asn Leu Gln Val Val Glu Thr Ile Cys Asp Leu Leu Asp Gln
260 265 270
Arg Ile Pro Leu Ala Asp Gly Arg Lys Arg Arg Glu Leu Ile Thr Phe
275 280 285
Val Thr Asp Arg Pro Gly His Asp Arg Arg Tyr Ala Ile Asp Ala Thr
290 295 300
Lys Leu Glu Thr Glu Leu Gly Trp Lys Ala Glu Glu Asn Phe Asp Thr
305 310 315 320
Gly Ile Ala Ala Thr Ile Asp Trp Tyr Leu Ala Asn Glu Trp Trp Trp
325 330 335
Gly Pro Ile Arg Ser Gly Lys Tyr Ala Gly Glu Arg Leu Gly Gln Thr
340 345 350
Ala
<210>42
<211>867
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(867)
<223>rmlD
<400>42
atg cgt atc ctc gtc acc ggg cat gac ggc cag gtc gcc cag tcg ctg
48
Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu
1 5 10 15
gcc gag cag gcg gtg ggc cac gag ctg gtc ttc acc acc tac ccc gaa
96
Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu
20 25 30
ttc gat ctc tcc aag ccg gag acg atc gag gcc ggt gtg gcg cgg gtg
144
Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val
35 40 45
cac ccg gac ctg atc gtc tcc gcc gcc gcc tac acg gcg gtc gac aag
192
His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys
50 55 60
gcg gaa agc gaa ccc gag ctg gcg atg gcg atc aac ggc gac ggt ccc
240
Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro
65 70 75 80
ggc gtg ctg gcg cgc gcg ggc gcg aag atc ggc gcg ccg atc atc cac
288
Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His
85 90 95
ctg tcg acc gat tat gtg ttc gac ggc agt ctc gac cgc cct tgg cgc
336
Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg
100 105 110
gag gac gat ccc acc ggc ccg ctc ggc gtc tat ggc gcg acc aag ctg
384
Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu
115 120 125
gcc ggc gag cag gcg gtg cag gcc tcg ggt gcc acc aac gcc gtg atc
432
Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile
130 135 140
cgg ctg gcc tgg gtc tac agc ccg ttc ggc aac aat ttc gtc aag acg
480
Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr
145 150 155 160
atg ctc cgc ctc gcc gag acg cgc gac gcg ctg aac gtc gtg gag gac
528
Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp
165 170 175
cag tgg ggc tgc ccc agt tcg gcg ctg gac atc gcg acc gcg atc ctg
576
Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu
180 185 190
acg gtg gtc ggg cac tgg cag cag gac ggc gcg acg agc ggc ctc tac
624
Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr
195 200 205
cat ttc gcc ggc acc ggc gag acc aac tgg gcc gac ttc gca tcg acg
672
His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr
210 215 220
atc ttc gcc gag agc gcc aag cgc ggt ggc ccc tcg gcc acc gtc acc
720
Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr
225 230 235 240
ggc att ccc agc tcg ggc tat ccg act ccg gcc acg cgc ccg gcc aat
768
Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn
245 250 255
tcg cgg ctg gac tgc acc cgc ttc gcg gag acc ttc ggc tac cgg gcg
816
Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala
260 265 270
cct gcc tgg cag gat tcg ctg aac gtc gta ctg gat cgc ctg ctc ggc
864
Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly
275 280 285
tga
867
*
<210>43
<21l>288
<212>PRT
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>肽
<222>(0)...(0)
<223>dTDP-6-脱氧-L-甘露糖-脱氢酶
<400>43
Met Arg Ile Leu Val Thr Gly His Asp Gly Gln Val Ala Gln Ser Leu
1 5 10 15
Ala Glu Gln Ala Val Gly His Glu Leu Val Phe Thr Thr Tyr Pro Glu
20 25 30
Phe Asp Leu Ser Lys Pro Glu Thr Ile Glu Ala Gly Val Ala Arg Val
35 40 45
His Pro Asp Leu Ile Val Ser Ala Ala Ala Tyr Thr Ala Val Asp Lys
50 55 60
Ala Glu Ser Glu Pro Glu Leu Ala Met Ala Ile Asn Gly Asp Gly Pro
65 70 75 80
Gly Val Leu Ala Arg Ala Gly Ala Lys Ile Gly Ala Pro Ile Ile His
85 90 95
Leu Ser Thr Asp Tyr Val Phe Asp Gly Ser Leu Asp Arg Pro Trp Arg
100 105 110
Glu Asp Asp Pro Thr Gly Pro Leu Gly Val Tyr Gly Ala Thr Lys Leu
115 120 125
Ala Gly Glu Gln Ala Val Gln Ala Ser Gly Ala Thr Asn Ala Val Ile
130 135 140
Arg Leu Ala Trp Val Tyr Ser Pro Phe Gly Asn Asn Phe Val Lys Thr
145 150 155 160
Met Leu Arg Leu Ala Glu Thr Arg Asp Ala Leu Asn Val Val Glu Asp
165 170 175
Gln Trp Gly Cys Pro Ser Ser Ala Leu Asp Ile Ala Thr Ala Ile Leu
180 185 190
Thr Val Val Gly His Trp Gln Gln Asp Gly Ala Thr Ser Gly Leu Tyr
195 200 205
His Phe Ala Gly Thr Gly Glu Thr Asn Trp Ala Asp Phe Ala Ser Thr
210 215 220
Ile Phe Ala Glu Ser Ala Lys Arg Gly Gly Pro Ser Ala Thr Val Thr
225 230 235 240
Gly Ile Pro Ser Ser Gly Tyr Pro Thr Pro Ala Thr Arg Pro Ala Asn
245 250 255
Ser Arg Leu Asp Cys Thr Arg Phe Ala Glu Thr Phe Gly Tyr Arg Ala
260 265 270
Pro Ala Trp Gln Asp Ser Leu Asn Val Val Leu Asp Arg Leu Leu Gly
275 280 285
<210>44
<211>132
<212>DNA
<213>鞘氨醇单孢菌(Sphingomonas sp.)ATCC53159
<220>
<221>CDS
<222>(1)...(132)
<223>orf7(部分)，功能未知
<400>2
atc cgg ctg tgc ctg ggg tgc tgg cgg tcg ccc aag gaa atc gcc ggc 48
Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly
1 5 10 15
tgg agc gag ctg agt cct aag gga aag cgc gcg gtg cta gag gca ttg 96
Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu
20 25 30
ccg gcg cgc gaa cgg gag cat ggc ggg ggg cgc tga 132
Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg *
35 40
<210>45
<21l>43
<212>PRT
<213>鞘氨醇单孢菌(Spingomonas sp.)ATCC53159
<400>3
Ile Arg Leu Cys Leu Gly Cys Trp Arg Ser Pro Lys Glu Ile Ala Gly
1 5 10 15
Trp Ser Glu Leu Ser Pro Lys Gly Lys Arg Ala Val Leu Glu Ala Leu
20 25 30
Pro Ala Arg Glu Arg Glu His Gly Gly Gly Arg
35 40
Claims (24)
1.一种迪优坦胶，其显示固有粘度高于150 deciL/g，其中所述迪优坦胶是用鞘氨醇单孢菌ATCC 53159制得的，其含有质粒，所述质粒包含SEQ ID NO:1的核苷酸序列。
2.如权利要求1 所述的迪优坦胶，其特征在于，其显示固有粘度高于155 deciL/g。
3.如权利要求2 所述的迪优坦胶，其特征在于，其显示固有粘度高于160 deciL/g。
4.一种迪优坦胶，其显示海水3 rpm 粘度高于35 表盘读数；其中所述迪优坦胶是用鞘氨醇单孢菌ATCC 53159 制得的，其含有质粒，所述质粒包含SEQ ID NO:1的核苷酸序列。
5.如权利要求4 所述的迪优坦胶，其特征在于，其显示海水3 rpm 粘度高于37 表盘读数。
6.如权利要求5 所述的迪优坦胶，其特征在于，其显示海水3 rpm 粘度高于40 表盘读数。
7.如权利要求6 所述的迪优坦胶，其特征在于，其显示海水3 rpm 粘度高于42 表盘读数。
8.一种迪优坦胶，其显示海水0.3 rpm粘度高于35,000 cp，其中所述迪优坦胶是用鞘氨醇单孢菌ATCC 53159 制得的，其含有质粒，所述质粒包含SEQ ID NO:1的核苷酸序列。
9.如权利要求8 所述的迪优坦胶，其特征在于，其显示海水0.3 rpm粘度高于38,000 cp。
10.如权利要求9 所述的迪优坦胶，其特征在于，其显示海水0.3 rpm粘度高于40,000 cp。
11.如权利要求10 所述的迪优坦胶，其特征在于，其显示海水0.3 rpm粘度高于41,000 cp。
12.一种迪优坦胶，其显示在存在聚乙二醇分散剂时低剪切率粘度高于3500 cp，其中所述迪优坦胶是用鞘氨醇单孢菌ATCC 53159 制得的，其含有质粒，所述质粒包含SEQ ID NO:1的核苷酸序列。
13.如权利要求12 所述的迪优坦胶，其特征在于，其显示在存在聚乙二醇分散剂时低剪切率粘度高于3700 cp。
14.如权利要求13 所述的迪优坦胶，其特征在于，其显示在存在聚乙二醇分散剂时低剪切率粘度高于3900 cp。
15.如权利要求14 所述的迪优坦胶，其特征在于，其显示在存在聚乙二醇分散剂时低剪切率粘度高于4000 cp。
16.一种生产迪优坦胶的方法，所述方法包括：
将至少一种迪优坦生物合成酶的编码序列质粒，引入产生迪优坦的鞘氨醇单孢菌宿主微生物，所述宿主微生物是鞘氨醇单孢菌ATCC53159，所述质粒包含SEQ ID NO:1的核苷酸序列；
在发酵条件下培养该宿主微生物，从而使得该宿主微生物产生至少显示以下特征之一的迪优坦胶：
a) 固有粘度大于150 deciL/g；
b) 海水3 rpm 粘度高于35 表盘读数；
c) 海水0.3 rpm 粘度高于35,000 厘泊；和
d) 聚乙二醇分散剂存在时的低剪切率粘度高于3500 厘泊。
17.如权利要求16 所述的方法，其特征在于，所述质粒包含DpsG 聚合酶，所述DpSG 聚合酶由SEQ ID NO:1 的碱基2738-4894 编码。
18.如权利要求16 所述的方法，其特征在于，所述质粒包含：DpsG聚合酶，由SEQ ID NO:1 的碱基2738-4894 编码，和葡萄糖-1-磷酸胸苷基转移酶，由SEQ ID NO:1 的碱基22757-23631编码；dTDP-6-脱氧-葡萄糖-3-5-差向异构酶，由SEQ ID NO:1 的23632-24201 编码；dTDP-D-葡萄糖-4,6-脱水酶，由SEQ ID NO:1的24202-25262 编码；和dTDP-6-脱氧-L-甘露糖-脱氢酶，由SEQID NO:1 的25263-26276 编码。
19.如权利要求16 所述的方法，其特征在于，所述质粒包括：DpsG 聚合酶，由SEQ ID NO:1 的碱基2738-4894 编码和鼠李糖基转移酶IV，由SEQ ID NO:1 的碱基5093-7081 编码；葡糖基-异戊二烯基磷酸转移酶I，由SEQ ID NO:1 的碱基21229-22756编码；β-1,4-葡糖醛酸基转移酶II，由SEQ ID NO:1 的碱基7121-8163 编码；和葡糖基转移酶III，由SEQ ID NO:1的碱基8164-10466 编码。
20.如权利要求16 所述的方法，其特征在于，所述质粒包括：DpsG 聚合酶，由SEQ ID NO:1 的碱基2738-4894 编码和多糖输出蛋白DpsD，由SEQ ID NO:1 的碱基12389-13340 编码、DpsC，由SEQ ID NO:1 的碱基13341-14687 编码和DpsE，由SEQ ID NO:1 的碱基14687-15404编码。
21.如权利要求16 所述的方法，其特征在于，所述质粒包括：鼠李糖基转移酶IV，由SEQ ID NO:1 的碱基5093-7081 编码；β-1,4-葡糖醛酸基转移酶II，由SEQ ID NO:1 的碱基7121-8163编码；葡糖基转移酶III，由SEQ ID NO:1 的碱基8164-10466编码；葡萄糖-1-磷酸胸苷基转移酶，由SEQ ID NO:1的碱基22757-23631编码；葡糖基-异戊二烯基磷酸转移酶I，由SEQ ID NO:1 的碱基21229-22756编码；dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶，由SEQ ID NO:1 的碱基23632-24601编码； dTDP-D-葡萄糖-4,6-脱水酶，由SEQ ID NO:1 的碱基24202-25262 编码；和dTDP-6-脱氧-L-甘露糖-脱氢酶，由SEQ ID NO:1 的碱基25263-26129编码。
22.如权利要求16 所述的方法，其特征在于，所述质粒包含选自：DpsG 聚合酶，由SEQ ID NO:4编码; SEQ ID NO:7的推测的裂合酶；鼠李糖基转移酶IV，由SEQ ID NO:1 的碱基5093-7081 编码；β-1,4-葡糖醛酸基转移酶II，由SEQ ID NO:1 的碱基7121-8163 编码；葡糖基转移酶III，由SEQ ID NO:1 的碱基8164-10466 编码；多糖输出蛋白DpsD，由SEQ ID NO:1 的碱基12389-13340 编码、DpsC，由SEQ ID NO:1 的碱基13341-14687 编码和DpsE，由SEQ ID NO:1 的碱基14687-15404 编码；分泌蛋白，由SEQ ID NO:1 的碱基18454-20636 或20637-21228 编码；葡糖基-异戊二烯基磷酸转移酶I，由SEQ ID NO:1 的碱基21229-22756 编码；葡萄糖-1-磷酸胸苷基转移酶，由SEQ ID NO:1 的碱基22757-23631 编码；dTDP-6-脱氧-D-葡萄糖-3-5-差向异构酶，由SEQ ID NO:1 的碱基23632-24601编码；dTDP-D-葡萄糖-4,6-脱水酶，由SEQ ID NO:1 的碱基24202-25262 编码；和dTDP-6-脱氧-L-甘露糖-脱氢酶，由SEQ ID NO:1 的碱基25263-26129 编码；或其组合的迪优坦生物合成酶。
23.如权利要求16 所述的方法，其特征在于，将SEQ ID NO:1 的核酸序列引入产生迪优坦的鞘氨醇单孢菌宿主微生物。
24.一种分离的核酸分子，其核酸序列是SEQ ID NO:1 。
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| ZA200804213B (en) | 2022-03-30 |
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| AU2006308904B2 (en) | 2012-12-06 |
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| WO2007053612A3 (en) | 2007-12-06 |
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| AP2878A (en) | 2014-03-31 |
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