CN101548618A - Camptotheca acuminata decne cultivation method for improving camptothecine content - Google Patents
Camptotheca acuminata decne cultivation method for improving camptothecine content Download PDFInfo
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Abstract
Camptotheca acuminata decne cultivation method for improving camptothecine content relates to medicinal plant cultivation method. The technical scheme is steps of adding No.1 and No.2 accelerant to the roots in conventional camptotheca acuminata decne cultivation process, and the No.1 accelerant is granulated accelerant containing nitrogen, phosphorus, kalium, calcium, magnesium, sulphur, iron, boron, manganese, zinc, cuprum, molybdenum; the No.2 accelerant is granulated accelerant containing heavy metal salt, rare-earth and organic acid. The present invention is simple and convenient to implement without any instrument or establishment auxiliary; low cost; capable of improving biomass and camptothecine content of camptotheca acuminata decne fruit or branches and leaves rapidly significantly with high efficiency; being able to make good use of camptotheca acuminata decne resources, and protecting camptotheca acuminata decne resource with notable ecologic environment economic benefit.
Description
Technical field
The present invention relates to the cultivation method of medicinal plant, specifically is the cultivation method of camplotheca acuminata.
Background technology
Camplotheca acuminata (Camptotheca acuminata Decne) is that Nyssaceae (Nyssaceae) camplotheca acuminata belongs to (Camptotheca Decne.) plant, is distributed widely in the Asia, and it is especially many to distribute in China, is China's south endemic tree.Classify first national key protected wild plants (II level) through State Council approved in August, 1999.Camplotheca acuminata is the main natural origin of monoterpene indole alkaloid camptothecine.The present annual camptothecine demand in international market 3000kg, and available stock 1000kg only.
Camptothecine has active anticancer as the inhibitor of a kind of topoisomerase (Topoisor-merase), proves through clinical testing, and camptothecine all has obvious curative effects to kind of tumour surplus 30, and toxic and side effect is little, has high clinical value.And by camptothecine be raw material can prepare series derivative, as HCPT, TPT, Irinotecan, 9-AC (9-aminocamptothecin) and 9-NC (9-nitrocamptothecin) etc., all be that " topoisomerase enzyme inhibitor " is the extremely promising PTS of a class, the present camptothecine of producing all is by extracting in branches and leaves of camplotheca acuminata or the fruit, but the content of camptothecine is very little in camplotheca acuminata, (L ó pez-Meyer M such as L ó pez-Meyer, Nessler CL, McKnight TD (1994) Sites of accumulationof the antitumor alkaloid camptothecin in Camptotheca acuminata.Plantamedica 60:558-560.), (Liu Z such as Liu, Carpenter SB, Bourgeois WJ, etal. (1998) Variations in the secondary metabolite camptothecin inrelation to tissue age and season in Camptotheca acuminata.Tree Physiol18:265-270.) each position camptothecine content in the Camptotheca acuminata seedlings is analyzed, and find that the content in the camplotheca acuminata tender leaf is the highest, be about between 0.017% to 0.4%; But numerous documents show, in the tender leaf of various places self-sow camplotheca acuminata the content of camptothecine also only be about 0.1% (Zhang Yuhong, Zu YuanGang. camptothecine content is measured in the different places of production and the season of growth common camptotheca leaf. BULLETIN OF BOTANY Vol. .2003,20 (5): 572-575).Be to satisfy at present and following potential pharmaceutical drugs demand, improve in the camplotheca acuminata camptothecine content, become the focus of countries in the world scientist's concern.
For this reason, the inventor has carried out number of research projects to the camptothecine content that how to improve integral level in the camplotheca acuminata.
Summary of the invention
The purpose of this invention is to provide a kind of camptotheca acuminata decne cultivation method that improves camptothecine content.
The technical scheme that is adopted is in conventional method cultivation camplotheca acuminata to achieve the object of the present invention, be increased in root and execute a promoter and No. two promoter in the camptotheca acuminata decne cultivation planting process, said promoter being is contained the particle promoter of (in weight percent) nitrogen 5～15% and phosphorus 2～10% and potassium 5～10% and calcium 2～10% and magnesium 0.1～2% and sulphur 0.1～3% and iron 0.1～2% and boron 0.05～2% and manganese 0.1～2% and zinc 0.1～2% and copper 0.1～% and molybdenum 0.1～2%.Nitrogen, phosphorus, potassium, calcium, magnesium, sulphur, iron, boron, manganese, zinc, copper, molybdenum are to calculate with the amount of element in product, can make raw material and add composition granule fertilizer such as carclazyte, mountain earth, bentonite, anticaking agent with other compound that contains these elements when the preparation granule of product.
Said No. two promoter being are contained the particle promoter of (in weight percent) heavy metallic salt 25～40% and rare earth 15～30% and organic acid 8～12%, heavy metallic salt is meant copper sulphate or zinc sulphate, heavy metallic salt also can be with other slaine such as molysite, silver salt, replacements such as cadmium salt, rare earth is meant the rare earth chloride that contains lanthanum chloride and cerium chloride, also can use mixed rare earth carbonate, cerous nitrate, the cerous acetate organic acid is meant malonic acid or malic acid, also can replace with other organic acids such as maleic acids, other can add carclazyte, mountain earth, bentonite, anticaking agents etc. are made granule fertilizer.
Can be different for different camplotheca acuminata growing state cultivation methods, for the adult camplotheca acuminata that begins the result, preferred cultivation method is 2-3 month before picking fruit, is foundation with tree crown hat width of cloth diameter, every meter hat width of cloth is executed promoter 100-200g No. one, executes promoter 5-10g simultaneously No. two; Gathering leaf or fruit first half of the month then, every meter hat width of cloth is executed promoter 10-20g No. two.
For camplotheca acuminata childhood that does not begin the result as yet, preferred cultivation method is before the branches and leaves of gathering 1～2 month, is foundation with tree crown hat width of cloth diameter, and every meter hat width of cloth is executed promoter 50～100g No. one, executes promoter 5～10g simultaneously No. two; Gathering branches and leaves first half of the month then, and every meter hat width of cloth is executed promoter 10～20g No. two.
Treelet camplotheca acuminata for short close cultivation, preferred cultivation method is when seedling is more than 50 centimeters, every strain camplotheca acuminata is executed promoter 10～20g No. one, with No. two promoter 1～3g, after 2 months, every strain camplotheca acuminata is executed promoter 2～4g No. two, use two weeks after No. two promoter, overhead 20 centimeters the camplotheca acuminata branches and leaves of gathering with top, the back horse back of gathering executes promoter a 10～20g and No. two promoter 1～3g for every strain camplotheca acuminata, and after 2 months, every strain camplotheca acuminata is executed promoter 2～4g No. two, use one to two week after No. two promoter, the camplotheca acuminata branches and leaves of can gathering again.
The invention has the beneficial effects as follows:
The invention process is convenient and simple, does not need any apparatus or facility auxiliary; By present market price, prepare the 100 kilograms of about 120-150 of promoter cost of material units, prepare 100 kilograms of No. two promoter cost of material 400-450 units, with low cost; The fruit of camplotheca acuminata or the biomass and the camptothecine content of branches and leaves be can increase substantially fast, thereby the output of camptothecine, efficient height increased substantially; Can promote making full use of of camplotheca acuminata resource, protection camplotheca acuminata resource, ecotope remarkable in economical benefits.
Further set forth technical scheme of the present invention with embodiment below, but be not limited in embodiment.
Embodiment
The preparation of No. 1 promoter of embodiment (promoter of preparation 100kg)
Adopt following feedstock production:
1. urea CO (NH
2)
2(commercially available fertilizer contains N 46%)
2. superphosphate Ca (H
2PO
4)
2And CaSO
42H
2O (commercially available fertilizer contains P 16%, contains Ca 18%)
3. potassium chloride (KCl) (commercially available fertilizer contains K 60%)
4. magnesium sulfate MgSO
47H
2O (agricultural grade contains Mg 8.8%, contains S 13%)
5. zinc sulphate ZnSO
47H
2(agricultural grade contains Zn 21% to O, contains S 11%
6. copper sulphate CuSO
45H
2O (agricultural grade contains Cu 25%, contains S 22%)
7. ferrous sulfate FeSO
47H
2O (agricultural grade contains Fe 18%, contains S 12%)
8. boric acid H
3BO
3(agricultural grade contains B 18%)
9. manganese sulphate MnSO
4H
2O (technical grade contains Mn 32%, contains S 18%)
10. ammonium molybdate (NH
4)
2MoO
4(agricultural grade contains Mo 48%)
11. (agricultural grade contains SiO to bentonite
23.2%, AL
2O
311.4%, Fe
2O
30.29%, Na
2O 0.31%, and CaO 2.67%, and MgO 1.05%, K
2O 2.58%), bentonite have good adsorption,
Cohesiveness is convenient to the balling-up granulation, makes fertilizer efficiency slowly discharge, reduce simultaneously to run off and plays a part to improve the soil.
12. compound fertilizer anticaking agent (containing talcum powder, hydroscopic cellulose, odium stearate etc.) has the effect that prevents the material caking.
Prescription 1: take by weighing urea 25kg, superphosphate 35kg, potassium chloride 10kg, magnesium sulfate 4kg, zinc sulphate 3kg, copper sulphate 1.5kg, ferrous sulfate 1.5kg, boric acid 0.5kg, manganese sulphate 0.5kg, ammonium molybdate 0.5kg, bentonite 18kg, anticaking agent 0.5kg.
The material that weighs up is made promoter of particle by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.In the 100kg product there be the conversion constituent content: N 11.5%, P 5.6%, K 6.4%, Ca 6.8%, Mg 0.51%, S 1.6%, Fe 0.30%, B 0.09%, Mn 0.16%, Zn0.63%, Cu 0.37%, Mo 0.24%.
Prescription 2: take by weighing urea 35kg, superphosphate 25kg, potassium chloride 13kg, magnesium sulfate 6kg, zinc sulphate 4kg, copper sulphate 2.5kg, ferrous sulfate 2kg, boric acid 1kg, manganese sulphate 1kg, ammonium molybdate 1kg, bentonite 9kg, commercially available compound fertilizer anticaking agent 0.5kg.
The material that weighs up is made promoter of particle by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.In the 100kg product there be the conversion constituent content: N 16.1%, P 4%, K 8%, Ca 4.7%, Mg 0.62%, S 2.3%, Fe 0.38%, B 0.18%, Mn 0.32%, Zn0.84%, Cu 0.63%, Mo 0.48%.
Prescription 3: take by weighing urea 15kg, superphosphate 40kg, potassium chloride 15kg, magnesium sulfate 3kg, zinc sulphate 3kg, copper sulphate 3kg, ferrous sulfate 2kg, boric acid 1.5kg, manganese sulphate 1.5kg, ammonium molybdate 1.5kg, bentonite 15kg, commercially available compound fertilizer anticaking agent 0.5kg.
The material that weighs up is made particle promoter by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.Being converted to constituent content in the 100kg product is: N 6.9%, P 6.4%, K 9.4%, Ca 7.6%, Mg 0.42%, S 2.1%, Fe 0.55%, B 0.27%, Mn 0.48%, Zn0.63%, Cu 0.75%, Mo 0.72%
The preparation of No. two promoter (No. two promoter of preparation 100kg)
Adopt following raw material:
1. copper sulphate or zinc sulphate (agricultural grade, content is more than or equal to 98%)
2. (technical grade contains Ce 27% to rare earth chloride, and La 17%, and Ca 1.5%, and Mg 1.5%, and Ba 0.3%; Fe0.007%)
3. malonic acid or malic acid (technical grade, content is more than or equal to 99.5%)
4. bentonite (same promoter)
5. anticaking agent (same promoter)
Prescription 1: take by weighing copper sulphate 40kg, rare earth chloride 15kg, malonic acid 10kg, bentonite 34kg, anticaking agent 1kg.The material that weighs up is made particle promoter by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.
Prescription 2: take by weighing zinc sulphate 30kg, rare earth chloride 25kg, malic acid 12kg, bentonite 32kg, anticaking agent 1kg.The material that weighs up is made particle promoter by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.
Prescription 3: take by weighing zinc sulphate 25kg, rare earth chloride 30kg, malonic acid 8kg, bentonite 36kg, anticaking agent 1kg.The material that weighs up is made particle promoter by conventional method pulverizing, mixing, granulation, drying, cooling and method for sieving.
Embodiment 2 has begun result's adult camptotheca acuminata decne cultivation
The big tree of camplotheca acuminata is planted in Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, transplants in 1985, and the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 30 centimetres, and highly about 12 meters, about 8 meters of crown diameter, it is good to grow, and branches and leaves are luxuriant.
On August 1st, 2007, before picking fruit 3 months, the big tree of selected 6 identical growing way unanimities of the age of tree is divided into two groups (processed group and control groups) at random.Processed group is a foundation with tree crown hat width of cloth diameter, and every meter hat width of cloth is executed a promoter 100g of prescription 1, executes No. two promoter 10g of prescription 1 simultaneously; At picking fruit and branches and leaves first half of the month (on October 15th, 2007), every meter hat width of cloth is executed 1 No. two promoter 20g of prescription then.Control group is executed agricultural production respectively with fertile principal item Norway composite fertilizer (N, P2O5, K2O each 16%) 200g and 20g the same period by above standard.If do not rain at ordinary times, every day trickle once.On November 1st, 2007,6 trees all fruits of gathering respectively.Dry in the shade, pulverize the content that camptothecine is measured in the back.Extract and assay method with reference to Yan Xiufeng, etc. reported method, document " Yan Xiufeng, Wang Yang, in great waves, Zhang Yuhong, Yin Lijun. the efficient liquid phase chromatographic analysis analytical test journal of camptothecine content in the common camptotheca leaf, 2002,21 (2): 15-18 ".
Concrete detection method is as follows.
The extraction of camptothecine: accurately take by weighing 0.4 gram drying material, pour in 50 milliliters of conical flasks, add 25 milliliter 75% ethanol.After weighing, place Ultrasound Instrument, 50 ℃ were extracted 10 minutes.After being chilled to room temperature, refill the amount of liquid evaporation with 75% ethanol.0.45 micron filter membrane of this extract and 10 μ l are used for the high performance liquid chromatography (HPLC) of camptothecine and measure.
Efficient liquid phase chromatographic analysis: adopt high performance liquid chromatograph (Waters company, the U.S.), comprise high-pressure pump (Waters2695), a controller and a photodiode array detector (Waters2996).Chromatographic condition is: chromatographic column is used C-18 post (5 μ m, 4.6 * 250 millimeters), and wash-out flowing phase acetonitrile: water (30: 70), flow velocity are 1ml/min, and the detection wavelength is 254nm.Retention time is 11.8min.Adopt peak area to carry out quantitatively.The camptothecine standard items are available from Nat'l Pharmaceutical ﹠ Biological Products Control Institute.Sample repeats to average for three times.(following examples adopt the same detection method)
| Fruit dry weight (kg) | Camptothecine content (%/dry weight) | |
| Contrast tree 1 | 3.58 | 0.124±0.021 |
| Contrast tree 2 | 3.42 | 0.132±0.024 |
| Contrast tree 3 | 3.73 | 0.126±0.036 |
| Handle tree 1 | 4.53 | 0.195±0.047 |
| Handle tree 2 | 4.26 | 0.204±0.034 |
| Handle tree 3 | 4.37 | 0.196±0.026 |
Conclusion: the fruit harvesting amount is increased more than 20%, and the average camptothecine content of fruit increases more than 50%.
Embodiment 3 do not begin as yet the result young age camplotheca acuminata cultivation
Camplotheca acuminata treelet provenance is picked up from Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, plants in research and teaching district, Chinese herbal medicine specimen garden, Guangdong Province, field planting in 2005, the diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 4 centimetres, and highly about 5 meters, about 4 meters of crown diameter, it is good to grow, and branches and leaves are luxuriant.
On April 1st, 2008, before picking fruit 2 months, the treelet of selected 6 identical growing way unanimities of the age of tree is divided into two groups (processed group and control groups) at random.Processed group is a foundation with tree crown hat width of cloth diameter, and every meter hat width of cloth is executed a promoter 50g of prescription 2, executes No. two promoter 10g of prescription 2 simultaneously; At branches and leaves first half of the month (on May 15th, 2008) of gathering, every meter hat width of cloth is executed promoter 20g No. two then.Control group is executed agricultural production respectively with fertile principal item Norway composite fertilizer (N, P2O5, K2O each 16%) 50g and 20g the same period by above standard.If do not rain at ordinary times, every day trickle once.On June 1st, 2008, the new then long branches and leaves of gathering dried in the shade, and pulverize, and measured camptothecine content (three repetitions are averaged).
| New long shoot leaf dry weight (kg) | Camptothecine content (%/dry weight) | |
| Contrast tree 1 | 1.53 | 0.104±0.022 |
| Contrast tree 2 | 1.46 | 0.112±0.034 |
| Contrast tree 3 | 1.66 | 0.106±0.026 |
| Handle tree 1 | 1.87 | 0.155±0.043 |
| Handle tree 2 | 1.75 | 0.164±0.038 |
| Handle tree 3 | 1.93 | 0.166±0.037 |
Conclusion: the branches and leaves amount of gathering is increased more than 20%, and the average camptothecine content of branches and leaves increases more than 50%.
The cultivation of embodiment 4 short close children camplotheca acuminatas in age
The provenance of the camplotheca acuminata of short close cultivation is picked up from Guangzhou Tianhe District dragon's cave-stalactite cave Guangdong Institute of Traditional Chinese Medicine Chinese herbal medicine specimen garden, plants in Guangdong Institute of Traditional Chinese Medicine living collection planting experiment district.Adopt the mode seeding and seedling raising broadcast sowing in January, 2008, seeding quantity is every square of 20g, sets up little shed heat and moisture preserving with film, treat seed sprouting after, the two ends of little shed are opened, and water every day and once promote growth.Before the sprigging, planting site is turned in advance, made 6 of wide 120 centimetres, high 15 centimetres furrow, length is decided with the plot.Long during when seedling to 15 centimetres of left and right sides, by 80 * 80 centimetres specifications plantation, drench sufficient normal root water after the field planting, afterwards every day trickle once, regularly weed a garden, to guarantee well-grown.Processed group picked at random 3 furrow are handled, the promoter that application in two dressings is different: when seedling is more than 50 centimeters, every strain camplotheca acuminata is executed promoter 10g of prescription 3 and No. two promoter 1g of prescription 3, after 2 months, 3 No. two promoter 2g of prescription are executed in every strain, one to two week stay 20 centimeters overground parts of gathering on the ground after using No. two promoter.The back horse back of gathering is executed No. 3 promoter 10g of prescription, the promoter 1g that fills a prescription 3 No. two, and after 2 months, No. two promoter 2g of prescription 3 are executed in every strain, go round and begin again, and can use in the anniversary and gather.Other 3 furrow in contrast, when seedling was more than 50 centimeters, every strain camplotheca acuminata was executed agricultural production with fertile principal item Norway composite fertilizer (N, P2O5, K2O each 16%) 10g, other field management and collecting method, collecting time are identical.Norway composite fertilizer 10g is executed in the every strain in back of gathering at once.The branches and leaves of gathering dry in the shade, and pulverize, and measure camptothecine content (three times repeat to average).
| Each individual plant branches and leaves dry weight (kg) | Camptothecine content (%/dry weight) | |
| Contrast tree 1 | 0.051 | 0.117±0.025 |
| Contrast tree 2 | 0.058 | 0.129±0.037 |
| Contrast tree 3 | 0.062 | 0.116±0.023 |
| Handle tree 1 | 0.065 | 0.241±0.045 |
| Handle tree 2 | 0.061 | 0.267±0.056 |
| Handle tree 3 | 0.076 | 0.256±0.033 |
Conclusion: the individual plant branches and leaves amount of gathering is increased more than 30%, and the average camptothecine content of branches and leaves increases more than 100%.
Claims (4)
1, a kind of camptotheca acuminata decne cultivation method that improves camptothecine content is characterized in that: in conventional camptotheca acuminata decne cultivation planting process, be increased in the step that root is executed promoter and No. two promoter,
A said promoter is the granule that contains nitrogen 5～15% and phosphorus 2～10% and potassium 5～10% and calcium 2～10% and magnesium 0.1～2% and sulphur 0.1～3% and iron 0.1～2% and boron 0.05～2% and manganese 0.1～2% and zinc 0.1～2% and copper 0.1～2% and molybdenum 0.1-2% in weight percent;
Said No. two promoter are to contain heavy metallic salt 25～40% in weight percent, rare earth 15～30%, the granule of organic acid 8～12%, heavy metallic salt are meant copper sulphate or zinc sulphate, rare earth is meant the rare earth chloride that contains lanthanum chloride and cerium chloride, and organic acid is meant malonic acid or malic acid.
2, according to the camptotheca acuminata decne cultivation method of the said raising camptothecine content of claim 1, it is characterized in that for the adult camplotheca acuminata that begins the result, before picking fruit 2～3 months, with tree crown hat width of cloth diameter is foundation, every meter hat width of cloth is executed promoter 100～200g No. one, executes promoter 5～10g simultaneously No. two; In picking fruit first half of the month, every meter hat width of cloth is executed promoter 10～20g No. two then.
3, according to the camptotheca acuminata decne cultivation method of the said raising camptothecine content of claim 1, it is characterized in that not beginning as yet result's camplotheca acuminata childhood for camplotheca acuminata, before the branches and leaves of gathering 1～2 month, with tree crown hat width of cloth diameter is foundation, every meter hat width of cloth is executed promoter 50～100g No. one, executes promoter 5～10g simultaneously No. two; Gathering branches and leaves first half of the month then, and every meter hat width of cloth is executed promoter 10～20g No. two.
4, camptotheca acuminata decne cultivation method according to the said raising camptothecine content of claim 1, it is characterized in that treelet camplotheca acuminata for short close cultivation, when seedling long to more than 50 centimeters the time, every strain camplotheca acuminata is executed promoter 10～20g No. one, with No. two promoter 1～3g, after 2～3 months, every strain camplotheca acuminata is executed promoter 2～4g No. two, use one to two week after No. two promoter, overhead 20～30 centimeters the camplotheca acuminata branches and leaves of gathering with top, the back of gathering executes promoter a 10～20g and No. two promoter 1～3g for every strain camplotheca acuminata at once, after 2～3 months, every strain camplotheca acuminata is executed promoter 2～4g No. two, uses one to two week after No. two promoter, the camplotheca acuminata branches and leaves of can gathering again.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212549A (en) * | 2011-04-02 | 2011-10-12 | 上海师范大学 | Method for increasing camptothecin content through double key enzyme gene co-transformation |
| CN102212550A (en) * | 2011-04-02 | 2011-10-12 | 上海师范大学 | Method for increasing content of camptothecin through co-transformation of double genes of transcription factor ORCA3 (Octadecanoie-responsive Cantharanthus AP2-doman protein 3) and key enzyme G10H (Geraniol 10-hydroxylase) |
| CN102674975A (en) * | 2012-05-29 | 2012-09-19 | 浙江农林大学 | Nutrition protective agent capable of promoting content of camptothecin in camptotheca acuminata to increase |
| CN104686147A (en) * | 2015-02-16 | 2015-06-10 | 唐忠海 | Large-scale mappia pittosporoides seedling and cultivation method |
| CN105009923A (en) * | 2015-08-12 | 2015-11-04 | 南京林业大学 | Camptotheca acuminate hard wood cutting propagation method |
Family Cites Families (3)
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|---|---|---|---|---|
| CN1225163C (en) * | 2003-05-30 | 2005-11-02 | 陕西师范大学 | High-content camptothecine camplotheca acuminata callus culture method |
| CN1234264C (en) * | 2003-11-20 | 2006-01-04 | 东北林业大学 | Technology for culturing and collecting seeding of Camptotheca acuminata Decne |
| CN101243773B (en) * | 2008-03-14 | 2011-02-09 | 成都大学 | Induction method for Camptotheca acuminata Decne polyploid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102212549A (en) * | 2011-04-02 | 2011-10-12 | 上海师范大学 | Method for increasing camptothecin content through double key enzyme gene co-transformation |
| CN102212550A (en) * | 2011-04-02 | 2011-10-12 | 上海师范大学 | Method for increasing content of camptothecin through co-transformation of double genes of transcription factor ORCA3 (Octadecanoie-responsive Cantharanthus AP2-doman protein 3) and key enzyme G10H (Geraniol 10-hydroxylase) |
| CN102674975A (en) * | 2012-05-29 | 2012-09-19 | 浙江农林大学 | Nutrition protective agent capable of promoting content of camptothecin in camptotheca acuminata to increase |
| CN104686147A (en) * | 2015-02-16 | 2015-06-10 | 唐忠海 | Large-scale mappia pittosporoides seedling and cultivation method |
| CN105009923A (en) * | 2015-08-12 | 2015-11-04 | 南京林业大学 | Camptotheca acuminate hard wood cutting propagation method |
| CN105009923B (en) * | 2015-08-12 | 2017-08-01 | 南京林业大学 | A kind of camplotheca acuminata cuttage propagation method |
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| CN101548618B (en) | 2010-12-29 |
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