CN101507430A - Freezeproof foam disinfectant and preparation method thereof - Google Patents
Freezeproof foam disinfectant and preparation method thereof Download PDFInfo
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- CN101507430A CN101507430A CNA2009100812978A CN200910081297A CN101507430A CN 101507430 A CN101507430 A CN 101507430A CN A2009100812978 A CNA2009100812978 A CN A2009100812978A CN 200910081297 A CN200910081297 A CN 200910081297A CN 101507430 A CN101507430 A CN 101507430A
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- disinfectant
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Abstract
The invention relates to a foam disinfectant, in particular to an antifreezing foam disinfectant and a preparation method thereof. The antifreezing foam disinfectant is obtained from 30 to 55 weight portions of antifreeze agent, 4 to 8 weight portions of foaming agent, 0.3 to 1 weight portion of foam stabilizer and 5 to 10 weight portions of disinfectant, by taking the weight portion of the antifreeze agent as reference, adding water into the materials until the total weight is 100 weight portions and uniformly mixing the materials. The antifreezing foam disinfectant can be well adhered to the surface of a smooth object, and reduces the dosage of the disinfectant, and medicines are uniformly dispersed, so that the antifreezing foam disinfectant is particularly suitable for disinfection of the surface of the smooth object. The antifreezing foam disinfectant has good antifreezing effect, cannot be frozen at the antifreezing temperature of between 0 DEG C and 40 DEG C below zero according to different formulations, realizes the aim of the disinfection at a low temperature, and simultaneously reduces the corrosivity of the antifreezing foam disinfectant on metals.
Description
Technical field
The present invention relates to foamaseptic, particularly a kind of Freezeproof foam disinfectant and preparation method thereof.
Technical background
China's national boundary is very long, and the entry and exit port is numerous.The disinfectant that uses at the port, mainly contain pulvis, liquid, tablet, granule etc., though foamaseptic at home oneself have research report (referring to document: Zhu Dalan, Zhang Wenfu. the research of hydrogen peroxide foam disinfectant sterilization rule, [J]. Chinese Disinfection magazine, 2006; 23 (6): 489), but practical application seldom.And foamaseptic has its original advantage, can well be attached to shiny surface, and consumption is few, dispersion of medicine, and retention time length (referring to document: Zhu Dalan, Zhang Wenfu. the prescription screening study of hydrogen peroxide foam disinfectant, [J]. Chinese Disinfection magazine, 2006; 23 (5): 390), be suitable for the sterilization of port to vehicular traffic.Northern China climate in winter cold, especially northwest and northeast, as Heilungkiang, Inner Mongolia Autonomous Region, Xinjiang, winter is very long, and temperature is lower than and period of 0 ℃ reaches five to six months in 1 year.Low temperature has 2 points to the main influence of disinfection efficiency: the one, and effective disinfectant at normal temperatures, bactericidal effect when using at low temperatures can reduce greatly, even lost efficacy, usually solve this problem by the working concentration that strengthens disinfectant, and the disinfectant of use high concentration, both increase the cost of disinfectant, also increased the consumption of resource; The side effect of using the disinfectant of high concentration to produce simultaneously also increases thereupon, as the corrosion problems of disinfectant and residue problem etc., and the residual pollution that can strengthen environment of disinfectant.The 2nd, disinfectant is directed at freezing below 0 ℃ and uses inconvenience, needs the heating and melting disinfectant, has so just prolonged the time of disinfecting, thereby has influenced the speed that vehicular traffic is open to the custom.
Chlorine-containing disinfectant is a class disinfectant of present extensive use, as TCCA (Trichloroisocyanuric acid), chlorine bromine glycolylurea, bleaching powder, clorox, calcium hypochlorite etc.Chlorine-containing disinfectant has corrosivity to metal, and the concentration of 1000mg/L can be used for prophylactic disinfection under the normal temperature, but cryogenic conditions (below 5 ℃) working concentration need strengthen 2~3 times.Peroxide disinfectant high-efficiency broad spectrum, bactericidal action is fast, but the character instability has corrosivity to metal, and cryogenic conditions (below 5 ℃) working concentration need strengthen 1~2 times (referring to document: Liu Xizhen, Chen Chuntian, Li Dongli etc.; Peracetic acid cold sterilization experimental study [J]. Chinese Disinfection magazine, 2004; 21 (4): 327.).
Summary of the invention
The objective of the invention is to solve the cold sterilization problem, thereby a kind of convenient Freezeproof foam disinfectant that uses is provided.
A further object of the present invention provides a kind of preparation method of Freezeproof foam disinfectant.
Freezeproof foam disinfectant provided by the invention when solving cold sterilization problem (thimerosal antifreeze), can well utilize the sterilization advantage of foamaseptic again.The frothing capacity of Freezeproof foam disinfectant of the present invention is good, foam stabilization, and freeze proof temperature can not freezed between 0 ℃ to subzero 40 ℃.
The component of Freezeproof foam disinfectant of the present invention and component content are: contain antifreeze 30~55 weight portions in gross weight is the Freezeproof foam disinfectant of 100 weight portions, foaming agent 4~8 weight portions, foam stabiliser 0.3~1 weight portion, disinfectant 5~10 weight portions, surplus is a water; Weight portion with antifreeze is a benchmark.
The preparation method of Freezeproof foam disinfectant of the present invention: the weight portion with antifreeze is a benchmark, with antifreeze 30~55 weight portions, foaming agent 4~8 weight portions, foam stabiliser 0.3~1 weight portion, disinfectant 5~10 weight portions, after adding water to total amount and being 100 weight portion mixing and stirring, obtain Freezeproof foam disinfectant.
Described antifreeze is selected from least a in the group that methyl alcohol, ethanol, ethylene glycol, sodium chloride, dodecyl sodium sulfate etc. are formed.
Described antifreeze methyl alcohol, ethanol, ethylene glycol can significantly improve sterilizing ability under the peroxide disinfectant cryogenic conditions (referring to document: Chen Chuntian, Liu Xizhen, Li Dongli etc.; Peracetic acid and antifreezing agent are to Disinfection Effect influence observation, [J]. Chinese Disinfection magazine, 2005; 22 (1): 60.); Described sodium chloride, dodecyl sodium sulfate can significantly increase the sterilizing ability under the chlorine-containing disinfectant cryogenic conditions, have reduced the working concentration of disinfectant, have reached the corrosive effect of reduction disinfectant.
Described foaming agent is an ionic surfactant, is selected from least a in the group that dodecyl benzyl dimethyl ammonium chloride, two ten alkyl-dimethyl ammonium chlorides, lauryl sodium sulfate etc. are formed.
Described foam stabiliser is selected from least a in the group that Arabic jelly, sodium alginate, xanthans, laruyl alcohol, lauroyl diethanol amine etc. are formed.
Described disinfectant is selected from least a in the group that bleaching powder, clorox, chlorine dioxide, DCCNa, TCCA (Trichloroisocyanuric acid), hydrogen peroxide, Peracetic acid etc. are formed.
The foam stabilization of Freezeproof foam disinfectant of the present invention, anti-freezing property is strong, and bactericidal action is strong, and freeze proof temperature can reach subzero 30 degree, even 40 degrees below zero all can well be obtained sterilization and bactericidal effect.As get the 100ml Freezeproof foam disinfectant, the capacity of adding to is in the 500ml beaker, use magnetic stirring apparatus high-speed stirred (rotating speed 800~1200 commentaries on classics/min) 30 minutes, foam number is more than 300ml, and the foam stabilization time is that the half life period was at 30~40 minutes.Bactericidal effect is not subjected to low temperature effect, is in 2~4 ℃ in environmental temperature, acts on 20 minutes, and energy 100% is killed Escherichia coli, staphylococcus aureus, Candida albicans etc.
Freezeproof foam disinfectant of the present invention possesses:
1, the disinfectant formulation of extensive use at present has tablet, pulvis, aqua, fumicants etc., the foam formulation is used seldom, and Freezeproof foam disinfectant of the present invention can well be attached to the smooth object surface, reduced disinfectant consumption, dispersion of medicine is particularly suitable for the sterilization on smooth object surface.
2, Freezeproof foam of the present invention belongs to aerosol-type, can make the aerosol dispenser by propellant, and is easy to use.
3, the good freezing protection effect of Freezeproof foam of the present invention, according to different prescriptions, freeze proof temperature can not freezed between 0 ℃ to subzero 40 ℃, has realized the cryogenic conditions purpose of sterilization down, has reduced corrosion of metal simultaneously.
4, Freezeproof foam of the present invention (below 5 ℃) bactericidal effect under cryogenic conditions is not subjected to obviously to influence: antifreeze methyl alcohol, ethanol, ethylene glycol have synergistic effect to the sterilizing ability of peroxide disinfectant, and sodium chloride, dodecyl sodium sulfate have synergistic effect to the sterilizing ability of chlorine-containing disinfectant.Foaming agent is an ionic surfactant, and the used disinfectant of the present invention is had the Synergistic biocidal effect.
5, Freezeproof foam of the present invention has diversity: conventional disinfectant is single formulation, or is aqua, or is aerosol.And Freezeproof foam of the present invention can use as foaming agent, also can use as aqua, can also make emulsion class aerosol-type, by the propellant dispenser, and the diversity of preparation, except easy to use, range of application is wider.
Embodiment
Embodiment 1
Get dodecyl benzyl dimethyl ammonium chloride 48ml, gum Arabic 3g, hydrogen peroxide 51g, mass concentration is 95% ethanol 300ml, dodecyl sodium sulfate 5g, above-mentioned raw materials is mixed, add water and be settled to the 1000ml aqueous solution, be made into milky white liquid, there is not precipitation, get 100ml, the capacity of being added to is in the 500ml beaker, uses (the rotating speed 800-1200 commentaries on classics/min) 30 minutes of magnetic stirring apparatus high-speed stirred, foam volume is 350ml, foam stabilization, half foam life period is 30 minutes, bactericidal property is good, act on 20 minutes, energy 100% is killed Escherichia coli, staphylococcus aureus, Candida albicans.Antifreeze temperature-30 ℃.
Embodiment 2
The antifreezing effect test
Antifreeze, foaming agent, foam stabiliser, disinfectant are pressed table 1 different proportion to be mixed, add water and be settled to 1000ml, each prescription is got 100ml behind the mixing, is loaded in the reagent bottle, add a cover and tighten, be positioned over (Thermo scientific company product, model: 702Rel#4), start refrigerator in the ultra low temperature freezer, temperature descends gradually from room temperature, reduce to-20 ℃ ,-22 ℃ ,-24 ℃ ,-26 ℃ ... in the time of-40 ℃, observe disinfectant successively and freeze situation, write down and antifreezely the results are shown in Table 1.
The antifreeze temperature of several prescriptions of table 1 Freezeproof foam disinfectant
Prescription | Form | Antifreezing effect (freezing temperature) |
1 | Dodecyl benzyl dimethyl ammonium chloride 40ml, gum Arabic 3 grams, hydrogen peroxide 51 grams, sodium chloride 50 grams, dodecyl sodium sulfate 5 grams | -10℃ |
2 | Dodecyl benzyl dimethyl ammonium chloride 40ml, gum Arabic 3 grams, hydrogen peroxide 51 grams, ethanol 150ml, dodecyl sodium sulfate 5 grams | -12℃ |
3 | Two ten alkyl-dimethyl ammonium chloride 48ml, gum Arabic 3 grams, hydrogen peroxide 51 grams, ethanol 300ml, dodecyl sodium sulfate 5 grams | -20℃ |
4 | Two ten alkyl-dimethyl ammonium chloride 48ml, lauryl sodium sulfate 10 grams, gum Arabic 6 grams, oxidation-reduction potential water 51ml, ethanol 300ml, sodium chloride 50 grams, dodecyl sodium sulfate 5 grams | -28℃ |
5 | Two ten alkyl-dimethyl ammonium chloride 48ml, lauryl sodium sulfate 10 grams, gum Arabic 6 grams, calcium hypochlorite 51 grams, ethanol 300ml, sodium chloride 50 grams, dodecyl sodium sulfate 5 grams | -30℃ |
6 | Two ten alkyl-dimethyl ammonium chloride 30ml, lauryl sodium sulfate 10 grams, gum Arabic 6 grams, TCCA (Trichloroisocyanuric acid) 45 grams, ethanol 300ml, sodium chloride 50 grams, dodecyl sodium sulfate 10 grams | -32℃ |
7 | Two ten alkyl-dimethyl ammonium chloride 48ml, lauryl sodium sulfate 10 grams, lauroyl diethanol amine 5 grams, gum Arabic 3 grams, hydrogen peroxide 51 grams, ethanol 350ml, methyl alcohol 50ml, dodecyl sodium sulfate 15 grams | -36℃ |
8 | Two ten alkyl-dimethyl ammonium chloride 50ml, lauryl sodium sulfate 10 grams, lauroyl diethanol amine 3 grams, gum Arabic 6 grams, persulfuric acid complex salt 50 grams, ethanol 200ml, methyl alcohol 50ml, ethylene glycol 200ml, dodecyl sodium sulfate 15 grams | -40℃ |
Embodiment 3
The foaming measure of merit
Table 1 different proportion of antifreeze, foaming agent, foam stabiliser, disinfectant being pressed embodiment 2 mixes, add water and be settled to 100ml (component of prescription 1 to 8 and content are with the table 1 of embodiment 2), each prescription is got 100ml behind the mixing, the capacity of adding to is in the 500ml beaker, (foam number and foam stabilization time are observed in rotating speed 800-1200 commentaries on classics/min) 30 minutes to use the magnetic stirring apparatus high-speed stirred.Foaming effect and foam stability see Table 2.
The foaming effect of several prescriptions of table 2 Freezeproof foam disinfectant
Embodiment 4
The bactericidal effect test
1. bactericidal assay method (with reference to the 2.1.1.74 of Ministry of Public Health's " disinfection technology standard ")
During test, the 1.0ml bacteria suspension is added in the test tube, the antifreeze foamaseptic that adds 4.0ml then, mixing also starts stopwatch and picks up counting, act on 10 minutes, 15 minutes and 20 minutes the time, inhale the above-mentioned suspension that contains bacteria suspension and antifreeze foamaseptic of 0.5ml respectively to the test tube that 4.5ml neutralizer (neutralizer be contain mass concentration be that 0.3% lecithin, mass concentration are that 3% Tween 80 and mass concentration are the phosphate buffer of 0.5% sodium thiosulfate) is housed, mixing.In and after 10 minutes, inhaling the 1.0ml mixed liquor is inoculated in the aseptic plate, in plate, add 20ml nutrient agar (nutrient agar is through high-temperature sterilization and be cooled to 45 ℃), treat that nutrient agar is after solidifying on the plate, plate is placed in 37 ℃ of incubators cultivated bacterium number on the counting plate 48 hours.The antifreeze foamaseptic of control group physiologic saline for substitute, other step is identical with above-mentioned experimental group.Bacterium number on plate calculates and kills sterilization rate according to experimental group and control group, and test repeats 3 times.Formula is as follows:
X is a sterilizing rate, %; N
CBe the average clump count of control group, cfu/ml; N
SBe the average clump count of experimental group, cfu/ml.
2. experimental result
2.1 to colibacillary sterilization result
The component of 8 kinds of prescriptions of antifreeze foamaseptic and content are with embodiment 2, bactericidal assay has been carried out in requirement by Ministry of Public Health's " disinfection technology standard " 2002 editions, the result shows that the working concentration effect 10 minutes of antifreeze foamaseptic reaches more than 99.9% Escherichia coli, staphylococcus aureus, Candida albicans killing rate, meets the sterilization requirement.
Table 3: several prescriptions are to the colibacillary average sterilization rate that kills
2.2 sterilization result to staphylococcus aureus
The component of 8 kinds of prescriptions of antifreeze foamaseptic and content have carried out bactericidal assay with embodiment 2 by the requirement of Ministry of Public Health's " disinfection technology standard " 2002 editions, and the killing effect of staphylococcus aureus is seen Table 4, and the oidiomycetic bactericidal effect of white is seen Table 5.The result shows that the sterilizing rate that acts on 15 minutes reaches more than 99.9%, reaches the sterilization requirement of 2002 editions regulations of Ministry of Public Health's " disinfection technology standard ".
Table 4: several prescriptions are to the average sterilization rate that kills of staphylococcus aureus
Table 5: several prescriptions are to the oidiomycetic average sterilization rate that kills of white
Claims (6)
1. Freezeproof foam disinfectant is characterized in that: in being the Freezeproof foam disinfectant of 100 weight portions, gross weight contains antifreeze 30~55 weight portions, and foaming agent 4~8 weight portions, foam stabiliser 0.3~1 weight portion, disinfectant 5~10 weight portions, surplus is a water; Weight portion with antifreeze is a benchmark.
2. Freezeproof foam disinfectant according to claim 1 is characterized in that: described antifreeze is selected from least a in the group that methyl alcohol, ethanol, ethylene glycol, sodium chloride, dodecyl sodium sulfate forms.
3. Freezeproof foam disinfectant according to claim 1 is characterized in that: described foaming agent is selected from least a in the group that dodecyl benzyl dimethyl ammonium chloride, two ten alkyl-dimethyl ammonium chlorides, lauryl sodium sulfate form.
4. Freezeproof foam disinfectant according to claim 1 is characterized in that: described foam stabiliser is selected from least a in the group that Arabic jelly, sodium alginate, xanthans, laruyl alcohol, lauroyl diethanol amine formed.
5. Freezeproof foam disinfectant according to claim 1 is characterized in that: described disinfectant is selected from least a in the group that bleaching powder, clorox, chlorine dioxide, DCCNa, TCCA (Trichloroisocyanuric acid), hydrogen peroxide, Peracetic acid form.
6. preparation method according to any described Freezeproof foam disinfectant of claim 1~5, it is characterized in that: the weight portion with antifreeze is a benchmark, with antifreeze 30~55 weight portions, foaming agent 4~8 weight portions, foam stabiliser 0.3~1 weight portion, disinfectant 5~10 weight portions after adding water to total amount and being 100 weight portion mixing and stirring, obtain Freezeproof foam disinfectant.
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