CN101434661B - Lichee polysaccharide, and extracting method and use thereof - Google Patents
Lichee polysaccharide, and extracting method and use thereof Download PDFInfo
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- CN101434661B CN101434661B CN2008102198558A CN200810219855A CN101434661B CN 101434661 B CN101434661 B CN 101434661B CN 2008102198558 A CN2008102198558 A CN 2008102198558A CN 200810219855 A CN200810219855 A CN 200810219855A CN 101434661 B CN101434661 B CN 101434661B
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- polysaccharide
- lichee
- lychee
- fructus litchi
- lichee polysaccharide
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Abstract
The invention discloses a lychee polysaccharide, an extraction method thereof and an application thereof in the preparation of health food with immunity regulation function. The preparation method of the lychee polysaccharide includes the steps of: selecting crushed lychee powder, adding water of 10 times to 30 times of lychee powder weight, regulating pH value to 7.5 to 9.5, carrying out ultrasonic-microwave synergistic extraction for 5min to 15min, obtaining an extracted solvent, and again carrying out condensation, deposition and drying so as to obtain the lychee polysaccharide. The lychee polysaccharide obtained through the method has high product yield and purity, and is simple in extraction technology, short in necessary time and low in cost. The lychee polysaccharide is also used in heath food, which explores a new path for the deep processing of lychee.
Description
Technical field
The present invention relates to a kind of lichee polysaccharide, also relate to the extracting method of this lichee polysaccharide and have application in the protective foods of immunoregulation effect in preparation.
Background technology
Lichee mainly is distributed in the ground such as Guangdong, Guangxi, Fujian, Hainan and Taiwan of China, it is the fruit tree of South China's cultivated area maximum, for a long time, lichee is regarded as " treasure in the fruit " always because of having higher nutritive value and multiple nourishing beauty treatment effect, the Compendium of Material Medica record " often eat lichee and can mend brain and enhancing fitness; the treatment scrofula, beneficial spleen whets the appetite; Drying can invigorating premordial QI, can be used as puerpera and old weak person's tonic ".
At present, the extracting method of polysaccharide mainly contains and adds heat extraction, enzymolysis process, microwave extraction method, ultrasonic extraction and ultrafiltration process etc., and these extracting method respectively have quality, as employing hot water extraction lichee polysaccharides such as Li Xuehua, yield is 3.30%, adopts the traditional water formulation, and extraction time is long, energy consumption is big; Dong Zhou Yong etc. adopt family's microwave oven to extract lichee polysaccharide, and polysaccharide yield is 6.17%, is small-scale test, are difficult to extensive enforcement on producing.Therefore, there is very big technological improvement space in the extracting method of lichee polysaccharide, discloses a kind of extraction process of lichee polysaccharide as patent 03100544.6, comprise select materials, steps such as making beating, enzymolysis and filtration, time-consuming length, complex process.
Summary of the invention
The object of the present invention is to provide a kind of lichee polysaccharide and extracting method thereof.
The present invention also aims to provide this lichee polysaccharide to have application in the protective foods of immunoregulation effect in preparation.
Lichee polysaccharide provided by the invention is selected the Fructus Litchi powder of pulverizing for use, adds the water of its 10～30 times of weight, and regulating pH is 7.5～9.5, and is ultrasonic-microwave cooperating extraction 5～15min, gets extracting solution, again through concentrated, the dry lichee polysaccharide that gets of precipitation.
The extracting method of lichee polysaccharide provided by the invention is selected the Fructus Litchi powder of pulverizing for use, adds the water of its 10～30 times of weight, and regulating pH is 7.5～9.5, and is ultrasonic-microwave cooperating extraction 5～15min, gets extracting solution, again through concentrated, the dry lichee polysaccharide that gets of precipitation.
When ultrasonic-microwave cooperating extracted, volume containing the sample and ultrasonic power ratio were 1.0～2.0g/w, and volume containing the sample and microwave power ratio are 1.0～1.5g/w.
The extracting solution concentration process of said extracted method: with extracting liquid filtering, collect filtrate, the centrifugal 10～20min of 4000～6000rpm collects supernatant liquor, concentrates, and gets concentrated solution.
Described simmer down to supernatant concentration to 1/10～2/5 of original volume.
The precipitation drying process of said extracted method is: in concentrated solution, adds ethanol and carries out alcohol precipitation, take off layer precipitation, and the centrifugal 10～20min of 4000～6000rpm, collecting precipitation, vacuum-drying promptly gets lichee polysaccharide.
In precipitation drying process used alcoholic acid volume percent is 85～95%, is added into that the alcoholic acid volume percent is 70～80% in the concentrated solution.
The preparation method of Fructus Litchi powder of the present invention may further comprise the steps:
(1) selection fresh lichee raw material, oven dry removes peeling and nuclear, gets Fructus Litchi;
(2) get Fructus Litchi, the ethanolic soln that adds its 8～12 times of weight refluxed 6～10 hours;
(3) get Fructus Litchi after step (2) is handled, 70～85 ℃ of oven dry are pulverized.
The volume percent of ethanolic soln is 70～85% described in the step (2).
The lichee polysaccharide that the inventive method is extracted has application in the immunoregulation effect protective foods in preparation.
The lichee polysaccharide of the present invention preparation, capable of direct drinking, or be mixed with particle, powder, tablet, capsule or beverage etc. with one or more pharmaceutically acceptable carriers.
The present invention has following advantage:
(1) the present invention has carried out taking off low molecular saccharides processing to Fructus Litchi earlier, and take the response surface analysis method that processing condition have been carried out optimization, improved the yield and the purity of lichee polysaccharide, with this understanding, the productive rate of lichee polysaccharide can be up to 24.33%, and purity has improved 10%;
(2) the present invention adopts ultrasonic-microwave cooperating technology to extract lichee polysaccharide, and technology is simple, takes weak point, has kept lichee polysaccharide effective constituent simultaneously;
(3) the present invention has verified the immunoloregulation function of lichee polysaccharide, for new approach has been opened up in the deep processing of lichee.
Embodiment
Following examples only are used to set forth the present invention, do not limit protection scope of the present invention.Those skilled in the art all can realize purpose of the present invention according to above scope of disclosure.
(1) lichee polysaccharide of the present invention and extracting method thereof
Embodiment 1
Get fresh lichee, oven dry removes peeling and nuclear, gets Fructus Litchi, get Fructus Litchi 500g, adding 4kg volumetric concentration is 70% ethanolic soln backflow 6h, and the gained Fructus Litchi is pulverized after 70 ℃ of oven dry, gets and pulverizes back powder 300g, add 3kg water, regulating pH is 7.5, and ultrasonic-microwave cooperating extracts 5min, and setting ultrasonic power respectively is 150w, microwave power ratio is 200w, get extracting solution, with the extracting solution filtered through gauze, collect filtrate, the centrifugal 10min of 4000rpm, collect supernatant liquor, vacuum concentration is to 1/10 of original volume, adds volumetric concentration and is 85% ethanolic soln in concentrated solution and carry out alcohol precipitation, and the volumetric concentration that is added into ethanolic soln is 70%, take off layer precipitation, the centrifugal 10min of 4000rpm, collecting precipitation, vacuum lyophilization promptly gets lichee polysaccharide.
Embodiment 2
Get fresh lichee, oven dry removes peeling and nuclear, gets Fructus Litchi, get Fructus Litchi 600g, adding 6kg volumetric concentration is 75% ethanolic soln backflow 8h, and the gained Fructus Litchi is pulverized after 75 ℃ of oven dry, gets and pulverizes back powder 400g, add 8kg water, regulating pH is 8.5, and ultrasonic-microwave cooperating extracts 10min, and setting ultrasonic power respectively is 300w, microwave power ratio is 330w, get extracting solution, with the extracting solution filtered through gauze, collect filtrate, the centrifugal 15min of 5000rpm, collect supernatant liquor, vacuum concentration is to 1/4 of original volume, adds volumetric concentration and is 90% ethanolic soln in concentrated solution and carry out alcohol precipitation, and the volumetric concentration that is added into ethanolic soln is 75%, take off layer precipitation, the centrifugal 15min of 5000rpm, collecting precipitation, vacuum lyophilization promptly gets lichee polysaccharide.
Embodiment 3
Get fresh lichee, oven dry removes peeling and nuclear, gets Fructus Litchi, get Fructus Litchi 800g, adding 9.6kg volumetric concentration is 80% ethanolic soln backflow 8h, and the gained Fructus Litchi is pulverized after 80 ℃ of oven dry, gets and pulverizes back powder 500g, add 15kg water, regulating pH is 9.5, and ultrasonic-microwave cooperating extracts 15min, and setting ultrasonic power respectively is 500w, microwave power ratio is 500w, get extracting solution, with the extracting solution filtered through gauze, collect filtrate, the centrifugal 120min of 6000rpm, collect supernatant liquor, vacuum concentration is to 2/5 of original volume, adds volumetric concentration and is 95% ethanolic soln in concentrated solution and carry out alcohol precipitation, and the volumetric concentration that is added into ethanolic soln is 80%, take off layer precipitation, the centrifugal 20min of 6000rpm, collecting precipitation, vacuum lyophilization promptly gets lichee polysaccharide.
Embodiment 4
Lichee polysaccharide of the present invention precipitation is directly drunk, every day 5～10g, to human body have antiviral, strengthening immunity, antitumor, delay senility, effect such as hypoglycemic, anticoagulation.
Embodiment 5
Lichee polysaccharide of the present invention and secondary calcium phosphate by 1: 0.1 mixed, are prepared into capsule, every day 5～10g, to human body have antiviral, strengthening immunity, antitumor, delay senility, effect such as hypoglycemic, anticoagulation.
Embodiment 6
Lichee polysaccharide of the present invention and Zulkovsky starch, sweetener are prepared into medicinal granules by 1: 4: 0.04 mixed, every day 20～40g, to human body have antiviral, strengthening immunity, antitumor, delay senility, effect such as hypoglycemic, anticoagulation.
(2) test of pesticide effectiveness of lichee polysaccharide of the present invention:
1, test conditions
1.1 sample
The lichee polysaccharide powder of the inventive method preparation.
1.2 laboratory animal
The KM mouse, male, body weight 18-22g, the SPF level is provided by Nanfang Medical Univ's Experimental Animal Center.
1.3 grouping and processing
(1) grouping
Set up mouse separately 4 groups at random by body weight: the blank group; Model control group is basic, normal, high dosage group, and laboratory animal is divided into 3 batches, and 60 every batch, totally 4 groups, 15 every group, every batch of experiment periods is 30 days.
(2) handle
Blank group: irritate stomach equivalent distilled water;
Model group: 2 batches are respectively applied for that the mononuclear macrophage activate the phagocytic capacity is measured, serum hemolysin IgG assay, basic, normal, high dosage group every day respectively with 100,200, the 400mg/kgbw lichee polysaccharide irritates stomach, the blank group is irritated stomach equivalent distilled water;
Also have 1 batch to be used for that splenic lymphocyte transformation experiment, NK cytoactive are measured, mouse immune organ assessment of indices, basic, normal, high dosage group every day respectively with 50,100, the 200mg/kgbw lichee polysaccharide irritates stomach, the blank group is irritated stomach equivalent distilled water.
1.4 data analysis
Adopt SPSS to carry out data analysis.
2, test-results
Lichee polysaccharide to NK cytoactive, ConA induce splenic lymphocyte to transform, mouse thymus refers to and mice serum hemolysin HC
50Influence as shown in the table:
The effect that table 1 lichee polysaccharide is regulated mouse immune
Annotate: b and a compare, p＜0.05
Test-results shows:
(1) lichee polysaccharide 50mg/ (kgd) dosage group is compared with the blank group and can be significantly improved the NK cells in mice activity, 100mg/ (kgd) and 200mg/ (kgd) the dosage group then there was no significant difference of comparing with control group.
(2) lichee polysaccharide 50mg/ (kgd) dosage group is compared with control group and can be significantly improved ConA inductive mouse spleen lymphocyte conversion capability, 100mg/ (kgd) and 200mg/ (kgd) the dosage group then there was no significant difference of comparing with control group.
(3) lichee polysaccharide 100mg/ (kgd) and 200mg/ (kgd) the dosage group serum hemolysin HC that compares with control group and can make mouse
50There is significance to improve, and 400mg/ (kgd) the dosage group there was no significant difference of comparing with control group.
(4) lichee polysaccharide 100mg/ (kgd) dosage group is compared with control group the mouse thymus index is significantly increased, all the other the two groups then there was no significant differences of comparing with control group.
To sum up, lichee polysaccharide 50mg/ (kgd) dosage group is compared with control group and can be significantly improved the mouse spleen lymphocyte conversion capability that NK cells in mice is active and ConA induces; Compare with the control group serum hemolysin HC of mouse of lichee polysaccharide 100mg/ (kgd) and 200mg/ (kgd) dosage group50There is conspicuousness to improve; Lichee polysaccharide 100mg/ (kgd) dosage group is compared with control group the mouse thymus index is significantly increased, and this result illustrates that lichee polysaccharide has certain immunity enhancing effect to normal mouse.
Claims (4)
1. the extracting method of a lichee polysaccharide, it is characterized in that: the Fructus Litchi powder of selecting pulverizing for use, the water that adds its 10～30 times of weight, regulating pH is 7.5～9.5, ultrasonic-microwave cooperating extracts 5～15min, get extracting solution, through concentrating, precipitate the dry lichee polysaccharide that gets, described Fructus Litchi powdered preparation step: (1) selects the fresh lichee raw material, oven dry again, remove peeling and nuclear, get Fructus Litchi; (2) get Fructus Litchi, the ethanolic soln that adds its 8～12 times of weight refluxed 6～10 hours; (3) get Fructus Litchi after step (2) is handled, 70～85 ℃ of oven dry are pulverized; When ultrasonic-microwave cooperating extracts, volume containing the sample and ultrasonic power ratio are 1.0～2.0g/w, volume containing the sample and microwave power ratio are 1.0～1.5g/w, the precipitation drying process is: add ethanol and carry out alcohol precipitation in concentrated solution, take off layer precipitation, the centrifugal 10～20min of 4000～6000rpm, collecting precipitation, vacuum-drying promptly gets lichee polysaccharide, and precipitation drying process used alcoholic acid volume percent is 85～95%, is added into that the alcoholic acid volume percent is 70～80% in the concentrated solution.
2. according to the extracting method of the described lichee polysaccharide of claim 1, it is characterized in that: in Fructus Litchi powdered preparation step, the volume percent of the ethanolic soln described in the step (2) is 70～85%.
3. according to the extracting method of the described lichee polysaccharide of claim 1, it is characterized in that: the extracting solution concentration process: with extracting liquid filtering, collect filtrate, the centrifugal 10～20min of 4000～6000rpm collects supernatant liquor, concentrates, and gets concentrated solution.
4. according to the extracting method of the described lichee polysaccharide of claim 3, it is characterized in that: collect supernatant concentration to 1/10～2/5 of original volume.
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| CN101434661B true CN101434661B (en) | 2010-10-13 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101979639A (en) * | 2010-09-27 | 2011-02-23 | 广东光宇生物科技有限公司 | Dendrobium polysaccharide with hypoglycemic activity and extracting method thereof |
| CN102630890B (en) * | 2012-04-25 | 2014-11-19 | 集美大学 | Method for preparing leechee or longan pulp powder |
| CN104292354A (en) * | 2014-11-11 | 2015-01-21 | 济南凯因生物科技有限公司 | Extraction method of litchi polysaccharide |
| CN104522767B (en) * | 2014-12-10 | 2017-11-24 | 惠州学院 | A kind of lichee active polysaccharide probiotics drink and its production method |
| CN104725519A (en) * | 2015-03-09 | 2015-06-24 | 梁永锋 | Method for extracting and purifying banana peel polysaccharide |
| CN104945529B (en) * | 2015-07-10 | 2017-06-16 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of active spike lower intestinal tract immunoregulatory activity lichee polysaccharide fraction preparation method |
| CN114617779A (en) * | 2022-04-11 | 2022-06-14 | 深圳地标农品农业科技有限公司 | Litchi mask and preparation method thereof |
| CN115500431A (en) * | 2022-09-02 | 2022-12-23 | 深圳市质量安全检验检测研究院 | Pet feed capable of improving immunity and preparation method thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1979133A (en) * | 2005-11-30 | 2007-06-13 | 广东省农业科学院农业生物技术研究所 | Method for detecting leechee ploysaccharose content |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1979133A (en) * | 2005-11-30 | 2007-06-13 | 广东省农业科学院农业生物技术研究所 | Method for detecting leechee ploysaccharose content |
Non-Patent Citations (1)
| Title |
|---|
| 唐小俊等.微波提取荔枝多糖及冲剂的研制.《食品研究与开发》.2008,第29卷(第1期),18-21. * |
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