CN101400786A - 细胞的富集 - Google Patents

细胞的富集 Download PDF

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Publication number
CN101400786A
CN101400786A CNA2007800087785A CN200780008778A CN101400786A CN 101400786 A CN101400786 A CN 101400786A CN A2007800087785 A CNA2007800087785 A CN A2007800087785A CN 200780008778 A CN200780008778 A CN 200780008778A CN 101400786 A CN101400786 A CN 101400786A
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China
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cell
msc
mscs
collagenase
lngfr
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CNA2007800087785A
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Chinese (zh)
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丹尼斯·麦戈纳格尔
埃莱娜·约内斯
安妮·英格利希
保罗·艾莫里
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University of Leeds
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University of Leeds
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/04—Drugs for skeletal disorders for non-specific disorders of the connective tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Cardiology (AREA)
  • Hematology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Hospice & Palliative Care (AREA)
  • Transplantation (AREA)
  • Psychology (AREA)
  • Pain & Pain Management (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CNA2007800087785A 2006-01-18 2007-01-17 细胞的富集 Pending CN101400786A (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0600972.4A GB0600972D0 (en) 2006-01-18 2006-01-18 Enrichment of cells
GB0600972.4 2006-01-18

Publications (1)

Publication Number Publication Date
CN101400786A true CN101400786A (zh) 2009-04-01

Family

ID=36010490

Family Applications (1)

Application Number Title Priority Date Filing Date
CNA2007800087785A Pending CN101400786A (zh) 2006-01-18 2007-01-17 细胞的富集

Country Status (7)

Country Link
US (1) US20100260721A1 (https=)
EP (1) EP1979471A1 (https=)
JP (1) JP2009523438A (https=)
CN (1) CN101400786A (https=)
AU (1) AU2007206750A1 (https=)
GB (1) GB0600972D0 (https=)
WO (1) WO2007083093A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102971414A (zh) * 2009-12-22 2013-03-13 新加坡科技研究局 骨折的治疗
CN107796936A (zh) * 2016-08-31 2018-03-13 宣捷生物科技股份有限公司 区别间充质干细胞的方法及测定间充质干细胞的纯度的方法
CN108635377A (zh) * 2011-09-09 2018-10-12 麦瑟布莱斯特公司 增加成骨细胞功能的方法

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2010003019A (es) 2007-09-19 2010-04-30 Pluristem Ltd Celulas adherentes de los tejidos adiposo y de placenta y uso de las mismas en la terapia.
JP5645197B2 (ja) * 2009-06-23 2014-12-24 学校法人日本大学 幹細胞の未分化状態を維持する新規方法
JP2013507931A (ja) 2009-10-16 2013-03-07 ユニバーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー 再生医療用途のための接着性骨髄幹細胞の閉鎖系統分離システム
US20140314869A1 (en) * 2011-08-29 2014-10-23 Arnold I. Caplan Isolating and therapeutic use of perivascular medicinal cells
CN103266081B (zh) * 2012-01-21 2015-05-13 中国人民解放军军事医学科学院附属医院 从脐带中分离培养间充质干细胞的高效方法
JP6452695B2 (ja) * 2013-08-06 2019-01-16 リジェネックス， エルエルシー 骨髄脂肪部分単離のデバイスおよび方法
EP2998392A1 (en) * 2014-09-16 2016-03-23 The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin A population of cells for use in single-stage cell-based cartilage regeneration
TWI566774B (zh) 2014-10-06 2017-01-21 佛教慈濟醫療財團法人花蓮慈濟醫院 治療關節疾病的組成物及其方法
WO2016111899A1 (en) 2015-01-05 2016-07-14 Petrucci Gary M Methods and materials for treating lung disorders
WO2016111900A1 (en) * 2015-01-05 2016-07-14 Petrucci Gary M Methods and materials for treating arthritis
US10993969B2 (en) 2016-02-05 2021-05-04 Gary M. Petrucci Methods and materials for treating nerve injuries and neurological disorders
WO2018207918A1 (ja) * 2017-05-12 2018-11-15 国立大学法人 東京医科歯科大学 移植効率を向上させる間葉系幹細胞の純化方法
US10478531B2 (en) 2017-06-22 2019-11-19 Gary M. Petrucci Methods and materials for treating blood vessels
US10251917B1 (en) 2017-09-19 2019-04-09 Gary M. Petrucci Methods and materials for treating tumors
US11285177B2 (en) 2018-01-03 2022-03-29 Globus Medical, Inc. Allografts containing viable cells and methods thereof
WO2020161748A1 (en) * 2019-02-08 2020-08-13 Regrow Biosciences Private Limited Method for mesenchymal stem cell isolation and osteoblast differentiation
US11697799B2 (en) 2019-04-15 2023-07-11 Ossium Health, Inc. System and method for extraction and cryopreservation of bone marrow
EP4181675A4 (en) 2020-07-18 2024-04-24 Ossium Health, Inc. PERMEATION OF WHOLE VERTEBRATE BODIES WITH A CRYOPRETECTIVE AGENT USING VACUUM-ASSISTED DIFFUSION
CA3195653A1 (en) 2020-10-14 2022-04-21 Ossium Health, Inc. Systems and methods for extraction and cryopreservation of bone marrow
WO2022133282A1 (en) 2020-12-18 2022-06-23 Ossium Health, Inc. Methods of cell therapies
KR102858598B1 (ko) * 2025-01-16 2025-09-10 신종인 콜라게나제를 이용한 지방세포 이식용 조성물의 제조방법

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5226914A (en) * 1990-11-16 1993-07-13 Caplan Arnold I Method for treating connective tissue disorders
US5811094A (en) * 1990-11-16 1998-09-22 Osiris Therapeutics, Inc. Connective tissue regeneration using human mesenchymal stem cell preparations
US5486359A (en) * 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US6653134B2 (en) * 1995-03-28 2003-11-25 Cp Hahnemann University Isolated stromal cells for use in the treatment of diseases of the central nervous system
US6482231B1 (en) * 1995-11-20 2002-11-19 Giovanni Abatangelo Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative
ATE439849T1 (de) * 1996-04-19 2009-09-15 Osiris Therapeutics Inc Die wiederherstellung und verstärkung von knochen mittels mesenchymalen stammzellen
US6835377B2 (en) * 1998-05-13 2004-12-28 Osiris Therapeutics, Inc. Osteoarthritis cartilage regeneration
US6893900B1 (en) * 1998-06-24 2005-05-17 Amkor Technology, Inc. Method of making an integrated circuit package
US7670628B2 (en) * 1999-07-07 2010-03-02 Angioblast Systems, Inc. Mesenchymal precursor cell
AU5723601A (en) * 2000-04-25 2001-11-07 Osiris Therapeutics Inc Joint repair using mesenchymal stem cells
DE60120151D1 (de) * 2000-04-28 2006-07-06 Childrens Medical Center Isolierung von mesenchymalen stammzellen und deren verwendung
US20030103947A1 (en) * 2001-02-23 2003-06-05 Urlich Noth In vitro engineered cartilage constructs produced by coating biodegradable polymer with human mesenchymal stem cells
US20040033217A1 (en) * 2002-05-31 2004-02-19 Padmavathy Vanguri Intraperitoneal delivery of genetically engineered mesenchymal stem cells
WO2005001080A2 (en) * 2003-06-27 2005-01-06 Ethicon, Incorporated Postpartum-derived cells for use in treatment of disease of the heart and circulatory system

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102971414A (zh) * 2009-12-22 2013-03-13 新加坡科技研究局 骨折的治疗
CN102971414B (zh) * 2009-12-22 2016-01-27 新加坡科技研究局 骨折的治疗
CN108635377A (zh) * 2011-09-09 2018-10-12 麦瑟布莱斯特公司 增加成骨细胞功能的方法
CN108635377B (zh) * 2011-09-09 2022-06-24 麦瑟布莱斯特公司 增加成骨细胞功能的方法
CN107796936A (zh) * 2016-08-31 2018-03-13 宣捷生物科技股份有限公司 区别间充质干细胞的方法及测定间充质干细胞的纯度的方法

Also Published As

Publication number Publication date
EP1979471A1 (en) 2008-10-15
GB0600972D0 (en) 2006-03-01
AU2007206750A1 (en) 2007-07-26
JP2009523438A (ja) 2009-06-25
WO2007083093A1 (en) 2007-07-26
US20100260721A1 (en) 2010-10-14

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