JP2009523438A - 細胞の富化 - Google Patents
細胞の富化 Download PDFInfo
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- JP2009523438A JP2009523438A JP2008550832A JP2008550832A JP2009523438A JP 2009523438 A JP2009523438 A JP 2009523438A JP 2008550832 A JP2008550832 A JP 2008550832A JP 2008550832 A JP2008550832 A JP 2008550832A JP 2009523438 A JP2009523438 A JP 2009523438A
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| PCT/GB2007/000107 WO2007083093A1 (en) | 2006-01-18 | 2007-01-17 | Enrichment of cells |
Publications (2)
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| JP2009523438A true JP2009523438A (ja) | 2009-06-25 |
| JP2009523438A5 JP2009523438A5 (https=) | 2010-03-04 |
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| US (1) | US20100260721A1 (https=) |
| EP (1) | EP1979471A1 (https=) |
| JP (1) | JP2009523438A (https=) |
| CN (1) | CN101400786A (https=) |
| AU (1) | AU2007206750A1 (https=) |
| GB (1) | GB0600972D0 (https=) |
| WO (1) | WO2007083093A1 (https=) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016074648A (ja) * | 2014-10-06 | 2016-05-12 | 佛教慈濟醫療財團法人 | 関節疾患を治療するための組成物及び方法 |
| KR102858598B1 (ko) * | 2025-01-16 | 2025-09-10 | 신종인 | 콜라게나제를 이용한 지방세포 이식용 조성물의 제조방법 |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2010003019A (es) | 2007-09-19 | 2010-04-30 | Pluristem Ltd | Celulas adherentes de los tejidos adiposo y de placenta y uso de las mismas en la terapia. |
| JP5645197B2 (ja) * | 2009-06-23 | 2014-12-24 | 学校法人日本大学 | 幹細胞の未分化状態を維持する新規方法 |
| JP2013507931A (ja) | 2009-10-16 | 2013-03-07 | ユニバーシティ オブ メディスン アンド デンティストリー オブ ニュー ジャージー | 再生医療用途のための接着性骨髄幹細胞の閉鎖系統分離システム |
| AU2010335005B2 (en) * | 2009-12-22 | 2016-04-21 | Agency For Science, Technology And Research | Treatment of bone fracture |
| US20140314869A1 (en) * | 2011-08-29 | 2014-10-23 | Arnold I. Caplan | Isolating and therapeutic use of perivascular medicinal cells |
| US9642878B2 (en) * | 2011-09-09 | 2017-05-09 | Mesoblast, Inc. | Methods for increasing osteoblastic function |
| CN103266081B (zh) * | 2012-01-21 | 2015-05-13 | 中国人民解放军军事医学科学院附属医院 | 从脐带中分离培养间充质干细胞的高效方法 |
| JP6452695B2 (ja) * | 2013-08-06 | 2019-01-16 | リジェネックス， エルエルシー | 骨髄脂肪部分単離のデバイスおよび方法 |
| EP2998392A1 (en) * | 2014-09-16 | 2016-03-23 | The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin | A population of cells for use in single-stage cell-based cartilage regeneration |
| WO2016111899A1 (en) | 2015-01-05 | 2016-07-14 | Petrucci Gary M | Methods and materials for treating lung disorders |
| WO2016111900A1 (en) * | 2015-01-05 | 2016-07-14 | Petrucci Gary M | Methods and materials for treating arthritis |
| US10993969B2 (en) | 2016-02-05 | 2021-05-04 | Gary M. Petrucci | Methods and materials for treating nerve injuries and neurological disorders |
| US20180059109A1 (en) * | 2016-08-31 | 2018-03-01 | Meridigen Biotech Co., Ltd. | Method of distinguishing mesenchymal stem cells and method of determining purity of mesenchymal stem cells |
| WO2018207918A1 (ja) * | 2017-05-12 | 2018-11-15 | 国立大学法人 東京医科歯科大学 | 移植効率を向上させる間葉系幹細胞の純化方法 |
| US10478531B2 (en) | 2017-06-22 | 2019-11-19 | Gary M. Petrucci | Methods and materials for treating blood vessels |
| US10251917B1 (en) | 2017-09-19 | 2019-04-09 | Gary M. Petrucci | Methods and materials for treating tumors |
| US11285177B2 (en) | 2018-01-03 | 2022-03-29 | Globus Medical, Inc. | Allografts containing viable cells and methods thereof |
| WO2020161748A1 (en) * | 2019-02-08 | 2020-08-13 | Regrow Biosciences Private Limited | Method for mesenchymal stem cell isolation and osteoblast differentiation |
| US11697799B2 (en) | 2019-04-15 | 2023-07-11 | Ossium Health, Inc. | System and method for extraction and cryopreservation of bone marrow |
| EP4181675A4 (en) | 2020-07-18 | 2024-04-24 | Ossium Health, Inc. | PERMEATION OF WHOLE VERTEBRATE BODIES WITH A CRYOPRETECTIVE AGENT USING VACUUM-ASSISTED DIFFUSION |
| CA3195653A1 (en) | 2020-10-14 | 2022-04-21 | Ossium Health, Inc. | Systems and methods for extraction and cryopreservation of bone marrow |
| WO2022133282A1 (en) | 2020-12-18 | 2022-06-23 | Ossium Health, Inc. | Methods of cell therapies |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002504503A (ja) * | 1998-02-24 | 2002-02-12 | エムシーピー・ハーネマン・ユニバーシテイ | 中枢神経系の疾病の処置における使用のための単離されたストロマ細胞 |
| US20030103947A1 (en) * | 2001-02-23 | 2003-06-05 | Urlich Noth | In vitro engineered cartilage constructs produced by coating biodegradable polymer with human mesenchymal stem cells |
| US20040033217A1 (en) * | 2002-05-31 | 2004-02-19 | Padmavathy Vanguri | Intraperitoneal delivery of genetically engineered mesenchymal stem cells |
| WO2005001078A2 (en) * | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Regeneration and repair of neural tissue using postpartum-derived cells |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5226914A (en) * | 1990-11-16 | 1993-07-13 | Caplan Arnold I | Method for treating connective tissue disorders |
| US5811094A (en) * | 1990-11-16 | 1998-09-22 | Osiris Therapeutics, Inc. | Connective tissue regeneration using human mesenchymal stem cell preparations |
| US5486359A (en) * | 1990-11-16 | 1996-01-23 | Osiris Therapeutics, Inc. | Human mesenchymal stem cells |
| US6482231B1 (en) * | 1995-11-20 | 2002-11-19 | Giovanni Abatangelo | Biological material for the repair of connective tissue defects comprising mesenchymal stem cells and hyaluronic acid derivative |
| ATE439849T1 (de) * | 1996-04-19 | 2009-09-15 | Osiris Therapeutics Inc | Die wiederherstellung und verstärkung von knochen mittels mesenchymalen stammzellen |
| US6835377B2 (en) * | 1998-05-13 | 2004-12-28 | Osiris Therapeutics, Inc. | Osteoarthritis cartilage regeneration |
| US6893900B1 (en) * | 1998-06-24 | 2005-05-17 | Amkor Technology, Inc. | Method of making an integrated circuit package |
| US7670628B2 (en) * | 1999-07-07 | 2010-03-02 | Angioblast Systems, Inc. | Mesenchymal precursor cell |
| AU5723601A (en) * | 2000-04-25 | 2001-11-07 | Osiris Therapeutics Inc | Joint repair using mesenchymal stem cells |
| DE60120151D1 (de) * | 2000-04-28 | 2006-07-06 | Childrens Medical Center | Isolierung von mesenchymalen stammzellen und deren verwendung |
-
2006
- 2006-01-18 GB GBGB0600972.4A patent/GB0600972D0/en not_active Ceased
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2007
- 2007-01-17 AU AU2007206750A patent/AU2007206750A1/en not_active Abandoned
- 2007-01-17 EP EP07700381A patent/EP1979471A1/en not_active Withdrawn
- 2007-01-17 CN CNA2007800087785A patent/CN101400786A/zh active Pending
- 2007-01-17 WO PCT/GB2007/000107 patent/WO2007083093A1/en not_active Ceased
- 2007-01-17 JP JP2008550832A patent/JP2009523438A/ja active Pending
- 2007-01-17 US US12/223,056 patent/US20100260721A1/en not_active Abandoned
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002504503A (ja) * | 1998-02-24 | 2002-02-12 | エムシーピー・ハーネマン・ユニバーシテイ | 中枢神経系の疾病の処置における使用のための単離されたストロマ細胞 |
| US20030103947A1 (en) * | 2001-02-23 | 2003-06-05 | Urlich Noth | In vitro engineered cartilage constructs produced by coating biodegradable polymer with human mesenchymal stem cells |
| US20040033217A1 (en) * | 2002-05-31 | 2004-02-19 | Padmavathy Vanguri | Intraperitoneal delivery of genetically engineered mesenchymal stem cells |
| WO2005001078A2 (en) * | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Regeneration and repair of neural tissue using postpartum-derived cells |
| WO2005001079A2 (en) * | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Soft tissue repair and regeneration using postpartum-derived cells |
| WO2005001076A2 (en) * | 2003-06-27 | 2005-01-06 | Ethicon, Incorporated | Postpartum cells derived from placental tissue, and methods of making and using the same |
| WO2005003334A2 (en) * | 2003-06-27 | 2005-01-13 | Ethicon, Incorporated | Postpartum cells derived from umbilical cord tissue, and methods of making and using the same |
| WO2005038012A2 (en) * | 2003-06-27 | 2005-04-28 | Ethicon Incorporated | Cartilage and bone repair and regeneration using postpartum-derived cells |
Non-Patent Citations (3)
| Title |
|---|
| JPN6012038749; Ｍｏｌ Ｂｉｏｔｅｃｈｎｏｌ． ２００３ Ｊａｎ；２３（１）：３７-４９． * |
| JPN6012038750; Ｔｒａｎｓｐｌａｎｔ Ｐｒｏｃ． ２００５ Ｎｏｖ；３７（９）：４０８０-３． * |
| JPN6012038751; Ａｒｔｈｒｉｔｉｓ Ｒｈｅｕｍ． ２００２ Ｄｅｃ；４６（１２）：３３４９-６０． * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2016074648A (ja) * | 2014-10-06 | 2016-05-12 | 佛教慈濟醫療財團法人 | 関節疾患を治療するための組成物及び方法 |
| US10624928B2 (en) | 2014-10-06 | 2020-04-21 | Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation | Composition for treatment of joint disease and method thereof |
| KR102858598B1 (ko) * | 2025-01-16 | 2025-09-10 | 신종인 | 콜라게나제를 이용한 지방세포 이식용 조성물의 제조방법 |
Also Published As
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| EP1979471A1 (en) | 2008-10-15 |
| GB0600972D0 (en) | 2006-03-01 |
| AU2007206750A1 (en) | 2007-07-26 |
| WO2007083093A1 (en) | 2007-07-26 |
| CN101400786A (zh) | 2009-04-01 |
| US20100260721A1 (en) | 2010-10-14 |
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