CN101348518A - 一种使用羧甲基离子交换色谱纯化贻贝粘蛋白的方法 - Google Patents
一种使用羧甲基离子交换色谱纯化贻贝粘蛋白的方法 Download PDFInfo
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- CN101348518A CN101348518A CNA2007101794925A CN200710179492A CN101348518A CN 101348518 A CN101348518 A CN 101348518A CN A2007101794925 A CNA2007101794925 A CN A2007101794925A CN 200710179492 A CN200710179492 A CN 200710179492A CN 101348518 A CN101348518 A CN 101348518A
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- mussel mucin
- sea
- mussel
- sodium
- ion exchange
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- 238000000034 method Methods 0.000 title claims abstract description 14
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 title claims abstract description 12
- 238000004255 ion exchange chromatography Methods 0.000 title abstract description 5
- 241000237536 Mytilus edulis Species 0.000 claims abstract description 19
- 238000005342 ion exchange Methods 0.000 claims abstract description 15
- 235000020638 mussel Nutrition 0.000 claims abstract description 15
- 238000000746 purification Methods 0.000 claims abstract description 12
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 claims abstract description 9
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 238000001914 filtration Methods 0.000 claims abstract description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 24
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 16
- 102000004169 proteins and genes Human genes 0.000 claims description 15
- 108090000623 proteins and genes Proteins 0.000 claims description 15
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- 238000000108 ultra-filtration Methods 0.000 claims description 12
- 229920002684 Sepharose Polymers 0.000 claims description 11
- WFAJBYGUONQMCI-UHFFFAOYSA-N acetic acid urea Chemical compound NC(=O)N.NC(=O)N.NC(=O)N.NC(=O)N.NC(=O)N.NC(=O)N.C(C)(=O)O WFAJBYGUONQMCI-UHFFFAOYSA-N 0.000 claims description 8
- 238000004043 dyeing Methods 0.000 claims description 8
- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 claims description 8
- DAJSVUQLFFJUSX-UHFFFAOYSA-M sodium;dodecane-1-sulfonate Chemical compound [Na+].CCCCCCCCCCCCS([O-])(=O)=O DAJSVUQLFFJUSX-UHFFFAOYSA-M 0.000 claims description 8
- 238000010186 staining Methods 0.000 claims description 8
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- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 5
- 229920005654 Sephadex Polymers 0.000 claims description 4
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- 238000004321 preservation Methods 0.000 claims description 4
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- WTDRDQBEARUVNC-LURJTMIESA-N L-DOPA Chemical group OC(=O)[C@@H](N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-LURJTMIESA-N 0.000 abstract description 7
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- WTDRDQBEARUVNC-UHFFFAOYSA-N L-Dopa Natural products OC(=O)C(N)CC1=CC=C(O)C(O)=C1 WTDRDQBEARUVNC-UHFFFAOYSA-N 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
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- JPXMTWWFLBLUCD-UHFFFAOYSA-N nitro blue tetrazolium(2+) Chemical compound COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 JPXMTWWFLBLUCD-UHFFFAOYSA-N 0.000 abstract 1
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- 102000015728 Mucins Human genes 0.000 description 35
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- 235000000177 Indigofera tinctoria Nutrition 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 239000004793 Polystyrene Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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- 230000005847 immunogenicity Effects 0.000 description 1
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- 230000007246 mechanism Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 229940051875 mucins Drugs 0.000 description 1
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- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
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- 125000001493 tyrosinyl group Chemical group [H]OC1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
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CN2007101794925A CN101348518B (zh) | 2007-12-14 | 2007-12-14 | 一种使用羧甲基离子交换色谱纯化贻贝粘蛋白的方法 |
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CN2007101794925A CN101348518B (zh) | 2007-12-14 | 2007-12-14 | 一种使用羧甲基离子交换色谱纯化贻贝粘蛋白的方法 |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013143077A1 (zh) * | 2012-03-28 | 2013-10-03 | 江阴贝瑞森生化技术有限公司 | 使用多模式色谱纯化贻贝粘蛋白的方法 |
WO2014186937A1 (zh) * | 2013-05-20 | 2014-11-27 | Gao Min | 贻贝粘蛋白凝胶的制备方法、贻贝粘蛋白凝胶及其应用 |
CN108676089A (zh) * | 2018-05-23 | 2018-10-19 | 遵义医学院 | 一种高活性卵类粘蛋白的分离方法 |
CN109265528A (zh) * | 2018-09-19 | 2019-01-25 | 南方医科大学 | 一种贻贝粘蛋白的制备方法 |
US10485848B2 (en) | 2015-08-14 | 2019-11-26 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and use thereof for treating mucosal inflammation |
US10568938B2 (en) | 2015-07-20 | 2020-02-25 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and applications thereof in suppression of skin inflammations |
US10675327B2 (en) | 2015-07-20 | 2020-06-09 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Applications of mussel adhesive protein product in treatment and prevention of diseases related to melanin |
US11260111B2 (en) | 2015-07-20 | 2022-03-01 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and applications thereof in suppression of skin inflammations |
CN114456250A (zh) * | 2022-03-30 | 2022-05-10 | 成都英普博集生物科技有限公司 | 一种类贻贝粘蛋白的纯化工艺 |
CN114853863A (zh) * | 2022-04-28 | 2022-08-05 | 西安德诺海思医疗科技有限公司 | 一种重组类贻贝粘蛋白纯化方法 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1415628A (zh) * | 2002-08-14 | 2003-05-07 | 上海天伟生物制药有限公司 | 一种提取人尿黄体生成素的方法 |
US6987170B1 (en) * | 2004-08-09 | 2006-01-17 | Battelle Energy Alliance, Llc | Cloning and expression of recombinant adhesive protein Mefp-1 of the blue mussel, Mytilus edulis |
-
2007
- 2007-12-14 CN CN2007101794925A patent/CN101348518B/zh active Active
Cited By (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013143077A1 (zh) * | 2012-03-28 | 2013-10-03 | 江阴贝瑞森生化技术有限公司 | 使用多模式色谱纯化贻贝粘蛋白的方法 |
WO2014186937A1 (zh) * | 2013-05-20 | 2014-11-27 | Gao Min | 贻贝粘蛋白凝胶的制备方法、贻贝粘蛋白凝胶及其应用 |
US10568938B2 (en) | 2015-07-20 | 2020-02-25 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and applications thereof in suppression of skin inflammations |
US10675327B2 (en) | 2015-07-20 | 2020-06-09 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Applications of mussel adhesive protein product in treatment and prevention of diseases related to melanin |
US11090360B2 (en) | 2015-07-20 | 2021-08-17 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Applications of mussel adhesive protein product in treatment and prevention of diseases related to melanin |
US11260111B2 (en) | 2015-07-20 | 2022-03-01 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and applications thereof in suppression of skin inflammations |
US11458190B2 (en) | 2015-08-14 | 2022-10-04 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and use thereof for treating mucosal inflammation |
US10485848B2 (en) | 2015-08-14 | 2019-11-26 | Jiangyin Bengt I. Samuelsson Institute Of Life Science Co., Ltd. | Mussel adhesive protein product and use thereof for treating mucosal inflammation |
CN108676089A (zh) * | 2018-05-23 | 2018-10-19 | 遵义医学院 | 一种高活性卵类粘蛋白的分离方法 |
CN109265528A (zh) * | 2018-09-19 | 2019-01-25 | 南方医科大学 | 一种贻贝粘蛋白的制备方法 |
CN114456250A (zh) * | 2022-03-30 | 2022-05-10 | 成都英普博集生物科技有限公司 | 一种类贻贝粘蛋白的纯化工艺 |
CN114456250B (zh) * | 2022-03-30 | 2023-12-26 | 成都英普博集生物科技有限公司 | 一种类贻贝粘蛋白的纯化工艺 |
CN114853863A (zh) * | 2022-04-28 | 2022-08-05 | 西安德诺海思医疗科技有限公司 | 一种重组类贻贝粘蛋白纯化方法 |
CN114853863B (zh) * | 2022-04-28 | 2023-11-21 | 西安德诺海思医疗科技有限公司 | 一种重组类贻贝粘蛋白纯化方法 |
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CN101348518B (zh) | 2011-09-28 |
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