CN101341856A - Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell - Google Patents
Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell Download PDFInfo
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- CN101341856A CN101341856A CNA2007100152860A CN200710015286A CN101341856A CN 101341856 A CN101341856 A CN 101341856A CN A2007100152860 A CNA2007100152860 A CN A2007100152860A CN 200710015286 A CN200710015286 A CN 200710015286A CN 101341856 A CN101341856 A CN 101341856A
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- ovum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to the aquiculture field, in particular to an eutelegenesis method for improving the ovum fertilization rate of spotted halibut. The method is an eutelegenesis technology for sea fish spotted halibut ripe fish, which solves the problems that because the ovulatory cycle of the spotted halibut which is artificially raised is unstable, the inside of ovarian cavity is usually overripe, and among the artificially extruded ovum, the overripe ovum is often mixed with normal ovum, the fertilization rate of eutelegenesis is very low, and batch fertilized eggs are difficult to be obtained, etc. In the eutelegenesis method for improving the ovum fertilization rate of spotted halibut, the spotted halibut ovum is fertilized in high-salt seawater after being separated in the high-salt seawater. The method improves the fertilization rate of the spotted halibut ovum, obtains batch fertilized eggs, lays technical foundation for adding a novel aquaculture breed for the marine aquaculture industry of our country, and has significant meaning in developing the industrialization of spotted halibut.
Description
Technical field
The invention belongs to aquaculture field, specifically the verasper moseri ovum is separated laggard pedestrian worker's insemination through high sea brine, to improve the method for fertilization rate.
Background technology
Verasper moseri mainly is distributed in to the east of Sea of Okhotsk, marine site, Japanese northwest, the Korea peninsula and southwestern sea area and Chinese Huanghai Sea and Bohai Sea, is the cold warm nature demersal fishes of Pacific Northwest.The verasper moseri bodily form is bigger, and growth rate is very fast, and spur is few, visceral mass is little, and edible part is many, is good breed variety.
Verasper moseri is the ovulatory cycle instability under artificial feeding, and is usually overdone in the ovarian cavity.The situation that overdone ovum mixes with normal ovum often appears in the ovum of manually extruding, and test-tube fertilization rate is very low, is difficult to obtain fertilized egg in batches; For above-mentioned reasons, seriously restricting the raising of verasper moseri artificial propagation techniques.
Summary of the invention
The object of the present invention is to provide a kind of artificial insemination method that improves germ cell fertility rate of verasper moseri germ cell, this method is the technology of artificial insemination of marine fishes veraspei moseri, solve because verasper moseri ovulatory cycle instability under artificial feeding, usually overdone in the ovarian cavity, the situation that overdone ovum mixes with normal ovum often appears in the ovum of manually extruding, test-tube fertilization rate is very low, is difficult to obtain problems such as batch fertilized egg.
For achieving the above object, the technical scheme taked of the present invention is:
The ovum that female veraspei moseri is manually extruded is put into and is equipped with that salinity is 38～42, temperature is the static separation of graduated cylinder of 7～8 ℃ of seawater, get the come-up ovum, the ovum filter is done, at once put into simultaneously with sperm that salinity is 34～36, temperature is that 7～8 ℃ seawater stirs evenly static 15～20 minutes; With salinity be 32, temperature is that 7～8 ℃ seawater changes water, to water clear till.Incubating oosperm keeps continuous charge (oxygenation, stirring) and flowing water (maintenance ocean temperature), and water temperature is that 8～10 ℃, seawater salinity are 32.
The present invention is that the verasper moseri ovum separates the test-tube process of laggard row through high sea brine, is meant the high sea brine of artificial preparation, overdone streak star ovum is separated with normal ovum, and then the process of in the high sea brine of artificial preparation, inseminating.The present invention has following advantage:
1, adopt the inventive method, overdone ovum can be separated with normal ovum, fertilization rate reached is more than 70%;
2, adopt the inventive method, in the production of verasper moseri scale seed, obtain the batch high quality fertilized egg of scophthatmus and established technical foundation.
3, the present invention has great significance for aspects such as the seed technological development of the famous and precious fish of this seawater of verasper moseri and sustainable breed.
Embodiment
Embodiment:
On November 7th, 2006 began the artificial culture of veraspei moseri at Golden Beach, Qingdao aquatic products Co., Ltd nursery, and breeding water is a nature seawater.Parent fish rearing is nature seawater in earlier stage, and water temperature is 5.8～15 ℃, and water temperature is reduced to 5.8 ℃ and kept 7 days, heats 0.3～0.5 ℃ to 7.5 ℃ then every day.6, on March of parent fish rearing to 2007, the parent population gonadal maturation.
The verasper moseri ovum that on March 17th, 2007,8:00 manually extruded 300ml is put into and is equipped with that salinity is 38, temperature is the static separation of 2000ml graduated cylinder of 7 ℃ of seawater, get the come-up ovum, the ovum filter is done, at once put into simultaneously with sperm that salinity is 34, temperature is that 7 ℃ 500ml seawater stirs evenly, smart ovum quantity is than being (2000～2500): 1, and static 15 minutes.With salinity is 32, and temperature is that 7 ℃ seawater changes water number time, to water clear till.Incubating oosperm keeps continuous charge (aeration quantity is 60L/ hour) and miniflow water (seawater flow is 5L/min), and water volume is 0.3m
3, water temperature is that 8～10 ℃, salinity are 32.Behind the incubating oosperm 5 days, it is 78.5% that the sampling counting is measured fertilization rate.
The verasper moseri ovum that on March 19th, 2007,8:00 manually extruded 300ml is put into and is equipped with that salinity is 42, temperature is the static separation of 2000ml graduated cylinder of 8 ℃ of seawater, get the come-up ovum, the ovum filter is done, at once put into simultaneously with sperm that salinity is 36, temperature is that 8 ℃ 500ml seawater stirs evenly, smart ovum quantity is than being (1000～1500): 1, and static 20 minutes.With salinity is 32, and temperature is that 8 ℃ seawater changes water number time, to water clear till.Incubating oosperm keeps continuous charge (aeration quantity is 60L/ hour) and miniflow water (seawater flow is 5L/min), and water volume is 0.3m
3, water temperature is that 8～10 ℃, salinity are 32.Behind the incubating oosperm 5 days, it is 80.2% that the sampling counting is measured fertilization rate.
Claims (3)
1, a kind of artificial insemination method that improves germ cell fertility rate of verasper moseri germ cell, it is characterized in that, the ovum that female veraspei moseri is manually extruded is put into and is equipped with that salinity is 38～42, temperature is the static separation of graduated cylinder of 7～8 ℃ of seawater, get the come-up ovum, the ovum filter is done, at once put into simultaneously with sperm that salinity is 34～36, temperature is that 7～8 ℃ seawater stirs evenly static 15～20 minutes; With salinity be 32, temperature is that 7～8 ℃ seawater changes water, to water clear till.
According to the artificial insemination method of the described raising germ cell fertility rate of verasper moseri germ cell of claim 1, it is characterized in that 2, incubating oosperm keeps continuous charge and flowing water, water temperature is that 8～10 ℃, seawater salinity are 32.
According to the artificial insemination method of the described raising germ cell fertility rate of verasper moseri germ cell of claim 1, it is characterized in that 3, smart ovum quantity is than being (1000～3000): 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100152860A CN101341856B (en) | 2007-07-11 | 2007-07-11 | Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007100152860A CN101341856B (en) | 2007-07-11 | 2007-07-11 | Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101341856A true CN101341856A (en) | 2009-01-14 |
| CN101341856B CN101341856B (en) | 2010-09-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2007100152860A Expired - Fee Related CN101341856B (en) | 2007-07-11 | 2007-07-11 | Artificial insemination method for improving germ cell fertility rate of verasper moseri germ cell |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494207A (en) * | 2015-12-29 | 2016-04-20 | 大连天正实业有限公司 | Wild verasper moseri domestication method |
| CN109892260A (en) * | 2019-04-19 | 2019-06-18 | 威海泰丰海水育苗有限公司 | A kind of separation method of round spot halibut superiority and inferiority matter fertilized eggs |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100388880C (en) * | 2005-02-01 | 2008-05-21 | 中国水产科学研究院黄海水产研究所 | Method for obtaining high quality fertilized egg of scophthatmus maximus |
| CN1922991B (en) * | 2006-09-22 | 2010-04-07 | 大连天正实业有限公司 | Seedling breeding method for dollar spot Scophthalmusmaximus |
-
2007
- 2007-07-11 CN CN2007100152860A patent/CN101341856B/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105494207A (en) * | 2015-12-29 | 2016-04-20 | 大连天正实业有限公司 | Wild verasper moseri domestication method |
| CN109892260A (en) * | 2019-04-19 | 2019-06-18 | 威海泰丰海水育苗有限公司 | A kind of separation method of round spot halibut superiority and inferiority matter fertilized eggs |
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| Publication number | Publication date |
|---|---|
| CN101341856B (en) | 2010-09-15 |
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Granted publication date: 20100915 Termination date: 20130711 |