CN101289661B - 用固相分离和纯化核酸分子 - Google Patents

用固相分离和纯化核酸分子 Download PDF

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Publication number
CN101289661B
CN101289661B CN200810092951.0A CN200810092951A CN101289661B CN 101289661 B CN101289661 B CN 101289661B CN 200810092951 A CN200810092951 A CN 200810092951A CN 101289661 B CN101289661 B CN 101289661B
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solid phase
nucleic acid
nucleic acids
adsorption
dna
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CN101289661A (zh
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C·伯克纳
H·冯德埃尔茨
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
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  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CN200810092951.0A 2007-04-20 2008-04-18 用固相分离和纯化核酸分子 Active CN101289661B (zh)

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Application Number Priority Date Filing Date Title
EP07008073 2007-04-20
EP07008073.4 2007-04-20

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CN101289661A CN101289661A (zh) 2008-10-22
CN101289661B true CN101289661B (zh) 2013-08-21

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CN200810092951.0A Active CN101289661B (zh) 2007-04-20 2008-04-18 用固相分离和纯化核酸分子

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US (1) US8101744B2 (https=)
EP (1) EP1983051B1 (https=)
JP (2) JP5325448B2 (https=)
CN (1) CN101289661B (https=)
CA (1) CA2629589C (https=)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE551433T1 (de) * 2005-05-06 2012-04-15 Gen Probe Inc Verfahren und produkte zum erfassen von nukleinsäurezielen
US10316352B2 (en) 2008-05-13 2019-06-11 Gen-Probe Incorporated Methods of capturing a target nucleic acid for amplification and detection using an inactivatable target capture oligomer
ES2565335T3 (es) * 2009-08-17 2016-04-04 Merck Patent Gmbh Método para aislar virus
US9371509B2 (en) 2009-09-02 2016-06-21 Roche Diagnostics Operations, Inc. Reagents for lysis of bacterial cells
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
US9024008B2 (en) 2010-07-07 2015-05-05 Diagon Ltd. Procedure for the specific isolation of total DNA content of bacterial germs and a kit for this purpose
US20150045232A1 (en) * 2010-12-28 2015-02-12 Bexmart Integrated and versatile methods for systems diagnosis of diseases
CN102533725A (zh) * 2011-09-09 2012-07-04 上海佑科生物技术有限公司 一体化缓冲液及使用该一体化缓冲液分离核酸的方法
US9340828B2 (en) 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
SG11201606921PA (en) 2014-01-28 2016-10-28 Dice Molecules Sv Llc Monoliths with attached recognition compounds, arrays thereof and uses thereof
JP2016020826A (ja) * 2014-07-14 2016-02-04 山善株式会社 分取液体クロマトフラフィ用インジェクタ
WO2018156906A1 (en) * 2017-02-27 2018-08-30 miDiagnostics NV System and method for purifying and amplifying nucleic acids
EP4010488A1 (en) * 2019-08-08 2022-06-15 Biocartis NV Novel nucleic acid purification chemistry
EP4159855A4 (en) * 2020-05-25 2024-07-24 Sekisui Medical Co., Ltd. METHOD FOR PURIFICATION OF NUCLEIC ACID
CN114573483B (zh) * 2022-03-16 2022-11-01 湖南师范大学 一种疏水的磁性离子液体及其制备方法和应用
CN114990109B (zh) * 2022-06-21 2025-03-25 中国科学院过程工程研究所 一种核糖核酸纯化伴侣及其应用
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
CN117434180A (zh) * 2023-10-27 2024-01-23 苏州百因诺生物科技有限公司 可提高细胞裂解液中质粒dna分离度的液相色谱检测方法及质粒dna浓度的检测方法
WO2025164403A1 (ja) * 2024-01-30 2025-08-07 株式会社AdvanSentinel 核酸の回収方法及びそれに用いられるキット

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005064010A1 (de) * 2003-12-19 2005-07-14 Preanalytix Gmbh Zusammensetzung zum binden von nukleinsäure an eine festphase
CN1680421A (zh) * 2004-02-20 2005-10-12 霍夫曼-拉罗奇有限公司 在核酸固相上的吸附
CN1781931A (zh) * 1997-12-06 2006-06-07 Dna研究创新有限公司 核酸的分离

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
EP0281390B1 (en) 1987-03-02 1994-06-22 Gen-Probe Incorporated Polycationic supports for nucleic acid purification, separation and hybridization
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
ATE138106T1 (de) 1988-07-20 1996-06-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
JPH06501225A (ja) 1990-07-09 1994-02-10 ジェイ　ガズデン　ピーティーワイ　リミテッド 改良型容器
CA2170604C (en) 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
BR9815569A (pt) * 1997-12-06 2001-10-09 Dna Res Instr Ltd Método para extração de biomoléculas de material biológico
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP0969090A1 (en) 1998-05-27 2000-01-05 QIAGEN GmbH Rapid and simple process for isolation of circular nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
DE60125035T2 (de) 2000-06-02 2007-06-21 Pall Corp. Behandlung von plasmid-haltigen lösungen
US6858720B2 (en) * 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US20040121336A1 (en) 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US20040180445A1 (en) 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
JPWO2005090563A1 (ja) * 2004-03-23 2008-02-07 大野　弘幸 核酸溶解用溶媒、核酸含有溶液および核酸保存方法
JP2009526970A (ja) * 2006-02-13 2009-07-23 エージェンシー フォー サイエンス，テクノロジー アンド リサーチ 生物学的試料または化学的試料の処理方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1781931A (zh) * 1997-12-06 2006-06-07 Dna研究创新有限公司 核酸的分离
WO2005064010A1 (de) * 2003-12-19 2005-07-14 Preanalytix Gmbh Zusammensetzung zum binden von nukleinsäure an eine festphase
CN1680421A (zh) * 2004-02-20 2005-10-12 霍夫曼-拉罗奇有限公司 在核酸固相上的吸附

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Berensmeier Sonja.Magnetic particles for the seqaration and purification of nucleic acids.《Applied microbiology and biotechnology》.2009,第73卷(第3期), *

Also Published As

Publication number Publication date
JP5325448B2 (ja) 2013-10-23
US8101744B2 (en) 2012-01-24
JP2012255798A (ja) 2012-12-27
CA2629589A1 (en) 2008-10-20
CN101289661A (zh) 2008-10-22
CA2629589C (en) 2016-03-29
US20080319182A1 (en) 2008-12-25
JP2008263982A (ja) 2008-11-06
EP1983051A3 (en) 2008-12-03
EP1983051A2 (en) 2008-10-22
HK1125672A1 (en) 2009-08-14
JP5685227B2 (ja) 2015-03-18
EP1983051B1 (en) 2014-11-26

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