CA2629589C - Isolation and purification of nucleic acid molecules with a solid phase - Google Patents

Isolation and purification of nucleic acid molecules with a solid phase Download PDF

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Publication number
CA2629589C
CA2629589C CA2629589A CA2629589A CA2629589C CA 2629589 C CA2629589 C CA 2629589C CA 2629589 A CA2629589 A CA 2629589A CA 2629589 A CA2629589 A CA 2629589A CA 2629589 C CA2629589 C CA 2629589C
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Canada
Prior art keywords
nucleic acid
solid phase
dna
guanidinium
solution
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CA2629589A
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English (en)
French (fr)
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CA2629589A1 (en
Inventor
Christian Birkner
Herbert Von Der Eltz
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2629589A1 publication Critical patent/CA2629589A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA2629589A 2007-04-20 2008-04-16 Isolation and purification of nucleic acid molecules with a solid phase Active CA2629589C (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008073 2007-04-20
EP07008073.4 2007-04-20

Publications (2)

Publication Number Publication Date
CA2629589A1 CA2629589A1 (en) 2008-10-20
CA2629589C true CA2629589C (en) 2016-03-29

Family

ID=38290130

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2629589A Active CA2629589C (en) 2007-04-20 2008-04-16 Isolation and purification of nucleic acid molecules with a solid phase

Country Status (5)

Country Link
US (1) US8101744B2 (https=)
EP (1) EP1983051B1 (https=)
JP (2) JP5325448B2 (https=)
CN (1) CN101289661B (https=)
CA (1) CA2629589C (https=)

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AU2009246363B2 (en) 2008-05-13 2013-10-24 Gen-Probe Incorporated Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
ES2565335T3 (es) * 2009-08-17 2016-04-04 Merck Patent Gmbh Método para aislar virus
US9371509B2 (en) * 2009-09-02 2016-06-21 Roche Diagnostics Operations, Inc. Reagents for lysis of bacterial cells
US8420801B2 (en) * 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
BR112012031267A2 (pt) 2010-07-07 2015-10-13 Diagon Kft procedimento para o isolamento específico do conteúdo de dna integral dos germes bacterianos e kit essa finalidade
US20150045232A1 (en) * 2010-12-28 2015-02-12 Bexmart Integrated and versatile methods for systems diagnosis of diseases
CN102533725A (zh) * 2011-09-09 2012-07-04 上海佑科生物技术有限公司 一体化缓冲液及使用该一体化缓冲液分离核酸的方法
EP2771462A4 (en) * 2011-10-27 2015-04-08 Ge Healthcare Bio Sciences Ab PURIFICATION OF NUCLEIC ACIDS
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
CA2940526A1 (en) 2014-01-28 2015-08-06 Dice Molecules Sv, Llc Monoliths with attached recognition compounds, arrays thereof and uses thereof
JP2016020826A (ja) * 2014-07-14 2016-02-04 山善株式会社 分取液体クロマトフラフィ用インジェクタ
US20200063189A1 (en) * 2017-02-27 2020-02-27 miDiagnostics NV System and method for purifying and amplifying nucleic acids
CN114450419A (zh) * 2019-08-08 2022-05-06 拜奥卡蒂斯生物股份有限公司 新型核酸纯化化学物质
JP7723657B2 (ja) * 2020-05-25 2025-08-14 積水メディカル株式会社 核酸の精製方法
CN114573483B (zh) * 2022-03-16 2022-11-01 湖南师范大学 一种疏水的磁性离子液体及其制备方法和应用
CN114990109B (zh) * 2022-06-21 2025-03-25 中国科学院过程工程研究所 一种核糖核酸纯化伴侣及其应用
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物
CN117434180A (zh) * 2023-10-27 2024-01-23 苏州百因诺生物科技有限公司 可提高细胞裂解液中质粒dna分离度的液相色谱检测方法及质粒dna浓度的检测方法
WO2025164403A1 (ja) * 2024-01-30 2025-08-07 株式会社AdvanSentinel 核酸の回収方法及びそれに用いられるキット

Family Cites Families (24)

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
DE3850273T2 (de) 1987-03-02 1994-09-29 Gen Probe Inc Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure.
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
WO1992000880A1 (en) 1990-07-09 1992-01-23 S.C.I. Operations Pty Limited Trading As Smorgon Plastics An improved container
AU689815B2 (en) 1993-08-30 1998-04-09 Promega Corporation Nucleic acid purification compositions and methods
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
GB9725839D0 (en) * 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
WO1999029703A2 (en) 1997-12-06 1999-06-17 Dna Research Instruments Limited Isolation of nucleic acids
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
EP0969090A1 (en) 1998-05-27 2000-01-05 QIAGEN GmbH Rapid and simple process for isolation of circular nucleic acids
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
WO2001094573A2 (en) 2000-06-02 2001-12-13 Pall Corporation Processing of plasmid-containing fluids
US6858720B2 (en) 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US20040121336A1 (en) 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US20040180445A1 (en) 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
WO2005064010A1 (de) 2003-12-19 2005-07-14 Preanalytix Gmbh Zusammensetzung zum binden von nukleinsäure an eine festphase
MXPA05001815A (es) * 2004-02-20 2005-08-24 Hoffmann La Roche Adsorcion de acidos nucleicos a una fase solida.
US20070196826A1 (en) 2004-03-23 2007-08-23 Hiroyuki Ohno Solvent For Dissolving Nucleic Acid, Nucleic Acid-Containing Solution And Method Of Preserving Nucleic Acid
CN101371124B (zh) * 2006-02-13 2012-02-29 新加坡科技研究局 处理生物样品和/或化学样品的方法

Also Published As

Publication number Publication date
JP5325448B2 (ja) 2013-10-23
CN101289661B (zh) 2013-08-21
JP5685227B2 (ja) 2015-03-18
CA2629589A1 (en) 2008-10-20
EP1983051A2 (en) 2008-10-22
US20080319182A1 (en) 2008-12-25
EP1983051B1 (en) 2014-11-26
HK1125672A1 (en) 2009-08-14
JP2008263982A (ja) 2008-11-06
EP1983051A3 (en) 2008-12-03
JP2012255798A (ja) 2012-12-27
CN101289661A (zh) 2008-10-22
US8101744B2 (en) 2012-01-24

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