CN101249097A - Applications of ash tree flowers polysaccharide for preparing preventing and controlling pig virosis medicament - Google Patents
Applications of ash tree flowers polysaccharide for preparing preventing and controlling pig virosis medicament Download PDFInfo
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- CN101249097A CN101249097A CNA2008100526613A CN200810052661A CN101249097A CN 101249097 A CN101249097 A CN 101249097A CN A2008100526613 A CNA2008100526613 A CN A2008100526613A CN 200810052661 A CN200810052661 A CN 200810052661A CN 101249097 A CN101249097 A CN 101249097A
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Abstract
The invention relates to the use of polysaccharides of Grifola frondosa in preparing drugs for controlling viral diseases in pigs, particularly to the use for controlling porcine rotavirus enteritis, porcine epidemic diarrhea and porcine transmissible gastroenteritis. For use, polysaccharides of Grifola frondosa are injected intramuscularly at a dose of 1.0-50 mg/kg, twice daily or mixed uniformly with a premix feed at a weight percentage of 0.1-10%. The premix feed is commercially available and pigs are fed ad libitum. The invention has the advantages that: the invention provide a new natural medicine for controlling viral diseases in pigs and improving porcine immunity in clinic; the natural medicine has high safety, definite effectiveness and controllable quality; and the natural medicine has easily-accessible raw materials, no toxicity and side effect and no risk of inducing drug resistance.
Description
Technical field
The present invention relates to the purposes of grifolan, the application of particularly a kind of grifolan in preparation control pig virus disease medicament.
Background technology
In pig disease, the loss that viral infection causes the pig aquaculture is that tool is big.In large scale of pig farm was produced, because of all multifactor influences, the sickness rate of viral diseases such as pig parvoviral, swine fever virus, pig blue-ear disease poison was up to 5～50%, and case fatality rate has caused very big economic loss for the production of raising pigs up to 1%～10%.For example, in causing the various causes of disease of diarrhea of pigs disease, the harm of viral diarrhea is the most serious, it can cause piglet death, become the losing flesh of pig, the price of deed reduction, increase the spending of labour cost and expenses for medicine.Transmissible gastroenteritis of swine, porcine epizootic diarrhea, porcine rotavirus are the main pathogen of China's epidemic virus diarrhea, other viruses also can cause the diarrhoea of pig as enterovirus infection, porcine adenovirus infection, parvovirus, Pseudorabies virus, swine fever virus simultaneously, we can say, the cause of disease of viral diarrhea is very complicated, prevent and treat viral diarrhea also the difficulty.When control pig virus disease, adopt antibiotic more, ediblely brought great diet hidden danger but antibiotic abuse is given the pig product, drug residue has become one of principal element that influences the pig product safety.
How to find a kind of viral pathogen is had directly kill or inhibitory action, reach by the intrinsic antipathogen mechanism of body (as immune system, complement system etc.) of regulating pig simultaneously that to press down viricidal effect be a problem that needs to be resolved hurrily.
Grifola frondosa is a kind of natural fungus of preciousness, has another name called hen of the woods, thousand Buddhist bacterium, Semen Castaneae mushroom, YUNTAN, Flos Nelumbinis bacterium etc.Contain the various active material in the Grifola frondosa, grifolan is a wherein topmost active component, has the component of remarkable physiologically active comprising D-component, MD-component, grifolan, X-component, MT-2 and LELFD etc.Grifolan is a kind of effective biological immunomodulator, and its different component all contains β-1,3-, the structure of β-1,6-glucosan, and active cell immunologic function greatly, thus human body immunity improving power has antitumor action.Grifolan may have direct inhibition virus, stimulates body immune system opposing virus and enhancing body to resist the ability of secondary disease simultaneously.The Grifola frondosa antiviral pathway has: directly suppress virus; Improve patient's autoimmunity; Reduce body secondary complication.The polysaccharide that extracts in the Grifola frondosa enters the internal energy raising activity of immune cells of human body, promotes the synthetic of interferon, produces antiviral protein in the inducing cell, reaches the effect that kills and wounds virus.At present, drug effect, the pharmacological research of grifolan antitumor action are a lot, and grifolan is as antitumor drug production and selling in market.But the research and the research and development of products that are used to prevent and treat the pig virus disease about grifolan in clinical yet there are no report.
Therefore, the application of exploitation grifolan in preparation control pig virus disease medicament is one of this technical field scientific research personnel new problem of being badly in need of developing.
Summary of the invention
The objective of the invention is to overcome the weak point in the above-mentioned technology, the application of a kind of grifolan in preparation control pig virus disease medicament is provided.
Implementation of the present invention is as follows for achieving the above object:
The application of a kind of grifolan in preparation control pig virus disease medicament especially is included in the application in control porcine rotavirus enteritis, porcine epizootic diarrhea and the transmissible gastroenteritis of swine.
In the application, give the pig muscle injection grifolan, every day 2 times, observe prevention effect by 1.0～50mg/Kg.Perhaps will contain grifolan and be by weight percentage 0.1～10% ratio and evenly admix in the premix material, premix material is common commercially available premix material, allows the pig free choice feeding observe prevention effect.
The invention has the beneficial effects as follows: through our clinical research confirmation, grifolan has excellent curative to the pig virus disease, especially can remove the clinical symptoms that viral infection causes, the mortality rate that reduces sick pig, promotes that the disease pig recovers to search for food; Evident in efficacy, safe and reliable, have no side effect, the treatment cost is low.
The specific embodiment
Below in conjunction with embodiment, to details are as follows according to the specific embodiment provided by the invention:
Embodiment 1
The research of grifolan treatment porcine rotavirus enteritis
Material and method
Experimental animal, grouping and test method
According to clinical observation and laboratory diagnosis, select 40 of following piglets through being diagnosed as rotaviral enteritis, be divided into treatment group and matched group, two groups to suffer from age pig year, clinical manifestation and state of an illness weight unanimous on the whole.
Each 20 example of treatment group matched group, the treatment group is pressed 5.0mg/Kg intramuscular injection grifolan, each 0.5ml/kg, every day 2 times.It is oral that matched group gives dioctahedral smectite, each 0.01～0.15g/kg, every day 2 times.Observe the mental status, body temperature, feces shape and color and luster and the piglet suckling or the situation of searching for food of piglet after the administration every day.
Efficacy determination
Clinical symptom disappearance behind sick pig medication 3～4d, feces is shaped, and body temperature, appetite, the normal person of the mental status are judged to healing; Feces retrogradation behind sick pig medication 3～4d, color and luster is normal, and appetite, the mental status take a turn for the better, and the normal person of body temperature is judged to improvement; Take a turn for the better and cure and all be judged to effectively; Still diarrhoea behind sick pig medication 3～4d, lassitude, detest the trencherman be judged to invalid.
Application result
Grifolan treatment rotaviral enteritis observation of curative effect
Table 1 liang group therapeutic outcome comparison (example, %)
Treatment group cure rate 40.0%, matched group cure rate 20.0%.Through the t check, two groups of cure rates are compared, and difference has statistical significance (P＜0.05).Illustrate that grifolan treatment rotaviral enteritis has significant curative effect.
Embodiment 2
The experimental study of grifolan control porcine epizootic diarrhea
1 experimental animal, grouping and test method
1.1 all support the feeding and management of test pig or the cement flooring is flat supports in the childbirth drylot feeding of the same terms with its sow for the examination piglet, routine disinfection is 1 time weekly.
1.2 therapeutic test is according to clinical observation and laboratory inspection, select 45 of following 1～25 age in days pigs through being diagnosed as porcine epizootic diarrhea, with the piglet of different days by disease time, symptom, nest not, body weight, sex, principle of reciprocity is divided into three groups of A, B, C at random.A group piglet is pressed 8.0mg/Kg intramuscular injection grifolan; B, C the group piglet by the medicine recommended dose respectively intramuscular injection give normal saline, astragalus polysaccharides.Three groups of medications every day once, logotype 5d.Observe the mental status of piglet every day after the administration, body temperature, feces shape and color and luster and piglet suckling or the situation of searching for food.
1.3 efficacy determination
Clinical symptom disappearance behind sick pig medication 3～4d, feces is shaped, and body temperature, appetite, the normal person of the mental status are judged to healing; Feces retrogradation behind sick pig medication 3～4d, color and luster is normal, and appetite, the mental status take a turn for the better, and the normal person of body temperature is judged to improvement, takes a turn for the better and cure all to be judged to effectively; Still diarrhoea behind sick pig medication 3～4d, lassitude, detest the trencherman be judged to invalid.Date processing carries out significance test to test group and the every data of matched group.
2 therapeutic tests
By table 2 as seen, grifolan reaches 86.7% to piglet epidemic diarrhea effective percentage, is significantly higher than the normal saline matched group.Through the t check, two groups of effective percentage are compared, and difference has statistical significance (P＜0.05).Illustrate that grifolan treatment porcine epizootic diarrhea has significant curative effect.
Table 2 grifolan to the therapeutic effect of 1～25 age in days piglet epidemic diarrhea in 120h (example, %)
Embodiment 3
The example of grifolan treatment transmissible gastroenteritis of swine
March in 2008 Tianjin plant on the 5th piglet, same symptoms appears successively: diarrhoea, heating, lassitude, detest the trencherman, found later on that the pig mortality rate rises on March 8, feed intake descends fast, dead 1～2 of per 100 pig every days, about 6/day of the most serious death.
At above-mentioned phenomenon, the grifolan intramuscular injection is used in suggestion, press 100mg/ pig use, and logotype 3～5 days cooperates multidimensional electrolyte etc. can control death rapidly in addition, the swinery state that refreshes, and invention rate and mortality rate obviously reduce.
Analyze: through cuing open inspection diagnosis and pathological diagnosis: transmissible gastroenteritis of swine.
Sum up: similar symptom, early find to carry out intramuscular injection by a grifolan 100mg/ pig by early treatment, once a day, logotype 3～5 days.Facts have proved that the grifolan may command transmissible gastroenteritis of swine state of an illness reduces mortality rate.
Embodiment 4
The using method of grifolan of prevention pig virus diarrhoea is: with grifolan is that 0.1～10.0% ratio is evenly admixed in the premix material by weight percentage, and premix material is for premix material commonly used, and is on sale on the market.Allow pig free choice feeding, observation prevent viral swine diarrheal effect.
Animal experiment: pig virus diarrhoea takes place in pig farm, Ninghe County, Tianjin throughout the year, and is poor through containing antibiotic feedstuff preventive effect such as ciprofloxacin lactate, so use the feedstuff that contains grifolan to be used to prevent pig virus diarrhoea.The piglet of 90 20 ages in days on this pig farm is divided into three groups of A, B, C, and 30 every group have been carried out 10 days prevention diarrhea of pigs contrast tests.The A group is admixed a kind of green premixing material of preventing and treating piglet diarrhea of embodiment 4 preparations by weight 0.1% ratio in feedstuff, the B group is admixed ciprofloxacin lactate by weight 0.1% ratio in feedstuff, does not contain the premix material of medicine in the C group feedstuff.Result of the test is that the protective rate of A group reaches 100.0%, and the B group only is 84.0%, and the C group only is 61.0%.The result shows that grifolan can effectively be prevented and treated pig virus diarrhoea.
Grifolan mainly has significant advantage to treatment pig virus prevention and treatment of diseases.Studies confirm that: grifolan has remarkable therapeutical effect to viral diseases such as transmissible gastroenteritis of swine, porcine rotavirus diseases, and pig virus diarrhoea is had preventive effect.As seen, grifolan is the natural drug of control pig virus disease.
Grifolan is a kind of green natural, noresidue harm, meets the natural drug of the theory of green health.It had both had pathogen directly kills or inhibitory action, simultaneously by regulating the intrinsic antipathogen mechanism of animal body (as immune system, complement system etc.) but reach the effect of killing the virus.
Above-mentioned with reference to the embodiment detailed description that the application in preparation control pig virus disease medicament is carried out to this grifolan; be illustrative rather than determinate; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (3)
1, the application of a kind of grifolan in preparation control pig virus disease medicament especially is included in the application in control porcine rotavirus enteritis, porcine epizootic diarrhea and the transmissible gastroenteritis of swine.
2, the application of grifolan according to claim 1 in preparation control pig virus disease medicament; Give the pig muscle injection grifolan by 1.0～50mg/Kg, every day 2 times, observe prevention effect.
3, the application of grifolan according to claim 1 in preparation control pig virus disease medicament; To contain grifolan and be by weight percentage 0.1～10% ratio and evenly admix in the premix material, premix material is common commercially available premix material, allows the pig free choice feeding, observes prevention effect.
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| CN2008100526613A CN101249097B (en) | 2008-04-08 | 2008-04-08 | Applications of ash tree flowers polysaccharide for preparing preventing and controlling pig virosis medicament |
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| CN2008100526613A CN101249097B (en) | 2008-04-08 | 2008-04-08 | Applications of ash tree flowers polysaccharide for preparing preventing and controlling pig virosis medicament |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695338B (en) * | 2009-10-26 | 2012-07-25 | 寇跃庆 | Method for producing feed additive for reducing livestock cholesterol |
| CN103860580A (en) * | 2014-03-27 | 2014-06-18 | 华夏先葆(北京)中药研究院有限公司 | Compound fungal polysaccharide preparation having functions of supporting healthy energy, strengthening spleen and stomach and boosting immunity |
| CN113651900A (en) * | 2021-10-10 | 2021-11-16 | 吉林农业大学 | Grifola frondosa purified polysaccharide with blood lipid regulating function and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100419191B1 (en) * | 2003-06-23 | 2004-02-19 | 주식회사 엔바이오테크놀러지 | Composition comprising soluble glucan oligomer from Saccharomyces cerevisiae IS2 inhibiting the swine influenza (SIV) and transmissible gastroenteritis coronavirus (TGEV) virus |
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2008
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101695338B (en) * | 2009-10-26 | 2012-07-25 | 寇跃庆 | Method for producing feed additive for reducing livestock cholesterol |
| CN103860580A (en) * | 2014-03-27 | 2014-06-18 | 华夏先葆(北京)中药研究院有限公司 | Compound fungal polysaccharide preparation having functions of supporting healthy energy, strengthening spleen and stomach and boosting immunity |
| CN103860580B (en) * | 2014-03-27 | 2017-11-28 | 华夏先葆(北京)中药研究院有限公司 | It is a kind of have strengthen the body resistance to consolidate the constitution, the composite fungi amylose preparation of strengthening the spleen and stomach, strengthen immunity function |
| CN113651900A (en) * | 2021-10-10 | 2021-11-16 | 吉林农业大学 | Grifola frondosa purified polysaccharide with blood lipid regulating function and preparation method thereof |
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| CN101249097B (en) | 2011-09-07 |
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