CN103461676B - Feed additive comprising lysozyme, pesticide composition and application of feed additive - Google Patents
Feed additive comprising lysozyme, pesticide composition and application of feed additive Download PDFInfo
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Abstract
The invention relates to a feed additive comprising lysozyme, a pesticide composition and application of the feed additive. The lysozyme, compound grease and a traditional Chinese medicine composition are combined for use, the dosage of the lysozyme is controlled and the additive amount in the feed is regulated correspondingly; due to the synergistic effect of the lysozyme, the compound grease and the traditional Chinese medicine composition, by utilizing good antibacterial and sterilizing effects of the lysozyme and the immunoregulation, free radical regulation and assisting effect of the compound grease and the traditional Chinese medicine composition on an organism, the organism is regulated from the inside to the outside, so that the feed additive and the pesticide composition can resist inflammation and infection effectively and improve autoimmunity and resistance to diseases, also can kill strains having tolerance to antibiotic, guarantees the health of intestinal tracts of animals, prevents and cures animal diarrhea, and improves the production performance of animals. The feed additive and the pesticide composition are good in curative effect, high in safety and low in cost, have no drug resistance, no drug residue and no toxic and side effects, and are environment-friendly active substances.
Description
Technical field
The present invention relates to a kind of additive for farm animal feed and pharmaceutical composition, relate in particular to a kind of feed addictive that contains lysozyme and uses thereof.
Background technology
Grice diarrhoea is the typical multi-factor disease of the one in Swine Production, and according to statistics, the incidence of disease of grice diarrhoea is 35%, and its indirect loss shows that again healing Piglet Development is slow, and feed conversion rate is low, the development of serious threat pig industry.Since in May, 2006, priority eruption and prevalence porcine hyperthermia, causes large quantities of pig Mortalities throughout the country, causes great economic loss.So far, hyperpyrexia disease still occurs popular in the swinery in 22 provinces, the whole nation, and the healthy growth of harm pig, is threatening the development of pig industry.The adverse consequences that the use of antibiotic in cultivation brings is all fully realized.Many experts think in the whole nation hypoimmunity that major reason is pig in a lot of areas pandemic " porcine hyperthermias ", and premunition is low, is a major reason that causes pig immunity low and chronically, heavy dose ofly use antibiotic.
In prior art, disclose lysozyme and be used for the treatment of animal infection, CN101612393A discloses the application process of a kind of microbic muramidase in medicine and the additive of prevention pig disease, is combined with by lysozyme or with fluorine Ben Nikao, reaches result for the treatment of; CN101611765A reaches the effect of prevention, treatment grice diarrhoea by adding the lysozyme of higher dosage.But research is before limited for the utilization means of lysozyme, lysozyme use amount is high, cost is higher, and the antibiotic use such as fluorine Ben Nikao has impact for the recovery of body.Research is also found, probio in animal body has certain tolerance to lysozyme, and under 25mg/kg concentration, lysozyme can not affect lactobacillus thermophilus and streptococcic growth, if but exceeded the tolerance concentration range of probio, molten mattress enzyme would just have certain inhibitory action to probio.Thus, lysozyme drug effect how to improve prior art is to need the problem that solves.
Summary of the invention
The object of the present invention is to provide a kind of feed addictive that contains lysozyme, medical composition and its use, utilize this additive can effectively reduce the use amount of lysozyme, reduce ill-effect, under antibiotic prerequisite, obtain better antibacterial effect not using, safer, effectively to treat pig infection, the effect that simultaneously reach growth promotion, improves pig immunity, infection and recurrence rate is low.
The technical solution used in the present invention is as follows to achieve these goals:
A kind of animal feed additive provided by the present invention, contains lysozyme in described additive, and concentration is 25-300ppm, also contains the compound grease of additive total amount 1-10% and 1-5% Chinese medicine composition in additive.
Described compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1:2～1:20.
It is that raw material is made that described Chinese medicine composition adopts the bighead atractylodes rhizome, the root of kudzu vine, the capsule of weeping forsythia, brook anemone, hawthorn and kuh-seng, and the weight portion of described Chinese medicine composition is bighead atractylodes rhizome 10-30 part, root of kudzu vine 10-18 part, capsule of weeping forsythia 5-15 part, brook anemone 1-8, hawthorn 1-8 part, kuh-seng 1-8 part.
Take the bighead atractylodes rhizome, the root of kudzu vine, the capsule of weeping forsythia, brook anemone, hawthorn and the kuh-seng of required weight portion, ultramicro grinding after mixing, crosses 100-200 mesh sieve, adds in feed addictive or medicine in required ratio.
Described animal is livestock and poultry, is preferably domestic animal, more preferably pig, piglet.
In order to reach good effect, reduce costs simultaneously, preferred animal feed additive contains 50ppm lysozyme, 3% compound grease and 2% Chinese medicine composition; Compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1:10; Bighead atractylodes rhizome 10-30 part, root of kudzu vine 10-18 part, capsule of weeping forsythia 5-15 part, brook anemone 1-8, hawthorn 1-8 part, kuh-seng 1-8 part.
The application also provides a kind of prevention or has treated the pharmaceutical composition of swine dysentery, porcine hyperthermia, is made up of the lysozyme that bulk drug is 50ppm, 3% compound grease and 2% Chinese medicine composition bulk drug and pharmaceutically acceptable carrier; Described compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1:10; Described Chinese traditional medicine composition raw material is 25 parts of the bighead atractylodes rhizomes, 14 parts of the roots of kudzu vine, 11 parts of the capsules of weeping forsythia, brook anemone 2,3 parts of hawthorn, 2 parts of kuh-sengs.
Lysozyme be can dissolution of bacteria a kind of enzyme of cell membrane, be a class material general name.As the important member of nospecific immunity, lysozyme is playing an important role aspect the invasion of defence inoculating microbe, and in its immunocyte animal and macrophage, content is higher.Bacterium, hay bacillus, micrococcus radiodurans that lysozyme forms gram-positive bacteria, aerobic spermatium all have good decomposition, and the Gram-negative bacterias such as Escherichia coli, salmonella and secondary courageous and upright vibrios are also had to dissolution.Based on this, the present invention makes an addition to lysozyme in feed addictive, prevents and treat the bacteriosis of pig.
DHA is conventional nervous system cell growth factor, but the application finds unexpectedly, DHA has synergistic effect for the drag aspect of lysozyme amelioration of inflammation, raising, may be the formation that can suppress inflammation front wheel driving material due to DHA, thus performance dependent interaction; Linseed oil is rich in alpha-linolenic acid, DHA and EPA, and interpolation linseed oil can reduce the consumption of DHA, reduces costs; In addition, DHA, linseed oil can provide body required polyunsaturated fatty acid, such as Omega3 aliphatic acid, can reduce body Free Radical Level, reach the effect that improves Abwehrkraft des Koepers.
That Rhizoma Atractylodis Macrocephalae, the root of kudzu vine, the capsule of weeping forsythia, brook anemone, hawthorn and kuh-seng have is clearing heat and detoxicating, strengthening the spleen and stomach, promoting blood circulation and removing obstruction in channels, constitutional effect, not only reach the object of supplemental treatment, and strengthen digestive system, circulatory function, and alleviate rear body injury, strengthen domestic animals premunition.
The invention provides described feed addictive for the preparation of prevention or treat the purposes in animal infected feed; Provide described pharmaceutical composition for the preparation of prevention or treat the purposes in animal infected medicine.
Described zoogenetic infection is porcine hyperthermia or swine dysentery.
Feed addictive of the present invention, makes an addition in feed with 0.5～20% ratio, feeds for feed routine; Also can be directly oral.
Beneficial effect of the present invention is as follows:
1, the present invention effectively treats bacteriosis, swine dysentery and the hyperpyrexia disease of pig, and relief of symptoms significantly improves the immunity of pig, slows down stress reaction, improves and safeguards gastrointestinal bacterial flora balance.Good effect, safe, with low cost, have no drug resistance, drug residue free, have no side effect, be the active material of environmental protection.
2, by lysozyme, compound grease and Chinese medicine composition three's synergy, the good restraining and sterilizing bacteria effect of utilizing lysozyme to have, compound grease, Chinese medicine composition regulate, improve the effect of resistance and auxiliary anti-inflammatory for immunological regulation, the free radical of body, adjust from inside to outside body, can be effectively to anti-inflammatory, infection, strengthen self resistance, disease resistance, the bacterial strain that antibiotic has been produced to drug resistance also has very strong killing action, ensure the health of the enteron aisle of animal, control animal diarrhea, improves breeding performonce fo animals.
3, the perfect use of lysozyme, by using with other active component compatibilities, has effectively reduced the consumption of lysozyme, and has reached better result for the treatment of.
4, adopt positive therapy, side effect is low, and excitating organism immune level in treatment reaches the effect for the treatment of and improving disease resistance by the raising of autoimmunity, and feed morbidity animal and susceptible animal can reach prevention and result for the treatment of simultaneously simultaneously.
5, lysozyme is in food industry, to be good natural antiseptic agent, and gram positive bacteria, hay bacillus, micrococcus radiodurans etc. are all had to powerful molten wall effect.The Gram-negative bacterias such as Escherichia coli, proteus vulgaris and vibrio parahaemolytious etc. are also had to certain dissolution, be added on and in feed, can not destroy the nutritional labeling in feed, in feed, adding lysozyme can prevent from going mouldy, extend the storage period of feed, reduce the probability of taking in the feed that goes mouldy, from source prevention zoogenetic infection.
Detailed description of the invention
Embodiment mono-: the bacteriostatic test of lysozyme
The requirement of the application's lysozyme used is: by drug sensitive test, in the time of 100ppm, should have obvious inhibition zone, i.e. the diameter > 10mm of inhibition zone.Susceptibility test methods is as follows: the drug sensitive test of doing different bacterium can be selected different culture mediums, as does colibacillary drug sensitive test and can select ordinary nutrient agar or the triumphant culture medium of wheat bran.Do salmonella and can select blood serum medium.
One, the preparation of general LB culture medium
1, the preparation of fluid nutrient medium
Peptone 1g, NaCl1g, yeast extract 0.5g (offshore company's product is selected in suggestion) are dissolved in 100ml water.
2, the preparation of solid medium
On the basis of liquid medium within, in every 100ml, add agar 1.7g or agar powder 2g.
Two, sterilizing
Culture medium, flat board, rifle head, cotton swab, physiological saline and cushioning liquid used in whole experimentation all need sterilizing.
Three, operating process
1, indicator bacteria (Escherichia coli, salmonella etc.) is inoculated in to dull and stereotyped activation culture from nutrient solution (base), after bacterium colony grows, on flat board, selects colonies typical and be inoculated in the fluid nutrient medium in test tube and cultivate after 4-6 hour than turbid.
2, with being coated with bacterium rod (or sterilized cotton swabs), bacterial suspension to be checked is applied in to plate media surface, requires coating even compact.
3, with sterile working by the stainless steel tubule of sterilizing (also useable glass pipe, porcelain tube), be placed on culture medium and punch, the culture medium in hole is chosen with syringe needle, and with flame back cover, make the culture medium can be fully and plate fusion (in case liquid seepage affects result).
4, the analyte sample fluid preparing 80 microlitres are added in sample hole, after sample adds, put into the incubator of 30-37 DEG C and cultivate observed result after 12-18 hour.
Attention: the process of test operates under aseptic condition.
Four, the preparation of lysozyme sample liquid
1, the preparation of pulverized specimen:
Getting the physiological saline stepwise dilution that the sterilizing of lysozyme sample is good is 400ppm to concentration gradient, 300ppm, and 200ppm, 100ppm, 50ppm, then 25ppm gets respectively the solution that 80 microlitres have diluted and is placed in sample well.(note: in the situation that temperature is lower, may occur insoluble situation a little, can be heated to 35-40 DEG C is solubilized)
2, points for attention
1) sample liquid configuring is not placed under freezing condition and preserves, and can not be placed on aseptic operating platform and be irradiated by ultraviolet ray;
2) sample liquid notes wanting stepwise dilution in prepared and diluted process.
In order to determine the addition of lysozyme in medicine and additive, we have carried out following drug sensitive experiment: select 400ppm, 300ppm, 200ppm, 100ppm, 50ppm, six concentration of 25ppm are tested, and test method is the same, taking dust Xi Shi Escherichia coli as test strain, detect the big or small check test result of inhibition zone by detection, concrete as table 1.
Table 1 bacteriostatic test result
As can be seen from Table 1, the lysozyme fungistatic effect of 25-100ppm is not obvious, and along with the increase of lysozyme concentration, fungistatic effect significantly increases, but, exceeding after 300ppm, fungistatic effect increases not obvious.
Embodiment 2: piglet dysentery test
(1) experiment of preventive effects design
28 age in days piglets are divided into 10 groups at random, take in 5g additive at every turn, every day 4 times.Be respectively control group (not adding feed addictive); Test group 1, test group 2, test group 3, test group 4, test group 5; Contrast groups 1, contrast groups 2, contrast groups 3, contrast groups 4; Establish three repeating groups for every group, 4 weeks experimental periods.The identical piglet of selection body condition of pre-anti-diarrhea, dysentery carries out challenge test, and its preventive effect is in table 2.
Test group 1: contain lysozyme in additive, concentration is 25ppm, also has the compound grease of additive total amount 3% and 2% Chinese medicine composition in additive; Described compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1:10; Chinese medicine composition is 25 parts of the bighead atractylodes rhizomes, 14 parts of the roots of kudzu vine, 11 parts of the capsules of weeping forsythia, brook anemone 2,3 parts of hawthorn, 2 parts of kuh-sengs.
Test group 2: contain lysozyme in additive, concentration is 50ppm, and other are with test group 1.
Test group 3: contain lysozyme in additive, concentration is 100ppm, and other are with test group 1.
Test group 4: contain lysozyme in additive, concentration is 200ppm, and other are with test group 1.
Test group 5: lysozyme not in additive, other are with test group 1.
Contrast groups 1-4 is respectively in CN101611765A detailed description of the invention for 1 group-4 groups of the tests of piglets.
(2) result for the treatment of experimental design
The pig that occurs dysentery is divided into 10 groups at random, takes in 20g, four times a day at every turn.Be respectively control group (not adding feed addictive); Test group 1, test group 2, test group 3, test group 4; Contrast groups a, contrast groups b, contrast groups c, contrast groups d; Establish three repeating groups for every group, 1 week experimental period,, its result for the treatment of is in table 2.
Contrast groups a: piglet only gavages the per day 0.5g of lysozyme formulation.
Contrast groups b: piglet only gavages the per day 1g of lysozyme enzyme preparation.
Contrast groups c: piglet only gavages the per day 2g of lysozyme enzyme preparation.
Contrast groups d:: piglet only gavages the per day 3g of lysozyme enzyme preparation.
(3) observation index and management
Observe each group of feed consumption rate, weightening finish, a diarrhoea number of times etc., and note down.Raise with a subfield, the pellet of feeding, divided meal is fed, every day four times (time is respectively 7:00,11:00,15:00,19:00), feeding piglet management routinely.
Table 2 prevents and treats diarrhoea situation
As can be seen from Table 2, the application has used the extremely low lysozyme consumption (feed addictive of the above lysozyme content of 50ppm, prophylactic tria day take in 20g additive and be equivalent to take in 1mg lysozyme, take in 80g additive therapeutic test day with respect to taking in 4mg lysozyme) reach significant prevention and treated the effect of suffering from diarrhoea, compared with the contrast groups 1-4 of equal lysozyme concentration, average diarrhea rate obviously reduces; Compared with contrast groups a-d, use the more lysozyme of low dosage, reach equivalent curative effect, average diarrhea rate obviously reduces; The test group 5 of not adding lysozyme has certain prevention effect, but prevention effect is all lower than the test group of adding lysozyme, illustrates that the use of compound grease and Chinese medicine composition makes the effect synergy of lysozyme, has reached collaborative prevention effect.
Embodiment 3: piglet hyperpyrexia disease test
At the early stage of pig farm hyperpyrexia disease, by the feed addictive of feeding experiment group 1-4, the feed that contains lysozyme by feeding can prevent the further deterioration of hyperpyrexia disease, and the disease control at hyperpyrexia disease initial stage the results are shown in Table 3.
The treatment results at table 3 hyperpyrexia disease initial stage
As can be seen from the above table, the feed addictive of the above lysozyme content of 50ppm has reached desirable control effect to the control of disease, and can find out alone 50ppm lysozyme from embodiment 1, and fungistatic effect is also not obvious.Visible, by compound grease and Chinese medicine composition and its synergy, use lower lysozyme to reach better result for the treatment of, effectively control the hyperpyrexia initial stage state of an illness.
Embodiment 4: Immunological Study
(1) improve immunity function test and appraisal zoopery
1, animal used as test NIH mouse 20-22g, single sex, SPF level, 40 every group, totally four groups.
2, dosage grouping and given the test agent give the time
Three groups of given the test agent and a negative control group are established in experiment, each gavage 1g.
(in additive, contain lysozyme, concentration is 50ppm to test group 2, also has the compound grease of additive total amount 3% and 2% Chinese medicine composition in additive; Described compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1:10; Chinese medicine composition is 25 parts of the bighead atractylodes rhizomes, 14 parts of the roots of kudzu vine, 11 parts of the capsules of weeping forsythia, brook anemone 2,3 parts of hawthorn, 2 parts of kuh-sengs);
Contrast groups e (300ppm lysozyme does not add compound grease and Chinese medicine composition);
Contrast groups f (do not add compound grease, other are with test group 2); If negative control group.Given the test agent is prepared by deionized water, and given the test agent gives 35 days time;
Negative control group is deionized water.
3, test method
Animal was quarantined after one week under laboratory condition, below each experiment be at random 40 mouse be divided into four groups, 10 every group, every day, gavage gave tested material, to sample amount, according to body weight, increase and decrease is adjusted weekly, experimental period is 4 weeks.
The mouse spleen lymphocyte transformation experiment (mtt assay) of 3.1ConA induction
Splenocyte suspension preparation: the aseptic spleen of getting, be placed in and fill appropriate aseptic Hank ' s liquid plate, gently spleen is ground with tweezers, make individual cells suspension.Filter through 200 eye mesh screens, or spleen is ground with 4 layers of gauze, wash 2 times each centrifugal 10min (1000r/min) with Hank ' s liquid.Abandoning supernatant upsprings cytoplasm, add 0.5mL aqua sterilisa 20 seconds, after splitting erythrocyte, add again 0.5mL2 times of Hank ' s liquid and 8mLHank ' s liquid, 1000r/min, 10min is centrifugal, then cell is suspended in the complete culture solution of 1mL, with counting after 1% glacial acetic acid dilution (viable count should more than 95%), adjusting cell concentration be 3～5 × 10
6individual/mL.
Lymphproliferation response: divide two holes to add in 24 well culture plates every a splenocyte suspension, every hole 1mL, a hole adds 75 μ L ConA liquid (being equivalent to 7.5 μ g/mL), and 5%CO in contrast, is put in another hole
2, 37 DEG C of CO
2in incubator, cultivate 72h.Cultivation finishes front 4h, and every hole sucks supernatant 0.7mL gently, adds 0.7mL not containing the RPM11640 nutrient solution of calf serum, adds MTT (5mg/mL) 50 μ L/ holes simultaneously, continues to cultivate 4h.After cultivation finishes, every hole adds 1mL acid isopropyl alcohol, and piping and druming mixes, and purple crystal is dissolved completely.Then divide and install in 96 well culture plates, 3 parallel holes are made in each hole, with ELIASA, measure OD value with 570nm wavelength.Also lysate directly can be moved in 2mL cuvette, on 721 spectrophotometers, measure OD value at wavelength 570nm.
3.2, mouse delayed allergy (the sufficient sole of the foot thickens method)
The 4th day immune animal before off-test and serum separate, by 2% sheep red blood cell (SRBC) lumbar injection 0.2ml sensitized animal, after 5 days, measure left back sufficient sole of the foot portion thickness, then after this place's hypodermic injection 20% (v/v) sheep red blood cell (SRBC) (20ul/ only) injection, 24h measures left back sufficient sole of the foot thickness three times, obtains mean value.
3.3, the mensuration of mice serum hemolysin
After last administration, dilute to obtain india ink with 1:4, inject the india ink of dilution by body weight from mouse tail vein
(10ml/kg), inject after prepared Chinese ink 2,10min, get blood 20 μ L from angular vein clump respectively, exist side by side and be added to 2mL0.1%Na by it
2cO
3in solution.With 721 spectrophotometers at 600nm wavelength place's photometry density value (OD), with Na
2cO
3solution is made blank.
3.4, NK cytoactive detection:
Splenocyte suspension must be prepared (effector cell): the aseptic spleen of getting, be placed in the little plate that fills appropriate aseptic Hank ' s liquid, and gently spleen is ground with tweezers, make single cell suspension.Shine net filtration through 200 orders.Hank ' s liquid is washed 3 times, each centrifugal 10min (1000r/min).Then cell being suspended in the cultivation completely of 2ml, with the blue dyeing counting viable count of platform phenol (should more than 95%), be finally 0.5 × 107/ml with RPMI1640 complete culture solution adjustment cell concentration.
NK cytoactive detects: getting concentration is 1 × 10
5the each 100ul of the target cell of individual/ml and effector cell (effect target is than 50:1), adds in U-shaped 96 well culture plates; Target cell Spontaneous release hole adds target cell and the each 100ul of nutrient solution, and the maximum release aperture of target cell adds the each 100ul of target cell and 1%NP40 or 2.5%Triton; Above-mentioned every three parallel holes of all establishing, in 37 DEG C, 5%CO
2in incubator, cultivate 4h, then by 96 well culture plates with the centrifugal 5min of 1500r/min, draw at the bottom of supernatant 100ul horizontalization in 96 well culture plates in every hole, add LDH matrix liquid 100ul simultaneously, according to room temperature differential responses 3～10min, every hole adds the HCl30ul of 1mol/L, measures OD value (OD) at ELIASA 490nm place.
Data processing: all data are all carried out variance analysis through SPSS statistical software.
4, experimental result
Table 4 strengthens immunity test result
The application's test group 2 can improve the mouse delayed allergy ability (the sufficient sole of the foot thickens method) of sheep red blood cell (SRBC) induction as can be seen from Table 4; The reaction of animal subject Hemolysin titre is increased, increase the NK cytoactive of animal subject.In addition, the use of control group f Chinese medicine can effectively improve the immunity of mouse, but effect is lower than also add the test group 2 that is rich in the compound grease of unrighted acid simultaneously, and result shows that the application's test group 2 has effect of significant enhancing immunity compared with other groups.
Claims (9)
1. an animal feed additive, in described additive, contain lysozyme, concentration is 50ppm, it is characterized in that, in additive, also contain the compound grease of additive total amount 3% and 2% Chinese medicine composition, described compound grease is DHA and linseed oil, and in compound grease, the ratio of DHA and linseed oil is 1: 10, and the weight portion of described Chinese medicine composition is 25 parts of the bighead atractylodes rhizomes, 14 parts of the roots of kudzu vine, 11 parts of the capsules of weeping forsythia, brook anemone 2,3 parts of hawthorn, 2 parts of kuh-sengs.
2. animal feed additive according to claim 1, is characterized in that, described animal is livestock and poultry.
3. animal feed additive according to claim 2, is characterized in that, described animal is domestic animal.
4. animal feed additive according to claim 3, is characterized in that, described animal is pig.
5. in claim 1 to 4, the animal feed additive described in any one prevents or treats the purposes in animal infected feed in preparation.
6. purposes according to claim 5, is characterized in that, described zoogenetic infection is porcine hyperthermia.
7. purposes according to claim 5, is characterized in that, described zoogenetic infection is swine dysentery.
8. according to the purposes one of claim 5 to 7 Suo Shu, it is characterized in that, the addition of described feed addictive in feed is 0.5-20%.
9. a pharmaceutical composition for prevention or treatment swine dysentery, porcine hyperthermia, is made up of bulk drug and pharmaceutically acceptable carrier, it is characterized in that, and in total composition, the lysozyme that bulk drug is 50ppm, 3% compound grease and 2% Chinese medicine composition; Described compound grease is DHA and linseed oil, and the ratio of DHA and linseed oil is 1: 10; Described Chinese traditional medicine composition raw material is 25 parts of the bighead atractylodes rhizomes, 14 parts of the roots of kudzu vine, 11 parts of the capsules of weeping forsythia, brook anemone 2,3 parts of hawthorn, 2 parts of kuh-sengs.
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| CN104920897A (en) * | 2015-06-30 | 2015-09-23 | 江西瑞普康恩农牧发展有限公司 | Feed used for piglets to increase daily weight gain and reduce diarrhea |
| CN113615773B (en) * | 2017-04-14 | 2023-07-25 | 浙江艾杰斯生物科技有限公司 | Feed additive containing lysozyme and traditional Chinese medicine extracts |
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| 恶痢停+生物溶菌酶对仔猪黄、白痢的防治;李蓉;《山东畜牧兽医》;20130515;第34卷(第5期);43-44 * |
| 李蓉.恶痢停+生物溶菌酶对仔猪黄、白痢的防治.《山东畜牧兽医》.2013,第34卷(第5期),43-44. |
| 沈彦萍.溶菌酶可溶性粉剂防治仔猪腹泻的效果观察.《饲料工业》.2005,第26卷(第6期),16-18. |
| 溶菌酶可溶性粉剂防治仔猪腹泻的效果观察;沈彦萍;《饲料工业》;20050320;第26卷(第6期);16-18 * |
| 溶菌酶对断奶仔猪生产性能的影响;邱伟海;《饲料博览》;20060210(第2期);39-41 * |
| 邱伟海.溶菌酶对断奶仔猪生产性能的影响.《饲料博览》.2006,(第2期),39-41. |
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