CN101139615B - 一种快速高效构建哺乳动物细胞rna干扰载体及其验证载体的方法 - Google Patents
一种快速高效构建哺乳动物细胞rna干扰载体及其验证载体的方法 Download PDFInfo
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- CN101139615B CN101139615B CN2007100529123A CN200710052912A CN101139615B CN 101139615 B CN101139615 B CN 101139615B CN 2007100529123 A CN2007100529123 A CN 2007100529123A CN 200710052912 A CN200710052912 A CN 200710052912A CN 101139615 B CN101139615 B CN 101139615B
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CN2007100529123A CN101139615B (zh) | 2007-08-06 | 2007-08-06 | 一种快速高效构建哺乳动物细胞rna干扰载体及其验证载体的方法 |
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CN101139615A CN101139615A (zh) | 2008-03-12 |
CN101139615B true CN101139615B (zh) | 2011-10-19 |
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Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
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US20110196142A1 (en) * | 2008-09-04 | 2011-08-11 | Biotechnology Research Institute The Chinese Academy of Agricultural Sciences | DNA molecule for expressing Hairpin RNA, the constructing method and the use thereof |
CN101857902A (zh) * | 2010-06-21 | 2010-10-13 | 西北农林科技大学 | 一种脂蛋白脂酶基因有效shRNA的筛选方法 |
CN103060374B (zh) * | 2012-10-30 | 2014-05-07 | 中国农业科学院兰州兽医研究所 | 一种基于位点特异性重组的rna干扰载体及其构建方法和应用 |
CN108251444B (zh) * | 2018-03-22 | 2022-09-27 | 复旦大学附属肿瘤医院 | 基于pcr的无缝构建小rna表达载体的方法 |
CN111434771A (zh) * | 2019-01-15 | 2020-07-21 | 四川大学 | 一种线性化载体自重组的质粒构建方法 |
CN110305885A (zh) * | 2019-05-08 | 2019-10-08 | 四川大学 | 利用ccdB致死基因快速构建重组质粒的试剂盒 |
CN111334507B (zh) * | 2019-12-24 | 2024-01-30 | 江苏农牧科技职业学院 | 绵羊Lrh-1短发夹RNA及其干扰载体 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1458279A (zh) * | 2003-06-05 | 2003-11-26 | 复旦大学 | 一种利用大肠杆菌发酵制备小干扰rna分子的方法 |
CN1539979A (zh) * | 2003-10-24 | 2004-10-27 | 中国人民解放军第二军医大学 | 具有绿色荧光蛋白示踪和抗性筛选标记的RNAi载体pGO |
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
CN1710079A (zh) * | 2005-04-27 | 2005-12-21 | 中国医学科学院医药生物技术研究所 | 一种可同时表达多种shRNA的载体pCSH1-n及其构建方法 |
CN1844403A (zh) * | 2006-04-13 | 2006-10-11 | 中国农业科学院植物保护研究所 | 正反向双启动子植物RNAi双元表达载体 |
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1458279A (zh) * | 2003-06-05 | 2003-11-26 | 复旦大学 | 一种利用大肠杆菌发酵制备小干扰rna分子的方法 |
CN1539979A (zh) * | 2003-10-24 | 2004-10-27 | 中国人民解放军第二军医大学 | 具有绿色荧光蛋白示踪和抗性筛选标记的RNAi载体pGO |
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
CN1710079A (zh) * | 2005-04-27 | 2005-12-21 | 中国医学科学院医药生物技术研究所 | 一种可同时表达多种shRNA的载体pCSH1-n及其构建方法 |
CN1844403A (zh) * | 2006-04-13 | 2006-10-11 | 中国农业科学院植物保护研究所 | 正反向双启动子植物RNAi双元表达载体 |
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Inventor after: Ma Lixin Inventor after: Yu Xiaolan Inventor after: Li Chunhua Inventor after: Sun Xiaoyan Inventor after: Xu Jun Inventor after: Zeng Jieqiong Inventor before: Ma Lixin Inventor before: Sun Xiaoyan Inventor before: Xu Jun Inventor before: Zeng Jieqiong Inventor before: Li Chunhua |
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