CN101092613B - 从亚油酸异构酶产生菌中提取纯化亚油酸异构酶的方法 - Google Patents
从亚油酸异构酶产生菌中提取纯化亚油酸异构酶的方法 Download PDFInfo
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- CN101092613B CN101092613B CN200710099653XA CN200710099653A CN101092613B CN 101092613 B CN101092613 B CN 101092613B CN 200710099653X A CN200710099653X A CN 200710099653XA CN 200710099653 A CN200710099653 A CN 200710099653A CN 101092613 B CN101092613 B CN 101092613B
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- linoleic acid
- acid isomerase
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- potassium phosphate
- phosphate buffer
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- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 title claims abstract description 83
- 102000004195 Isomerases Human genes 0.000 title claims abstract description 68
- 108090000769 Isomerases Proteins 0.000 title claims abstract description 68
- 235000020778 linoleic acid Nutrition 0.000 title claims abstract description 68
- 238000000034 method Methods 0.000 title claims abstract description 42
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 title description 5
- 102000004190 Enzymes Human genes 0.000 claims abstract description 74
- 108090000790 Enzymes Proteins 0.000 claims abstract description 74
- 239000000243 solution Substances 0.000 claims abstract description 60
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 32
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- 239000007788 liquid Substances 0.000 claims abstract description 25
- 238000000265 homogenisation Methods 0.000 claims abstract description 21
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- 238000010828 elution Methods 0.000 claims abstract description 18
- 239000011780 sodium chloride Substances 0.000 claims abstract description 16
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- 238000004255 ion exchange chromatography Methods 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
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- 239000002253 acid Substances 0.000 claims abstract description 3
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- 229940108924 conjugated linoleic acid Drugs 0.000 description 15
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 6
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 229910021641 deionized water Inorganic materials 0.000 description 3
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- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
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- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
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- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
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- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
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- SYSQUGFVNFXIIT-UHFFFAOYSA-N n-[4-(1,3-benzoxazol-2-yl)phenyl]-4-nitrobenzenesulfonamide Chemical class C1=CC([N+](=O)[O-])=CC=C1S(=O)(=O)NC1=CC=C(C=2OC3=CC=CC=C3N=2)C=C1 SYSQUGFVNFXIIT-UHFFFAOYSA-N 0.000 description 1
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- 230000010355 oscillation Effects 0.000 description 1
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- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
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- 239000002904 solvent Substances 0.000 description 1
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
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CN200710099653XA CN101092613B (zh) | 2007-05-25 | 2007-05-25 | 从亚油酸异构酶产生菌中提取纯化亚油酸异构酶的方法 |
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CN200710099653XA CN101092613B (zh) | 2007-05-25 | 2007-05-25 | 从亚油酸异构酶产生菌中提取纯化亚油酸异构酶的方法 |
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CN102093994A (zh) * | 2010-12-07 | 2011-06-15 | 淮阴工学院 | 一种同时纯化和固定化亚油酸异构酶的方法 |
CN107417765B (zh) * | 2017-09-26 | 2020-12-04 | 珠海联邦制药股份有限公司 | 大肠杆菌自溶表达体系中分离纯化重组蛋白的方法 |
CN112708587B (zh) * | 2019-10-25 | 2022-08-23 | 江南大学 | 一种可高产共轭亚油酸的植物乳杆菌工程菌 |
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Non-Patent Citations (6)
Title |
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曹健等.一株嗜酸乳杆菌突变株亚油酸异构酶的纯化及性质.食品与发酵工业30 2.2004,30(2),48-52. |
曹健等.一株嗜酸乳杆菌突变株亚油酸异构酶的纯化及性质.食品与发酵工业30 2.2004,30(2),48-52. * |
苗士达.植物乳杆菌亚油酸异构酶的分离纯化及其性质研究.食品与发酵工业31 3.2005,31(3),12-15. |
苗士达.植物乳杆菌亚油酸异构酶的分离纯化及其性质研究.食品与发酵工业31 3.2005,31(3),12-15. * |
苗士达.植物乳杆菌亚油酸异构酶的纯化及其应用研究.中国优秀博硕士学位论文全文数据库(硕士)工程科技Ⅰ辑 3.2005,(3),B024-6. |
苗士达.植物乳杆菌亚油酸异构酶的纯化及其应用研究.中国优秀博硕士学位论文全文数据库(硕士)工程科技Ⅰ辑 3.2005,(3),B024-6. * |
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Assignee: Jiangsu Junlebao Dairy Co.,Ltd. Assignor: Institute of Agricultural Product Processing, Chinese Academy of Agricultural Science Contract record no.: 2012320000412 Denomination of invention: Method for extracting pyrified isomerase of linoleic acid from strain of producing isomerase of linoleic acid Granted publication date: 20100623 License type: Exclusive License Open date: 20071226 Record date: 20120406 |
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Effective date of registration: 20170724 Address after: 238000 Anhui province Hefei Jinchao Chaohu Road Economic Development Zone No. 1, North building two Patentee after: Hefei agricultural food science and nutrition Innovation Research Institute Co. Ltd. Address before: 100094 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: Institute of Agricultural Product Processing, Chinese Academy of Agricultural Science |