CN101058830A - 猪瘟病毒荧光定量rt-pcr诊断试剂盒 - Google Patents
猪瘟病毒荧光定量rt-pcr诊断试剂盒 Download PDFInfo
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- CN101058830A CN101058830A CN 200610109404 CN200610109404A CN101058830A CN 101058830 A CN101058830 A CN 101058830A CN 200610109404 CN200610109404 CN 200610109404 CN 200610109404 A CN200610109404 A CN 200610109404A CN 101058830 A CN101058830 A CN 101058830A
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Description
CT值 | CSFV模板 | |||||
HCLV | SM | GDZ1/95 | HeBHH1/95 | BJCY1/96 | JL1/94 | |
T1(J1、J2)T2(J3、J4)T3(J5、J6)T4(J7、J8)T5(J9、J10) | 17.3028.2121.1722.4527.89 | 11.4127.1518.4322.1425.38 | 7.5617.3211.2613.4614.76 | 23.65--29.8029.86-- | 6.6817.927.8615.2117.80 | 7.3719.569.9215.1418.22 |
CT值 | 模板稀释度 | |||||||||
100 | 10-1 | 10-2 | 10-3 | 10-4 | 10i-5 | 10-6 | 10-7 | 10-8 | 10-9 | |
荧光RT-PCRNest-PCR | 6.16+ | 9.47+ | 12.87+ | 15.90+ | 18.63+ | 21.98+ | 25.19- | 28.76- | 29.95- | 29.86- |
试剂 | 用量 |
RT-PCR反应液 | 11.0μl |
反应酶 | 1.3μl |
探针溶液 | 1.5μl |
Claims (1)
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CNB200610109404XA CN100451129C (zh) | 2006-08-11 | 2006-08-11 | 猪瘟病毒荧光定量rt-pcr诊断试剂盒 |
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CNB200610109404XA CN100451129C (zh) | 2006-08-11 | 2006-08-11 | 猪瘟病毒荧光定量rt-pcr诊断试剂盒 |
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CN101058830A true CN101058830A (zh) | 2007-10-24 |
CN100451129C CN100451129C (zh) | 2009-01-14 |
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Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101328506B (zh) * | 2008-07-24 | 2010-11-03 | 湖北省农业科学院畜牧兽医研究所 | 一种特异检测猪瘟病毒野毒感染的荧光定量pcr快速诊断试剂盒及其应用方法 |
CN101463396B (zh) * | 2009-01-06 | 2011-06-29 | 天津出入境检验检疫局动植物与食品检测中心 | 非洲猪瘟病毒荧光定量pcr检测试剂及其制备方法和用途 |
CN102212617A (zh) * | 2011-05-16 | 2011-10-12 | 北京世纪元亨动物防疫技术有限公司 | 用于检测猪瘟病毒野毒株的引物对、探针、以及试剂盒 |
CN101348837B (zh) * | 2008-07-18 | 2012-03-14 | 黄萍 | 用于猪瘟病毒实时荧光定量rt-pcr检测的探针、引物及试剂盒 |
CN101812539B (zh) * | 2009-12-03 | 2012-07-04 | 中国兽医药品监察所 | 猪瘟病毒TaqMan-MGB荧光定量RT-PCR鉴别检测试剂盒及其生产方法 |
CN102676690A (zh) * | 2011-10-31 | 2012-09-19 | 武汉大学 | 猪瘟病毒核酸定量检测试剂盒、检测方法、引物及其探针 |
CN102816864A (zh) * | 2011-06-08 | 2012-12-12 | 山东省滨州畜牧兽医研究院 | 一种检测猪瘟病毒的诊断方法和专用试剂盒 |
CN103436633A (zh) * | 2013-06-29 | 2013-12-11 | 中国农业科学院兰州兽医研究所 | 一种用于检测猪瘟病毒的Taqman Real-time RT-PCR试剂盒 |
CN113999939A (zh) * | 2021-12-30 | 2022-02-01 | 北京纳百生物科技有限公司 | 一种用于猪瘟病毒荧光定量rt-pcr检测的引物及试剂盒 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1164767C (zh) * | 2002-11-08 | 2004-09-01 | 武汉大学 | 一种快速定量检测猪瘟病毒和猪瘟兔化弱毒疫苗的荧光定量pcr试剂盒及应用 |
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2006
- 2006-08-11 CN CNB200610109404XA patent/CN100451129C/zh not_active Expired - Fee Related
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101348837B (zh) * | 2008-07-18 | 2012-03-14 | 黄萍 | 用于猪瘟病毒实时荧光定量rt-pcr检测的探针、引物及试剂盒 |
CN101328506B (zh) * | 2008-07-24 | 2010-11-03 | 湖北省农业科学院畜牧兽医研究所 | 一种特异检测猪瘟病毒野毒感染的荧光定量pcr快速诊断试剂盒及其应用方法 |
CN101463396B (zh) * | 2009-01-06 | 2011-06-29 | 天津出入境检验检疫局动植物与食品检测中心 | 非洲猪瘟病毒荧光定量pcr检测试剂及其制备方法和用途 |
CN101812539B (zh) * | 2009-12-03 | 2012-07-04 | 中国兽医药品监察所 | 猪瘟病毒TaqMan-MGB荧光定量RT-PCR鉴别检测试剂盒及其生产方法 |
CN102212617A (zh) * | 2011-05-16 | 2011-10-12 | 北京世纪元亨动物防疫技术有限公司 | 用于检测猪瘟病毒野毒株的引物对、探针、以及试剂盒 |
CN102212617B (zh) * | 2011-05-16 | 2013-04-10 | 北京世纪元亨动物防疫技术有限公司 | 用于检测猪瘟病毒野毒株的引物对、探针、以及试剂盒 |
CN102816864A (zh) * | 2011-06-08 | 2012-12-12 | 山东省滨州畜牧兽医研究院 | 一种检测猪瘟病毒的诊断方法和专用试剂盒 |
CN102676690A (zh) * | 2011-10-31 | 2012-09-19 | 武汉大学 | 猪瘟病毒核酸定量检测试剂盒、检测方法、引物及其探针 |
CN102676690B (zh) * | 2011-10-31 | 2013-12-18 | 武汉大学 | 猪瘟病毒核酸定量检测试剂盒、检测方法、引物及其探针 |
CN103436633A (zh) * | 2013-06-29 | 2013-12-11 | 中国农业科学院兰州兽医研究所 | 一种用于检测猪瘟病毒的Taqman Real-time RT-PCR试剂盒 |
CN113999939A (zh) * | 2021-12-30 | 2022-02-01 | 北京纳百生物科技有限公司 | 一种用于猪瘟病毒荧光定量rt-pcr检测的引物及试剂盒 |
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CN100451129C (zh) | 2009-01-14 |
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