CN100381576C - 核酸检测方法 - Google Patents

核酸检测方法 Download PDF

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Publication number
CN100381576C
CN100381576C CNB998048712A CN99804871A CN100381576C CN 100381576 C CN100381576 C CN 100381576C CN B998048712 A CNB998048712 A CN B998048712A CN 99804871 A CN99804871 A CN 99804871A CN 100381576 C CN100381576 C CN 100381576C
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CN
China
Prior art keywords
target
nucleic acid
sequence
oligonucleotide probe
oligonucleotide
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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CNB998048712A
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English (en)
Chinese (zh)
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CN1308685A (zh
Inventor
K·鲁迪
K·S·雅各布森
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Genpoint AS
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CNB998048712A 1998-04-01 1999-04-01 核酸检测方法 Expired - Lifetime CN100381576C (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9807045.1A GB9807045D0 (en) 1998-04-01 1998-04-01 Nucleic acid detection method
GB9807045.1 1998-04-01

Publications (2)

Publication Number Publication Date
CN1308685A CN1308685A (zh) 2001-08-15
CN100381576C true CN100381576C (zh) 2008-04-16

Family

ID=10829712

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB998048712A Expired - Lifetime CN100381576C (zh) 1998-04-01 1999-04-01 核酸检测方法

Country Status (11)

Country Link
US (2) US6617138B1 (https=)
EP (1) EP1068352B1 (https=)
JP (1) JP4481491B2 (https=)
CN (1) CN100381576C (https=)
AT (1) ATE377091T1 (https=)
AU (1) AU769566B2 (https=)
CA (1) CA2325052C (https=)
DE (1) DE69937447T2 (https=)
GB (1) GB9807045D0 (https=)
NO (1) NO326359B1 (https=)
WO (1) WO1999050448A2 (https=)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
US7732138B2 (en) * 2001-11-07 2010-06-08 Diagcor Bioscience Incorporation Limited Rapid genotyping analysis and the device thereof
ATE533419T1 (de) * 2003-05-17 2011-12-15 Depuy Int Ltd Marknagel-system
FI20030771A7 (fi) * 2003-05-21 2004-11-22 Helsingin Yliopisto Menetelmä toksisten syanobakteerien määrittämiseksi
US7338763B2 (en) 2004-06-02 2008-03-04 Eppendorf Array Technologies S.A. Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays
US20060057611A1 (en) 2004-06-30 2006-03-16 Applera Corporation Log-linear amplification
FR2873388B1 (fr) * 2004-07-23 2012-04-06 Biomerieux Sa Procede de marquage et de purification d'acides nucleiques d'interet presents dans un echantillon biologique a traiter dans un unique recipient reactionnel
CA2596495A1 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
US7964413B2 (en) 2005-03-10 2011-06-21 Gen-Probe Incorporated Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay
WO2006128230A1 (en) * 2005-05-31 2006-12-07 Newsouth Innovations Pty Limited Detection of hepatotoxic cyanobacteria
EP2334808B1 (en) * 2008-10-01 2017-11-15 Koninklijke Philips N.V. Method for testing and quality controlling of nucleic acids on a support
GB2475226A (en) 2009-11-03 2011-05-18 Genetic Analysis As Universal Prokaryote 16S ribosome PCR primer pair
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
GB201021399D0 (en) 2010-12-16 2011-01-26 Genetic Analysis As Oligonucleotide probe set and methods of microbiota profiling
GB201021397D0 (en) 2010-12-16 2011-01-26 Genetic Analysis As Diagnosis of Crohn's disease
GB201102693D0 (en) 2011-02-16 2011-03-30 Genetic Analysis As Method for identifying neonates at risk for necrotizing enterocolitis
AU2012222178B2 (en) 2011-02-24 2014-12-18 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
GB201110712D0 (en) 2011-06-23 2011-08-10 Univ Ulster Diagnostic methods
DE102012204366B4 (de) * 2011-12-16 2017-07-27 Siemens Aktiengesellschaft Verfahren und Kit zur Identifizierung und Quantifizierung von einer einzelsträngigen Ziel-Nukleinsäure
GB201501603D0 (en) 2015-01-30 2015-03-18 Norwegian University Of Life Sciences And Hedmark University College Method and product
PL3593139T3 (pl) 2017-03-07 2025-11-17 Elypta Ab Biomarkery nowotworowe
CN107287291B (zh) * 2017-06-02 2021-01-05 华南师范大学 一种基于g-C3N4与CdTe/CdS量子点相互作用的双标记核酸检测方法
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
EP3921325A4 (en) * 2019-02-08 2022-11-23 Board of Regents, The University of Texas System ISOLATION AND DETECTION OF AN EXOSOME-ASSOCIATED MICROBIOME FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES
CN110878298B (zh) * 2019-11-22 2023-09-15 深圳市易基因科技有限公司 rRNA捕获探针及其应用
EP4413164A1 (en) 2021-10-04 2024-08-14 Age Labs AS Screening method for rheumatoid arthritis
WO2024008955A1 (en) 2022-07-08 2024-01-11 Age Labs As Method of screening for severe covid-19 susceptibility

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5710028A (en) * 1992-07-02 1998-01-20 Eyal; Nurit Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor
BR9306867A (pt) * 1992-07-17 1998-12-08 Aprogenex Inc Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ
WO1994009156A1 (en) * 1992-10-08 1994-04-28 The Regents Of The University Of California Pcr assays to determine the presence and concentration of a target
WO1997032044A1 (en) * 1996-03-01 1997-09-04 E.I. Du Pont De Nemours And Company A method for the amplification and detection of a nucleic acid fragment of interest
DE19612684A1 (de) * 1996-03-29 1997-10-02 Gsf Forschungszentrum Umwelt Neue Verwendung extrem thermophiler DNA-Polymerasen

Also Published As

Publication number Publication date
EP1068352B1 (en) 2007-10-31
CA2325052C (en) 2011-08-02
WO1999050448A3 (en) 1999-12-02
WO1999050448A2 (en) 1999-10-07
US20040197794A1 (en) 2004-10-07
AU3338999A (en) 1999-10-18
EP1068352A2 (en) 2001-01-17
CA2325052A1 (en) 1999-10-07
NO20004962L (no) 2000-12-01
DE69937447D1 (de) 2007-12-13
AU769566B2 (en) 2004-01-29
DE69937447T2 (de) 2008-08-21
JP4481491B2 (ja) 2010-06-16
CN1308685A (zh) 2001-08-15
JP2003516710A (ja) 2003-05-20
ATE377091T1 (de) 2007-11-15
NO20004962D0 (no) 2000-10-02
US6617138B1 (en) 2003-09-09
NO326359B1 (no) 2008-11-17
GB9807045D0 (en) 1998-06-03

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Effective date of registration: 20090109

Address after: Oslo, Norway

Patentee after: GENETIC ANALYSIS A/S

Address before: Oslo, Norway

Patentee before: Matt fauske Limited by Share Ltd.

Effective date of registration: 20090109

Address after: Oslo, Norway

Patentee after: Matt fauske Limited by Share Ltd.

Address before: Oslo, Norway

Patentee before: GENPOINT A/S

ASS Succession or assignment of patent right

Owner name: MAAT FAUSKE CO., LTD.

Free format text: FORMER OWNER: JIANPOENTE JOINT CO., LTD.

Effective date: 20090109

Owner name: GENETIC ANALYSIS CO., LTD.

Free format text: FORMER OWNER: MAAT FAUSKE CO., LTD.

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Granted publication date: 20080416

CX01 Expiry of patent term