CA2325052C - Nucleic acid detection method - Google Patents
Nucleic acid detection method Download PDFInfo
- Publication number
- CA2325052C CA2325052C CA2325052A CA2325052A CA2325052C CA 2325052 C CA2325052 C CA 2325052C CA 2325052 A CA2325052 A CA 2325052A CA 2325052 A CA2325052 A CA 2325052A CA 2325052 C CA2325052 C CA 2325052C
- Authority
- CA
- Canada
- Prior art keywords
- target
- nucleic acid
- sequence
- labelled
- oligonucleotide probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- 238000000034 method Methods 0.000 claims abstract description 83
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9807045.1A GB9807045D0 (en) | 1998-04-01 | 1998-04-01 | Nucleic acid detection method |
| GB9807045.1 | 1998-04-01 | ||
| PCT/GB1999/001025 WO1999050448A2 (en) | 1998-04-01 | 1999-04-01 | Nucleic acid detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2325052A1 CA2325052A1 (en) | 1999-10-07 |
| CA2325052C true CA2325052C (en) | 2011-08-02 |
Family
ID=10829712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2325052A Expired - Lifetime CA2325052C (en) | 1998-04-01 | 1999-04-01 | Nucleic acid detection method |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US6617138B1 (https=) |
| EP (1) | EP1068352B1 (https=) |
| JP (1) | JP4481491B2 (https=) |
| CN (1) | CN100381576C (https=) |
| AT (1) | ATE377091T1 (https=) |
| AU (1) | AU769566B2 (https=) |
| CA (1) | CA2325052C (https=) |
| DE (1) | DE69937447T2 (https=) |
| GB (1) | GB9807045D0 (https=) |
| NO (1) | NO326359B1 (https=) |
| WO (1) | WO1999050448A2 (https=) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10012540B4 (de) * | 2000-03-15 | 2004-09-23 | Vermicon Ag | Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion |
| US7732138B2 (en) * | 2001-11-07 | 2010-06-08 | Diagcor Bioscience Incorporation Limited | Rapid genotyping analysis and the device thereof |
| ATE533419T1 (de) * | 2003-05-17 | 2011-12-15 | Depuy Int Ltd | Marknagel-system |
| FI20030771A7 (fi) * | 2003-05-21 | 2004-11-22 | Helsingin Yliopisto | Menetelmä toksisten syanobakteerien määrittämiseksi |
| US7338763B2 (en) | 2004-06-02 | 2008-03-04 | Eppendorf Array Technologies S.A. | Method and kit for the detection and/or quantification of homologous nucleotide sequences on arrays |
| US20060057611A1 (en) | 2004-06-30 | 2006-03-16 | Applera Corporation | Log-linear amplification |
| FR2873388B1 (fr) * | 2004-07-23 | 2012-04-06 | Biomerieux Sa | Procede de marquage et de purification d'acides nucleiques d'interet presents dans un echantillon biologique a traiter dans un unique recipient reactionnel |
| CA2596495A1 (en) * | 2005-02-09 | 2006-08-17 | Pacific Biosciences Of California, Inc. | Nucleotide compositions and uses thereof |
| US7964413B2 (en) | 2005-03-10 | 2011-06-21 | Gen-Probe Incorporated | Method for continuous mode processing of multiple reaction receptacles in a real-time amplification assay |
| WO2006128230A1 (en) * | 2005-05-31 | 2006-12-07 | Newsouth Innovations Pty Limited | Detection of hepatotoxic cyanobacteria |
| EP2334808B1 (en) * | 2008-10-01 | 2017-11-15 | Koninklijke Philips N.V. | Method for testing and quality controlling of nucleic acids on a support |
| GB2475226A (en) | 2009-11-03 | 2011-05-18 | Genetic Analysis As | Universal Prokaryote 16S ribosome PCR primer pair |
| US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
| GB201021399D0 (en) | 2010-12-16 | 2011-01-26 | Genetic Analysis As | Oligonucleotide probe set and methods of microbiota profiling |
| GB201021397D0 (en) | 2010-12-16 | 2011-01-26 | Genetic Analysis As | Diagnosis of Crohn's disease |
| GB201102693D0 (en) | 2011-02-16 | 2011-03-30 | Genetic Analysis As | Method for identifying neonates at risk for necrotizing enterocolitis |
| AU2012222178B2 (en) | 2011-02-24 | 2014-12-18 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| GB201110712D0 (en) | 2011-06-23 | 2011-08-10 | Univ Ulster | Diagnostic methods |
| DE102012204366B4 (de) * | 2011-12-16 | 2017-07-27 | Siemens Aktiengesellschaft | Verfahren und Kit zur Identifizierung und Quantifizierung von einer einzelsträngigen Ziel-Nukleinsäure |
| GB201501603D0 (en) | 2015-01-30 | 2015-03-18 | Norwegian University Of Life Sciences And Hedmark University College | Method and product |
| PL3593139T3 (pl) | 2017-03-07 | 2025-11-17 | Elypta Ab | Biomarkery nowotworowe |
| CN107287291B (zh) * | 2017-06-02 | 2021-01-05 | 华南师范大学 | 一种基于g-C3N4与CdTe/CdS量子点相互作用的双标记核酸检测方法 |
| WO2019200326A1 (en) | 2018-04-13 | 2019-10-17 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| EP3921325A4 (en) * | 2019-02-08 | 2022-11-23 | Board of Regents, The University of Texas System | ISOLATION AND DETECTION OF AN EXOSOME-ASSOCIATED MICROBIOME FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES |
| CN110878298B (zh) * | 2019-11-22 | 2023-09-15 | 深圳市易基因科技有限公司 | rRNA捕获探针及其应用 |
| EP4413164A1 (en) | 2021-10-04 | 2024-08-14 | Age Labs AS | Screening method for rheumatoid arthritis |
| WO2024008955A1 (en) | 2022-07-08 | 2024-01-11 | Age Labs As | Method of screening for severe covid-19 susceptibility |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5710028A (en) * | 1992-07-02 | 1998-01-20 | Eyal; Nurit | Method of quick screening and identification of specific DNA sequences by single nucleotide primer extension and kits therefor |
| BR9306867A (pt) * | 1992-07-17 | 1998-12-08 | Aprogenex Inc | Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ |
| WO1994009156A1 (en) * | 1992-10-08 | 1994-04-28 | The Regents Of The University Of California | Pcr assays to determine the presence and concentration of a target |
| WO1997032044A1 (en) * | 1996-03-01 | 1997-09-04 | E.I. Du Pont De Nemours And Company | A method for the amplification and detection of a nucleic acid fragment of interest |
| DE19612684A1 (de) * | 1996-03-29 | 1997-10-02 | Gsf Forschungszentrum Umwelt | Neue Verwendung extrem thermophiler DNA-Polymerasen |
-
1998
- 1998-04-01 GB GBGB9807045.1A patent/GB9807045D0/en not_active Ceased
-
1999
- 1999-04-01 US US09/646,847 patent/US6617138B1/en not_active Expired - Lifetime
- 1999-04-01 AU AU33389/99A patent/AU769566B2/en not_active Ceased
- 1999-04-01 WO PCT/GB1999/001025 patent/WO1999050448A2/en not_active Ceased
- 1999-04-01 CN CNB998048712A patent/CN100381576C/zh not_active Expired - Lifetime
- 1999-04-01 EP EP99914659A patent/EP1068352B1/en not_active Expired - Lifetime
- 1999-04-01 JP JP2000541335A patent/JP4481491B2/ja not_active Expired - Lifetime
- 1999-04-01 DE DE69937447T patent/DE69937447T2/de not_active Expired - Lifetime
- 1999-04-01 CA CA2325052A patent/CA2325052C/en not_active Expired - Lifetime
- 1999-04-01 AT AT99914659T patent/ATE377091T1/de not_active IP Right Cessation
-
2000
- 2000-10-02 NO NO20004962A patent/NO326359B1/no not_active IP Right Cessation
-
2003
- 2003-09-09 US US10/658,100 patent/US20040197794A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1068352B1 (en) | 2007-10-31 |
| WO1999050448A3 (en) | 1999-12-02 |
| WO1999050448A2 (en) | 1999-10-07 |
| US20040197794A1 (en) | 2004-10-07 |
| AU3338999A (en) | 1999-10-18 |
| EP1068352A2 (en) | 2001-01-17 |
| CA2325052A1 (en) | 1999-10-07 |
| NO20004962L (no) | 2000-12-01 |
| DE69937447D1 (de) | 2007-12-13 |
| AU769566B2 (en) | 2004-01-29 |
| DE69937447T2 (de) | 2008-08-21 |
| JP4481491B2 (ja) | 2010-06-16 |
| CN1308685A (zh) | 2001-08-15 |
| CN100381576C (zh) | 2008-04-16 |
| JP2003516710A (ja) | 2003-05-20 |
| ATE377091T1 (de) | 2007-11-15 |
| NO20004962D0 (no) | 2000-10-02 |
| US6617138B1 (en) | 2003-09-09 |
| NO326359B1 (no) | 2008-11-17 |
| GB9807045D0 (en) | 1998-06-03 |
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