CL2022003128A1 - Método para obtener plantas mutantes mediante mutagénesis dirigida - Google Patents

Método para obtener plantas mutantes mediante mutagénesis dirigida

Info

Publication number
CL2022003128A1
CL2022003128A1 CL2022003128A CL2022003128A CL2022003128A1 CL 2022003128 A1 CL2022003128 A1 CL 2022003128A1 CL 2022003128 A CL2022003128 A CL 2022003128A CL 2022003128 A CL2022003128 A CL 2022003128A CL 2022003128 A1 CL2022003128 A1 CL 2022003128A1
Authority
CL
Chile
Prior art keywords
plants
directed mutagenesis
mutant plants
rna
exogenous dna
Prior art date
Application number
CL2022003128A
Other languages
English (en)
Inventor
Vriezen Wim
Original Assignee
Nunhems Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nunhems Bv filed Critical Nunhems Bv
Publication of CL2022003128A1 publication Critical patent/CL2022003128A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para la introducción y selección de una mutación hereditaria específica en una planta, que comprende transfectar células vegetales con ADN exógeno, en donde dicho ADN exógeno codifica una ADN endonucleasa guiada por ARN, ARN guía adecuado para dirigir la ADN endonucleasa guiada por ARN para inducir dicha mutación hereditaria específica y un marcador de selección; regenerar plantas a partir de dichas células transfectadas para proporcionar una pluralidad de plantas T0; cruzar las plantas T0 con plantas isogénicas que no comprenden dicho ADN exógeno para proporcionar una pluralidad de plantas de progenie; y seleccionar una o más plantas que tienen la mutación hereditaria de las plantas de progenie.
CL2022003128A 2020-05-13 2022-11-10 Método para obtener plantas mutantes mediante mutagénesis dirigida CL2022003128A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP20174401 2020-05-13

Publications (1)

Publication Number Publication Date
CL2022003128A1 true CL2022003128A1 (es) 2023-05-12

Family

ID=70682770

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2022003128A CL2022003128A1 (es) 2020-05-13 2022-11-10 Método para obtener plantas mutantes mediante mutagénesis dirigida

Country Status (11)

Country Link
US (1) US20230183725A1 (es)
EP (1) EP4149243A1 (es)
JP (1) JP2023526035A (es)
KR (1) KR20230010678A (es)
CN (1) CN115697043A (es)
AU (1) AU2021271086A1 (es)
CA (1) CA3178193A1 (es)
CL (1) CL2022003128A1 (es)
IL (1) IL298021A (es)
MX (1) MX2022014186A (es)
WO (1) WO2021228700A1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105264067B (zh) * 2012-09-07 2020-11-10 美国陶氏益农公司 Fad3性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白
LT3036327T (lt) * 2013-08-22 2019-06-25 Pioneer Hi-Bred International, Inc. Genomo modifikacijos, panaudojant nukreipiančias polinukleotido/cas endonukleazės sistemas, ir panaudojimo būdai
WO2016007347A1 (en) * 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2016341041A1 (en) * 2015-10-20 2018-03-15 Pioneer Hi-Bred International, Inc. Methods and compositions for marker-free genome modification
CN108085328B (zh) * 2016-11-21 2021-06-22 中国科学院分子植物科学卓越创新中心 一种dna序列编辑的方法

Also Published As

Publication number Publication date
MX2022014186A (es) 2022-12-07
KR20230010678A (ko) 2023-01-19
WO2021228700A1 (en) 2021-11-18
IL298021A (en) 2023-01-01
CA3178193A1 (en) 2021-11-18
EP4149243A1 (en) 2023-03-22
US20230183725A1 (en) 2023-06-15
JP2023526035A (ja) 2023-06-20
CN115697043A (zh) 2023-02-03
AU2021271086A1 (en) 2022-12-15

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