MX2022014186A - Metodo para obtener plantas mutantes mediante mutagenesis dirigida. - Google Patents

Metodo para obtener plantas mutantes mediante mutagenesis dirigida.

Info

Publication number
MX2022014186A
MX2022014186A MX2022014186A MX2022014186A MX2022014186A MX 2022014186 A MX2022014186 A MX 2022014186A MX 2022014186 A MX2022014186 A MX 2022014186A MX 2022014186 A MX2022014186 A MX 2022014186A MX 2022014186 A MX2022014186 A MX 2022014186A
Authority
MX
Mexico
Prior art keywords
plants
mutant plants
targeted mutagenesis
rna
exogenous dna
Prior art date
Application number
MX2022014186A
Other languages
English (en)
Inventor
Wim Vriezen
Original Assignee
Nunhems Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nunhems Bv filed Critical Nunhems Bv
Publication of MX2022014186A publication Critical patent/MX2022014186A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A01H1/021—Methods of breeding using interspecific crosses, i.e. interspecies crosses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a un método para la introducción y selección de una mutación hereditaria específica en una planta, que comprende transfectar células vegetales con ADN exógeno, en donde dicho ADN exógeno codifica una ADN endonucleasa guiada por ARN, ARN guía adecuado para dirigir la ADN endonucleasa guiada por ARN para inducir dicha mutación hereditaria específica y un marcador de selección; regenerar plantas a partir de dichas células transfectadas para proporcionar una pluralidad de plantas T0; cruzar las plantas T0 con plantas isogénicas que no comprenden dicho ADN exógeno para proporcionar una pluralidad de plantas de progenie; y seleccionar una o más plantas que tienen la mutación hereditaria de las plantas de progenie.
MX2022014186A 2020-05-13 2021-05-07 Metodo para obtener plantas mutantes mediante mutagenesis dirigida. MX2022014186A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20174401 2020-05-13
PCT/EP2021/062116 WO2021228700A1 (en) 2020-05-13 2021-05-07 Method for obtaining mutant plants by targeted mutagenesis

Publications (1)

Publication Number Publication Date
MX2022014186A true MX2022014186A (es) 2022-12-07

Family

ID=70682770

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022014186A MX2022014186A (es) 2020-05-13 2021-05-07 Metodo para obtener plantas mutantes mediante mutagenesis dirigida.

Country Status (11)

Country Link
US (1) US20230183725A1 (es)
EP (1) EP4149243A1 (es)
JP (1) JP2023526035A (es)
KR (1) KR20230010678A (es)
CN (1) CN115697043A (es)
AU (1) AU2021271086A1 (es)
CA (1) CA3178193A1 (es)
CL (1) CL2022003128A1 (es)
IL (1) IL298021A (es)
MX (1) MX2022014186A (es)
WO (1) WO2021228700A1 (es)

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2884162C (en) * 2012-09-07 2020-12-29 Dow Agrosciences Llc Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
US20160201072A1 (en) * 2013-08-22 2016-07-14 Pioneer Hi-Bred International, Inc. Genome modification using guide polynucleotide/cas endonuclease systems and methods of use
BR112017000482A2 (pt) * 2014-07-11 2017-11-07 Du Pont métodos para produzir uma planta mutante e para gerar uma planta, planta, semente, rna, métodos para produzir uma célula, para duplicar um fragmento gênico, para substituir uma primeira sequência promotora, para inserir um elemento regulador em uma sequência de nucleotídeos e para inserir um íntron em uma sequência de nucleotídeos, planta de milho e célula vegetal
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
MX2018004809A (es) * 2015-10-20 2018-06-08 Pionner Hi Bred Int Inc Metodos y composiciones para la modificacion genomica exenta de marcadores.
CN108085328B (zh) * 2016-11-21 2021-06-22 中国科学院分子植物科学卓越创新中心 一种dna序列编辑的方法

Also Published As

Publication number Publication date
US20230183725A1 (en) 2023-06-15
EP4149243A1 (en) 2023-03-22
CA3178193A1 (en) 2021-11-18
IL298021A (en) 2023-01-01
JP2023526035A (ja) 2023-06-20
KR20230010678A (ko) 2023-01-19
CL2022003128A1 (es) 2023-05-12
WO2021228700A1 (en) 2021-11-18
AU2021271086A1 (en) 2022-12-15
CN115697043A (zh) 2023-02-03

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