CA3202264A1 - Osteoblasts differentiated from mesenchymal stem cells and composition for treating bone disease comprising the same - Google Patents

Osteoblasts differentiated from mesenchymal stem cells and composition for treating bone disease comprising the same Download PDF

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Publication number
CA3202264A1
CA3202264A1 CA3202264A CA3202264A CA3202264A1 CA 3202264 A1 CA3202264 A1 CA 3202264A1 CA 3202264 A CA3202264 A CA 3202264A CA 3202264 A CA3202264 A CA 3202264A CA 3202264 A1 CA3202264 A1 CA 3202264A1
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Prior art keywords
osteoblasts
stem cells
cells
bone
differentiated
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Pending
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CA3202264A
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English (en)
French (fr)
Inventor
Hyun Sook Park
Yeon Kyung Lee
Eun Jin GO
Ha Jin Kim
Sun Ray Lee
Ah Reum KWON
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Cefo Co Ltd
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Cefo Co Ltd
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Publication of CA3202264A1 publication Critical patent/CA3202264A1/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0652Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/32Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00Drugs for skeletal disorders
    • A61P19/08Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/20Material Coatings
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02Membranes; Filters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068General culture methods using substrates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/165Vascular endothelial growth factor [VEGF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells
    • C12N2506/1369Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells from blood-borne mesenchymal stem cells, e.g. MSC from umbilical blood
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/003D culture
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00Supports or coatings for cell culture, characterised by material
    • C12N2533/30Synthetic polymers

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Rheumatology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Epidemiology (AREA)
  • Virology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3202264A 2020-10-08 2021-10-08 Osteoblasts differentiated from mesenchymal stem cells and composition for treating bone disease comprising the same Pending CA3202264A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20200130138 2020-10-08
KR10-2020-0130138 2020-10-08
PCT/KR2021/013900 WO2022075809A1 (ko) 2020-10-08 2021-10-08 중간엽 줄기세포에서 분화된 골모세포 및 이를 포함하는 골질환 치료용 조성물

Publications (1)

Publication Number Publication Date
CA3202264A1 true CA3202264A1 (en) 2022-04-14

Family

ID=81126308

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3202264A Pending CA3202264A1 (en) 2020-10-08 2021-10-08 Osteoblasts differentiated from mesenchymal stem cells and composition for treating bone disease comprising the same

Country Status (7)

Country Link
US (1) US20230248776A1 (ko)
EP (1) EP4227404A1 (ko)
JP (1) JP2023521097A (ko)
KR (1) KR20220047199A (ko)
CN (1) CN115516081A (ko)
CA (1) CA3202264A1 (ko)
WO (1) WO2022075809A1 (ko)

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008297220A (ja) * 2007-05-29 2008-12-11 Metabolome Pharmaceuticals Inc 骨折および骨量減少を伴う骨疾患の予防および治療剤
US8580757B2 (en) 2007-08-09 2013-11-12 Thermo Fisher Scientific Biosciences Inc. Methods of modulating mesenchymal stem cell differentiation
KR100947821B1 (ko) * 2008-01-09 2010-03-15 차의과학대학교 산학협력단 중간엽 줄기 세포의 골아세포로의 분화방법 및 이를 위한분화용 배지
JP2009256350A (ja) * 2008-03-28 2009-11-05 Institute Of Physical & Chemical Research 骨分化誘導剤
ES2370205B2 (es) * 2010-05-18 2012-07-04 Universidad De Santiago De Compostela Uso de poloxaminas como inductoras de la diferenciacion osteogenica de celulas mesenquimales
KR101367896B1 (ko) * 2012-01-30 2014-02-26 건국대학교 산학협력단 Msm을 유효성분으로 포함하는 뼈 성장 활성용 조성물
US20140206022A1 (en) * 2012-12-17 2014-07-24 Allergan, Inc. Three-dimensional cell culture methods for test material assessment of cell differentiation
KR101860301B1 (ko) 2014-09-19 2018-05-24 (주)세포바이오 하이드로겔을 이용한 3차원적 줄기세포 골분화 유도 방법
KR101773343B1 (ko) * 2015-10-16 2017-08-31 원광대학교산학협력단 강글리오사이드 gd3을 유효성분으로 함유하는 중간엽 줄기세포로부터 조골세포 분화 유도용 조성물
KR102061740B1 (ko) * 2017-01-25 2020-01-02 (주)세포바이오 골재생용 세포 치료제 및 이것의 제조 방법
KR20180114307A (ko) 2017-04-10 2018-10-18 주식회사 원앤드 기능성 화합물을 함유하는 하이드로젤 화장료 조성물
AU2019345976A1 (en) * 2018-09-25 2021-05-20 Bone Therapeutics Sa Methods for differentiating mesenchymal stem cells
CN110577929A (zh) * 2019-09-18 2019-12-17 安徽科门生物科技有限公司 一种脐带间充质干细胞的分离培养方法

Also Published As

Publication number Publication date
JP2023521097A (ja) 2023-05-23
US20230248776A1 (en) 2023-08-10
CN115516081A (zh) 2022-12-23
KR20220047199A (ko) 2022-04-15
WO2022075809A1 (ko) 2022-04-14
EP4227404A1 (en) 2023-08-16

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