CA3158318A1 - Virtual fiducials - Google Patents
Virtual fiducialsInfo
- Publication number
- CA3158318A1 CA3158318A1 CA3158318A CA3158318A CA3158318A1 CA 3158318 A1 CA3158318 A1 CA 3158318A1 CA 3158318 A CA3158318 A CA 3158318A CA 3158318 A CA3158318 A CA 3158318A CA 3158318 A1 CA3158318 A1 CA 3158318A1
- Authority
- CA
- Canada
- Prior art keywords
- detector
- fiducial
- lens
- objective lens
- microscope
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000003287 optical effect Effects 0.000 claims abstract description 98
- 238000003384 imaging method Methods 0.000 claims abstract description 28
- 230000008859 change Effects 0.000 claims abstract description 9
- 239000007850 fluorescent dye Substances 0.000 claims description 36
- 238000000034 method Methods 0.000 claims description 18
- 239000012530 fluid Substances 0.000 claims description 4
- 230000003213 activating effect Effects 0.000 claims 1
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 239000011521 glass Substances 0.000 description 17
- 238000005286 illumination Methods 0.000 description 13
- 239000002773 nucleotide Substances 0.000 description 10
- 125000003729 nucleotide group Chemical group 0.000 description 10
- 238000000926 separation method Methods 0.000 description 10
- 230000004044 response Effects 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 7
- 102000039446 nucleic acids Human genes 0.000 description 7
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- 239000007787 solid Substances 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 5
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- 238000000576 coating method Methods 0.000 description 4
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- 239000011248 coating agent Substances 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- RYVNIFSIEDRLSJ-UHFFFAOYSA-N 5-(hydroxymethyl)cytosine Chemical compound NC=1NC(=O)N=CC=1CO RYVNIFSIEDRLSJ-UHFFFAOYSA-N 0.000 description 2
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
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- 239000011230 binding agent Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 238000001215 fluorescent labelling Methods 0.000 description 2
- UYTPUPDQBNUYGX-UHFFFAOYSA-N guanine Chemical compound O=C1NC(N)=NC2=C1N=CN2 UYTPUPDQBNUYGX-UHFFFAOYSA-N 0.000 description 2
- DRAVOWXCEBXPTN-UHFFFAOYSA-N isoguanine Chemical compound NC1=NC(=O)NC2=C1NC=N2 DRAVOWXCEBXPTN-UHFFFAOYSA-N 0.000 description 2
- 230000033001 locomotion Effects 0.000 description 2
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical compound CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 description 2
- XQCZBXHVTFVIFE-UHFFFAOYSA-N 2-amino-4-hydroxypyrimidine Chemical compound NC1=NC=CC(O)=N1 XQCZBXHVTFVIFE-UHFFFAOYSA-N 0.000 description 1
- FHSISDGOVSHJRW-UHFFFAOYSA-N 5-formylcytosine Chemical compound NC1=NC(=O)NC=C1C=O FHSISDGOVSHJRW-UHFFFAOYSA-N 0.000 description 1
- LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
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- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
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- 230000005540 biological transmission Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000002041 carbon nanotube Substances 0.000 description 1
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- 230000009087 cell motility Effects 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
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- 230000001419 dependent effect Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
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- 239000002184 metal Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
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- 239000004065 semiconductor Substances 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- 230000000007 visual effect Effects 0.000 description 1
- 239000011800 void material Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/245—Devices for focusing using auxiliary sources, detectors
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/006—Optical details of the image generation focusing arrangements; selection of the plane to be imaged
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/32—Fiducial marks and measuring scales within the optical system
- G02B27/34—Fiducial marks and measuring scales within the optical system illuminated
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US16/658,052 US10895727B1 (en) | 2019-10-19 | 2019-10-19 | Microscope for locating structures on the inner surface of a fluidic channel |
| US16/658,052 | 2019-10-19 | ||
| US202016824632A | 2020-03-19 | 2020-03-19 | |
| US16/824,632 | 2020-03-19 | ||
| PCT/US2020/056302 WO2021077075A1 (en) | 2019-10-19 | 2020-10-19 | Virtual fiducials |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3158318A1 true CA3158318A1 (en) | 2021-04-20 |
Family
ID=75538690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3158318A Pending CA3158318A1 (en) | 2019-10-19 | 2020-10-19 | Virtual fiducials |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP4031922A4 (ja) |
| JP (1) | JP2022552743A (ja) |
| KR (1) | KR20220084147A (ja) |
| CN (1) | CN114585958B (ja) |
| AU (1) | AU2020366521B2 (ja) |
| CA (1) | CA3158318A1 (ja) |
| WO (1) | WO2021077075A1 (ja) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114280806B (zh) * | 2022-02-10 | 2024-08-13 | 中国人民解放军陆军装甲兵学院 | 一种基于相息图的光线准直方法 |
Family Cites Families (34)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3265668B2 (ja) * | 1993-01-13 | 2002-03-11 | 株式会社ニコン | ベストフォーカス位置の算出方法 |
| JP3619571B2 (ja) * | 1995-06-06 | 2005-02-09 | オリンパス株式会社 | 光学顕微鏡における焦点検出装置及びその設計方法 |
| JPH09133856A (ja) * | 1995-11-07 | 1997-05-20 | Nikon Corp | 顕微鏡用自動焦点検出装置 |
| US6025905A (en) * | 1996-12-31 | 2000-02-15 | Cognex Corporation | System for obtaining a uniform illumination reflectance image during periodic structured illumination |
| US6181474B1 (en) * | 1999-03-22 | 2001-01-30 | Kovex Corporation | Scanning confocal microscope with objective lens position tracking |
| US6974938B1 (en) * | 2000-03-08 | 2005-12-13 | Tibotec Bvba | Microscope having a stable autofocusing apparatus |
| JP2002277736A (ja) * | 2001-03-21 | 2002-09-25 | Olympus Optical Co Ltd | 撮像装置 |
| JP3867143B2 (ja) * | 2003-06-25 | 2007-01-10 | 独立行政法人産業技術総合研究所 | 三次元顕微鏡システムおよび画像表示方法 |
| JP2005134834A (ja) * | 2003-10-31 | 2005-05-26 | Olympus Corp | 撮像装置および電子的撮像機器 |
| US7057826B2 (en) * | 2004-03-22 | 2006-06-06 | Angstrom Inc. | Small and fast zoom system |
| US7295303B1 (en) * | 2004-03-25 | 2007-11-13 | Kla-Tencor Technologies Corporation | Methods and apparatus for inspecting a sample |
| JP2006047922A (ja) * | 2004-08-09 | 2006-02-16 | Nikon Corp | 結像装置 |
| JP2006084794A (ja) * | 2004-09-16 | 2006-03-30 | Olympus Corp | 焦点位置制御機構付き観察装置 |
| EP2453223B1 (en) * | 2005-04-12 | 2019-07-03 | Caliper Life Sciences Inc. | Optical detection system for a microfluidic device and method for aligning and focusing an optical detection system |
| JP5058483B2 (ja) * | 2005-09-14 | 2012-10-24 | オリンパス株式会社 | 生体試料の長期間ないし連続的検出方法 |
| JP2009116271A (ja) * | 2007-11-09 | 2009-05-28 | Nikon Corp | 焦点調節装置及び顕微鏡装置 |
| US7723657B2 (en) * | 2007-11-16 | 2010-05-25 | Mitutoyo Corporation | Focus detection apparatus having extended detection range |
| US9213176B2 (en) * | 2008-12-02 | 2015-12-15 | The Regents Of The University Of California | Imaging arrangement and microscope |
| JP5739351B2 (ja) * | 2009-03-11 | 2015-06-24 | サクラ ファインテック ユー．エス．エー．， インコーポレイテッド | 自動合焦方法および自動合焦装置 |
| TWI456254B (zh) * | 2010-05-19 | 2014-10-11 | Ind Tech Res Inst | 螢光顯微影像系統 |
| WO2013027459A1 (ja) * | 2011-08-24 | 2013-02-28 | オリンパスメディカルシステムズ株式会社 | 撮像装置及び撮像装置システム |
| DE102011082756A1 (de) * | 2011-09-15 | 2013-03-21 | Leica Microsystems (Schweiz) Ag | Autofokussierverfahren und -einrichtung für ein Mikroskop |
| DE102012009836A1 (de) * | 2012-05-16 | 2013-11-21 | Carl Zeiss Microscopy Gmbh | Lichtmikroskop und Verfahren zur Bildaufnahme mit einem Lichtmikroskop |
| AU2013295679A1 (en) * | 2012-07-25 | 2015-01-29 | Theranos, Inc. | Image analysis and measurement of biological samples |
| EP2971011B1 (en) * | 2013-03-15 | 2021-11-10 | The Regents of the University of California | High-throughput cargo delivery into live cells using photothermal platforms |
| JP6286183B2 (ja) * | 2013-11-07 | 2018-02-28 | 株式会社日立ハイテクノロジーズ | 分析装置 |
| JP6197880B2 (ja) * | 2013-12-12 | 2017-09-20 | 株式会社ニコン | 構造化照明顕微鏡、構造化照明方法、及びプログラム |
| US9897791B2 (en) | 2014-10-16 | 2018-02-20 | Illumina, Inc. | Optical scanning systems for in situ genetic analysis |
| CA2991920C (en) * | 2015-04-23 | 2024-05-14 | The University Of British Columbia | Multifocal method and apparatus for stabilization of optical systems |
| DE102015209402A1 (de) * | 2015-05-22 | 2016-11-24 | Sirona Dental Systems Gmbh | Vorrichtung zur optischen 3D-Vermessung eines Objekts |
| US10558029B2 (en) * | 2016-10-27 | 2020-02-11 | Scopio Labs Ltd. | System for image reconstruction using a known pattern |
| EP3574307A4 (en) * | 2017-01-26 | 2020-10-21 | Azure Biosystems, Inc. | BIOMOLECULES IMAGING DEVICES AND METHODS |
| JP2019078866A (ja) * | 2017-10-24 | 2019-05-23 | オリンパス株式会社 | 顕微鏡システム、観察方法、及び観察プログラム |
| US10895727B1 (en) * | 2019-10-19 | 2021-01-19 | SequLITE Genomics US, Inc. | Microscope for locating structures on the inner surface of a fluidic channel |
-
2020
- 2020-10-19 CN CN202080073343.4A patent/CN114585958B/zh active Active
- 2020-10-19 EP EP20876361.5A patent/EP4031922A4/en active Pending
- 2020-10-19 AU AU2020366521A patent/AU2020366521B2/en active Active
- 2020-10-19 JP JP2022523406A patent/JP2022552743A/ja active Pending
- 2020-10-19 CA CA3158318A patent/CA3158318A1/en active Pending
- 2020-10-19 KR KR1020227016636A patent/KR20220084147A/ko not_active Application Discontinuation
- 2020-10-19 WO PCT/US2020/056302 patent/WO2021077075A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2022552743A (ja) | 2022-12-19 |
| AU2020366521B2 (en) | 2024-03-07 |
| KR20220084147A (ko) | 2022-06-21 |
| AU2020366521A1 (en) | 2022-05-19 |
| EP4031922A1 (en) | 2022-07-27 |
| EP4031922A4 (en) | 2022-12-07 |
| CN114585958B (zh) | 2024-05-28 |
| CN114585958A (zh) | 2022-06-03 |
| WO2021077075A1 (en) | 2021-04-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |
|
| EEER | Examination request |
Effective date: 20220419 |