CA3149413C - Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kits - Google Patents
Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kitsInfo
- Publication number
- CA3149413C CA3149413C CA3149413A CA3149413A CA3149413C CA 3149413 C CA3149413 C CA 3149413C CA 3149413 A CA3149413 A CA 3149413A CA 3149413 A CA3149413 A CA 3149413A CA 3149413 C CA3149413 C CA 3149413C
- Authority
- CA
- Canada
- Prior art keywords
- cells
- gfp
- ptd4
- tat
- nls
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3149413A CA3149413C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kits |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562145760P | 2015-04-10 | 2015-04-10 | |
| US62/145,760 | 2015-04-10 | ||
| US201562246892P | 2015-10-27 | 2015-10-27 | |
| US62/246,892 | 2015-10-27 | ||
| CA2981716A CA2981716C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
| CA3149413A CA3149413C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kits |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2981716A Division CA2981716C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3149413A1 CA3149413A1 (en) | 2016-10-13 |
| CA3149413C true CA3149413C (en) | 2025-07-08 |
Family
ID=57071637
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2981716A Active CA2981716C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
| CA3149413A Active CA3149413C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based carrier agents to improve the transduction efficiency of polypeptide cargo to cytosol in target eukaryotic cells and their uses, methods, and related kits |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2981716A Active CA2981716C (en) | 2015-04-10 | 2016-04-08 | Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US9738687B2 (enExample) |
| EP (1) | EP3280739B1 (enExample) |
| JP (2) | JP6956705B2 (enExample) |
| KR (2) | KR102697262B1 (enExample) |
| CN (1) | CN107636017B (enExample) |
| AU (1) | AU2016245347B2 (enExample) |
| CA (2) | CA2981716C (enExample) |
| ES (1) | ES2864758T3 (enExample) |
| IL (1) | IL254919B (enExample) |
| SG (1) | SG11201708336YA (enExample) |
| WO (1) | WO2016161516A1 (enExample) |
Families Citing this family (102)
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| US11208649B2 (en) | 2015-12-07 | 2021-12-28 | Zymergen Inc. | HTP genomic engineering platform |
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| CA2996982A1 (en) | 2015-09-23 | 2017-03-30 | Universite Laval | Modification of the dystrophin gene and uses thereof |
| WO2017175072A1 (en) | 2016-04-08 | 2017-10-12 | Feldan Bio Inc. | Peptide shuttle based gene disruption |
| WO2018053632A1 (en) | 2016-09-23 | 2018-03-29 | UNIVERSITé LAVAL | Methods of modifying the dystrophin gene and restoring dystrophin expression and uses thereof |
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| IL265920B2 (en) | 2016-10-12 | 2024-05-01 | Feldan Bio Inc | Rationally-designed synthetic peptide shuttle agents for delivering polypeptide cargos from an extracellular space to the cytosol and/or nucleus of a target eukaryotic cell, uses thereof, methods and kits relating to same |
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