CA3111386A1 - Cellulose-synthase-like enzymes and uses thereof - Google Patents

Cellulose-synthase-like enzymes and uses thereof Download PDF

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Publication number
CA3111386A1
CA3111386A1 CA3111386A CA3111386A CA3111386A1 CA 3111386 A1 CA3111386 A1 CA 3111386A1 CA 3111386 A CA3111386 A CA 3111386A CA 3111386 A CA3111386 A CA 3111386A CA 3111386 A1 CA3111386 A1 CA 3111386A1
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CA
Canada
Prior art keywords
plant
cell
metabolite
derivative
genus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3111386A
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English (en)
French (fr)
Inventor
Asaph Aharoni
Prashant SONAWANE
Maxim ITKIN
Adam JOZWIAK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yeda Research and Development Co Ltd
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Yeda Research and Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US16/123,248 external-priority patent/US10806119B2/en
Priority claimed from IL268269A external-priority patent/IL268269A/en
Application filed by Yeda Research and Development Co Ltd filed Critical Yeda Research and Development Co Ltd
Publication of CA3111386A1 publication Critical patent/CA3111386A1/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3111386A 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof Pending CA3111386A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16/123,248 2018-09-06
US16/123,248 US10806119B2 (en) 2013-06-05 2018-09-06 Plant with altered content of steroidal alkaloids
IL268269 2019-07-25
IL268269A IL268269A (en) 2019-07-25 2019-07-25 Production of high-value saponins by the use of cellulose-synthase-like family enzymes
PCT/IL2019/051000 WO2020049572A1 (en) 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof

Publications (1)

Publication Number Publication Date
CA3111386A1 true CA3111386A1 (en) 2020-03-12

Family

ID=69721605

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3111386A Pending CA3111386A1 (en) 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof

Country Status (8)

Country Link
US (1) US12041907B2 (https=)
EP (1) EP3847250A1 (https=)
JP (1) JP2022500023A (https=)
CN (1) CN112969785A (https=)
CA (1) CA3111386A1 (https=)
IL (1) IL281171A (https=)
SG (1) SG11202102106RA (https=)
WO (1) WO2020049572A1 (https=)

Cited By (1)

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CN113430194A (zh) * 2020-11-30 2021-09-24 东北林业大学 一种基于CRISPR/Cas9的白桦基因编辑方法

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EP4474478A3 (en) * 2019-10-17 2025-04-09 Osaka University Glucuronyltransferase, gene encoding same and method for using same
WO2023212651A2 (en) * 2022-04-27 2023-11-02 The Regents Of The University Of Colorado A Body Corporate Enhanced production of squalene in plant systems
GB202209501D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
GB202209588D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Methods and compositions
WO2024047057A1 (en) * 2022-09-01 2024-03-07 Vib Vzw Means and methods to produce triterpene saponins in eukaryotic cells
WO2024108582A1 (zh) * 2022-11-25 2024-05-30 中国科学院深圳先进技术研究院 重组菌及其构建方法和在制备皂皮酸中的应用
CN119552915B (zh) * 2025-02-06 2025-05-06 杭州师范大学 Wrd1基因在水稻生长发育调控中的应用
CN120249324A (zh) * 2025-03-28 2025-07-04 南京农业大学 大豆高产耐密植抗倒伏耐旱基因GmCSLG1及其应用
CN120173079B (zh) * 2025-05-20 2025-08-26 云南师范大学 ScGAME9基因在提高马铃薯抗寒性中的应用
CN120230767B (zh) * 2025-05-29 2025-09-02 江西农业大学 调控薯蓣皂苷合成的DpCYP707A2基因及应用
CN120905346B (zh) * 2025-10-11 2025-12-26 武汉糖智药业有限公司 Qs21中间体的制备方法

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113430194A (zh) * 2020-11-30 2021-09-24 东北林业大学 一种基于CRISPR/Cas9的白桦基因编辑方法
CN113430194B (zh) * 2020-11-30 2023-04-07 东北林业大学 一种基于CRISPR/Cas9的白桦基因编辑方法

Also Published As

Publication number Publication date
WO2020049572A1 (en) 2020-03-12
EP3847250A1 (en) 2021-07-14
SG11202102106RA (en) 2021-04-29
CN112969785A (zh) 2021-06-15
JP2022500023A (ja) 2022-01-04
US12041907B2 (en) 2024-07-23
US20220217934A1 (en) 2022-07-14
IL281171A (en) 2021-04-29

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