JP2022500023A - セルロース合成酵素様酵素及びその使用 - Google Patents

セルロース合成酵素様酵素及びその使用 Download PDF

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JP2022500023A
JP2022500023A JP2021512835A JP2021512835A JP2022500023A JP 2022500023 A JP2022500023 A JP 2022500023A JP 2021512835 A JP2021512835 A JP 2021512835A JP 2021512835 A JP2021512835 A JP 2021512835A JP 2022500023 A JP2022500023 A JP 2022500023A
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plant
seq
acid sequence
compound
nucleic acid
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JP2022500023A5 (https=
Inventor
アハロニ、アサフ
ソナワネ、プラシャント
イトキン、マクシム
ヨズヴィアク、アダム
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イエダ リサーチ アンド ディベロップメント カンパニー リミテッド
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Priority claimed from US16/123,248 external-priority patent/US10806119B2/en
Priority claimed from IL268269A external-priority patent/IL268269A/en
Application filed by イエダ リサーチ アンド ディベロップメント カンパニー リミテッド filed Critical イエダ リサーチ アンド ディベロップメント カンパニー リミテッド
Publication of JP2022500023A publication Critical patent/JP2022500023A/ja
Publication of JP2022500023A5 publication Critical patent/JP2022500023A5/ja
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C12N9/1059—Cellulose synthases (2.4.1.12; 2.4.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01017—Glucuronosyltransferase (2.4.1.17)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Virology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2021512835A 2018-09-06 2019-09-05 セルロース合成酵素様酵素及びその使用 Pending JP2022500023A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US16/123,248 2018-09-06
US16/123,248 US10806119B2 (en) 2013-06-05 2018-09-06 Plant with altered content of steroidal alkaloids
IL268269 2019-07-25
IL268269A IL268269A (en) 2019-07-25 2019-07-25 Production of high-value saponins by the use of cellulose-synthase-like family enzymes
PCT/IL2019/051000 WO2020049572A1 (en) 2018-09-06 2019-09-05 Cellulose-synthase-like enzymes and uses thereof

Publications (2)

Publication Number Publication Date
JP2022500023A true JP2022500023A (ja) 2022-01-04
JP2022500023A5 JP2022500023A5 (https=) 2022-11-07

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JP2021512835A Pending JP2022500023A (ja) 2018-09-06 2019-09-05 セルロース合成酵素様酵素及びその使用

Country Status (8)

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US (1) US12041907B2 (https=)
EP (1) EP3847250A1 (https=)
JP (1) JP2022500023A (https=)
CN (1) CN112969785A (https=)
CA (1) CA3111386A1 (https=)
IL (1) IL281171A (https=)
SG (1) SG11202102106RA (https=)
WO (1) WO2020049572A1 (https=)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201909104D0 (en) 2019-06-25 2019-08-07 Plant Bioscience Ltd Transferase enzymes
EP4474478A3 (en) * 2019-10-17 2025-04-09 Osaka University Glucuronyltransferase, gene encoding same and method for using same
CN113430194B (zh) * 2020-11-30 2023-04-07 东北林业大学 一种基于CRISPR/Cas9的白桦基因编辑方法
WO2023212651A2 (en) * 2022-04-27 2023-11-02 The Regents Of The University Of Colorado A Body Corporate Enhanced production of squalene in plant systems
GB202209501D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Biosynthetic enzymes
GB202209588D0 (en) * 2022-06-29 2022-08-10 Plant Bioscience Ltd Methods and compositions
WO2024047057A1 (en) * 2022-09-01 2024-03-07 Vib Vzw Means and methods to produce triterpene saponins in eukaryotic cells
WO2024108582A1 (zh) * 2022-11-25 2024-05-30 中国科学院深圳先进技术研究院 重组菌及其构建方法和在制备皂皮酸中的应用
CN119552915B (zh) * 2025-02-06 2025-05-06 杭州师范大学 Wrd1基因在水稻生长发育调控中的应用
CN120249324A (zh) * 2025-03-28 2025-07-04 南京农业大学 大豆高产耐密植抗倒伏耐旱基因GmCSLG1及其应用
CN120173079B (zh) * 2025-05-20 2025-08-26 云南师范大学 ScGAME9基因在提高马铃薯抗寒性中的应用
CN120230767B (zh) * 2025-05-29 2025-09-02 江西农业大学 调控薯蓣皂苷合成的DpCYP707A2基因及应用
CN120905346B (zh) * 2025-10-11 2025-12-26 武汉糖智药业有限公司 Qs21中间体的制备方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012095843A1 (en) * 2011-01-13 2012-07-19 Yeda Research And Development Co. Ltd. Organisms with altered steroidal saponin and steroidal alkaloid levels and methods for producing same
WO2014195944A1 (en) * 2013-06-05 2014-12-11 Yeda Research And Development Co. Ltd. Plant with altered content of steroidal glycoalkaloids

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5807718A (en) 1994-12-02 1998-09-15 The Scripps Research Institute Enzymatic DNA molecules
US5959180A (en) 1997-02-07 1999-09-28 The United States Of America As Represented By The Secretary Of Agriculture DNA sequences from potato encoding solanidine UDP-glucose glucosyltransferase and use to reduce glycoalkaloids in solanaceous plants
IL134446A0 (en) 1997-09-05 2001-04-30 Pentapharm Ag Expression vector for improved production of polypeptides in yeast
DE19919550A1 (de) 1999-04-29 2000-11-02 Norddeutsche Pflanzenzucht Han Verfahren zur Erzeugung von Organismen mit erhöhter Sterolglykosid-Produktion und Verwendung der Sterolglykoside
US6562595B2 (en) 2000-02-18 2003-05-13 Mcgill University Dominant selectable marker for gene transformation and disruption in yeasts
US20120094385A1 (en) 2000-07-31 2012-04-19 Kuehnle Agrosystems, Inc. Gene positioning system for plastidic transformation and products thereof
KR100430785B1 (ko) 2001-06-21 2004-05-10 송재만 영양요구성 우성유전자를 함유하는 효모 형질전환 벡터, 이를 함유하는 효모 형질전환체 및 그 제조방법
US20050108791A1 (en) 2001-12-04 2005-05-19 Edgerton Michael D. Transgenic plants with improved phenotypes
WO2004035734A2 (en) 2002-10-15 2004-04-29 Syngenta Participations Ag Plastid transformation
CA2594053A1 (en) 2004-12-24 2006-06-29 Anne Rae Vacuole targeting peptide and nucleic acid
US20090070897A1 (en) 2006-01-12 2009-03-12 Goldman Barry S Genes and uses for plant improvement
US7439419B1 (en) 2005-11-14 2008-10-21 The United States Of America As Represented By The Secretary Of Agriculture Solanum tuberosum β-solanine/β-chaconine rhamnosyl transferase sequences and uses thereof
US7375259B1 (en) 2005-11-14 2008-05-20 The United States Of America, As Represented By The Secretary Of Agriculture Solanum tuberosum sterol alkaloid glycosyltransferase (SGT) a novel solanidine glucosyltransferase SGT2 and uses thereof
JP4737531B2 (ja) 2005-12-05 2011-08-03 サントリーホールディングス株式会社 形質転換酵母を用いるセラミドの製造方法
EP1854878A1 (en) 2006-05-10 2007-11-14 Cosmoferm B.V. Methods and materials for the transformation of the yeast Pichia ciferrii
WO2009009142A2 (en) 2007-07-10 2009-01-15 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
JP2009034036A (ja) 2007-08-01 2009-02-19 Toyota Motor Corp 形質転換用酵母、形質転換方法及び物質生産方法
BRPI0910286A2 (pt) 2008-03-03 2015-09-29 Abbott Lab métodos para transformação de levedura
KR102256410B1 (ko) 2008-04-08 2021-05-26 슬로안-케테링인스티튜트퍼캔서리서치 트리테르펜 사포닌, 그의 합성 방법, 및 그의 용도
BRPI0823018A2 (pt) * 2008-09-04 2019-02-26 Suntory Holdings Ltd polinucleotídeo, proteína codificada pelo polinucleotídeo, vetor, transformante, métodos para produzir a proteína e para produzir um conjugado glicuronídeo.
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
CN102292440B (zh) 2009-01-23 2014-02-12 国立大学法人高知大学 一种用于遗传转化藻类的新型启动子
KR20100095125A (ko) 2009-02-20 2010-08-30 주식회사 포비커 위치 기반 광고 시스템
US20120034698A1 (en) 2009-04-02 2012-02-09 Rosetta Green Ltd. Compositions and methods for increasing oil content in algae
WO2011025011A1 (ja) 2009-08-28 2011-03-03 キリンホールディングス株式会社 グリコアルカロイド生合成酵素活性を有するタンパク質とそれをコードする遺伝子
US20120227134A1 (en) 2009-11-17 2012-09-06 Basf Plant Science Company Gmbh Plants with Increased Yield
CN102753687B (zh) 2010-02-17 2014-05-07 国立大学法人高知大学 用于遗传转化藻类的新型启动子
JP5794770B2 (ja) 2010-08-31 2015-10-14 キリンホールディングス株式会社 グリコアルカロイド生合成酵素活性を有するタンパク質とそれをコードする遺伝子
US9994883B2 (en) 2012-05-11 2018-06-12 Vib Vzw Triterpenoid sapogenin production in plant and microbial cultures
US20160046912A1 (en) * 2013-04-04 2016-02-18 Riken Glucuronosyltransferase, gene encoding same and use thereof
US10806119B2 (en) * 2013-06-05 2020-10-20 Yeda Research And Development Co. Ltd. Plant with altered content of steroidal alkaloids
JP5721191B2 (ja) 2013-07-02 2015-05-20 国立大学法人京都大学 ３段階方式矩形波多重パルスを利用した藻類細胞への遺伝子導入法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012095843A1 (en) * 2011-01-13 2012-07-19 Yeda Research And Development Co. Ltd. Organisms with altered steroidal saponin and steroidal alkaloid levels and methods for producing same
WO2014195944A1 (en) * 2013-06-05 2014-12-11 Yeda Research And Development Co. Ltd. Plant with altered content of steroidal glycoalkaloids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
"ステロイドサポニン生合成機構の解明", KAKEN- 研究課題をさがす, 2016年, JPN6022015336, pages 1 - 4, ISSN: 0005121811 *
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013年, vol. 61, JPN6022015335, pages 9534 - 9550, ISSN: 0005121812 *
SCIENCE, 2013年, vol. 341, JPN6022015334, pages 175 - 179, ISSN: 0004761050 *
SCIENCE, 2013年, vol. Vol.341 (6142), JPN6023000698, pages 175 - 179, ISSN: 0004966111 *

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Publication number Publication date
CA3111386A1 (en) 2020-03-12
WO2020049572A1 (en) 2020-03-12
EP3847250A1 (en) 2021-07-14
SG11202102106RA (en) 2021-04-29
CN112969785A (zh) 2021-06-15
US12041907B2 (en) 2024-07-23
US20220217934A1 (en) 2022-07-14
IL281171A (en) 2021-04-29

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