CA3096342C - Nucleic acid amplifications - Google Patents

Nucleic acid amplifications Download PDF

Info

Publication number
CA3096342C
CA3096342C CA3096342A CA3096342A CA3096342C CA 3096342 C CA3096342 C CA 3096342C CA 3096342 A CA3096342 A CA 3096342A CA 3096342 A CA3096342 A CA 3096342A CA 3096342 C CA3096342 C CA 3096342C
Authority
CA
Canada
Prior art keywords
temperature
amplification
mixture
reagents
ion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA3096342A
Other languages
English (en)
French (fr)
Other versions
CA3096342A1 (en
Inventor
Honghua Zhang
Jarrod Provins
Richard Roth
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ionian Technologies LLC
Original Assignee
Ionian Technologies LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ionian Technologies LLC filed Critical Ionian Technologies LLC
Publication of CA3096342A1 publication Critical patent/CA3096342A1/en
Application granted granted Critical
Publication of CA3096342C publication Critical patent/CA3096342C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3096342A 2012-06-08 2013-06-07 Nucleic acid amplifications Active CA3096342C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261657227P 2012-06-08 2012-06-08
US61/657,227 2012-06-08
US201361782199P 2013-03-14 2013-03-14
US61/782,199 2013-03-14
CA2876159A CA2876159C (en) 2012-06-08 2013-06-07 Nucleic acid amplifications

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2876159A Division CA2876159C (en) 2012-06-08 2013-06-07 Nucleic acid amplifications

Publications (2)

Publication Number Publication Date
CA3096342A1 CA3096342A1 (en) 2013-12-12
CA3096342C true CA3096342C (en) 2023-04-04

Family

ID=49712703

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3096342A Active CA3096342C (en) 2012-06-08 2013-06-07 Nucleic acid amplifications
CA2876159A Active CA2876159C (en) 2012-06-08 2013-06-07 Nucleic acid amplifications

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2876159A Active CA2876159C (en) 2012-06-08 2013-06-07 Nucleic acid amplifications

Country Status (8)

Country Link
US (3) US10927393B2 (2)
EP (2) EP2859111B1 (2)
JP (4) JP6861465B2 (2)
CN (2) CN104662159B (2)
AU (4) AU2013271404B2 (2)
CA (2) CA3096342C (2)
ES (1) ES2823551T3 (2)
WO (1) WO2013185081A1 (2)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2859111B1 (en) 2012-06-08 2020-07-08 Ionian Technologies, LLC Nucleic acid amplifications
US9850525B2 (en) 2014-01-29 2017-12-26 Agilent Technologies, Inc. CAS9-based isothermal method of detection of specific DNA sequence
EP3099810B1 (en) 2014-01-31 2019-05-01 Qiagen GmbH Cation chelator hot start
WO2016004333A2 (en) * 2014-07-02 2016-01-07 Promega Corporation Reversible metal ion chelators
CN107208136A (zh) * 2014-12-18 2017-09-26 通用电气医疗集团英国有限公司 通过与免疫测定法偶联的核酸扩增的固体支持物上的分析物检测
GB201515355D0 (en) * 2015-08-28 2015-10-14 Ge Healthcare Ltd A method and kit for analyte detection
US10329601B2 (en) * 2015-12-28 2019-06-25 Ionian Technologies, Inc. Nicking and extension amplification reaction (NEAR) of Streptococcus species
US11118206B2 (en) 2016-01-13 2021-09-14 The Trustees Of The University Of Pennsylvania Multiple stage isothermal enzymatic amplification
WO2017141067A1 (en) 2016-02-16 2017-08-24 The University Of Tokyo Method of eliminating background amplification of nucleic acid targets
US11299777B2 (en) 2016-04-04 2022-04-12 Nat Diagnostics, Inc. Isothermal amplification components and processes
US9617587B1 (en) 2016-04-04 2017-04-11 Nat Diagnostics, Inc. Isothermal amplification components and processes
CN106093415B (zh) * 2016-04-29 2017-12-19 浙江大学 一种蛋白实时定量检测方法
CN106053403B (zh) * 2016-04-29 2019-05-03 浙江大学 一种蛋白定量检测方法
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
BR112019017947A2 (pt) * 2017-02-28 2020-05-19 Alere San Diego Inc dispositivo microfluídico, leitor configurado para recebê-lo e método
GB2569965A (en) * 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
CN111979303A (zh) * 2020-08-11 2020-11-24 上海奕谱生物科技有限公司 一种核酸检测试剂盒、方法及其应用
WO2025171104A1 (en) * 2024-02-08 2025-08-14 Alere San Diego, Inc. Universal hairpin probes

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009944A2 (en) * 1989-12-22 1991-07-11 F.Hoffmann-La Roche Ag High temperature reverse transcriptases
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5556751A (en) 1991-04-25 1996-09-17 Amoco Corporation Selective amplification system using Q-β replicase
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US5733733A (en) 1992-08-04 1998-03-31 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5834202A (en) 1992-08-04 1998-11-10 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5614389A (en) 1992-08-04 1997-03-25 Replicon, Inc. Methods for the isothermal amplification of nucleic acid molecules
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5942391A (en) 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5916779A (en) 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US5731150A (en) 1995-11-01 1998-03-24 Chiron Diagnostic Corporation IS6110 based molecular detection of mycobacterium tuberculosis
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
WO1998045474A1 (en) 1997-04-04 1998-10-15 Innogenetics N.V. Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions
US6245506B1 (en) * 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device
CN1099464C (zh) * 2000-11-28 2003-01-22 复旦大学 一种端粒酶活性检测方法
US6448085B1 (en) * 2001-04-13 2002-09-10 Sysmex Corporation Quality control material and calibrator for nucleated red blood cell tested on hematology analyzer
US20020172972A1 (en) * 2001-05-15 2002-11-21 President And Fellows Of Harvard College Use of a selectively inactivatable enzyme to digest contaminating nucleic acid
US20030138800A1 (en) 2001-07-15 2003-07-24 Keck Graduate Institute Exponential amplification of nucleic acids using nicking agents
WO2003008624A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
EP1419275A4 (en) 2001-08-02 2007-05-30 Wayne M Barnes HOT START METHOD USING MAGNESIUM PRECIPITATE FOR MOLECULAR MANIPULATION OF NUCLEIC ACIDS
US6403341B1 (en) 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US8030000B2 (en) * 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7270981B2 (en) 2002-02-21 2007-09-18 Asm Scientific, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
US20040038213A1 (en) 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
CA2498764C (en) 2002-09-20 2015-11-10 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
US7662594B2 (en) 2002-09-20 2010-02-16 New England Biolabs, Inc. Helicase-dependent amplification of RNA
WO2005017173A1 (en) * 2003-07-29 2005-02-24 Sigma Aldrich Co. Methods and compositions for amplification of dna
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
GB2416352B (en) 2004-07-21 2009-01-28 Bioline Ltd A method for performing the hot start of enzymatic reactions
WO2007096702A2 (en) 2005-07-25 2007-08-30 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
JP5106416B2 (ja) * 2006-01-06 2012-12-26 アジレント・テクノロジーズ・インク 濃縮（ｐａｃｋｅｄ）ＤＮＡポリメラーゼを含む核酸複製のための反応緩衝液組成物
CN101421417B (zh) * 2006-02-27 2013-04-03 霍夫曼-拉罗奇有限公司 经由镁螯合的pcr热启动
US8071308B2 (en) 2006-05-04 2011-12-06 Alere San Diego, Inc. Recombinase polymerase amplification
US9481912B2 (en) * 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
CA2687375C (en) 2007-05-18 2017-08-29 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services, Centers For Disease Control And Prevention Primers and probes for the detection of streptococcus pneumoniae
US9689031B2 (en) * 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
KR101414713B1 (ko) * 2007-10-11 2014-07-03 삼성전자주식회사 리가제 및 엔도뉴클레아제의 존재하에서 롤링서클 증폭에의하여 표적 핵산을 증폭하는 방법
JP2009139487A (ja) 2007-12-04 2009-06-25 Nippon Sheet Glass Co Ltd 正立等倍レンズアレイプレート
US8911948B2 (en) * 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
JP5572943B2 (ja) * 2008-12-17 2014-08-20 株式会社島津製作所 血液検体からのｄｎａ増幅方法及びｄｎａ増幅キット
JP5720564B2 (ja) * 2009-03-31 2015-05-20 凸版印刷株式会社 遺伝子型の識別方法
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
EP2464738A4 (en) * 2009-08-12 2013-05-01 Nugen Technologies Inc METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF MATRIX NUCLEIC ACID
CA2786473A1 (en) 2010-01-08 2011-07-14 Qiagen Gaithersburg, Inc. Materials and methods for isothermal nucleic acid amplification
EP2714931A1 (en) * 2011-06-03 2014-04-09 Genomic Vision Assessment of cancer risk based on rnu2 cnv and interplay between rnu2 and brca1
WO2013059746A1 (en) * 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013101783A2 (en) * 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
KR101870311B1 (ko) 2012-03-09 2018-06-25 (주)바이오니아 핫스타트 역전사반응 또는 핫스타트 역전사 중합효소 연쇄반응용 조성물
EP2859111B1 (en) 2012-06-08 2020-07-08 Ionian Technologies, LLC Nucleic acid amplifications
EP3099810B1 (en) * 2014-01-31 2019-05-01 Qiagen GmbH Cation chelator hot start
WO2016004333A2 (en) * 2014-07-02 2016-01-07 Promega Corporation Reversible metal ion chelators

Also Published As

Publication number Publication date
JP2019115344A (ja) 2019-07-18
US10927393B2 (en) 2021-02-23
CA2876159C (en) 2022-09-06
US20250277247A1 (en) 2025-09-04
JP2021072833A (ja) 2021-05-13
EP2859111A4 (en) 2016-04-27
AU2017228698B2 (en) 2019-06-27
AU2013271404A1 (en) 2014-12-18
CN104662159B (zh) 2021-10-26
JP2024109739A (ja) 2024-08-14
CA2876159A1 (en) 2013-12-12
AU2013271404B2 (en) 2017-10-12
AU2022200787A1 (en) 2022-02-24
JP6861465B2 (ja) 2021-04-21
CA3096342A1 (en) 2013-12-12
WO2013185081A1 (en) 2013-12-12
EP3778915A1 (en) 2021-02-17
ES2823551T3 (es) 2021-05-07
EP2859111A1 (en) 2015-04-15
EP2859111B1 (en) 2020-07-08
US20210155965A1 (en) 2021-05-27
CN104662159A (zh) 2015-05-27
JP2015518735A (ja) 2015-07-06
AU2022200787B2 (en) 2024-03-28
US20130330777A1 (en) 2013-12-12
CN114214394A (zh) 2022-03-22
AU2019236751A1 (en) 2019-10-17
AU2017228698A1 (en) 2017-10-05

Similar Documents

Publication Publication Date Title
AU2022200787B2 (en) Nucleic acid amplifications
EP3252172B1 (en) Fast hybridization for next generation sequencing target enrichment
JP6966681B2 (ja) 限られたヌクレオチド組成を有するプライマーを用いた増幅
US20170335379A1 (en) Detection of nucleic acids in crude matrices
US20160024558A1 (en) Nucleic acid binding proteins and uses thereof
US10633697B2 (en) Caution chelator hot start
HK40070420A (en) Nucleic acid amplifications
US20220325337A1 (en) Methods for asymmetric semi-nested isothermal nucleotide amplification
JP2024509834A (ja) 新興感染症のポイントオブニード診断のための保護された等温核酸増幅（ｐｉｎａ）方法

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20210112

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 12TH ANNIV.) - STANDARD

Year of fee payment: 12

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250509

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250509