CA3093811A1 - Methods and apparatus for single biological nanoparticle analysis - Google Patents

Methods and apparatus for single biological nanoparticle analysis Download PDF

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Publication number
CA3093811A1
CA3093811A1 CA3093811A CA3093811A CA3093811A1 CA 3093811 A1 CA3093811 A1 CA 3093811A1 CA 3093811 A CA3093811 A CA 3093811A CA 3093811 A CA3093811 A CA 3093811A CA 3093811 A1 CA3093811 A1 CA 3093811A1
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CA
Canada
Prior art keywords
less
biological
nanometers
nanoparticle
nanoparticles
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3093811A
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English (en)
French (fr)
Inventor
Daniel T. Chiu
Seung Ryoung JUNG
Jingang Wang
Bryant S. Fujimoto
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
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University of Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
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Application filed by University of Washington filed Critical University of Washington
Publication of CA3093811A1 publication Critical patent/CA3093811A1/en
Pending legal-status Critical Current

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502776—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for focusing or laminating flows
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0654—Lenses; Optical fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y35/00—Methods or apparatus for measurement or analysis of nanostructures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2826—Collecting by adsorption or absorption
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1493—Particle size

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3093811A 2018-04-13 2019-03-29 Methods and apparatus for single biological nanoparticle analysis Pending CA3093811A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862657278P 2018-04-13 2018-04-13
US62/657,278 2018-04-13
PCT/US2019/025033 WO2019199499A1 (en) 2018-04-13 2019-03-29 Methods and apparatus for single biological nanoparticle analysis

Publications (1)

Publication Number Publication Date
CA3093811A1 true CA3093811A1 (en) 2019-10-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA3093811A Pending CA3093811A1 (en) 2018-04-13 2019-03-29 Methods and apparatus for single biological nanoparticle analysis

Country Status (6)

Country Link
US (2) US12447469B2 (https=)
EP (1) EP3775838A4 (https=)
JP (1) JP7344569B2 (https=)
CN (1) CN112105912B (https=)
CA (1) CA3093811A1 (https=)
WO (1) WO2019199499A1 (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2943778B1 (en) 2013-01-09 2020-10-28 The Regents of The University of California Apparatus and methods for fluorescence imaging using radiofrequency-multiplexed excitation
EP3120130B1 (en) 2014-03-18 2023-07-26 The Regents of the University of California Parallel flow cytometer using radiofrequency mulitplexing, and method
CN108474728B (zh) 2015-10-13 2022-04-26 贝克顿·迪金森公司 多模态荧光成像流式细胞术系统
JP7130562B2 (ja) 2016-03-17 2022-09-05 ベクトン・ディキンソン・アンド・カンパニー ハイスループット蛍光フローサイトメーターを用いる細胞選別
US11852578B2 (en) * 2018-07-20 2023-12-26 Sony Corporation Microparticle measurement spectrometer, microparticle measurement device using the microparticle measurement spectrometer, and method for calibrating microparticle measurement photoelectric conversion system
AU2019343188A1 (en) * 2018-09-21 2021-05-20 Aufbau Medical Innovations Limited Compositions and methods for glaucoma
JP2020134341A (ja) * 2019-02-20 2020-08-31 シスメックス株式会社 測定成否判定方法および試料測定装置
EP3997439A4 (en) 2019-07-10 2023-07-19 Becton, Dickinson and Company Reconfigurable integrated circuits for adjusting cell sorting classification
US20210109094A1 (en) * 2019-10-11 2021-04-15 Case Western Reserve University Detection of disease components using magnetic particles and microfluidics
JP7541751B2 (ja) * 2020-01-22 2024-08-29 シンクサイト株式会社 フローサイトメータ用フローセルおよびフローサイトメータ用フローセルの洗浄方法
CN111537480B (zh) * 2020-04-26 2023-05-02 中央民族大学 基于单分子全内反射荧光成像技术的病毒快速检测方法
US12553885B2 (en) 2020-05-07 2026-02-17 Hand Held Products, Inc. Apparatuses, systems, and methods for sample testing
US12498328B2 (en) 2020-05-07 2025-12-16 Hand Held Products, Inc. Apparatuses, systems, and methods for sample testing
US12196672B2 (en) 2020-05-07 2025-01-14 Hand Held Products, Inc. Apparatuses, systems, and methods for sample testing
DE102020116547A1 (de) 2020-06-23 2021-12-23 Abberior Instruments Gmbh Verfahren zur Lokalisation einzelner Moleküle eines Farbstoffs in einer Probe und zum Erzeugen hochaufgelöster Bilder einer Struktur in einer Probe
EP4188591A4 (en) * 2020-07-31 2024-08-07 12198703 Canada Ltd. DIRECTIONAL CHEMICAL KINETICS
CN116888273A (zh) * 2020-10-19 2023-10-13 富默乐国际控股有限公司 用于样本至结果pcr分析的具有流体通道几何形状的装置及其使用方法
US11846574B2 (en) 2020-10-29 2023-12-19 Hand Held Products, Inc. Apparatuses, systems, and methods for sample capture and extraction
US12313519B2 (en) 2020-11-10 2025-05-27 University Of Washington Method and apparatus for flow-based, single-particle and/or single-molecule analysis
WO2022172208A1 (en) * 2021-02-11 2022-08-18 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Flow virometer for rapid detection of intact viruses
CN113075176B (zh) * 2021-03-17 2022-11-01 西安医学院 肿瘤标志物多重分析装置
CN113654953A (zh) * 2021-07-29 2021-11-16 山东大学深圳研究院 一种检测纳米颗粒污染物环境行为和生物效应的方法
CN115728213A (zh) * 2021-08-31 2023-03-03 贝克曼库尔特有限公司 用于纳米粒子的检测系统和样本处理仪
CN114686592B (zh) * 2022-05-30 2022-09-09 广东忠信生物科技有限公司 一种多类型靶标联合检测的试剂盒及制备方法、使用方法
WO2024015388A2 (en) * 2022-07-12 2024-01-18 The Johns Hopkins University Polysiloxane-based devices with reduced drug absorption characteristics
CN116124544B (zh) * 2022-12-13 2025-11-04 浙江省肿瘤医院 一种应用于液体活检样品的光电富集系统
PL249105B1 (pl) * 2023-06-28 2026-03-02 Inst Chemii Fizycznej Polskiej Akademii Nauk Układ mikroprzepływowy z zintegrowanym światłowodem jednomodowym
TWI883493B (zh) * 2023-07-27 2025-05-11 醫華生技股份有限公司 生物微粒富集設備、生物微粒富集裝置、及微滴產生器
US20250114799A1 (en) * 2023-10-10 2025-04-10 Exokeryx, Inc. Multistage Dielectrophoretic Filter System for Extracellular Vesicles

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6214563B1 (en) * 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
AU774638B2 (en) 2000-02-16 2004-07-01 Wisconsin Alumni Research Foundation Method and apparatus for detection of microscopic pathogens
US6968463B2 (en) 2001-01-17 2005-11-22 Hewlett-Packard Development Company, L.P. System for controlling access to resources in a storage area network
JP4357138B2 (ja) 2001-06-13 2009-11-04 トヨタ自動車株式会社 粒子測定方法及びその装置
US20030059822A1 (en) * 2001-09-18 2003-03-27 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
US20050095602A1 (en) * 2003-11-04 2005-05-05 West Jason A. Microfluidic integrated microarrays for biological detection
US7471393B2 (en) 2004-03-06 2008-12-30 Michael Trainer Methods and apparatus for determining the size and shape of particles
US7075647B2 (en) * 2004-06-30 2006-07-11 Beckman Coulter, Inc. Back-scatter detection in flow cytometers
US7279980B2 (en) * 2005-04-28 2007-10-09 Regents Of The University Of California Non-uniform distributed multi-stage circuits
US8173413B2 (en) 2005-08-11 2012-05-08 University Of Washington Separation and concentration of biological cells and biological particles using a one-dimensional channel
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US20090323061A1 (en) 2006-02-28 2009-12-31 Lukas Novotny Multi-color hetereodyne interferometric apparatus and method for sizing nanoparticles
US20070229823A1 (en) * 2006-03-31 2007-10-04 Intel Corporation Determination of the number concentration and particle size distribution of nanoparticles using dark-field microscopy
EP2021766A2 (en) 2006-05-17 2009-02-11 Luminex Corporation Chip-based flow cytometer type systems for analyzing fluorescently tagged particles
MX2009007101A (es) 2006-12-29 2009-12-01 Univ Washington Superficies y materiales antibioincrustación bifuncionales.
CA2682275C (en) * 2007-03-28 2017-05-09 Bionanomatrix, Inc. Methods of macromolecular analysis using nanochannel arrays
US8841135B2 (en) 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
KR20080114018A (ko) 2007-06-26 2008-12-31 삼성전자주식회사 잉크젯 화상형성장치 및 그 제어방법
DE102007030347A1 (de) * 2007-06-29 2009-01-02 Ducrée, Jens, Dr. Integrierter Rotor
WO2009021215A1 (en) 2007-08-09 2009-02-12 Celula, Inc. Methods and devices for correlated, multi-parameter single cell measurements and recovery of remnant biological material
EP2232229B1 (en) * 2007-12-04 2021-02-17 Particle Measuring Systems, Inc. Two-dimensional optical imaging methods for particle detection
WO2009151505A1 (en) * 2008-04-11 2009-12-17 China Molecular, Ltd. Devices and methods for immunoglobulin production
WO2009128948A1 (en) 2008-04-17 2009-10-22 Peptimmune, Inc. Design and synthesis of directed sequence polymer compositions and antibodies thereof for the treatment of protein conformational disorders
KR101102532B1 (ko) * 2008-07-10 2012-01-03 삼성전자주식회사 시약 카트리지, 시약 카트리지를 구비하는 미세유동장치, 그 제조방법, 및 이를 이용한 시료분석방법
WO2010013704A1 (ja) 2008-07-29 2010-02-04 シャープ株式会社 マイクロデバイス及びマイクロチップ装置並びにこれらを用いた分析方法
TW201017149A (en) 2008-08-06 2010-05-01 Invitrox Inc Use of focused light scattering techniques in biological applications
WO2010062350A1 (en) 2008-10-31 2010-06-03 Biomerieux, Inc. Methods for detection, characterization and/or identification of microorganisms in a sealed container
US8248609B2 (en) * 2008-11-04 2012-08-21 The Johns Hopkins University Cylindrical illumination confocal spectroscopy system
JP2010243374A (ja) 2009-04-08 2010-10-28 Chuo Univ ナノ粒子の粒径測定装置及びナノ粒子の粒径測定方法
WO2010148252A1 (en) * 2009-06-17 2010-12-23 Jody Vykoukal Method and apparatus for quantitative microimaging
CN103097878B (zh) * 2010-09-10 2015-07-22 奥林巴斯株式会社 使用单个发光颗粒的光强度的光学分析方法
US9423335B2 (en) 2011-07-21 2016-08-23 Invitrox, Inc. Instrument and method for optical particle sensing
JP2016527494A (ja) * 2013-07-05 2016-09-08 ユニバーシティ オブ ワシントン スルー イッツ センター フォー コマーシャライゼーション マイクロ流体アッセイのための方法、組成物およびシステム
EP3046543A1 (en) * 2013-09-18 2016-07-27 Stc.Unm Torroidal mesoporous silica nanoparticles (tmsnps) and related protocells
GB201320146D0 (en) * 2013-11-14 2014-01-01 Cambridge Entpr Ltd Fluidic separation and detection
EP3120130B1 (en) 2014-03-18 2023-07-26 The Regents of the University of California Parallel flow cytometer using radiofrequency mulitplexing, and method
US10697007B2 (en) * 2014-06-27 2020-06-30 The Regents Of The University Of California PCR-activated sorting (PAS)
US20160103077A1 (en) * 2014-10-13 2016-04-14 Purewater Medical, Inc. Liquid contaminant sensor system and method
DE112015006729B4 (de) 2015-07-24 2019-05-16 Mitsubishi Electric Corporation Fahrzeugkonstantgeschwindigkeitsbewegungssteuervorrichtung, Fahrzeugkonstantgeschwindigkeitsbewegungssteuerverfahren und Fahrzeugkonstantgeschwindigkeitsbewegungssteuerprogramm
EP3335028A1 (en) * 2015-08-11 2018-06-20 Scintillon Institute for Biomedical and Bioenergy Research Optical analyses of particles and vesicles
WO2017041809A1 (en) 2015-09-07 2017-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method and apparatus for detecting particles, like biological macromolecules or nanoparticles

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Publication number Publication date
EP3775838A1 (en) 2021-02-17
US20260014561A1 (en) 2026-01-15
US12447469B2 (en) 2025-10-21
EP3775838A4 (en) 2022-01-19
CN112105912A (zh) 2020-12-18
US20210016276A1 (en) 2021-01-21
WO2019199499A1 (en) 2019-10-17
JP7344569B2 (ja) 2023-09-14
JP2021521417A (ja) 2021-08-26
CN112105912B (zh) 2025-05-23

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