CA3055900A1 - Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants - Google Patents

Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

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Publication number
CA3055900A1
CA3055900A1 CA3055900A CA3055900A CA3055900A1 CA 3055900 A1 CA3055900 A1 CA 3055900A1 CA 3055900 A CA3055900 A CA 3055900A CA 3055900 A CA3055900 A CA 3055900A CA 3055900 A1 CA3055900 A1 CA 3055900A1
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Canada
Prior art keywords
codon
protein
plant
optimized
ctb
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3055900A
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English (en)
French (fr)
Inventor
Henry Daniell
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University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Publication of CA3055900A1 publication Critical patent/CA3055900A1/en
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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CA3055900A 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants Pending CA3055900A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662310788P 2016-03-20 2016-03-20
US62/310,788 2016-03-20
PCT/US2017/023263 WO2017165320A1 (en) 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

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CA3055900A1 true CA3055900A1 (en) 2017-09-28

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CA3055900A Pending CA3055900A1 (en) 2016-03-20 2017-03-20 Codon optimization and ribosome profiling for increasing transgene expression in chloroplasts of higher plants

Country Status (6)

Country Link
US (1) US11332754B2 (https=)
EP (1) EP3432902B8 (https=)
JP (1) JP7145083B2 (https=)
CN (1) CN110603046B (https=)
CA (1) CA3055900A1 (https=)
WO (1) WO2017165320A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021003952A1 (zh) * 2019-07-08 2021-01-14 清华大学 极小量细胞核糖体保护的rna片段测序的方法

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3454876A4 (en) * 2016-05-12 2019-11-20 The Trustees of The University of Pennsylvania Compositions and methods for inhibiting biofilm deposition and production
CA3110080A1 (en) * 2018-08-22 2020-02-27 The Trustees Of The University Of Pennsylvania Compositions and methods for production of antibiotic free biopharmaceuticals in lettuce chloroplasts
CN111635935A (zh) * 2020-06-10 2020-09-08 华中农业大学 一种低成本、高通量间接量化蛋白表达水平的方法及其应用
CN120266212A (zh) * 2022-11-24 2025-07-04 南京金斯瑞生物科技有限公司 密码子优化
CN117511978B (zh) * 2024-01-08 2024-03-22 深圳粒影生物科技有限公司 一种提高外源蛋白表达量的方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4946676A (en) * 1986-08-27 1990-08-07 De Staat Der Nederlanden Vaccine comprising an immunogenic protein and, as an adjuvant, a substantially non-immunogenic, sequentially homologous peptide
JPH10201483A (ja) * 1996-11-25 1998-08-04 Lion Corp ムタナーゼ遺伝子
US20110179530A1 (en) * 2001-01-23 2011-07-21 University Of Central Florida Research Foundation, Inc. Pharmaceutical Proteins, Human Therapeutics, Human Serum Albumin Insulin, Native Cholera Toxin B Subunit on Transgenic Plastids
US7741536B2 (en) * 1997-08-07 2010-06-22 University Of Central Florida Research Foundation, Inc. Expression of human serum albumin in plastids
NL1009834C2 (nl) 1998-08-10 2000-02-11 Stanislaus Laurens Johan Woute Antistoffen voor toepassing in de gerichte en tijdelijke behandeling van mens en dier.
PL347904A1 (en) * 1998-10-07 2002-04-22 Syngenta Participations Ag Therapeutically active proteins in plants
CN1886512A (zh) * 2002-04-23 2006-12-27 斯克里普斯研究所 多肽在叶绿体中的表达以及用于表达多肽的组合物和方法
JP2005185182A (ja) 2003-12-25 2005-07-14 Lion Corp α−１，３−グルコオリゴ糖の製造方法、該製造方法により得られるα−１，３−グルコオリゴ糖およびこれを含有する製剤組成物
WO2007055789A2 (en) 2005-10-31 2007-05-18 Neose Technologies, Inc. Expression of soluble therapeutic proteins
WO2007133558A2 (en) * 2006-05-09 2007-11-22 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
WO2008120107A2 (en) * 2007-04-03 2008-10-09 Oxyrane (Uk) Limited Glycosylation of molecules
GB0911870D0 (en) * 2009-07-08 2009-08-19 Ucl Business Plc Optimised coding sequence and promoter
US8326547B2 (en) * 2009-10-07 2012-12-04 Nanjingjinsirui Science & Technology Biology Corp. Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm
US20120309939A1 (en) * 2009-11-19 2012-12-06 The Scripps Research Institute Production of Therapeutic Proteins in Photosynthetic Organisms
EP2591101B1 (en) * 2010-07-09 2018-11-07 Bioverativ Therapeutics Inc. Systems for factor viii processing and methods thereof
CN102146344A (zh) * 2010-11-02 2011-08-10 上海师范大学 优化密码子提高外源基因表达量和莱茵衣藻制氢量的方法
US10865419B2 (en) * 2011-10-24 2020-12-15 The Trustees Of The University Of Pennsylvania Orally administered plastid expressed cholera toxin B subunit-exendin 4 as treatment for type 2 diabetes
US20150127317A1 (en) * 2012-05-09 2015-05-07 The Regents Of The University Of California Method for in silico Modeling of Gene Product Expression and Metabolism
SI3889173T1 (sl) * 2013-02-15 2023-11-30 Bioverativ Therapeutics Inc. Optimiran gen dejavnika VIII
LT3068869T (lt) 2013-11-15 2020-10-26 The Trustees Of The University Of Pennsylvania Kompozicijos, skirtos inhibitoriaus prieš faktorių viii susidarymo hemofilinių pacientų organizme slopinimui

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021003952A1 (zh) * 2019-07-08 2021-01-14 清华大学 极小量细胞核糖体保护的rna片段测序的方法

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EP3432902A1 (en) 2019-01-30
EP3432902A4 (en) 2019-08-28
US20190194679A1 (en) 2019-06-27
US11332754B2 (en) 2022-05-17
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JP2019509064A (ja) 2019-04-04
WO2017165320A1 (en) 2017-09-28

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