CA3023025A1 - Method of producing a recombinant protein - Google Patents
Method of producing a recombinant protein Download PDFInfo
- Publication number
- CA3023025A1 CA3023025A1 CA3023025A CA3023025A CA3023025A1 CA 3023025 A1 CA3023025 A1 CA 3023025A1 CA 3023025 A CA3023025 A CA 3023025A CA 3023025 A CA3023025 A CA 3023025A CA 3023025 A1 CA3023025 A1 CA 3023025A1
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- CA
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- Prior art keywords
- sequence
- recombinant
- expression
- recombinant protein
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- 230000035939 shock Effects 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 230000002311 subsequent effect Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- -1 temperature Substances 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- 235000019157 thiamine Nutrition 0.000 description 1
- 239000011721 thiamine Substances 0.000 description 1
- 230000034005 thiol-disulfide exchange Effects 0.000 description 1
- 238000001269 time-of-flight mass spectrometry Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000032258 transport Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- C07K14/245—Escherichia (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662332636P | 2016-05-06 | 2016-05-06 | |
| US62/332,636 | 2016-05-06 | ||
| US201662338045P | 2016-05-18 | 2016-05-18 | |
| US62/338,045 | 2016-05-18 | ||
| PCT/EP2017/060654 WO2017191255A1 (en) | 2016-05-06 | 2017-05-04 | Method of producing a recombinant protein |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3023025A1 true CA3023025A1 (en) | 2017-11-09 |
Family
ID=58765814
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3023025A Abandoned CA3023025A1 (en) | 2016-05-06 | 2017-05-04 | Method of producing a recombinant protein |
Country Status (14)
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020165874A1 (en) * | 2019-02-15 | 2020-08-20 | Oncosimis Biotech Private Limited | A bacterial expression vector for enhanced protein secretion |
| KR102094480B1 (ko) | 2019-05-27 | 2020-04-01 | (주)엘에스케이화인텍스 | 재사용 가능한 미세 먼지 항균 마스크 |
| CN110551199B (zh) * | 2019-09-26 | 2022-01-25 | 杭州纽龙日尚生物制品有限公司 | 一种提高工程菌分泌表达重组人表皮生长因子的方法 |
| KR20240021234A (ko) | 2021-06-10 | 2024-02-16 | 펠리칸 테크놀로지 홀딩스, 인크. | 재조합 단백질 발현을 위한 박테리아 숙주 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1546342A2 (en) * | 2002-09-20 | 2005-06-29 | Cym1P A/S | Methods for increasing the production of a recombinant polypeptide from a host cell |
| GB0426160D0 (en) * | 2004-11-29 | 2004-12-29 | Era Plantech S L | Production of proteins |
| KR100981356B1 (ko) * | 2006-01-31 | 2010-09-14 | 대한민국 | 신호서열 및 변이된 신호서열로 디자인된 분비증강자에의한 원래 형태의 수용성 재조합 단백질 생산 방법 |
| GB0602173D0 (en) | 2006-02-03 | 2006-03-15 | Avecia Ltd | Expression system |
| WO2009021548A1 (en) * | 2007-08-10 | 2009-02-19 | Wacker Chemie Ag | Expression of full length igg and secretion into the culture medium of prokaryotic cells |
| RU2488635C1 (ru) * | 2012-03-22 | 2013-07-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития | Способ получения рекомбинантного антиангиогенного полипептида |
| US20140244228A1 (en) * | 2012-09-19 | 2014-08-28 | Agency For Science, Technology And Research | Codon optimization of a synthetic gene(s) for protein expression |
-
2017
- 2017-05-04 CN CN201780028031.XA patent/CN109071611B/zh not_active Expired - Fee Related
- 2017-05-04 RU RU2018137305A patent/RU2742006C2/ru active
- 2017-05-04 SG SG11201808976WA patent/SG11201808976WA/en unknown
- 2017-05-04 MX MX2018013562A patent/MX2018013562A/es unknown
- 2017-05-04 WO PCT/EP2017/060654 patent/WO2017191255A1/en not_active Ceased
- 2017-05-04 CA CA3023025A patent/CA3023025A1/en not_active Abandoned
- 2017-05-04 BR BR112018072043-7A patent/BR112018072043A2/pt not_active Application Discontinuation
- 2017-05-04 TW TW106114729A patent/TWI794170B/zh active
- 2017-05-04 JP JP2018558148A patent/JP2019516371A/ja active Pending
- 2017-05-04 AU AU2017260277A patent/AU2017260277A1/en not_active Abandoned
- 2017-05-04 US US16/098,890 patent/US11098312B2/en active Active
- 2017-05-04 KR KR1020187034736A patent/KR102504317B1/ko active Active
- 2017-05-04 EP EP17725188.1A patent/EP3452496A1/en not_active Withdrawn
-
2018
- 2018-10-24 IL IL262544A patent/IL262544A/en unknown
-
2022
- 2022-02-03 JP JP2022015944A patent/JP2022070902A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US20190177734A1 (en) | 2019-06-13 |
| JP2019516371A (ja) | 2019-06-20 |
| SG11201808976WA (en) | 2018-11-29 |
| AU2017260277A1 (en) | 2018-11-22 |
| IL262544A (en) | 2018-12-31 |
| KR102504317B1 (ko) | 2023-02-24 |
| RU2742006C2 (ru) | 2021-02-01 |
| KR20190003694A (ko) | 2019-01-09 |
| BR112018072043A2 (pt) | 2019-02-12 |
| CN109071611B (zh) | 2022-11-01 |
| RU2018137305A (ru) | 2020-06-08 |
| US11098312B2 (en) | 2021-08-24 |
| WO2017191255A1 (en) | 2017-11-09 |
| TW201805298A (zh) | 2018-02-16 |
| TWI794170B (zh) | 2023-03-01 |
| JP2022070902A (ja) | 2022-05-13 |
| MX2018013562A (es) | 2019-03-14 |
| RU2018137305A3 (cg-RX-API-DMAC10.html) | 2020-06-08 |
| CN109071611A (zh) | 2018-12-21 |
| EP3452496A1 (en) | 2019-03-13 |
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| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20220301 |