RU2742006C2 - Способ продуцирования рекомбинантного белка - Google Patents

Способ продуцирования рекомбинантного белка Download PDF

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Publication number
RU2742006C2
RU2742006C2 RU2018137305A RU2018137305A RU2742006C2 RU 2742006 C2 RU2742006 C2 RU 2742006C2 RU 2018137305 A RU2018137305 A RU 2018137305A RU 2018137305 A RU2018137305 A RU 2018137305A RU 2742006 C2 RU2742006 C2 RU 2742006C2
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Russia
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recombinant
protein
expression
signal sequence
recombinant protein
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RU2018137305A
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Russian (ru)
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RU2018137305A (ru
RU2018137305A3 (cg-RX-API-DMAC10.html
Inventor
Робин Александра ЕММИНС
Гэри Брайан ФИНКА
Майкл ХОАР
Алан Питер Льюис
Уилльям Джон Кеннет ЛЬЮИС
Адам Даниель МАКИНОЛИ
Марк Уден
Яннис ВУЛГАРИС
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Глаксосмитклайн Интеллекчуал Проперти Дивелопмент Лимитед
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Publication of RU2018137305A publication Critical patent/RU2018137305A/ru
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
RU2018137305A 2016-05-06 2017-05-04 Способ продуцирования рекомбинантного белка RU2742006C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662332636P 2016-05-06 2016-05-06
US62/332,636 2016-05-06
US201662338045P 2016-05-18 2016-05-18
US62/338,045 2016-05-18
PCT/EP2017/060654 WO2017191255A1 (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Publications (3)

Publication Number Publication Date
RU2018137305A RU2018137305A (ru) 2020-06-08
RU2018137305A3 RU2018137305A3 (cg-RX-API-DMAC10.html) 2020-06-08
RU2742006C2 true RU2742006C2 (ru) 2021-02-01

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
RU2018137305A RU2742006C2 (ru) 2016-05-06 2017-05-04 Способ продуцирования рекомбинантного белка

Country Status (14)

Country Link
US (1) US11098312B2 (cg-RX-API-DMAC10.html)
EP (1) EP3452496A1 (cg-RX-API-DMAC10.html)
JP (2) JP2019516371A (cg-RX-API-DMAC10.html)
KR (1) KR102504317B1 (cg-RX-API-DMAC10.html)
CN (1) CN109071611B (cg-RX-API-DMAC10.html)
AU (1) AU2017260277A1 (cg-RX-API-DMAC10.html)
BR (1) BR112018072043A2 (cg-RX-API-DMAC10.html)
CA (1) CA3023025A1 (cg-RX-API-DMAC10.html)
IL (1) IL262544A (cg-RX-API-DMAC10.html)
MX (1) MX2018013562A (cg-RX-API-DMAC10.html)
RU (1) RU2742006C2 (cg-RX-API-DMAC10.html)
SG (1) SG11201808976WA (cg-RX-API-DMAC10.html)
TW (1) TWI794170B (cg-RX-API-DMAC10.html)
WO (1) WO2017191255A1 (cg-RX-API-DMAC10.html)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020165874A1 (en) * 2019-02-15 2020-08-20 Oncosimis Biotech Private Limited A bacterial expression vector for enhanced protein secretion
KR102094480B1 (ko) 2019-05-27 2020-04-01 (주)엘에스케이화인텍스 재사용 가능한 미세 먼지 항균 마스크
CN110551199B (zh) * 2019-09-26 2022-01-25 杭州纽龙日尚生物制品有限公司 一种提高工程菌分泌表达重组人表皮生长因子的方法
KR20240021234A (ko) 2021-06-10 2024-02-16 펠리칸 테크놀로지 홀딩스, 인크. 재조합 단백질 발현을 위한 박테리아 숙주

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090011995A1 (en) * 2006-01-31 2009-01-08 Sang Jun Lee Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
RU2488635C1 (ru) * 2012-03-22 2013-07-27 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития Способ получения рекомбинантного антиангиогенного полипептида
US20140244228A1 (en) * 2012-09-19 2014-08-28 Agency For Science, Technology And Research Codon optimization of a synthetic gene(s) for protein expression

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1546342A2 (en) * 2002-09-20 2005-06-29 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090011995A1 (en) * 2006-01-31 2009-01-08 Sang Jun Lee Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
RU2488635C1 (ru) * 2012-03-22 2013-07-27 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития Способ получения рекомбинантного антиангиогенного полипептида
US20140244228A1 (en) * 2012-09-19 2014-08-28 Agency For Science, Technology And Research Codon optimization of a synthetic gene(s) for protein expression

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BERRY M. J. et al., Substitution of cysteine for selenocysteine in type I iodothyronine deiodinase reduces the catalytic efficiency of the protein but enhances its translation, Endocrinology, 1992, V. 131, N. 4, p.1848-1852. *
FRANKEL A.E. et al., Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor, Protein Eng., 2000, v.13, n.8, p.575-581. *
ORLANDO M., Modification of proteins and low molecular weight substances with hydroxyethyl starch (HES), Inauguraldissertation, Giesen, 2003, p.166. *
ORLANDO M., Modification of proteins and low molecular weight substances with hydroxyethyl starch (HES), Inauguraldissertation, Giesen, 2003, p.166. PAKULA A.A. et al., Genetic analysis of protein stability and function. Anna. Rev. Genet. 1989, v.23, p.289-310. FRANKEL A.E. et al., Characterization of diphtheria fusion proteins targeted to the human interleukin-3 receptor, Protein Eng., 2000, v.13, n.8, p.575-581. BERRY M. J. et al., Substitution of cysteine for selenocysteine in type I iodothyronine deiodinase reduces the catalytic efficiency of the protein but enhances its translation, Endocrinology, 1992, V. 131, N. 4, p.1848-1852. *
PAKULA A.A. et al., Genetic analysis of protein stability and function. Anna. Rev. Genet. 1989, v.23, p.289-310. *
YIZHOU ZHOU ET AL., Enhancing full-length antibody production by signal peptide engineering, MICROBIAL CELL FACTORIES, 2016, v.15, n.1, p.1-11. *

Also Published As

Publication number Publication date
US20190177734A1 (en) 2019-06-13
JP2019516371A (ja) 2019-06-20
SG11201808976WA (en) 2018-11-29
AU2017260277A1 (en) 2018-11-22
IL262544A (en) 2018-12-31
KR102504317B1 (ko) 2023-02-24
KR20190003694A (ko) 2019-01-09
BR112018072043A2 (pt) 2019-02-12
CN109071611B (zh) 2022-11-01
RU2018137305A (ru) 2020-06-08
US11098312B2 (en) 2021-08-24
CA3023025A1 (en) 2017-11-09
WO2017191255A1 (en) 2017-11-09
TW201805298A (zh) 2018-02-16
TWI794170B (zh) 2023-03-01
JP2022070902A (ja) 2022-05-13
MX2018013562A (es) 2019-03-14
RU2018137305A3 (cg-RX-API-DMAC10.html) 2020-06-08
CN109071611A (zh) 2018-12-21
EP3452496A1 (en) 2019-03-13

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