SG11201808976WA - Method of producing a recombinant protein - Google Patents

Method of producing a recombinant protein

Info

Publication number
SG11201808976WA
SG11201808976WA SG11201808976WA SG11201808976WA SG11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA SG 11201808976W A SG11201808976W A SG 11201808976WA
Authority
SG
Singapore
Prior art keywords
glaxosmithkline
road
international
hertfordshire
recombinant protein
Prior art date
Application number
SG11201808976WA
Inventor
Robyn Alexandra Emmins
Gary Brian Finka
Michael Hoare
Alan Peter Lewis
William John Kenneth Lewis
Adam Daniel Mcinally
Mark Uden
Ioannis Voulgaris
Original Assignee
Glaxosmithkline Ip Dev Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxosmithkline Ip Dev Ltd filed Critical Glaxosmithkline Ip Dev Ltd
Publication of SG11201808976WA publication Critical patent/SG11201808976WA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111011111111101 IIIIIIIIIIIIIIIIIII1011101111111101111111110111110111011111 Organization International Bureau (10) International Publication Number (43) International Publication Date .....0\"\"\" WO 2017/191255 Al 09 November 2017 (09.11.2017) W I P0 I P C T — (51) (21) (22) (25) Filing Language: (26) (30) (71) (72) International Patent Classification: C07K 14/245 (2006.01) C12N International Application Number: International Filing Date: 04 Publication Language: Priority Data: 62/332,636 06 May 2016 (06.05.2016) 62/338,045 18 May 2016 (18.05.2016) 15/70 (2006.01) London Peter; PCT/EP2017/060654 Hertfordshire nage May 2017 (04.05.2017) Daniel; UDEN, English English Ioannis; sity US London US (74) Agent: INTELLECTUAL Patents LIMITED [GB/GB]; sex TW8 9GS (GB). Middlesex TW8 9GS (81) Designated kind GlaxoSmithK- AO, Hertfordshire SG1 CA, Gun- DZ, SG1 2NY (GB). HR, HOARE, Kenneth; Stevenage neering, Department of Biochemical Engineering, Univer- Michael; 32 Cherry Tree Road, East Finchley, Greater London N2 9QL (GB). LEWIS, Alan GlaxoSmithKline, Gunnels Wood Road, Stevenage SG1 2NY (GB). LEWIS, William John GlaxoSmithKline, Gunnels Wood Road, Steve- Hertfordshire SG1 2NY (GB). MCINALLY, Adam 1/2 9 Yorkhill Parade, Glasgow G3 8SW (GB). Mark; GlaxoSmithKline, Gunnels Wood Road, Hertfordshire SG1 2NY (GB). VOULGARIS, c/o The Advanced Centre for Biochemical Engi- College London, Bernard Katz Building, Gordon Street, WC1H OAH (GB). HARRISON, Anna; GlaxoSmithKline, Global Applicant: GLAXOSMITHKLINE PROPERTY DEVELOPMENT 980 Great West Road, Brentford (GB). Inventors: EMMINS, Robyn Alexandra; line, Gunnels Wood Road, Stevenage 2NY (GB). FINKA, Gary Brian; GlaxoSmithKline, nels Wood Road, Stevenage Hertfordshire CN925.1, 980 Great West Road, Brentford Middle- States (unless otherwise indicated, for every of national protection available): AE, AG, AL, AM, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, = Title: METHOD OF PRODUCING A 0.2 : The present invention relates comprising the signal sequence, a sequence can be used to provide a method of the recombinant protein. (54) protein N expression A RECOMBINANT PROTEIN 044 0.11 0.15 Figure 5 D.07 0.04 9.16 0.13 = = = — _ — = — = = = = = = = .V. . .1.:1 . to a recombinant signal sequence recombinant protein and methods of producing the recombinant protein. The recombinant for controlling the viscosity of the fermentation, and/or controlling basal (pre-induction) -,, . derived from E. Coli. The invention also relates to a fusion Il in in ei 1-1 cT 1-1 ----. (57) IN 0 signal C [Continued on next page] WO 2017/191255 Al MIDEDIMOMOIDEIROIDEMOMOHHOH1MODEVOIMIE KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: — with international search report (Art. 21(3)) — with sequence listing part of description (Rule 5.2(a))
SG11201808976WA 2016-05-06 2017-05-04 Method of producing a recombinant protein SG11201808976WA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662332636P 2016-05-06 2016-05-06
US201662338045P 2016-05-18 2016-05-18
PCT/EP2017/060654 WO2017191255A1 (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Publications (1)

Publication Number Publication Date
SG11201808976WA true SG11201808976WA (en) 2018-11-29

Family

ID=58765814

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201808976WA SG11201808976WA (en) 2016-05-06 2017-05-04 Method of producing a recombinant protein

Country Status (14)

Country Link
US (1) US11098312B2 (en)
EP (1) EP3452496A1 (en)
JP (2) JP2019516371A (en)
KR (1) KR102504317B1 (en)
CN (1) CN109071611B (en)
AU (1) AU2017260277A1 (en)
BR (1) BR112018072043A2 (en)
CA (1) CA3023025A1 (en)
IL (1) IL262544A (en)
MX (1) MX2018013562A (en)
RU (1) RU2742006C2 (en)
SG (1) SG11201808976WA (en)
TW (1) TWI794170B (en)
WO (1) WO2017191255A1 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102094480B1 (en) 2019-05-27 2020-04-01 (주)엘에스케이화인텍스 Reusable antibacterial mask for fine dust
CN110551199B (en) * 2019-09-26 2022-01-25 杭州纽龙日尚生物制品有限公司 Method for improving secretion expression of recombinant human epidermal growth factor by engineering bacteria
EP4352219A1 (en) * 2021-06-10 2024-04-17 Pelican Technology Holdings, Inc. Bacterial hosts for recombinant protein expression

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1701119A (en) * 2002-09-20 2005-11-23 Cym1P有限公司 Methods for increasing the production of a recombinant polypeptide from a host cell
GB0426160D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Production of proteins
EP1981979A4 (en) * 2006-01-31 2009-07-29 Republic Of Korea Republic Of Production of a soluble native form of recombinant protein by the signal sequence and secretional enhancer
GB0602173D0 (en) 2006-02-03 2006-03-15 Avecia Ltd Expression system
WO2009021548A1 (en) * 2007-08-10 2009-02-19 Wacker Chemie Ag Expression of full length igg and secretion into the culture medium of prokaryotic cells
RU2488635C1 (en) 2012-03-22 2013-07-27 Государственное бюджетное образовательное учреждение высшего профессионального образования Первый Московский государственный медицинский университет им. И.М. Сеченова Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО Первый МГМУ им. И.М. Сеченова Минздравсоцразвития Method to produce recombinant anti-angiogenic polypeptide
SG10201602115PA (en) 2012-09-19 2016-05-30 Univ Singapore Codon optimization of a synthetic gene(s) for protein expression

Also Published As

Publication number Publication date
WO2017191255A1 (en) 2017-11-09
RU2018137305A (en) 2020-06-08
KR20190003694A (en) 2019-01-09
US20190177734A1 (en) 2019-06-13
CN109071611A (en) 2018-12-21
EP3452496A1 (en) 2019-03-13
TW201805298A (en) 2018-02-16
TWI794170B (en) 2023-03-01
RU2018137305A3 (en) 2020-06-08
JP2022070902A (en) 2022-05-13
JP2019516371A (en) 2019-06-20
AU2017260277A1 (en) 2018-11-22
RU2742006C2 (en) 2021-02-01
BR112018072043A2 (en) 2019-02-12
IL262544A (en) 2018-12-31
US11098312B2 (en) 2021-08-24
CA3023025A1 (en) 2017-11-09
CN109071611B (en) 2022-11-01
MX2018013562A (en) 2019-03-14
KR102504317B1 (en) 2023-02-24

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