CA3004310A1 - Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells - Google Patents

Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells Download PDF

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Publication number
CA3004310A1
CA3004310A1 CA3004310A CA3004310A CA3004310A1 CA 3004310 A1 CA3004310 A1 CA 3004310A1 CA 3004310 A CA3004310 A CA 3004310A CA 3004310 A CA3004310 A CA 3004310A CA 3004310 A1 CA3004310 A1 CA 3004310A1
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nucleic acid
barcode
sequence
barcodes
sample
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French (fr)
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Gary WITHEY
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Atreca Inc
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Atreca Inc
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1065Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q2523/00Reactions characterised by treatment of reaction samples
    • C12Q2523/30Characterised by physical treatment
    • C12Q2523/319Photocleavage, photolysis, photoactivation
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/173Modifications characterised by incorporating a polynucleotide run, e.g. polyAs, polyTs
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    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
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    • C12Q2525/191Modifications characterised by incorporating an adaptor
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    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/149Particles, e.g. beads
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/159Microreactors, e.g. emulsion PCR or sequencing, droplet PCR, microcapsules, i.e. non-liquid containers with a range of different permeability's for different reaction components
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
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    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/185Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
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    • C12Q2565/00Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50Detection characterised by immobilisation to a surface
    • C12Q2565/514Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Chemical & Material Sciences (AREA)
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  • Health & Medical Sciences (AREA)
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  • Wood Science & Technology (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Toxicology (AREA)
CA3004310A 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells Pending CA3004310A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562250900P 2015-11-04 2015-11-04
US62/250,900 2015-11-04
PCT/US2016/060576 WO2017079593A1 (en) 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells

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CA3004310A1 true CA3004310A1 (en) 2017-05-11

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CA3004310A Pending CA3004310A1 (en) 2015-11-04 2016-11-04 Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells

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US (2) US11098304B2 (OSRAM)
EP (1) EP3371309B1 (OSRAM)
JP (1) JP7064439B2 (OSRAM)
KR (1) KR20180097536A (OSRAM)
CN (1) CN108473984A (OSRAM)
AU (1) AU2016348439B2 (OSRAM)
CA (1) CA3004310A1 (OSRAM)
SG (1) SG11201803646YA (OSRAM)
WO (1) WO2017079593A1 (OSRAM)

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