CA2995872C - Recombinant clostridium bacterium and uses thereof in acetone production - Google Patents

Recombinant clostridium bacterium and uses thereof in acetone production Download PDF

Info

Publication number
CA2995872C
CA2995872C CA2995872A CA2995872A CA2995872C CA 2995872 C CA2995872 C CA 2995872C CA 2995872 A CA2995872 A CA 2995872A CA 2995872 A CA2995872 A CA 2995872A CA 2995872 C CA2995872 C CA 2995872C
Authority
CA
Canada
Prior art keywords
seq
acetone
microorganism
decarboxylase
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2995872A
Other languages
English (en)
French (fr)
Other versions
CA2995872A1 (en
Inventor
Sean Simpson
Michael Koepke
Fungmin Liew
Wendy Yiting Chen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lanzatech NZ Inc
Original Assignee
Lanzatech New Zealand Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lanzatech New Zealand Ltd filed Critical Lanzatech New Zealand Ltd
Publication of CA2995872A1 publication Critical patent/CA2995872A1/en
Application granted granted Critical
Publication of CA2995872C publication Critical patent/CA2995872C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/13—Transferases (2.) transferring sulfur containing groups (2.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C12P7/28—Acetone-containing products
    • C12P7/30—Acetone-containing products produced from substrate containing inorganic compounds other than water
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01001—Alcohol dehydrogenase (1.1.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01002—Alcohol dehydrogenase (NADP+) (1.1.1.2), i.e. aldehyde reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01009—Acetyl-CoA C-acetyltransferase (2.3.1.9)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01004—Acetoacetate decarboxylase (4.1.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01074—Indolepyruvate decarboxylase (4.1.1.74)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/10—Process efficiency
    • Y02P20/133—Renewable energy sources, e.g. sunlight

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2995872A 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in acetone production Active CA2995872C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161446832P 2011-02-25 2011-02-25
US61/446,832 2011-02-25
CA2825267A CA2825267C (en) 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in isopropanol production

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2825267A Division CA2825267C (en) 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in isopropanol production

Publications (2)

Publication Number Publication Date
CA2995872A1 CA2995872A1 (en) 2012-08-30
CA2995872C true CA2995872C (en) 2019-09-17

Family

ID=46721376

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2995872A Active CA2995872C (en) 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in acetone production
CA2825267A Active CA2825267C (en) 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in isopropanol production

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2825267A Active CA2825267C (en) 2011-02-25 2012-02-24 Recombinant clostridium bacterium and uses thereof in isopropanol production

Country Status (13)

Country Link
EP (2) EP2678432B1 (cg-RX-API-DMAC7.html)
JP (1) JP6199747B2 (cg-RX-API-DMAC7.html)
KR (2) KR102015672B1 (cg-RX-API-DMAC7.html)
CN (2) CN103502435B (cg-RX-API-DMAC7.html)
AU (1) AU2012221176B2 (cg-RX-API-DMAC7.html)
BR (1) BR112013021524A2 (cg-RX-API-DMAC7.html)
CA (2) CA2995872C (cg-RX-API-DMAC7.html)
DK (1) DK2678432T3 (cg-RX-API-DMAC7.html)
ES (1) ES2702621T3 (cg-RX-API-DMAC7.html)
MY (2) MY168208A (cg-RX-API-DMAC7.html)
PL (1) PL2678432T3 (cg-RX-API-DMAC7.html)
TW (1) TWI541348B (cg-RX-API-DMAC7.html)
WO (1) WO2012115527A2 (cg-RX-API-DMAC7.html)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102079274B1 (ko) * 2012-04-05 2020-02-20 란자테크 뉴질랜드 리미티드 효소 변경된 대사 산물의 활성
US20130316364A1 (en) 2012-05-23 2013-11-28 Lanzatech New Zealand Limited Selection Method and Recombinant Microorganisms and uses Therefor
KR102098849B1 (ko) 2012-05-30 2020-04-09 란자테크 뉴질랜드 리미티드 재조합 미생물 및 이의 용도
US20130323820A1 (en) 2012-06-01 2013-12-05 Lanzatech New Zealand Limited Recombinant microorganisms and uses therefor
KR102079275B1 (ko) 2012-06-08 2020-02-19 란자테크 뉴질랜드 리미티드 재조합 미생물 및 그에 대한 용도
US9347076B2 (en) 2012-06-21 2016-05-24 Lanzatech New Zealand Limited Recombinant microorganisms that make biodiesel
EA201592169A1 (ru) * 2013-06-05 2016-04-29 Ланцатек Нью Зилэнд Лимитед Рекомбинантные микроорганизмы, приводящие к увеличению потока через путь ферментации
CN105722990B (zh) * 2013-09-22 2020-10-20 朗泽科技新西兰有限公司 发酵工艺
CN105874057A (zh) * 2013-12-03 2016-08-17 朗泽科技新西兰有限公司 用于酮生产的微生物和方法
SG11201605775UA (en) 2014-01-28 2016-08-30 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
ES2946180T3 (es) 2014-10-22 2023-07-13 Lanzatech Nz Inc Procesos de biorreactores de múltiples etapas
JP2017531441A (ja) 2014-10-22 2017-10-26 ランザテク・ニュージーランド・リミテッド ガス試験装置及び方法
AU2015360786B2 (en) 2014-12-08 2021-10-21 Lanzatech Nz, Inc. Recombinant microorganisms exhibiting increased flux through a fermentation pathway
EP3262153A4 (en) 2015-02-23 2018-07-04 Lanzatech New Zealand Limited Recombinant acetogenic bacterium for the conversion of methane to products
US9738875B2 (en) * 2015-10-13 2017-08-22 Lanzatech New Zealand Limited Genetically engineered bacterium comprising energy-generating fermentation pathway
EP3384005B1 (en) 2015-12-03 2022-02-02 LanzaTech NZ, Inc. Arginine as sole nitrogen source for c1-fixing microorganism
US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
EP4234707A3 (en) 2016-02-01 2024-06-05 LanzaTech NZ, Inc. Integrated fermentation and electrolysis process
KR20180110144A (ko) 2016-02-26 2018-10-08 란자테크 뉴질랜드 리미티드 C1-고정 박테리아에 대한 crispr/cas 시스템
EP3464559A4 (en) 2016-05-14 2020-02-12 Lanzatech, Inc. MODIFIED ALDEHYDE / FERREDOXIN OXYDOREDUCTASE MICROORGANISM AND RELATED METHODS
CN117551705A (zh) 2017-09-08 2024-02-13 朗泽科技有限公司 用于使用富氢气的含有c1的底物生产代谢物的方法和系统
MY202145A (en) 2018-02-12 2024-04-05 Lanzatech Inc Process for improving carbon conversion efficiency
EP3781668B1 (en) 2018-04-20 2023-10-04 Lanzatech, Inc. Fermentation process for producing ethanol
US11097967B2 (en) 2018-11-19 2021-08-24 Lanzatech, Inc. Integration of fermentation and gasification
JP7224481B2 (ja) 2019-02-08 2023-02-17 ランザテク，インコーポレイテッド 近接沸騰生成物を回収するためのプロセス
CA3127429A1 (en) 2019-03-20 2020-09-24 Global Bioenergies Improved means and methods for producing isobutene from acetyl-coa
CN112955560A (zh) 2019-07-11 2021-06-11 朗泽科技有限公司 优化气体利用的方法
CN111057686B (zh) * 2019-12-23 2021-05-04 浙江大学 一种醇脱氢酶突变体及应用
US11932818B2 (en) 2020-03-16 2024-03-19 Lanzatech, Inc. Tail gas of gas fermentation to dry gasification feedstock
BR112022018609A2 (pt) 2020-03-18 2022-11-08 Lanzatech Inc Microrganismo capaz de produzir 2-feniletanol, e, método de produção de 2-feniletanol
CA3224707A1 (en) * 2020-06-06 2021-12-09 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
WO2022066997A1 (en) * 2020-09-25 2022-03-31 Lanzatech, Inc. Recombinant microorganisms and uses therefor
WO2022170191A1 (en) 2021-02-08 2022-08-11 Lanzatech, Inc. Recombinant microorganisms and uses therefor
BR112023020761A2 (pt) 2021-04-09 2023-12-12 Lanzatech Inc Processo para fornecer uma matéria-prima, e, aparelho para fornecer uma matéria-prima de fermentação gasosa
CN114561416B (zh) * 2022-04-27 2022-07-15 深圳中科翎碳生物科技有限公司 利用电能固定co2及合成异丙醇的工程菌及构建方法
WO2024036187A1 (en) 2022-08-10 2024-02-15 Lanzatech, Inc. Carbon sequestration in soils with production of chemical products
WO2024231219A1 (en) 2023-05-11 2024-11-14 Evonik Operations Gmbh Biotechnological production of acetone
CN117625571B (zh) * 2023-12-05 2025-06-10 迪嘉药业集团股份有限公司 一种改进的酮还原酶及其在依折麦布制备中的应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173429A (en) 1990-11-09 1992-12-22 The Board Of Trustees Of The University Of Arkansas Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism
US5593886A (en) 1992-10-30 1997-01-14 Gaddy; James L. Clostridium stain which produces acetic acid from waste gases
UA72220C2 (uk) 1998-09-08 2005-02-15 Байоенджініерінг Рісорсиз, Інк. Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання
US7285402B2 (en) 2000-07-25 2007-10-23 Emmaus Foundation, Inc. Methods for increasing the production of ethanol from microbial fermentation
ZA200803755B (en) * 2005-10-26 2009-12-30 Du Pont Fermentive production of four carbon alcohols
NZ546496A (en) * 2006-04-07 2008-09-26 Lanzatech New Zealand Ltd Gas treatment process
US7704723B2 (en) 2006-08-31 2010-04-27 The Board Of Regents For Oklahoma State University Isolation and characterization of novel clostridial species
JP2011500031A (ja) * 2007-10-12 2011-01-06 ザ レジェンツ オブ ザ ユニヴァースティ オブ カリフォルニア イソプロパノールを生産するように操作された微生物
BRPI0820556B1 (pt) 2007-11-13 2016-03-22 Lanzatech New Zealand Ltd bactéria e métodos para uso das mesmas
NZ587563A (en) * 2008-03-12 2012-07-27 Lanzatech New Zealand Ltd Microbial alcohol production process
WO2009131040A1 (ja) * 2008-04-25 2009-10-29 財団法人地球環境産業技術研究機構 イソプロパノール生産能を有するコリネ型細菌の形質転換体
KR20110097951A (ko) * 2008-12-16 2011-08-31 게노마티카 인코포레이티드 합성가스와 다른 탄소원을 유용 제품으로 전환시키기 위한 미생물 및 방법
DE102009002583A1 (de) 2009-04-23 2010-10-28 Evonik Degussa Gmbh Zellen und Verfahren zur Herstellung von Aceton
CN102666862A (zh) * 2009-08-21 2012-09-12 马斯科马公司 在联合生物加工生物体中生产丙醇、醇和多元醇
US8143037B2 (en) 2010-03-19 2012-03-27 Coskata, Inc. Ethanologenic Clostridium species, Clostridium coskatii

Also Published As

Publication number Publication date
EP3401405A1 (en) 2018-11-14
WO2012115527A2 (en) 2012-08-30
TWI541348B (zh) 2016-07-11
EP2678432B1 (en) 2018-08-01
CN103502435A (zh) 2014-01-08
CA2825267C (en) 2018-04-03
KR20140012106A (ko) 2014-01-29
CN108486169B (zh) 2022-08-12
TW201309795A (zh) 2013-03-01
ES2702621T3 (es) 2019-03-04
DK2678432T3 (en) 2018-11-26
CN108486169A (zh) 2018-09-04
PL2678432T4 (pl) 2019-05-31
CA2825267A1 (en) 2012-08-30
CN103502435B (zh) 2018-05-18
KR102121886B1 (ko) 2020-06-15
NZ614459A (en) 2015-09-25
KR102015672B1 (ko) 2019-08-29
BR112013021524A2 (pt) 2017-06-13
MY168208A (en) 2018-10-15
CA2995872A1 (en) 2012-08-30
WO2012115527A3 (en) 2013-01-10
KR20190097292A (ko) 2019-08-20
AU2012221176B2 (en) 2015-03-19
EP2678432A4 (en) 2016-06-22
AU2012221176A1 (en) 2013-05-09
JP6199747B2 (ja) 2017-09-20
MY193665A (en) 2022-10-24
PL2678432T3 (pl) 2019-05-31
JP2014506478A (ja) 2014-03-17
EP2678432A2 (en) 2014-01-01

Similar Documents

Publication Publication Date Title
CA2995872C (en) Recombinant clostridium bacterium and uses thereof in acetone production
US9834795B2 (en) Recombinant microorganisms and uses therefor
US9365868B2 (en) Fermentation process for producing isopropanol using a recombinant microorganism
AU2011318676B2 (en) Production of butanol from carbon monoxide by a recombinant microorganism
JP6445970B2 (ja) 組換え微生物およびその使用
CA2914003C (en) Recombinant microorganisms exhibiting increased flux through a fermentation pathway
JP6407141B2 (ja) 代謝物活性が改変された酵素
NZ614459B2 (en) Recombinant microorganisms and uses therefor

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180221