CA2966442C - Sequencing from multiple primers to increase data rate and density - Google Patents
Sequencing from multiple primers to increase data rate and density Download PDFInfo
- Publication number
- CA2966442C CA2966442C CA2966442A CA2966442A CA2966442C CA 2966442 C CA2966442 C CA 2966442C CA 2966442 A CA2966442 A CA 2966442A CA 2966442 A CA2966442 A CA 2966442A CA 2966442 C CA2966442 C CA 2966442C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- primers
- nucleotide
- extension
- sequencing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30072—Microarray; Biochip, DNA array; Well plate
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Theoretical Computer Science (AREA)
- General Physics & Mathematics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Quality & Reliability (AREA)
- Radiology & Medical Imaging (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Medical Informatics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Chemical Kinetics & Catalysis (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1419731.3 | 2014-11-05 | ||
| GBGB1419731.3A GB201419731D0 (en) | 2014-11-05 | 2014-11-05 | Sequencing from multiple primers to increase data rate and density |
| PCT/GB2015/053324 WO2016071689A1 (en) | 2014-11-05 | 2015-11-04 | Sequencing from multiple primers to increase data rate and density |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2966442A1 CA2966442A1 (en) | 2016-05-12 |
| CA2966442C true CA2966442C (en) | 2024-03-12 |
Family
ID=52118764
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2966442A Active CA2966442C (en) | 2014-11-05 | 2015-11-04 | Sequencing from multiple primers to increase data rate and density |
Country Status (11)
| Country | Link |
|---|---|
| US (3) | US20170298430A1 (OSRAM) |
| EP (2) | EP3974538A1 (OSRAM) |
| JP (1) | JP6789935B2 (OSRAM) |
| CN (2) | CN113564238B (OSRAM) |
| AU (2) | AU2015341568B2 (OSRAM) |
| CA (1) | CA2966442C (OSRAM) |
| DK (1) | DK3215638T3 (OSRAM) |
| ES (1) | ES2889875T3 (OSRAM) |
| GB (1) | GB201419731D0 (OSRAM) |
| SG (1) | SG11201703629XA (OSRAM) |
| WO (1) | WO2016071689A1 (OSRAM) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10294514B2 (en) | 2016-04-29 | 2019-05-21 | Omniome, Inc. | Sequencing method employing ternary complex destabilization to identify cognate nucleotides |
| WO2018034780A1 (en) | 2016-08-15 | 2018-02-22 | Omniome, Inc. | Sequencing method for rapid identification and processing of cognate nucleotide pairs |
| EP3519586B1 (en) * | 2016-09-28 | 2024-03-13 | Life Technologies Corporation | Methods for sequencing nucleic acids using termination chemistry |
| CA3048415C (en) | 2016-12-30 | 2023-02-28 | Omniome, Inc. | Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides |
| DE102017218849A1 (de) * | 2017-10-23 | 2019-04-25 | Robert Bosch Gmbh | Reaktionsträger für eine mikrofluidische Vorrichtung und Verfahren zur Bestimmung einer Nukleotidsequenz |
| CN113249455A (zh) * | 2020-02-12 | 2021-08-13 | 赛纳生物科技(北京)有限公司 | 一种基因测序中获得背景信号的方法 |
| WO2023114392A1 (en) * | 2021-12-16 | 2023-06-22 | Ultima Genomics, Inc. | Systems and methods for sequencing with multi-priming |
| WO2023175029A1 (en) * | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Concurrent sequencing of hetero n-mer polynucleotides |
| WO2023175037A2 (en) * | 2022-03-15 | 2023-09-21 | Illumina, Inc. | Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection |
| CN115232867B (zh) * | 2022-07-21 | 2024-01-30 | 深圳赛陆医疗科技有限公司 | 一种快速的二代基因测序方法 |
| WO2024124379A1 (zh) * | 2022-12-12 | 2024-06-20 | 深圳华大智造科技股份有限公司 | 一种多模板核酸同步测序的方法及其应用 |
| CN117721191B (zh) * | 2024-02-07 | 2024-05-10 | 深圳赛陆医疗科技有限公司 | 基因测序方法、测序装置、可读存储介质和基因测序系统 |
Family Cites Families (58)
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|---|---|---|---|---|
| CA1323293C (en) | 1987-12-11 | 1993-10-19 | Keith C. Backman | Assay using template-dependent nucleic acid probe reorganization |
| CA1341584C (en) | 1988-04-06 | 2008-11-18 | Bruce Wallace | Method of amplifying and detecting nucleic acid sequences |
| WO1989009835A1 (en) | 1988-04-08 | 1989-10-19 | The Salk Institute For Biological Studies | Ligase-based amplification method |
| WO1989012696A1 (en) | 1988-06-24 | 1989-12-28 | Amgen Inc. | Method and reagents for detecting nucleic acid sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| WO1990001069A1 (en) | 1988-07-20 | 1990-02-08 | Segev Diagnostics, Inc. | Process for amplifying and detecting nucleic acid sequences |
| US5185243A (en) | 1988-08-25 | 1993-02-09 | Syntex (U.S.A.) Inc. | Method for detection of specific nucleic acid sequences |
| WO1991006678A1 (en) | 1989-10-26 | 1991-05-16 | Sri International | Dna sequencing |
| EP0439182B1 (en) | 1990-01-26 | 1996-04-24 | Abbott Laboratories | Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| CA2182517C (en) | 1994-02-07 | 2001-08-21 | Theo Nikiforov | Ligase/polymerase-mediated primer extension of single nucleotide polymorphisms and its use in genetic analysis |
| CA2185239C (en) | 1994-03-16 | 2002-12-17 | Nanibhushan Dattagupta | Isothermal strand displacement nucleic acid amplification |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| DE19515552A1 (de) * | 1995-04-27 | 1996-10-31 | Europ Lab Molekularbiolog | Simultane Sequenzierung von Nukleinsäuren |
| GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
| GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
| WO1998044151A1 (en) | 1997-04-01 | 1998-10-08 | Glaxo Group Limited | Method of nucleic acid amplification |
| US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
| AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
| US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
| AU1075701A (en) * | 1999-10-08 | 2001-04-23 | Protogene Laboratories, Inc. | Method and apparatus for performing large numbers of reactions using array assembly |
| US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
| US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
| US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
| EP1368460B1 (en) | 2000-07-07 | 2007-10-31 | Visigen Biotechnologies, Inc. | Real-time sequence determination |
| US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
| EP1472335A4 (en) * | 2001-11-08 | 2005-12-28 | Univ Johns Hopkins | METHODS AND SYSTEMS FOR SEQUENCING NUCLEIC ACIDS |
| US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
| WO2004018497A2 (en) | 2002-08-23 | 2004-03-04 | Solexa Limited | Modified nucleotides for polynucleotide sequencing |
| EP1636337A4 (en) | 2003-06-20 | 2007-07-04 | Illumina Inc | METHODS AND COMPOSITIONS USEFUL FOR THE AMPLIFICATION AND GENOTYPING OF THE GENOME |
| GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
| US7169560B2 (en) * | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| ES2949821T3 (es) | 2004-01-07 | 2023-10-03 | Illumina Cambridge Ltd | Matrices moleculares |
| AU2005296200B2 (en) | 2004-09-17 | 2011-07-14 | Pacific Biosciences Of California, Inc. | Apparatus and method for analysis of molecules |
| WO2006064199A1 (en) | 2004-12-13 | 2006-06-22 | Solexa Limited | Improved method of nucleotide detection |
| JP4990886B2 (ja) | 2005-05-10 | 2012-08-01 | ソレックサ リミテッド | 改良ポリメラーゼ |
| GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
| US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
| JP5122555B2 (ja) | 2006-03-31 | 2013-01-16 | ソレクサ・インコーポレイテッド | 合成解析による配列決定システムおよび装置 |
| US7754429B2 (en) | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| EP2089517A4 (en) | 2006-10-23 | 2010-10-20 | Pacific Biosciences California | POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING |
| US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
| US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
| EP3285067B1 (en) | 2006-12-14 | 2022-06-22 | Life Technologies Corporation | Apparatus for measuring analytes using fet arrays |
| EP2121983A2 (en) * | 2007-02-02 | 2009-11-25 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| DK2725107T3 (da) * | 2007-10-19 | 2019-01-02 | Univ Columbia | DNA-sekventering med ikke-fluorescerende nukleotidreversible terminatorer og ddNTP'er modificeret med spaltbart mærke og nukleinsyre omfattende inosin med reversible terminatorer |
| US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
| US20110195410A1 (en) * | 2009-12-07 | 2011-08-11 | Gene Check, Inc. | Compositions and methods related to solid phase sequence detection and genotyping |
| CN102140534A (zh) * | 2010-12-15 | 2011-08-03 | 深圳华大基因科技有限公司 | 一种hbv基因的核苷酸突变位点的检测方法 |
| US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
| US11130992B2 (en) * | 2011-03-31 | 2021-09-28 | Dana-Farber Cancer Institute, Inc. | Methods and compositions to enable multiplex COLD-PCR |
| US20120252682A1 (en) * | 2011-04-01 | 2012-10-04 | Maples Corporate Services Limited | Methods and systems for sequencing nucleic acids |
| PL3290528T3 (pl) | 2011-09-23 | 2020-03-31 | Illumina, Inc. | Sposoby i kompozycje do sekwencjonowania kwasów nukleinowych |
| IN2014DN07992A (OSRAM) | 2012-04-03 | 2015-05-01 | Illumina Inc | |
| WO2015002789A1 (en) * | 2013-07-03 | 2015-01-08 | Illumina, Inc. | Sequencing by orthogonal synthesis |
-
2014
- 2014-11-05 GB GBGB1419731.3A patent/GB201419731D0/en not_active Ceased
-
2015
- 2015-11-04 DK DK15794241.8T patent/DK3215638T3/da active
- 2015-11-04 EP EP21190518.7A patent/EP3974538A1/en active Pending
- 2015-11-04 EP EP15794241.8A patent/EP3215638B1/en active Active
- 2015-11-04 ES ES15794241T patent/ES2889875T3/es active Active
- 2015-11-04 SG SG11201703629XA patent/SG11201703629XA/en unknown
- 2015-11-04 US US15/510,191 patent/US20170298430A1/en not_active Abandoned
- 2015-11-04 CN CN202110829488.9A patent/CN113564238B/zh active Active
- 2015-11-04 CA CA2966442A patent/CA2966442C/en active Active
- 2015-11-04 WO PCT/GB2015/053324 patent/WO2016071689A1/en not_active Ceased
- 2015-11-04 JP JP2017524043A patent/JP6789935B2/ja active Active
- 2015-11-04 CN CN201580069430.1A patent/CN107257862B/zh active Active
- 2015-11-04 AU AU2015341568A patent/AU2015341568B2/en active Active
-
2020
- 2020-08-03 US US16/983,947 patent/US11555218B2/en active Active
-
2021
- 2021-12-15 AU AU2021286342A patent/AU2021286342B2/en active Active
-
2023
- 2023-01-12 US US18/153,946 patent/US20230160006A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN107257862B (zh) | 2021-08-03 |
| JP6789935B2 (ja) | 2020-11-25 |
| EP3974538A1 (en) | 2022-03-30 |
| CN113564238B (zh) | 2025-08-05 |
| SG11201703629XA (en) | 2017-06-29 |
| AU2015341568B2 (en) | 2021-09-16 |
| CN107257862A (zh) | 2017-10-17 |
| AU2021286342A1 (en) | 2022-01-20 |
| EP3215638A1 (en) | 2017-09-13 |
| GB201419731D0 (en) | 2014-12-17 |
| WO2016071689A1 (en) | 2016-05-12 |
| DK3215638T3 (da) | 2021-10-18 |
| AU2021286342B2 (en) | 2024-05-30 |
| US20200392573A1 (en) | 2020-12-17 |
| HK1243465A1 (en) | 2018-07-13 |
| EP3215638B1 (en) | 2021-08-11 |
| CA2966442A1 (en) | 2016-05-12 |
| AU2015341568A1 (en) | 2017-05-25 |
| JP2017533709A (ja) | 2017-11-16 |
| US20170298430A1 (en) | 2017-10-19 |
| US20230160006A1 (en) | 2023-05-25 |
| ES2889875T3 (es) | 2022-01-14 |
| CN113564238A (zh) | 2021-10-29 |
| US11555218B2 (en) | 2023-01-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20201030 |
|
| EEER | Examination request |
Effective date: 20201030 |
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| EEER | Examination request |
Effective date: 20201030 |
|
| EEER | Examination request |
Effective date: 20201030 |
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| EEER | Examination request |
Effective date: 20201030 |
|
| EEER | Examination request |
Effective date: 20201030 |
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| EEER | Examination request |
Effective date: 20201030 |
|
| EEER | Examination request |
Effective date: 20201030 |