CA2959915C - Expression system - Google Patents

Expression system Download PDF

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Publication number
CA2959915C
CA2959915C CA2959915A CA2959915A CA2959915C CA 2959915 C CA2959915 C CA 2959915C CA 2959915 A CA2959915 A CA 2959915A CA 2959915 A CA2959915 A CA 2959915A CA 2959915 C CA2959915 C CA 2959915C
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CA
Canada
Prior art keywords
promoter
sequence
plasmid
protein
expression system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2959915A
Other languages
English (en)
French (fr)
Other versions
CA2959915A1 (en
Inventor
Ian John Hodgson
Christopher David John Lennon
Bhupendra Vallabh Kara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Fujifilm Diosynth Biotechnologies UK Ltd
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Filing date
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Publication of CA2959915A1 publication Critical patent/CA2959915A1/en
Application granted granted Critical
Publication of CA2959915C publication Critical patent/CA2959915C/en
Active legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA2959915A 2006-02-03 2007-02-01 Expression system Active CA2959915C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0602173.7A GB0602173D0 (en) 2006-02-03 2006-02-03 Expression system
GB0602173.7 2006-02-03
CA2637818A CA2637818C (en) 2006-02-03 2007-02-01 Expression system

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2637818A Division CA2637818C (en) 2006-02-03 2007-02-01 Expression system

Publications (2)

Publication Number Publication Date
CA2959915A1 CA2959915A1 (en) 2007-08-09
CA2959915C true CA2959915C (en) 2022-11-15

Family

ID=36100973

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2959915A Active CA2959915C (en) 2006-02-03 2007-02-01 Expression system
CA2637818A Active CA2637818C (en) 2006-02-03 2007-02-01 Expression system

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2637818A Active CA2637818C (en) 2006-02-03 2007-02-01 Expression system

Country Status (15)

Country Link
US (4) US8530188B2 (cg-RX-API-DMAC7.html)
EP (6) EP1984506B1 (cg-RX-API-DMAC7.html)
JP (5) JP5221385B2 (cg-RX-API-DMAC7.html)
KR (3) KR101624345B1 (cg-RX-API-DMAC7.html)
CN (3) CN102690834B (cg-RX-API-DMAC7.html)
CA (2) CA2959915C (cg-RX-API-DMAC7.html)
DK (4) DK2695943T3 (cg-RX-API-DMAC7.html)
ES (3) ES2569075T3 (cg-RX-API-DMAC7.html)
GB (1) GB0602173D0 (cg-RX-API-DMAC7.html)
HU (1) HUE027350T2 (cg-RX-API-DMAC7.html)
PL (3) PL2695943T3 (cg-RX-API-DMAC7.html)
PT (2) PT1984506E (cg-RX-API-DMAC7.html)
SG (3) SG169390A1 (cg-RX-API-DMAC7.html)
WO (1) WO2007088371A2 (cg-RX-API-DMAC7.html)
ZA (2) ZA200806238B (cg-RX-API-DMAC7.html)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
GB0709061D0 (en) * 2007-05-11 2007-06-20 Avecia Biolog Ltd Expression system
GB0810154D0 (en) 2008-06-04 2008-07-09 Avecia Biolog Ltd Expression vector
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process
GB201018664D0 (en) 2010-11-05 2010-12-22 Msd Biolog Uk Ltd Expression process
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
GB201209896D0 (en) 2012-06-01 2012-07-18 Fujifilm Diosynth Biotechnologies Uk Ltd Process
TW201514193A (zh) 2013-01-31 2015-04-16 Glaxo Group Ltd 製備蛋白質之方法
US9873891B2 (en) * 2013-05-03 2018-01-23 Fujifilm Diosynth Biotechnologies Uk Limited Expression process
GB201311837D0 (en) 2013-07-01 2013-08-14 Fujifilm Diosynth Biotechnologies Uk Ltd Expression Process
GB201319930D0 (en) 2013-11-12 2013-12-25 Fujifilm Diosynth Biotechnologies Uk Ltd Expression process
JP6803328B2 (ja) * 2014-08-28 2021-01-06 シンセティック バイオロジクス，インコーポレイテッド 大腸菌ベースでのベータラクタマーゼ生産
US9791697B2 (en) 2015-12-11 2017-10-17 Panasonic Intellectual Property Management Co., Ltd. Screen
EP3436610A1 (en) * 2016-03-29 2019-02-06 Geltor, Inc. Expression of proteins in gram-negative bacteria wherein the ratio of periplasmic volume to cytoplasmic volume is between 0.5:1 and 10:1
BR112018072043A2 (pt) 2016-05-06 2019-02-12 Glaxosmithkline Intellectual Property Development Limited sequências de sinal e de ácido nucleico recombinantes, proteína de fusão recombinante, vetor de expressão recombinante, e, métodos para produzir uma proteína recombinante e controlar a viscosidade de um caldo de célula hospedeira e a expressão de uma proteína recombinante
GB201704659D0 (en) * 2017-03-24 2017-05-10 Fujifilm Diosynth Biotechnologies Uk Ltd Expression system
US20230151071A1 (en) * 2020-04-09 2023-05-18 United States Government As Represented By The Department Of Veterans Affairs Compositions comprising recombinant epo and methods of use thereof
US20230167467A1 (en) * 2020-04-15 2023-06-01 University of Alaska Anchorage Methods and compositions for the production of isobutene
CN118488963A (zh) 2021-12-17 2024-08-13 肯特大学 囊泡中生产重组生物分子的序列和方法

Family Cites Families (29)

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GB8517071D0 (en) * 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5059530A (en) * 1985-09-30 1991-10-22 Suntory Ltd. Expression vector for human TNF
JPS63267288A (ja) 1986-12-26 1988-11-04 Hoechst Japan Kk 増幅された遺伝子によつて蛋白質を生産する方法およびそのための遺伝子
US5510099A (en) * 1987-05-01 1996-04-23 Stratagene Mutagenesis testing using transgenic non-human animals carrying test DNA sequences
US5223407A (en) * 1988-08-31 1993-06-29 Allelix Inc. Excretion of heterologous proteins from e. coli
WO1991013979A1 (en) 1990-03-05 1991-09-19 President And Fellows Of Harvard College Transgenic mouse overexpressing il-4 and method of use
DE4029844A1 (de) * 1990-09-20 1992-03-26 Boehringer Mannheim Gmbh Carbamoylsarcosinhydrolase, kloniert
US5589392A (en) 1991-01-14 1996-12-31 Stratagene Nucleic acid construct encoding a nuclear transport peptide operatively linked to an inducible promoter
GB2253210B (en) * 1991-02-26 1995-06-07 Ici Plc Soluble heterologous polypeptide produced by culture of recombinant microorganisms
GB2253852B (en) 1991-02-26 1995-03-22 Ici Plc Vector for expression of heterologous polypeptide comprising inducible selection system
CA2130081A1 (en) 1992-02-14 1993-08-19 Jay M. Short Mutagenesis testing using transgenic non-human animals carrying test dna sequences
WO1998022578A1 (fr) 1996-11-15 1998-05-28 Institute Of Cytosignal Research, Inc. Procedes servant a detecter et a isoler des genes
WO1998022581A1 (fr) 1996-11-15 1998-05-28 Institute Of Cytosignal Research, Inc. Represseur de transcription
WO1999005279A1 (en) 1997-07-25 1999-02-04 Zeneca Limited Mcp-1 analogs
GB9715660D0 (en) * 1997-07-25 1997-10-01 Zeneca Ltd Proteins
EP1183357A2 (en) * 1999-05-20 2002-03-06 Scios Inc. Vascular endothelial growth factor dimers
EP1373297A4 (en) 2001-03-05 2005-09-21 Univ Virginia A LAC OPERATOR REPRESSOR SYSTEM
GB0113318D0 (en) 2001-06-01 2001-07-25 Lonza Biologics Plc Method for production of a protein
GB0227346D0 (en) * 2002-11-22 2002-12-31 Chiron Spa 741
AU2003299732A1 (en) 2002-12-18 2004-07-14 Genpath Pharmaceuticals, Incorporated Vectors for inducible rna interference
WO2005052151A1 (en) 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems
US20060177895A1 (en) * 2003-12-12 2006-08-10 Chung Chan Methods for enhancing expression of recombinant proteins
WO2005071089A2 (en) 2004-01-12 2005-08-04 Vertex Pharmaceuticals Incorporated Dual host expression vector system and screening methods
JP4854202B2 (ja) * 2004-03-31 2012-01-18 株式会社日本触媒 新規改変型ｓ−ヒドロキシニトリルリアーゼ
JP2005314252A (ja) * 2004-04-27 2005-11-10 Okayama Univ 糖尿病性腎症の予防・治療剤
JP2005341803A (ja) * 2004-05-31 2005-12-15 Institute Of Physical & Chemical Research 非対称な塩基配列を認識して切断する酵素Ｉ−ＣｓｍＩ及びその製造方法
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
RU2006129690A (ru) 2006-08-16 2008-02-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТЫ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА ydiN, ГЕНА ydiB ИЛИ ИХ КОМБИНАЦИИ
US9388425B2 (en) 2006-10-20 2016-07-12 Trustees Of Boston University Tunable genetic switch for regulating gene expression

Also Published As

Publication number Publication date
WO2007088371A3 (en) 2007-10-04
PT2386642E (pt) 2014-02-12
JP2017136072A (ja) 2017-08-10
JP6571707B2 (ja) 2019-09-04
JP2009525038A (ja) 2009-07-09
EP2386644B1 (en) 2015-12-16
KR101624345B1 (ko) 2016-06-07
EP2386642B1 (en) 2013-11-13
DK2695943T3 (en) 2016-04-18
EP2695943B1 (en) 2016-01-20
US11098335B2 (en) 2021-08-24
CN101410523A (zh) 2009-04-15
EP2386642A2 (en) 2011-11-16
EP2390334A2 (en) 2011-11-30
CN108841848B (zh) 2022-03-29
KR101512776B1 (ko) 2015-04-16
US20090170160A1 (en) 2009-07-02
SG169389A1 (en) 2011-03-30
JP5221385B2 (ja) 2013-06-26
EP2695943A1 (en) 2014-02-12
GB0602173D0 (en) 2006-03-15
DK2386644T3 (en) 2016-03-14
JP5713984B2 (ja) 2015-05-07
HUE027350T2 (hu) 2016-09-28
EP2386643A3 (en) 2012-11-28
EP2390334A3 (en) 2012-11-28
EP2386643A2 (en) 2011-11-16
JP2016136959A (ja) 2016-08-04
EP2386642A3 (en) 2012-06-13
SG169390A1 (en) 2011-03-30
PT1984506E (pt) 2013-09-06
CA2959915A1 (en) 2007-08-09
SG169388A1 (en) 2011-03-30
US20210348205A1 (en) 2021-11-11
PL2695943T3 (pl) 2016-07-29
EP2390334B1 (en) 2016-08-10
ZA201004904B (en) 2011-03-30
US20170240943A1 (en) 2017-08-24
ES2445173T3 (es) 2014-02-28
EP1984506A2 (en) 2008-10-29
CN108841848A (zh) 2018-11-20
KR20140119202A (ko) 2014-10-08
CN102690834B (zh) 2018-09-04
JP2013078323A (ja) 2013-05-02
EP2386644A3 (en) 2012-11-28
ES2436883T3 (es) 2014-01-07
PL1984506T3 (pl) 2013-11-29
JP6298481B2 (ja) 2018-03-20
KR20080098013A (ko) 2008-11-06
EP2386644A2 (en) 2011-11-16
EP2386643B1 (en) 2017-04-12
CN102690834A (zh) 2012-09-26
DK2386642T3 (en) 2014-02-17
JP2015006193A (ja) 2015-01-15
US9677103B2 (en) 2017-06-13
KR101492844B1 (ko) 2015-02-16
WO2007088371A2 (en) 2007-08-09
ZA200806238B (en) 2010-10-27
CA2637818A1 (en) 2007-08-09
CN101410523B (zh) 2012-05-16
CA2637818C (en) 2017-05-02
US20140065670A1 (en) 2014-03-06
KR20140090699A (ko) 2014-07-17
PL2386642T3 (pl) 2014-04-30
ES2569075T3 (es) 2016-05-06
US8530188B2 (en) 2013-09-10
EP1984506B1 (en) 2013-06-05
DK1984506T3 (da) 2013-09-16

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