CA2947426C - Multiplexed single cell gene expression analysis using template switch and tagmentation - Google Patents
Multiplexed single cell gene expression analysis using template switch and tagmentation Download PDFInfo
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- CA2947426C CA2947426C CA2947426A CA2947426A CA2947426C CA 2947426 C CA2947426 C CA 2947426C CA 2947426 A CA2947426 A CA 2947426A CA 2947426 A CA2947426 A CA 2947426A CA 2947426 C CA2947426 C CA 2947426C
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- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
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