CA2923706C - Methods and compositions for detecting mutation in the human ezh2 gene - Google Patents

Methods and compositions for detecting mutation in the human ezh2 gene Download PDF

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Publication number
CA2923706C
CA2923706C CA2923706A CA2923706A CA2923706C CA 2923706 C CA2923706 C CA 2923706C CA 2923706 A CA2923706 A CA 2923706A CA 2923706 A CA2923706 A CA 2923706A CA 2923706 C CA2923706 C CA 2923706C
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Prior art keywords
ezh2
nucleic acid
oligonucleotide
sequence
detecting
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CA2923706A
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English (en)
French (fr)
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CA2923706A1 (en
Inventor
Xiaoju Max Ma
Chitra MANOHAR
Alison Tsan
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA2923706A1 publication Critical patent/CA2923706A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
    • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/496—Non-condensed piperazines containing further heterocyclic rings, e.g. rifampin, thiothixene or sparfloxacin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/535—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with at least one nitrogen and one oxygen as the ring hetero atoms, e.g. 1,2-oxazines
    • A61K31/5375—1,4-Oxazines, e.g. morpholine
    • A61K31/5377—1,4-Oxazines, e.g. morpholine not condensed and containing further heterocyclic rings, e.g. timolol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Organic Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Oncology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hospice & Palliative Care (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA2923706A 2013-10-09 2014-10-07 Methods and compositions for detecting mutation in the human ezh2 gene Active CA2923706C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361888660P 2013-10-09 2013-10-09
US61/888,660 2013-10-09
PCT/EP2014/071372 WO2015052147A1 (en) 2013-10-09 2014-10-07 Methods and compositions for detecting mutation in the human ezh2 gene

Publications (2)

Publication Number Publication Date
CA2923706A1 CA2923706A1 (en) 2015-04-16
CA2923706C true CA2923706C (en) 2020-08-25

Family

ID=51663175

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2923706A Active CA2923706C (en) 2013-10-09 2014-10-07 Methods and compositions for detecting mutation in the human ezh2 gene

Country Status (7)

Country Link
US (1) US10260102B2 (enExample)
EP (1) EP3055422B1 (enExample)
JP (1) JP6619332B2 (enExample)
CN (1) CN105593378B (enExample)
CA (1) CA2923706C (enExample)
ES (1) ES2705573T3 (enExample)
WO (1) WO2015052147A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105593378B (zh) 2013-10-09 2019-09-20 豪夫迈·罗氏有限公司 用于在人ezh2基因中检测突变的方法和组合物
EA038337B1 (ru) 2014-06-17 2021-08-11 Эпизим, Инк. Ингибиторы ezh2 для лечения лимфомы
WO2016187394A1 (en) * 2015-05-19 2016-11-24 Van Wijnen Andre J Methods and materials for promoting bone formation
EP3674709A1 (en) 2015-11-05 2020-07-01 Epizyme Inc Flow cytometry for monitoring histone h3 methylation status
JP2019503391A (ja) * 2016-01-29 2019-02-07 エピザイム，インコーポレイティド 癌を処置するための併用療法
JP2019521988A (ja) 2016-06-17 2019-08-08 エピザイム，インコーポレイティド 癌を処置するためのｅｚｈ２阻害剤
CA3044691A1 (en) 2016-10-26 2018-05-03 Genea Biocells USA (Holdings), Inc. Improved generation of muscle lineage cells and therapeutic uses thereof
CN112226513B (zh) * 2020-10-29 2022-02-01 华中科技大学 一种检测ezh2基因可变剪切位点突变的引物、试剂盒及应用
CN119662835B (zh) * 2025-02-20 2025-05-27 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981176A (en) 1992-06-17 1999-11-09 City Of Hope Method of detecting and discriminating between nucleic acid sequences
DE69827060T2 (de) 1997-03-20 2005-03-24 F. Hoffmann-La Roche Ag Modifizierte Primer
WO2001042498A1 (en) 1999-12-10 2001-06-14 Toyo Boseki Kabushiki Kaisha Method of detecting nucleotide polymorphism
KR20060031809A (ko) * 2003-06-09 2006-04-13 더 리젠츠 오브 더 유니버시티 오브 미시간 암 치료 및 진단용 조성물 및 방법
JP2009247231A (ja) 2008-04-02 2009-10-29 Olympus Corp 標的塩基判別方法
JP5624044B2 (ja) 2008-10-20 2014-11-12 エフ．ホフマン−ラ ロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善されたアレル−特異的増幅
US20100143901A1 (en) 2008-12-09 2010-06-10 Roche Molecular Systems, Inc. Nuclease-Free Real-Time Detection of Nucleic Acids
CN101993934A (zh) 2009-08-10 2011-03-30 芮屈生物技术（上海）有限公司 一种ezh2基因的原位杂交检测试剂盒及其检测方法和应用
RU2014117632A (ru) * 2011-09-30 2015-11-10 ГЛЭКСОСМИТКЛАЙН ЭлЭлСи Способы лечения злокачественной опухоли
WO2013086639A1 (en) * 2011-12-14 2013-06-20 British Columbia Cancer Agency Branch Mutations indicative of weaver syndrome
CN105593378B (zh) 2013-10-09 2019-09-20 豪夫迈·罗氏有限公司 用于在人ezh2基因中检测突变的方法和组合物

Also Published As

Publication number Publication date
EP3055422B1 (en) 2018-11-14
ES2705573T3 (es) 2019-03-26
EP3055422A1 (en) 2016-08-17
JP2016533708A (ja) 2016-11-04
US10260102B2 (en) 2019-04-16
WO2015052147A1 (en) 2015-04-16
CN105593378A (zh) 2016-05-18
CA2923706A1 (en) 2015-04-16
CN105593378B (zh) 2019-09-20
US20150099747A1 (en) 2015-04-09
JP6619332B2 (ja) 2019-12-11

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