CA2917430C - Methods and compositions for detecting bacterial contamination - Google Patents

Methods and compositions for detecting bacterial contamination Download PDF

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Publication number
CA2917430C
CA2917430C CA2917430A CA2917430A CA2917430C CA 2917430 C CA2917430 C CA 2917430C CA 2917430 A CA2917430 A CA 2917430A CA 2917430 A CA2917430 A CA 2917430A CA 2917430 C CA2917430 C CA 2917430C
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Prior art keywords
seq
primer
sequence
amplification
sample
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CA2917430A
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English (en)
French (fr)
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CA2917430A1 (en
Inventor
James Jian Quan Wang
Xiangmin Cui
Hiunam CHAN
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DCH MOLECULAR DIAGNOSTICS Inc
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DCH MOLECULAR DIAGNOSTICS Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/15Modifications characterised by incorporating a consensus or conserved sequence
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00Reactions of nucleic acids characterised by
    • C12Q2531/10Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2917430A 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination Active CA2917430C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361858495P 2013-07-25 2013-07-25
US61/858,495 2013-07-25
PCT/US2014/047914 WO2015013465A2 (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Publications (2)

Publication Number Publication Date
CA2917430A1 CA2917430A1 (en) 2015-01-29
CA2917430C true CA2917430C (en) 2020-10-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2917430A Active CA2917430C (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Country Status (11)

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US (2) US10392670B2 (OSRAM)
EP (1) EP3024947B1 (OSRAM)
JP (3) JP2016525359A (OSRAM)
KR (1) KR102245110B1 (OSRAM)
CN (2) CN110592244A (OSRAM)
AU (1) AU2014293075A1 (OSRAM)
CA (1) CA2917430C (OSRAM)
MX (1) MX2016000997A (OSRAM)
SG (1) SG11201600550WA (OSRAM)
TW (1) TWI697560B (OSRAM)
WO (1) WO2015013465A2 (OSRAM)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2917430C (en) 2013-07-25 2020-10-06 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
EP3056567B1 (en) * 2013-10-07 2019-04-10 Mitsui Chemicals, Inc. Pcr primer set for bacterial dna amplification, kit for detecting and/or identifying bacterial species, and method for detecting and/or identifying bacterial species
US9953415B2 (en) * 2014-12-03 2018-04-24 Tata Consultancy Services Limited System and method for quantification of Escherichia coli bacteria in water
EP3334834B1 (en) * 2015-08-12 2020-05-13 Circulogene Theranostics, LLC Method of preparing cell free nucleic acid molecules by in situ amplification
CN105368961A (zh) * 2015-12-15 2016-03-02 天津脉络生物科技有限公司 一种沙雷菌定量检测荧光pcr试剂盒及其应用
CN105734164B (zh) * 2016-05-06 2019-05-17 苏州大学 一种检测细菌性脑膜炎病原体的多重pcr试剂盒
CN106434890B (zh) * 2016-08-30 2020-02-21 上海生物信息技术研究中心 快速恒温检测小肠结肠炎耶尔森氏菌的方法、引物及试剂盒
US20190062809A1 (en) * 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
EP3706574A4 (en) * 2017-11-06 2021-08-25 The Regents of The University of Michigan COMPOSITIONS AND METHODS ALLOWING THE DETECTION OF MULTIPLE MICROORGANISMS
KR20200081476A (ko) * 2017-11-13 2020-07-07 라이프 테크놀로지스 코포레이션 요로 미생물 검출을 위한 조성물, 방법 및 키트
US12291745B2 (en) * 2018-01-30 2025-05-06 Biofire Defense, Llc Methods and systems for validation of a nucleic acid amplification assay
CN108148834B (zh) * 2018-03-14 2018-11-13 北京理工大学 用于检测空间环境中的真菌的引物组合、试剂盒及方法
US20210172004A1 (en) * 2018-04-13 2021-06-10 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kit for fermentation monitoring
JP2022502027A (ja) * 2018-09-24 2022-01-11 セカンド ゲノム インコーポレイテッド 複数のbespokeスパイクイン混合物を介した、微生物叢の配列処理および示差的存在量分析を検証するための、組成物、システム、装置、および方法
CN109337967A (zh) * 2018-09-27 2019-02-15 华中科技大学鄂州工业技术研究院 一种实验室的微生物污染鉴别方法
FR3097560B1 (fr) * 2019-06-24 2021-06-25 Oreal Méthode de diagnostic d’une exposition chronique à la pollution
CN111209895B (zh) * 2020-02-14 2022-03-25 李淑媛 静脉药物配制样品的细菌污染检测系统、方法及存储介质
CN112575097B (zh) * 2020-10-12 2022-05-10 南开大学 一种用于检测蜡样芽孢杆菌的液相芯片及应用
WO2022081815A1 (en) * 2020-10-14 2022-04-21 Pickens Peter Vincent Optimized detection of blood-borne microbes
JP7553323B2 (ja) 2020-10-30 2024-09-18 株式会社Kri スタフィロコッカス細菌の同定方法
CN112226528B (zh) * 2020-12-14 2021-03-16 臻和(北京)生物科技有限公司 一种用于生物组织样本检测细菌污染的质检方法
KR20240107097A (ko) * 2021-09-03 2024-07-08 마이크로노마, 인크. 암 관련 미생물 바이오마커를 식별하는 방법
CN114480596A (zh) * 2022-02-11 2022-05-13 宁波市中心血站 一种微流控血液及血制品细菌污染检测方法

Family Cites Families (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
DE69028257T2 (de) 1989-07-11 1997-01-09 Gen Probe Inc Verfahren zur Vervielfältigung von Nukleinsäuresequenzen
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
WO1995013399A1 (en) 1993-11-12 1995-05-18 The Public Health Research Institute Of The City Of New York, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
CA2150986C (en) * 1994-06-17 1999-11-02 Mary Kathryn Meyer Oligonucleotide primers and probes for detection of bacteria
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
EP0892808B1 (en) 1996-04-12 2008-05-14 PHRI Properties, Inc. Detection probes, kits and assays
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
WO1998044151A1 (en) 1997-04-01 1998-10-08 Glaxo Group Limited Method of nucleic acid amplification
WO1999011813A2 (en) 1997-09-04 1999-03-11 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of use thereof
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
DE69940623D1 (de) 1998-11-09 2009-04-30 Eiken Chemical Prozess zur Synthetisierung von Nukleinsäure
NZ517121A (en) 1999-09-13 2004-05-28 Nugen Technologies Inc Methods and compositions for linear isothermal amplification of polynucleotide sequences
EP2322668A3 (en) 1999-09-28 2011-10-05 Geneohm Sciences Canada Inc. Highly conserved gene and its use to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes for microorganisms.
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
AUPQ909000A0 (en) * 2000-07-28 2000-08-24 University Of Sydney, The A method of detecting microorganisms
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
WO2004027025A2 (en) 2002-09-20 2004-04-01 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
AU2003288018A1 (en) 2002-11-20 2004-06-15 Pharmacia Italia S.P.A. Method for the detection of microorganisms in pharmaceutical products
WO2004050909A2 (en) * 2002-12-03 2004-06-17 Dartmouth College Method to measure a t cell response and its uses to qualify antigen-presenting cells
US20060046246A1 (en) * 2003-04-24 2006-03-02 Qiandong Zeng Genus, group, species and/or strain specific 16S rDNA sequences
CA2562221A1 (en) * 2005-10-21 2007-04-21 Canadian Blood Services A method for detection of staphylococcus epidermidis
US8017337B2 (en) 2007-04-19 2011-09-13 Molecular Detection, Inc. Methods, compositions and kits for detection and analysis of antibiotic-resistant bacteria
US8877060B2 (en) 2010-11-23 2014-11-04 Biovec Transfusion, Llc Methods for removing pathogens from a platelet preparation
CA2775273A1 (en) * 2009-10-22 2011-04-28 Biotools Biotechnological & Medical Laboratories, S.A. Composition, method and kit for detecting bacteria by means of sequencing
CA2917430C (en) 2013-07-25 2020-10-06 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination

Also Published As

Publication number Publication date
JP2016525359A (ja) 2016-08-25
CA2917430A1 (en) 2015-01-29
KR20160034318A (ko) 2016-03-29
TW201525145A (zh) 2015-07-01
WO2015013465A2 (en) 2015-01-29
JP2022023068A (ja) 2022-02-07
JP2019187431A (ja) 2019-10-31
MX2016000997A (es) 2016-04-19
SG11201600550WA (en) 2016-02-26
AU2014293075A1 (en) 2015-12-17
US10392670B2 (en) 2019-08-27
HK1219298A1 (en) 2017-03-31
EP3024947B1 (en) 2020-10-28
WO2015013465A3 (en) 2015-04-23
CN105473737A (zh) 2016-04-06
US20160355871A1 (en) 2016-12-08
KR102245110B1 (ko) 2021-04-27
EP3024947A4 (en) 2017-03-15
EP3024947A2 (en) 2016-06-01
US11274351B2 (en) 2022-03-15
TWI697560B (zh) 2020-07-01
CN110592244A (zh) 2019-12-20
US20200080134A1 (en) 2020-03-12
CN105473737B (zh) 2019-10-25

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