CA2886422C - Methods of quantitating heavy and light chain polypeptide pairs - Google Patents

Methods of quantitating heavy and light chain polypeptide pairs Download PDF

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Publication number
CA2886422C
CA2886422C CA2886422A CA2886422A CA2886422C CA 2886422 C CA2886422 C CA 2886422C CA 2886422 A CA2886422 A CA 2886422A CA 2886422 A CA2886422 A CA 2886422A CA 2886422 C CA2886422 C CA 2886422C
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Prior art keywords
light chain
heavy chain
chain polypeptide
polypeptide
heavy
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English (en)
French (fr)
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CA2886422A1 (en
Inventor
Jason Baardsnes
Maureen O'connor-Mccourt
Anders Ohrn
Adam Louis CORPER
Gordon Yiu Kon Ng
Antonios SAMIOTAKIS
Yang-Chieh CHOU
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Zymeworks BC Inc
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Zymeworks BC Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2886422A 2012-10-03 2013-10-03 Methods of quantitating heavy and light chain polypeptide pairs Active CA2886422C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261744911P 2012-10-03 2012-10-03
US61/744,911 2012-10-03
PCT/US2013/063306 WO2014055784A1 (en) 2012-10-03 2013-10-03 Methods of quantitating heavy and light chain polypeptide pairs

Publications (2)

Publication Number Publication Date
CA2886422A1 CA2886422A1 (en) 2014-04-10
CA2886422C true CA2886422C (en) 2022-12-13

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CA2886422A Active CA2886422C (en) 2012-10-03 2013-10-03 Methods of quantitating heavy and light chain polypeptide pairs

Country Status (7)

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US (1) US9771573B2 (cg-RX-API-DMAC7.html)
EP (1) EP2904093B1 (cg-RX-API-DMAC7.html)
JP (1) JP6581505B2 (cg-RX-API-DMAC7.html)
CN (1) CN105121630B (cg-RX-API-DMAC7.html)
AU (1) AU2013326974B2 (cg-RX-API-DMAC7.html)
CA (1) CA2886422C (cg-RX-API-DMAC7.html)
WO (1) WO2014055784A1 (cg-RX-API-DMAC7.html)

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Also Published As

Publication number Publication date
US20150211001A1 (en) 2015-07-30
EP2904093B1 (en) 2019-04-10
US9771573B2 (en) 2017-09-26
EP2904093A4 (en) 2016-06-08
EP2904093A1 (en) 2015-08-12
JP2015533089A (ja) 2015-11-19
CN105121630A (zh) 2015-12-02
AU2013326974B2 (en) 2019-01-03
CN105121630B (zh) 2018-09-25
CA2886422A1 (en) 2014-04-10
WO2014055784A1 (en) 2014-04-10
JP6581505B2 (ja) 2019-09-25
AU2013326974A1 (en) 2015-05-21

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