CA2881582A1 - Isolating traffic-enhancing mutants of drug delivery protein - Google Patents
Isolating traffic-enhancing mutants of drug delivery protein Download PDFInfo
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- CA2881582A1 CA2881582A1 CA2881582A CA2881582A CA2881582A1 CA 2881582 A1 CA2881582 A1 CA 2881582A1 CA 2881582 A CA2881582 A CA 2881582A CA 2881582 A CA2881582 A CA 2881582A CA 2881582 A1 CA2881582 A1 CA 2881582A1
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
- A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
- A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
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- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- C12N15/1034—Isolating an individual clone by screening libraries
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- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261679306P | 2012-08-03 | 2012-08-03 | |
| US61/679,306 | 2012-08-03 | ||
| PCT/US2013/053493 WO2014022811A1 (en) | 2012-08-03 | 2013-08-02 | Isolating traffic-enhancing mutants of drug delivery protein |
Publications (1)
| Publication Number | Publication Date |
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| CA2881582A1 true CA2881582A1 (en) | 2014-02-06 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CA2881582A Abandoned CA2881582A1 (en) | 2012-08-03 | 2013-08-02 | Isolating traffic-enhancing mutants of drug delivery protein |
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| Country | Link |
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| US (2) | US10036009B2 (https=) |
| EP (1) | EP2880160B1 (https=) |
| JP (2) | JP6618360B2 (https=) |
| KR (1) | KR102115630B1 (https=) |
| CN (1) | CN104619839A (https=) |
| AU (2) | AU2013296218B2 (https=) |
| BR (1) | BR112015002004A2 (https=) |
| CA (1) | CA2881582A1 (https=) |
| DK (1) | DK2880160T3 (https=) |
| ES (1) | ES2703052T3 (https=) |
| IL (1) | IL236955A0 (https=) |
| IN (1) | IN2015DN01196A (https=) |
| MX (1) | MX366347B (https=) |
| PL (1) | PL2880160T3 (https=) |
| PT (1) | PT2880160T (https=) |
| RU (1) | RU2015105052A (https=) |
| WO (1) | WO2014022811A1 (https=) |
| ZA (1) | ZA201500739B (https=) |
Families Citing this family (6)
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| WO2009009441A2 (en) | 2007-07-06 | 2009-01-15 | Cedars-Sinai Medical Center | Self-assembling complex for targeting chemical agents to cells |
| WO2010085665A2 (en) | 2009-01-23 | 2010-07-29 | Cedars-Sinai Medical Center | Targeted delivery system |
| CA2881582A1 (en) * | 2012-08-03 | 2014-02-06 | Cedars-Sinai Medical Center | Isolating traffic-enhancing mutants of drug delivery protein |
| DK2993983T3 (da) | 2013-05-08 | 2020-05-18 | Cedars Sinai Medical Center | Målretningskorroler til tumortoksicitet og mri |
| EP3096773B1 (en) | 2014-01-17 | 2020-07-01 | Cedars-Sinai Medical Center | Receptor targeting constructs and uses thereof |
| US9850293B2 (en) | 2014-04-04 | 2017-12-26 | Cedars-Sinai Medical Center | Targeting trastuzumab-resistant HER2+ breast cancer with a HER3-targeting nanoparticle |
Family Cites Families (33)
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|---|---|---|---|---|
| US5439829A (en) | 1991-01-30 | 1995-08-08 | Eli Lilly And Company | Immobilization of biologically active molecules by changing the Oxidation state of a chelated transition metal ion |
| US6287792B1 (en) | 1991-06-17 | 2001-09-11 | The Regents Of The University Of California | Drug delivery of antisense oligonucleotides and peptides to tissues in vivo and to cells using avidin-biotin technology |
| GB9223084D0 (en) | 1992-11-04 | 1992-12-16 | Imp Cancer Res Tech | Compounds to target cells |
| US5547945A (en) | 1993-07-15 | 1996-08-20 | Jcr Pharmaceuticals Co., Ltd. | Remitting agent for nephrotic syndrome and hepatopathy symptoms |
| US5559099A (en) * | 1994-09-08 | 1996-09-24 | Genvec, Inc. | Penton base protein and methods of using same |
| US6465253B1 (en) * | 1994-09-08 | 2002-10-15 | Genvec, Inc. | Vectors and methods for gene transfer to cells |
| US6066322A (en) | 1995-03-03 | 2000-05-23 | Millennium Pharmaceuticals, Inc. | Methods for the treatment of immune disorders |
| JP3420586B2 (ja) * | 1995-11-28 | 2003-06-23 | ジェンベク、インコーポレイティッド | 細胞への遣伝子導入のためのベクターおよび方法 |
| WO1998035036A1 (en) * | 1997-02-10 | 1998-08-13 | Genentech, Inc. | Heregulin variants |
| GB9709421D0 (en) | 1997-05-10 | 1997-07-02 | Zeneca Ltd | Chemical compounds |
| KR100609646B1 (ko) | 1998-08-18 | 2006-08-04 | 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 | Ｅｇｆ-ｒ 길항물질의 투여에 의한 기도 점액생산의 방지 |
| US6333396B1 (en) | 1998-10-20 | 2001-12-25 | Enzon, Inc. | Method for targeted delivery of nucleic acids |
| CA2391534A1 (en) | 1999-11-15 | 2001-05-25 | Drug Innovation & Design, Inc. | Selective cellular targeting: multifunctional delivery vehicles |
| CA2415319A1 (en) | 2000-06-16 | 2001-12-20 | Martek Biosciences Corporation | Recombinant phycobiliprotein and phycobiliprotein linker fusion proteins and uses therefore |
| IT1318704B1 (it) * | 2000-09-22 | 2003-08-27 | Consorzio Interuniversitario P | Vettori chimerici e loro uso per il trasferimento di geni eterologhi. |
| WO2002062823A2 (en) | 2001-02-02 | 2002-08-15 | Yale University | Peptides for facilitating composite receptor expression and translocation of macromolecules |
| EP1266963A1 (en) * | 2001-06-15 | 2002-12-18 | Crucell Holland B.V. | Chimaeric phages |
| SE0201863D0 (en) | 2002-06-18 | 2002-06-18 | Cepep Ab | Cell penetrating peptides |
| US7329638B2 (en) | 2003-04-30 | 2008-02-12 | The Regents Of The University Of Michigan | Drug delivery compositions |
| US7205387B2 (en) | 2003-08-28 | 2007-04-17 | Agency For Science, Technology And Research | Recombinant polypeptide useful for neurotrophin receptor mediated gene delivery and as neurotrophin agonist |
| US20060014712A1 (en) | 2004-05-30 | 2006-01-19 | Cemines, Inc. | Controlled delivery of therapeutic compounds |
| US8057821B2 (en) | 2004-11-03 | 2011-11-15 | Egen, Inc. | Biodegradable cross-linked cationic multi-block copolymers for gene delivery and methods of making thereof |
| US20110052697A1 (en) | 2006-05-17 | 2011-03-03 | Gwangju Institute Of Science & Technology | Aptamer-Directed Drug Delivery |
| JP4479755B2 (ja) | 2007-07-03 | 2010-06-09 | ソニー株式会社 | 有機エレクトロルミネッセンス素子、及び、有機エレクトロルミネッセンス表示装置 |
| WO2009009441A2 (en) * | 2007-07-06 | 2009-01-15 | Cedars-Sinai Medical Center | Self-assembling complex for targeting chemical agents to cells |
| MX2010005858A (es) * | 2007-11-28 | 2010-06-11 | Univ Pennsylvania | Adenovirus e simianos sadv-39, sadv-25.2, sadv-26, sadv-30, sadv-37 y sadv-38. |
| WO2010085665A2 (en) | 2009-01-23 | 2010-07-29 | Cedars-Sinai Medical Center | Targeted delivery system |
| WO2013052859A2 (en) | 2011-10-05 | 2013-04-11 | Genvec, Inc. | Adenoviral vector-based respiratory syncytial virus (rsv) vaccine |
| CA2881582A1 (en) * | 2012-08-03 | 2014-02-06 | Cedars-Sinai Medical Center | Isolating traffic-enhancing mutants of drug delivery protein |
| DK2993983T3 (da) | 2013-05-08 | 2020-05-18 | Cedars Sinai Medical Center | Målretningskorroler til tumortoksicitet og mri |
| EP3096773B1 (en) | 2014-01-17 | 2020-07-01 | Cedars-Sinai Medical Center | Receptor targeting constructs and uses thereof |
| US9850293B2 (en) | 2014-04-04 | 2017-12-26 | Cedars-Sinai Medical Center | Targeting trastuzumab-resistant HER2+ breast cancer with a HER3-targeting nanoparticle |
| EA201892797A1 (ru) * | 2016-05-27 | 2019-06-28 | Седарс-Синаи Медикал Сентер | Наночастицы для доставки лекарственных средств и способы лечения лекарственно-резистентного рака |
-
2013
- 2013-08-02 CA CA2881582A patent/CA2881582A1/en not_active Abandoned
- 2013-08-02 DK DK13826083.1T patent/DK2880160T3/en active
- 2013-08-02 EP EP13826083.1A patent/EP2880160B1/en not_active Not-in-force
- 2013-08-02 ES ES13826083T patent/ES2703052T3/es active Active
- 2013-08-02 PT PT13826083T patent/PT2880160T/pt unknown
- 2013-08-02 JP JP2015525636A patent/JP6618360B2/ja not_active Expired - Fee Related
- 2013-08-02 RU RU2015105052A patent/RU2015105052A/ru not_active Application Discontinuation
- 2013-08-02 BR BR112015002004A patent/BR112015002004A2/pt not_active Application Discontinuation
- 2013-08-02 PL PL13826083T patent/PL2880160T3/pl unknown
- 2013-08-02 WO PCT/US2013/053493 patent/WO2014022811A1/en not_active Ceased
- 2013-08-02 MX MX2015001366A patent/MX366347B/es active IP Right Grant
- 2013-08-02 AU AU2013296218A patent/AU2013296218B2/en not_active Ceased
- 2013-08-02 US US14/419,233 patent/US10036009B2/en active Active
- 2013-08-02 CN CN201380041215.1A patent/CN104619839A/zh active Pending
- 2013-08-02 KR KR1020157005438A patent/KR102115630B1/ko not_active Expired - Fee Related
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2015
- 2015-01-28 IL IL236955A patent/IL236955A0/en unknown
- 2015-02-02 ZA ZA2015/00739A patent/ZA201500739B/en unknown
- 2015-02-13 IN IN1196DEN2015 patent/IN2015DN01196A/en unknown
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2018
- 2018-06-25 US US16/017,866 patent/US10752893B2/en active Active
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2019
- 2019-07-04 AU AU2019204815A patent/AU2019204815A1/en not_active Abandoned
- 2019-07-18 JP JP2019132760A patent/JP2020002138A/ja active Pending
Also Published As
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| IL236955A0 (en) | 2015-03-31 |
| ES2703052T3 (es) | 2019-03-06 |
| PL2880160T3 (pl) | 2019-03-29 |
| MX366347B (es) | 2019-07-05 |
| US20150240231A1 (en) | 2015-08-27 |
| WO2014022811A1 (en) | 2014-02-06 |
| PT2880160T (pt) | 2018-12-17 |
| US20180298376A1 (en) | 2018-10-18 |
| AU2013296218A1 (en) | 2015-02-19 |
| US10036009B2 (en) | 2018-07-31 |
| JP2020002138A (ja) | 2020-01-09 |
| JP6618360B2 (ja) | 2019-12-11 |
| DK2880160T3 (en) | 2019-01-14 |
| ZA201500739B (en) | 2018-07-25 |
| RU2015105052A (ru) | 2016-09-27 |
| EP2880160B1 (en) | 2018-09-19 |
| IN2015DN01196A (https=) | 2015-06-26 |
| AU2013296218B2 (en) | 2019-07-18 |
| JP2015527343A (ja) | 2015-09-17 |
| KR102115630B1 (ko) | 2020-05-27 |
| EP2880160A4 (en) | 2016-04-20 |
| AU2019204815A1 (en) | 2019-07-25 |
| CN104619839A (zh) | 2015-05-13 |
| MX2015001366A (es) | 2015-08-13 |
| BR112015002004A2 (pt) | 2017-07-04 |
| US10752893B2 (en) | 2020-08-25 |
| KR20150038543A (ko) | 2015-04-08 |
| EP2880160A1 (en) | 2015-06-10 |
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| EEER | Examination request |
Effective date: 20180802 |
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| FZDE | Discontinued |
Effective date: 20211214 |