CA2879834A1 - Highly diverse combinatorial antibody libraries - Google Patents
Highly diverse combinatorial antibody libraries Download PDFInfo
- Publication number
- CA2879834A1 CA2879834A1 CA2879834A CA2879834A CA2879834A1 CA 2879834 A1 CA2879834 A1 CA 2879834A1 CA 2879834 A CA2879834 A CA 2879834A CA 2879834 A CA2879834 A CA 2879834A CA 2879834 A1 CA2879834 A1 CA 2879834A1
- Authority
- CA
- Canada
- Prior art keywords
- antibody
- library
- human
- families
- lambda
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000282414 Homo sapiens Species 0.000 claims abstract description 71
- 241000894007 species Species 0.000 claims abstract description 12
- 108090000623 proteins and genes Proteins 0.000 claims description 23
- 238000000034 method Methods 0.000 claims description 11
- 241000282842 Lama glama Species 0.000 claims description 10
- 235000002198 Annona diversifolia Nutrition 0.000 claims description 7
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 claims description 4
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims description 4
- 210000004602 germ cell Anatomy 0.000 abstract description 22
- 239000000427 antigen Substances 0.000 abstract description 12
- 108091007433 antigens Proteins 0.000 abstract description 12
- 102000036639 antigens Human genes 0.000 abstract description 12
- 230000005847 immunogenicity Effects 0.000 abstract 1
- 230000003053 immunization Effects 0.000 description 11
- 238000002649 immunization Methods 0.000 description 11
- 241001465754 Metazoa Species 0.000 description 10
- 230000003321 amplification Effects 0.000 description 9
- 238000003199 nucleic acid amplification method Methods 0.000 description 9
- 241001416177 Vicugna pacos Species 0.000 description 8
- 241000282836 Camelus dromedarius Species 0.000 description 7
- 239000013604 expression vector Substances 0.000 description 5
- 230000028993 immune response Effects 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 230000001225 therapeutic effect Effects 0.000 description 5
- 241001049453 Camelus ferus Species 0.000 description 4
- 241001529936 Murinae Species 0.000 description 4
- 239000002773 nucleotide Substances 0.000 description 4
- 125000003729 nucleotide group Chemical group 0.000 description 4
- 230000009824 affinity maturation Effects 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 238000002823 phage display Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 108091093088 Amplicon Proteins 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- 241000282840 Vicugna vicugna Species 0.000 description 2
- 230000000692 anti-sense effect Effects 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 210000000952 spleen Anatomy 0.000 description 2
- 241001244729 Apalis Species 0.000 description 1
- 208000000023 Bland White Garland Syndrome Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101001057612 Homo sapiens Dual specificity protein phosphatase 5 Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000282838 Lama Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 241001416176 Vicugna Species 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 201000004804 anomalous left coronary artery from the pulmonary artery Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 108010060686 dual specificity phosphatase 12 Proteins 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 102000043381 human DUSP5 Human genes 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 210000001165 lymph node Anatomy 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 210000005105 peripheral blood lymphocyte Anatomy 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 210000003705 ribosome Anatomy 0.000 description 1
- 238000002702 ribosome display Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
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- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261695819P | 2012-08-31 | 2012-08-31 | |
| US61/695,819 | 2012-08-31 | ||
| PCT/EP2013/068110 WO2014033304A2 (en) | 2012-08-31 | 2013-09-02 | Highly diverse combinatorial antibody libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2879834A1 true CA2879834A1 (en) | 2014-03-06 |
Family
ID=49117845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2879834A Abandoned CA2879834A1 (en) | 2012-08-31 | 2013-09-02 | Highly diverse combinatorial antibody libraries |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10040870B2 (enExample) |
| EP (1) | EP2890709A2 (enExample) |
| JP (1) | JP6283030B2 (enExample) |
| CN (1) | CN104583236A (enExample) |
| AU (1) | AU2013310839B2 (enExample) |
| CA (1) | CA2879834A1 (enExample) |
| IL (1) | IL236524B (enExample) |
| IN (1) | IN2015DN00138A (enExample) |
| WO (1) | WO2014033304A2 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111234027A (zh) | 2015-05-21 | 2020-06-05 | 哈普恩治疗公司 | 三特异性结合蛋白质及使用方法 |
| CN109641046B (zh) | 2016-05-20 | 2023-11-07 | 哈普恩治疗公司 | 单链可变片段cd3结合蛋白质 |
| BR112018073739A2 (pt) | 2016-05-20 | 2019-02-26 | Harpoon Therapeutics, Inc. | proteína de ligação de albumina sérica de domínio único |
| US11623958B2 (en) | 2016-05-20 | 2023-04-11 | Harpoon Therapeutics, Inc. | Single chain variable fragment CD3 binding proteins |
| CA3030099A1 (en) | 2016-07-08 | 2018-01-11 | Staten Biotechnology B.V. | Anti-apoc3 antibodies and methods of use thereof |
| JP7215997B2 (ja) * | 2016-11-23 | 2023-01-31 | ハープーン セラピューティクス，インク． | 前立腺特異的膜抗原（ｐｓｍａ）を標的とする三重特異性タンパク質と使用方法 |
| JP6812551B2 (ja) | 2016-11-23 | 2021-01-13 | ハープーン セラピューティクス，インク． | 前立腺特異的膜抗原結合タンパク質 |
| CN110139952B (zh) * | 2016-12-09 | 2022-11-25 | 深圳华大生命科学研究院 | 骆驼科抗体可变区免疫组库构建的引物组合及应用 |
| US11535668B2 (en) | 2017-02-28 | 2022-12-27 | Harpoon Therapeutics, Inc. | Inducible monovalent antigen binding protein |
| JP2020517242A (ja) | 2017-04-21 | 2020-06-18 | スターテン・バイオテクノロジー・ベー・フェー | 抗ＡｐｏＣ３抗体およびその使用方法 |
| CA3063362A1 (en) | 2017-05-12 | 2018-11-15 | Harpoon Therapeutics, Inc. | Msln targeting trispecific proteins and methods of use |
| CA3063359A1 (en) | 2017-05-12 | 2018-11-15 | Harpoon Therapeutics, Inc. | Mesothelin binding proteins |
| UA129446C2 (uk) | 2017-10-13 | 2025-04-30 | Гарпун Терап'Ютікс, Інк. | Триспецифічні білки і способи їх застосування |
| IL315737A (en) | 2017-10-13 | 2024-11-01 | Harpoon Therapeutics Inc | B-cell maturation antigen-binding proteins |
| BR112020008514A2 (pt) | 2017-10-31 | 2020-10-20 | Staten Biotechnology B.V. | anticorpos anti-apoc3 e métodos de uso dos mesmos |
| US10538583B2 (en) | 2017-10-31 | 2020-01-21 | Staten Biotechnology B.V. | Anti-APOC3 antibodies and compositions thereof |
| WO2019222283A1 (en) | 2018-05-14 | 2019-11-21 | Harpoon Therapeutics, Inc. | Binding moiety for conditional activation of immunoglobulin molecules |
| US12195544B2 (en) | 2018-09-21 | 2025-01-14 | Harpoon Therapeutics, Inc. | EGFR binding proteins and methods of use |
| EP3856771A4 (en) | 2018-09-25 | 2022-06-29 | Harpoon Therapeutics, Inc. | Dll3 binding proteins and methods of use |
| IL295448A (en) | 2020-02-21 | 2022-10-01 | Harpoon Therapeutics Inc | flt3 binding proteins and methods of use |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9722131D0 (en) | 1997-10-20 | 1997-12-17 | Medical Res Council | Method |
| GB2461546B (en) * | 2008-07-02 | 2010-07-07 | Argen X Bv | Antigen binding polypeptides |
| US8444976B2 (en) * | 2008-07-02 | 2013-05-21 | Argen-X B.V. | Antigen binding polypeptides |
| CN102459591B (zh) * | 2009-05-20 | 2015-05-13 | 诺维莫尼公司 | 合成性的多肽文库以及用于建立具有天然多样性的多肽变体的方法 |
| GB2476681B (en) | 2010-01-04 | 2012-04-04 | Argen X Bv | Humanized camelid VH, VK and VL immunoglobulin domains |
-
2013
- 2013-09-02 WO PCT/EP2013/068110 patent/WO2014033304A2/en not_active Ceased
- 2013-09-02 AU AU2013310839A patent/AU2013310839B2/en active Active
- 2013-09-02 CA CA2879834A patent/CA2879834A1/en not_active Abandoned
- 2013-09-02 JP JP2015529046A patent/JP6283030B2/ja active Active
- 2013-09-02 EP EP13758830.7A patent/EP2890709A2/en not_active Withdrawn
- 2013-09-02 CN CN201380044638.9A patent/CN104583236A/zh active Pending
- 2013-09-02 US US14/419,881 patent/US10040870B2/en active Active
- 2013-09-02 IN IN138DEN2015 patent/IN2015DN00138A/en unknown
-
2014
- 2014-12-31 IL IL236524A patent/IL236524B/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| JP6283030B2 (ja) | 2018-02-21 |
| US10040870B2 (en) | 2018-08-07 |
| EP2890709A2 (en) | 2015-07-08 |
| JP2015526495A (ja) | 2015-09-10 |
| WO2014033304A2 (en) | 2014-03-06 |
| US20150166680A1 (en) | 2015-06-18 |
| IN2015DN00138A (enExample) | 2015-06-12 |
| AU2013310839A1 (en) | 2015-01-29 |
| CN104583236A (zh) | 2015-04-29 |
| WO2014033304A3 (en) | 2014-05-01 |
| IL236524B (en) | 2019-01-31 |
| AU2013310839B2 (en) | 2017-06-01 |
| IL236524A0 (en) | 2015-02-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20180816 |
|
| FZDE | Discontinued |
Effective date: 20200831 |