CA2876343A1 - Recombinant dna elements for the expression of recombinant proteins in insects - Google Patents

Recombinant dna elements for the expression of recombinant proteins in insects Download PDF

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Publication number
CA2876343A1
CA2876343A1 CA2876343A CA2876343A CA2876343A1 CA 2876343 A1 CA2876343 A1 CA 2876343A1 CA 2876343 A CA2876343 A CA 2876343A CA 2876343 A CA2876343 A CA 2876343A CA 2876343 A1 CA2876343 A1 CA 2876343A1
Authority
CA
Canada
Prior art keywords
nucleic acid
insect
recombinant
acid sequence
baculovirus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2876343A
Other languages
English (en)
French (fr)
Inventor
Silvia Gomez Sebastian
Javier Lopez Vidal
Jose Angel Martinez Escribano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alternative Gene Expression SL ALGENEX
Original Assignee
Alternative Gene Expression SL ALGENEX
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alternative Gene Expression SL ALGENEX filed Critical Alternative Gene Expression SL ALGENEX
Publication of CA2876343A1 publication Critical patent/CA2876343A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/60—New or modified breeds of invertebrates
    • A01K67/61—Genetically modified invertebrates, e.g. transgenic or polyploid
    • A01K67/65—Genetically modified arthropods
    • A01K67/68—Genetically modified insects
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/70—Invertebrates
    • A01K2227/706—Insects, e.g. Drosophila melanogaster, medfly
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
CA2876343A 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in insects Abandoned CA2876343A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201161495467P 2011-06-10 2011-06-10
EP11169634.0 2011-06-10
EP11169634 2011-06-10
US61/495,467 2011-06-10
EP2011068381 2011-10-20
EPPCT/EP2011/068381 2011-10-20
EPPCT/EP2011/072406 2011-12-12
EP2011072406 2011-12-12
PCT/EP2012/061088 WO2012168493A2 (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in insects

Publications (1)

Publication Number Publication Date
CA2876343A1 true CA2876343A1 (en) 2012-12-13

Family

ID=47296540

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2876343A Abandoned CA2876343A1 (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in insects
CA2876641A Active CA2876641C (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in a host cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2876641A Active CA2876641C (en) 2011-06-10 2012-06-12 Recombinant dna elements for the expression of recombinant proteins in a host cell

Country Status (8)

Country Link
EP (2) EP2858489B1 (enExample)
CA (2) CA2876343A1 (enExample)
DK (2) DK2858490T5 (enExample)
IN (1) IN2014MN02500A (enExample)
MX (1) MX353498B (enExample)
PL (2) PL2858489T3 (enExample)
PT (2) PT2858489T (enExample)
WO (6) WO2012168493A2 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103749393A (zh) * 2013-12-20 2014-04-30 铜陵桂生生态养殖有限公司 一种降低蝎子死亡率的养殖方法
US11278014B2 (en) 2015-09-17 2022-03-22 Alternative Gene Expression, S.L. Expression of recombinant proteins in Trichoplusia ni pupae

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105392362B (zh) 2012-06-12 2018-11-09 替代基因表达公司 用于在宿主昆虫中表达重组蛋白的杆状病毒dna元件
US9982239B2 (en) 2012-06-12 2018-05-29 Alternative Gene Expression S.L. Baculoviral DNA elements for the expression of recombinant proteins in a host cell
ES2611302T3 (es) * 2012-06-12 2017-05-08 Alternative Gene Expression, S.L. Elementos de ADN recombinante para la expresión de proteínas recombinantes en insectos
ES2554561B1 (es) * 2014-06-16 2016-09-30 Universitat De València Nuevos promotores derivados de baculovirus con elevada actividad en sistemas de expresión basados en baculovirus
CN105087606A (zh) * 2015-09-23 2015-11-25 天津瑞普生物技术股份有限公司 猪圆环病毒2型cap抗原制备方法
CN105238816B (zh) * 2015-10-26 2018-08-24 广州汉腾生物科技有限公司 一种表达载体
US11572390B2 (en) 2017-03-27 2023-02-07 The University Of Queensland Chimeric insect-specific flaviviruses
ES2921999T3 (es) 2018-06-20 2022-09-06 Fatro Spa Vacunas para la prevención de la enfermedad hemorrágica del conejo
CN110964749A (zh) * 2018-09-29 2020-04-07 普莱柯生物工程股份有限公司 一种在杆状病毒表达系统中高效表达外源蛋白的方法及其应用
WO2026120074A1 (en) 2024-12-05 2026-06-11 Alternative Gene Expression S.L. Sedoreoviridae vaccines based on truncated vp2 capsid proteins
WO2026139625A1 (en) 2024-12-26 2026-07-02 Alternative Gene Expression S.L. Glutamate-oxaloacetate transaminase (got1) – fc (fragment crystallizable) fusion proteins

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1377395A (en) * 1993-12-23 1995-07-10 University Technologies International Inc. Methods of expressing proteins in insect cells and methods of killing insects
US5965393A (en) * 1997-07-01 1999-10-12 National Institute Of Immunology Method for enhancing foreign gene expression in baculovirus expression vector system
RU2158505C2 (ru) * 1998-06-09 2000-11-10 Совхоз "Тепличный" Способ приготовления углеводного корма для насекомых-опылителей
ES2232308B1 (es) 2003-11-12 2006-10-16 Laboratorios Calier, S.A. Dispositivo para la cria de larvas.
JP4271122B2 (ja) * 2004-10-15 2009-06-03 財団法人 ひろしま産業振興機構 カイコでの組換えタンパク質製造のためのポリヌクレオチド
US8148129B2 (en) * 2006-06-30 2012-04-03 The Regents Of The University Of California Generation of potent dominant negative transcriptional inhibitors
US8105827B2 (en) * 2007-09-06 2012-01-31 Academia Sinica Protein expression systems
KR20110081810A (ko) * 2008-09-02 2011-07-14 얼터너티브 진 엑스프레션 에스.엘.(얼제넥스) 곤충 세포에서의 외래 단백질 발현을 위한 곤충-유래 프로모터

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103749393A (zh) * 2013-12-20 2014-04-30 铜陵桂生生态养殖有限公司 一种降低蝎子死亡率的养殖方法
US11278014B2 (en) 2015-09-17 2022-03-22 Alternative Gene Expression, S.L. Expression of recombinant proteins in Trichoplusia ni pupae

Also Published As

Publication number Publication date
WO2012168492A2 (en) 2012-12-13
WO2012168493A2 (en) 2012-12-13
PT2858490T (pt) 2016-11-28
WO2012169939A9 (en) 2013-02-14
MX2014015277A (es) 2015-12-15
WO2012168493A8 (en) 2013-04-04
DK2858490T5 (en) 2017-01-23
WO2012169940A9 (en) 2013-02-14
WO2012168789A3 (en) 2013-05-10
PT2858489T (pt) 2016-12-14
WO2012168493A3 (en) 2013-05-23
EP2858489B1 (en) 2016-11-09
WO2012168789A2 (en) 2012-12-13
EP2858490A2 (en) 2015-04-15
WO2012168492A3 (en) 2013-04-11
WO2012169939A2 (en) 2012-12-13
WO2012169939A3 (en) 2013-05-10
EP2858490B1 (en) 2016-10-26
CA2876641A1 (en) 2012-12-13
WO2012168788A3 (en) 2013-05-10
WO2012168788A2 (en) 2012-12-13
BR112014031039A2 (pt) 2018-05-08
IN2014MN02500A (enExample) 2015-07-17
WO2012169940A2 (en) 2012-12-13
WO2012169940A3 (en) 2013-04-04
PL2858489T3 (pl) 2017-07-31
EP2858489A2 (en) 2015-04-15
DK2858489T3 (da) 2017-01-02
PL2858490T3 (pl) 2017-08-31
DK2858490T3 (en) 2016-12-12
MX353498B (es) 2018-01-16
CA2876641C (en) 2018-09-25

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20170131

FZDE Discontinued

Effective date: 20210831