CA2876178C - Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel - Google Patents
Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel Download PDFInfo
- Publication number
- CA2876178C CA2876178C CA2876178A CA2876178A CA2876178C CA 2876178 C CA2876178 C CA 2876178C CA 2876178 A CA2876178 A CA 2876178A CA 2876178 A CA2876178 A CA 2876178A CA 2876178 C CA2876178 C CA 2876178C
- Authority
- CA
- Canada
- Prior art keywords
- microorganism
- biodiesel
- clostridium
- nucleic acid
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
- C12P7/649—Biodiesel, i.e. fatty acid alkyl esters
-
- C—CHEMISTRY; METALLURGY
- C10—PETROLEUM, GAS OR COKE INDUSTRIES; TECHNICAL GASES CONTAINING CARBON MONOXIDE; FUELS; LUBRICANTS; PEAT
- C10L—FUELS NOT OTHERWISE PROVIDED FOR; NATURAL GAS; SYNTHETIC NATURAL GAS OBTAINED BY PROCESSES NOT COVERED BY SUBCLASSES C10G OR C10K; LIQUIFIED PETROLEUM GAS; USE OF ADDITIVES TO FUELS OR FIRES; FIRE-LIGHTERS
- C10L1/00—Liquid carbonaceous fuels
- C10L1/02—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only
- C10L1/026—Liquid carbonaceous fuels essentially based on components consisting of carbon, hydrogen, and oxygen only for compression ignition
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01075—Long-chain-alcohol O-fatty-acyltransferase (2.3.1.75)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/145—Clostridium
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261662467P | 2012-06-21 | 2012-06-21 | |
| US61/662,467 | 2012-06-21 | ||
| PCT/NZ2013/000108 WO2013191567A1 (en) | 2012-06-21 | 2013-06-21 | Recombinant microorganisms make biodiesel |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2876178A1 CA2876178A1 (en) | 2013-12-27 |
| CA2876178C true CA2876178C (en) | 2017-02-28 |
Family
ID=49769067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2876178A Active CA2876178C (en) | 2012-06-21 | 2013-06-21 | Recombinant microorganisms expressing a non-specific acyltransferase make biodiesel |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US9347076B2 (en, 2012) |
| EP (1) | EP2864470B2 (en, 2012) |
| JP (1) | JP6466836B2 (en, 2012) |
| KR (1) | KR102097844B1 (en, 2012) |
| CN (1) | CN104640977B (en, 2012) |
| BR (1) | BR112014031876B1 (en, 2012) |
| CA (1) | CA2876178C (en, 2012) |
| ES (1) | ES2655214T5 (en, 2012) |
| IN (1) | IN2014DN10507A (en, 2012) |
| MY (1) | MY164882A (en, 2012) |
| NO (1) | NO2953671T3 (en, 2012) |
| PL (1) | PL2864470T5 (en, 2012) |
| WO (1) | WO2013191567A1 (en, 2012) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201112231D0 (en) | 2011-07-15 | 2011-08-31 | Verdant Bioproducts Ltd | Micro-organism |
| US20130323820A1 (en) | 2012-06-01 | 2013-12-05 | Lanzatech New Zealand Limited | Recombinant microorganisms and uses therefor |
| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
| EP3099779B1 (en) | 2014-01-28 | 2020-09-02 | LanzaTech New Zealand Limited | Method of producing a recombinant microorganism |
| JP6309304B2 (ja) * | 2014-02-24 | 2018-04-11 | 積水化学工業株式会社 | 組換え細胞、並びに、１，４−ブタンジオールの生産方法 |
| JP6662868B2 (ja) | 2014-10-22 | 2020-03-11 | ランザテク・ニュージーランド・リミテッド | 複数ステージバイオリアクタープロセス |
| EP4180806A1 (en) | 2014-10-22 | 2023-05-17 | LanzaTech NZ, Inc. | Bioreactor and gas testing unit and method |
| TWI739734B (zh) | 2015-02-23 | 2021-09-21 | 紐西蘭商藍瑟科技紐西蘭有限公司 | 用於將甲烷轉化為產物之重組產醋酸細菌 |
| CA3051234C (en) | 2015-10-13 | 2020-09-15 | Lanzatech New Zealand Limited | Genetically engineered bacterium comprising energy-generating fermentation pathway |
| WO2017096324A1 (en) | 2015-12-03 | 2017-06-08 | Lanzatech New Zealand Limited | Arginine supplementation to improve efficiency in gas fermenting acetogens |
| US10358661B2 (en) | 2015-12-28 | 2019-07-23 | Lanzatech New Zealand Limited | Microorganism with modified hydrogenase activity |
| WO2017136478A1 (en) | 2016-02-01 | 2017-08-10 | Lanzatech New Zealand Limited | Integrated fermentation and electrolysis process |
| DK3420089T3 (da) | 2016-02-26 | 2022-03-14 | Lanzatech Nz Inc | CRISPR/Cas-systemer til C-1-fikserende bakterier |
| EP3464559A4 (en) | 2016-05-14 | 2020-02-12 | Lanzatech, Inc. | MODIFIED ALDEHYDE / FERREDOXIN OXYDOREDUCTASE MICROORGANISM AND RELATED METHODS |
| CN107217017B (zh) * | 2017-05-27 | 2020-12-08 | 青岛农业大学 | 一株不动杆菌及其在石油降解中的应用 |
| KR102698275B1 (ko) | 2017-09-08 | 2024-08-22 | 란자테크, 인크. | 수소 풍부 c1-함유 기질을 사용하는 대사산물 생산 공정 및 시스템 |
| KR102766204B1 (ko) | 2017-12-19 | 2025-02-10 | 란자테크, 인크. | 에틸렌 글리콜의 생물학적 생성을 위한 미생물 및 방법 |
| KR102799155B1 (ko) | 2018-02-12 | 2025-04-21 | 란자테크, 인크. | 탄소 전환 효율을 개선하기 위한 공정 |
| WO2019204029A1 (en) | 2018-04-20 | 2019-10-24 | Lanzatech, Inc. | Intermittent electrolysis streams |
| CA3120151C (en) | 2018-11-19 | 2024-04-30 | Lanzatech, Inc. | Integration of fermentation and gasification |
| KR102702211B1 (ko) | 2019-02-08 | 2024-09-02 | 란자테크, 인크. | 유사한 비등점 생성물의 회수 방법 |
| BR112021021672A2 (pt) | 2019-07-11 | 2022-02-08 | Lanzatech Inc | Método para otimizar o fluxo de gás para processos de fermentação paralelos, fermentação em série, e, fermentação |
| CN111088193B (zh) * | 2020-01-10 | 2022-09-09 | 南京工业大学 | 一种提高食甲基丁酸杆菌电转频率的方法 |
| US11932818B2 (en) | 2020-03-16 | 2024-03-19 | Lanzatech, Inc. | Tail gas of gas fermentation to dry gasification feedstock |
| KR20220140619A (ko) | 2020-03-18 | 2022-10-18 | 란자테크, 인크. | 가스성 기질로부터의 2-페닐에탄올의 발효 제조 |
| JP2023523343A (ja) | 2020-04-29 | 2023-06-02 | ランザテク，インコーポレイテッド | ガス基質からのβ－ケトアジペートの発酵生成 |
| AU2021284451B2 (en) | 2020-06-06 | 2024-01-11 | Lanzatech, Inc. | Microorganism with knock-in at acetolactate decarboxylase gene locus |
| CN116348603B (zh) | 2021-02-08 | 2024-07-02 | 朗泽科技有限公司 | 重组微生物及其用途 |
| CA3213232A1 (en) | 2021-04-09 | 2022-10-13 | Robert John CONRADO | Process and apparatus for providing a feedstock |
| WO2023004293A1 (en) | 2021-07-20 | 2023-01-26 | Lanzatech, Inc. | Recombinant microorganisms and uses therefor |
| TW202307202A (zh) | 2021-08-06 | 2023-02-16 | 美商朗澤科技有限公司 | 用於改良乙二醇之生物產生的微生物及方法 |
| US12091648B2 (en) | 2021-11-03 | 2024-09-17 | Lanzatech, Inc. | System and method for generating bubbles in a vessel |
| US12280331B2 (en) | 2022-04-29 | 2025-04-22 | Lanzatech, Inc. | Low residence time gas separator |
| US12077800B2 (en) | 2022-06-16 | 2024-09-03 | Lanzatech, Inc. | Liquid distributor system and process of liquid distribution |
| WO2023250382A1 (en) | 2022-06-21 | 2023-12-28 | Lanzatech, Inc. | Microorganisms and methods for the continuous co-production of tandem repeat proteins and chemical products from c1-substrates |
| WO2023250389A1 (en) | 2022-06-21 | 2023-12-28 | Lanzatech, Inc. | Microorganisms and methods for the continuous co-production of high-value, specialized proteins and chemical products from c1-substrates |
| US12129503B2 (en) | 2022-08-10 | 2024-10-29 | Lanzatech, Inc. | Carbon sequestration in soils with production of chemical products |
| US12359224B2 (en) | 2023-06-05 | 2025-07-15 | Lanzatech, Inc. | Integrated gas fermentation and carbon black processes |
| US12281344B2 (en) | 2023-06-05 | 2025-04-22 | Lanzatech, Inc. | Integrated gas fermentation |
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| US5173429A (en) | 1990-11-09 | 1992-12-22 | The Board Of Trustees Of The University Of Arkansas | Clostridiumm ljungdahlii, an anaerobic ethanol and acetate producing microorganism |
| US5593886A (en) | 1992-10-30 | 1997-01-14 | Gaddy; James L. | Clostridium stain which produces acetic acid from waste gases |
| UA72220C2 (uk) | 1998-09-08 | 2005-02-15 | Байоенджініерінг Рісорсиз, Інк. | Незмішувана з водою суміш розчинник/співрозчинник для екстрагування оцтової кислоти, спосіб одержання оцтової кислоти (варіанти), спосіб анаеробного мікробного бродіння для одержання оцтової кислоти (варіанти), модифікований розчинник та спосіб його одержання |
| UA76117C2 (en) * | 2000-07-25 | 2006-07-17 | Emmaus Foundation Inc | A process for a stable producing ethanol |
| DE102004052115A1 (de) * | 2004-10-26 | 2006-04-27 | Westfälische Wilhelms-Universität Münster | Mikroorganismen zur Herstellung von Fettsäureestern, Verfahren zu deren Herstellung und deren Verwendung |
| MX344588B (es) † | 2006-05-19 | 2016-12-20 | Reg Life Sciences Llc | Produccion de acidos grasos y derivados de los mismos. |
| US7704723B2 (en) | 2006-08-31 | 2010-04-27 | The Board Of Regents For Oklahoma State University | Isolation and characterization of novel clostridial species |
| BRPI0809345A2 (pt) * | 2007-03-28 | 2014-10-07 | Ls9 Inc | Células recombinantes, composições produzidas por estas, métodos para produzir derivados de ácidos graxos em uma célula recombinante, para aumentar a produção de derivados de ácidos graxos em uma célula recombinante, construto recombinante, vetores, métodos para aumentar a produção de derivados de ácidos graxos em uma célula hospedeira de produção, para produzir derivados de ácidos graxos, derivados de ácidos graxos, composições de biocombustível. |
| US20100199548A1 (en) * | 2007-07-06 | 2010-08-12 | Ls9, Inc. | Systems and methods for the production of fatty esters |
| KR101375029B1 (ko) | 2007-11-13 | 2014-03-14 | 란자테크 뉴질랜드 리미티드 | 신규 세균 및 이의 이용 방법 |
| GB0801787D0 (en) * | 2008-01-31 | 2008-03-05 | Reclaim Resources Ltd | Apparatus and method for treating waste |
| US8048654B2 (en) * | 2010-06-09 | 2011-11-01 | Joule Unlimited Technologies, Inc. | Methods and compositions for the recombinant biosynthesis of fatty acids and esters |
| WO2010022090A1 (en) * | 2008-08-18 | 2010-02-25 | Ls9, Inc. | Systems and methods for the production of mixed fatty esters |
| BRPI1014952A2 (pt) * | 2009-04-10 | 2019-09-24 | Ls9 Inc | "composição produzida por um microrganismo e composição de biocombustível." |
| WO2010127318A2 (en) | 2009-05-01 | 2010-11-04 | The Regents Of The University Of California | Product of fatty acid esters from biomass polymers |
| US8143037B2 (en) | 2010-03-19 | 2012-03-27 | Coskata, Inc. | Ethanologenic Clostridium species, Clostridium coskatii |
| CN103282505A (zh) * | 2010-08-26 | 2013-09-04 | 新西兰郎泽科技公司 | 通过发酵产生乙醇和乙烯的方法 |
| US20110236941A1 (en) | 2010-10-22 | 2011-09-29 | Lanzatech New Zealand Limited | Recombinant microorganism and methods of production thereof |
| KR102015672B1 (ko) | 2011-02-25 | 2019-08-29 | 란자테크 뉴질랜드 리미티드 | 재조합 미생물 및 이의 용도 |
| CN102492672A (zh) * | 2011-11-26 | 2012-06-13 | 宁波大学 | 一种催化二酰甘油酰基化的酶 |
| US9347076B2 (en) | 2012-06-21 | 2016-05-24 | Lanzatech New Zealand Limited | Recombinant microorganisms that make biodiesel |
-
2013
- 2013-06-20 US US13/923,352 patent/US9347076B2/en active Active
- 2013-06-21 EP EP13806177.5A patent/EP2864470B2/en active Active
- 2013-06-21 MY MYPI2014003406A patent/MY164882A/en unknown
- 2013-06-21 PL PL13806177T patent/PL2864470T5/pl unknown
- 2013-06-21 ES ES13806177T patent/ES2655214T5/es active Active
- 2013-06-21 CA CA2876178A patent/CA2876178C/en active Active
- 2013-06-21 KR KR1020157000238A patent/KR102097844B1/ko active Active
- 2013-06-21 WO PCT/NZ2013/000108 patent/WO2013191567A1/en active Application Filing
- 2013-06-21 IN IN10507DEN2014 patent/IN2014DN10507A/en unknown
- 2013-06-21 BR BR112014031876-0A patent/BR112014031876B1/pt active IP Right Grant
- 2013-06-21 JP JP2015518359A patent/JP6466836B2/ja active Active
- 2013-06-21 CN CN201380044451.9A patent/CN104640977B/zh active Active
-
2014
- 2014-02-05 NO NO14703269A patent/NO2953671T3/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2655214T5 (es) | 2021-10-11 |
| PL2864470T5 (pl) | 2021-05-17 |
| WO2013191567A1 (en) | 2013-12-27 |
| PL2864470T3 (pl) | 2018-05-30 |
| KR20150023651A (ko) | 2015-03-05 |
| CN104640977A (zh) | 2015-05-20 |
| IN2014DN10507A (en, 2012) | 2015-08-21 |
| JP6466836B2 (ja) | 2019-02-06 |
| CN104640977B (zh) | 2019-04-12 |
| KR102097844B1 (ko) | 2020-04-06 |
| EP2864470B2 (en) | 2020-12-23 |
| US20130344547A1 (en) | 2013-12-26 |
| EP2864470B1 (en) | 2017-10-04 |
| CA2876178A1 (en) | 2013-12-27 |
| NO2953671T3 (en, 2012) | 2018-03-17 |
| JP2015519918A (ja) | 2015-07-16 |
| BR112014031876B1 (pt) | 2022-05-10 |
| EP2864470A4 (en) | 2015-12-16 |
| MY164882A (en) | 2018-01-30 |
| ES2655214T3 (es) | 2018-02-19 |
| BR112014031876A2 (pt) | 2017-08-01 |
| US9347076B2 (en) | 2016-05-24 |
| EP2864470A1 (en) | 2015-04-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20141209 |